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The map label for this gene is 77460029

Identifier: 77460029

GI number: 77460029

Start: 4308431

End: 4309090

Strand: Reverse

Name: 77460029

Synonym: Pfl01_3807

Alternate gene names: NA

Gene position: 4309090-4308431 (Counterclockwise)

Preceding gene: 77460037

Following gene: 77460028

Centisome position: 66.93

GC content: 60.76

Gene sequence:

>660_bases
ATGAAAAAGCTTTTTACAATTATGGCGCTGATTGCGCTGACGGTCGGTAGCATCGGCCTGAGTTCGGCGCGCCAGTCGTC
CGCCAGCAAGGCGGAATCGGTGGCGGGGGTATTCGATTACTACCTGCTGACGTTGTCCTGGTCGCCGACCTTTTGCCTGA
CCCACAAGGACGACCCGCAATGCACCGGCAAGGGCTATGGTTTTGTCCTGCATGGCCTGTGGCCGCAATACGCCAAGGGC
GGCTGGCCGGAATCCTGCCCGCCGCTGACCAACCTGTCCGCCGCCGAAACAGCTAAAGGCCTGACGCTGTTCCCGACCAA
AAAACTCCTCGATCACGAATGGAGCAAGCACGGCACTTGCAGCGGCCTCGGCGCGATGGGCTATCTGGACGAAGCGGACA
AAGCGGTGGGCGCGGTGAAAATCCCGCAAGAGTTGCAGCCGTTCAGTAGCTCTTATTACTTCGAAGCGCAGGAAATCGCC
GATCTGTTCCGCAGGAGCAATCCGGGCATCCCGTCAGACGGCATCGCCGTGATCTGCAACGGCCCGGAACTCTCGGAAGT
GCGCGTGTGCATGGGCAAGGACCTGCAATTCGGCGCCTGCGGCAAGGGCGTGAAAACCCAGTGCCGGGCGGGGGATATTC
GAGTACCGCCGTCGCGATAA

Upstream 100 bases:

>100_bases
CGTCATGCGCTTGCAACACGGGCATGACTCAATCAGCGTCTGGCCAAGTGATGGTCAGACCACAGAAACCACGGACACAC
GCGTCCCAAGGAACGGGAAC

Downstream 100 bases:

>100_bases
CGCAATCGGTTACTGGCCATAAAAAACAGGCGCCTTTTCAGGCGCCTGTTTTTTTGCAATCGGGTTTGATCAATGGAAGG
ACTTTGTTCATGCTGCGCGC

Product: ribonuclease T2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKKLFTIMALIALTVGSIGLSSARQSSASKAESVAGVFDYYLLTLSWSPTFCLTHKDDPQCTGKGYGFVLHGLWPQYAKG
GWPESCPPLTNLSAAETAKGLTLFPTKKLLDHEWSKHGTCSGLGAMGYLDEADKAVGAVKIPQELQPFSSSYYFEAQEIA
DLFRRSNPGIPSDGIAVICNGPELSEVRVCMGKDLQFGACGKGVKTQCRAGDIRVPPSR

Sequences:

>Translated_219_residues
MKKLFTIMALIALTVGSIGLSSARQSSASKAESVAGVFDYYLLTLSWSPTFCLTHKDDPQCTGKGYGFVLHGLWPQYAKG
GWPESCPPLTNLSAAETAKGLTLFPTKKLLDHEWSKHGTCSGLGAMGYLDEADKAVGAVKIPQELQPFSSSYYFEAQEIA
DLFRRSNPGIPSDGIAVICNGPELSEVRVCMGKDLQFGACGKGVKTQCRAGDIRVPPSR
>Mature_219_residues
MKKLFTIMALIALTVGSIGLSSARQSSASKAESVAGVFDYYLLTLSWSPTFCLTHKDDPQCTGKGYGFVLHGLWPQYAKG
GWPESCPPLTNLSAAETAKGLTLFPTKKLLDHEWSKHGTCSGLGAMGYLDEADKAVGAVKIPQELQPFSSSYYFEAQEIA
DLFRRSNPGIPSDGIAVICNGPELSEVRVCMGKDLQFGACGKGVKTQCRAGDIRVPPSR

Specific function: One of the few RNases that cleave the phosphodiester bond between any two nucleotide. Shows a preference for adenylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=An RNase I-like form (periplasmic) and RNase I*-like form (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: NA

Molecular weight: Translated: 23529; Mature: 23529

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFTIMALIALTVGSIGLSSARQSSASKAESVAGVFDYYLLTLSWSPTFCLTHKDDPQ
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCC
CTGKGYGFVLHGLWPQYAKGGWPESCPPLTNLSAAETAKGLTLFPTKKLLDHEWSKHGTC
CCCCCCEEEEECCCHHHHCCCCCCCCCCCCCCCHHHHCCCCEECCCHHHHHCCCCCCCCC
SGLGAMGYLDEADKAVGAVKIPQELQPFSSSYYFEAQEIADLFRRSNPGIPSDGIAVICN
CCCCCCCCHHHHHHHHCEEECCHHHCCCCCCCEECHHHHHHHHHHCCCCCCCCCEEEEEC
GPELSEVRVCMGKDLQFGACGKGVKTQCRAGDIRVPPSR
CCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCC
>Mature Secondary Structure
MKKLFTIMALIALTVGSIGLSSARQSSASKAESVAGVFDYYLLTLSWSPTFCLTHKDDPQ
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCC
CTGKGYGFVLHGLWPQYAKGGWPESCPPLTNLSAAETAKGLTLFPTKKLLDHEWSKHGTC
CCCCCCEEEEECCCHHHHCCCCCCCCCCCCCCCHHHHCCCCEECCCHHHHHCCCCCCCCC
SGLGAMGYLDEADKAVGAVKIPQELQPFSSSYYFEAQEIADLFRRSNPGIPSDGIAVICN
CCCCCCCCHHHHHHHHCEEECCHHHCCCCCCCEECHHHHHHHHHHCCCCCCCCCEEEEEC
GPELSEVRVCMGKDLQFGACGKGVKTQCRAGDIRVPPSR
CCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685334 [H]