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The map label for this gene is selU

Identifier: 77459976

GI number: 77459976

Start: 4242244

End: 4243347

Strand: Reverse

Name: selU

Synonym: Pfl01_3754

Alternate gene names: 77459976

Gene position: 4243347-4242244 (Counterclockwise)

Preceding gene: 77459977

Following gene: 77459974

Centisome position: 65.91

GC content: 61.96

Gene sequence:

>1104_bases
ATGTCCATCGACGTCACCGACTACCGCGACATCTTCCTCAACGACCGGCCGCTGATGGACGCCCGTGCGCCGGTCGAGTT
TCACAAGGGCGCGTTTCCAGGGGCGGTCAATCTGCCGCTGATGAATGACATCGAGCGCCAGCGGGTCGGCACTTGCTACA
AGCATCACGGTCAGCAGGCAGCCATTGAGCTGGGTCATCAACTGGTGTCCGGCCCGGTGAAGGAAGAGCGTATCCAGGCC
TGGGCGGAGTTTGCCCGGACGCATCCCGAGGGTTATCTGTATTGCTTTCGTGGCGGCCTGCGTTCGCAAATCGTGCAGCA
GTGGCTGAAGTCGGAAGCCGGCGTCGATTACCCGCGTATCGGCGGTGGCTACAAGGCACTGCGGGGTTTTCTGATCGACA
CTCTTGAATCAGCCTTGACCGACTGTGATTTCGTGCTGCTGGGCGGCATGACCGGCACCGGCAAGACCGAAGTGCTGGCG
CAACTGCGCAACAGCGTGGATCTGGAAGGCCATGCCAATCACCGGGGCTCAAGTTTCGGCAAGCGCGCCACCGGTCAGCC
GTCGAACATCGATTTCGAGAATCGGCTGGCCATCGACTTGCTGAAAAAACGCGCTGCCGGCATCGATCAATTTGTGCTGG
AGGATGAGAGCCGGGTGGTCGGCAGCTGCGCGTTGCCCTTGCCGTTGTATCAGGGCATGCAGCAGTACCCGATGGTCTGG
CTCGAAGATCGTTTCGAGCAGCGCGTCGAGCGGATCCTGCGCGATTACGTGGTGGATTTGTCTGCCGAGTTTCAGGCTGT
GCATGGGGAGGAAGGCTTCGGGCGGTTTTGCGAGCGTTTGCTGGAGAGCCTCGACAACGTGCACAAGCGCCTGGGCGGCG
AGCGGCATCGACGGATGCGGGTGTTGATGGAGGAGGCGCTGGCGGAGCAGGGCCGCAGCGGGGCGGTGGAGTTGCATCGC
GCGTGGATCGAGGGCTTGCTGCGCGAGTATTACGACCCCATGTATGTGTTTCAGCGCGAGAAGAAGGGCGGGCGGATCGA
GTTTGCCGGGGAGCGGCAGGCCGTTATCGAGTACCTGCGTCAACGGGGCAAATCCCAGGCCTGA

Upstream 100 bases:

>100_bases
GCAATGCCGAATTCCTCGCCGTCGCTGCCGAACTCGGCCTCGCGCTTGAGCCGATCGGTGAGTTGATCGGGCGACAGAGC
CACGCGGTCGAGGTGAATTG

Downstream 100 bases:

>100_bases
CGGGGCCCGAACGGGCCCCGGGCACTCTCAGGTCGGACAGCTTTCCTGGCCGTTATCCAGCCCCTGTTTGTAGCTGTGGC
TCTGCAGGCTGGCCTTGCCG

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSIDVTDYRDIFLNDRPLMDARAPVEFHKGAFPGAVNLPLMNDIERQRVGTCYKHHGQQAAIELGHQLVSGPVKEERIQA
WAEFARTHPEGYLYCFRGGLRSQIVQQWLKSEAGVDYPRIGGGYKALRGFLIDTLESALTDCDFVLLGGMTGTGKTEVLA
QLRNSVDLEGHANHRGSSFGKRATGQPSNIDFENRLAIDLLKKRAAGIDQFVLEDESRVVGSCALPLPLYQGMQQYPMVW
LEDRFEQRVERILRDYVVDLSAEFQAVHGEEGFGRFCERLLESLDNVHKRLGGERHRRMRVLMEEALAEQGRSGAVELHR
AWIEGLLREYYDPMYVFQREKKGGRIEFAGERQAVIEYLRQRGKSQA

Sequences:

>Translated_367_residues
MSIDVTDYRDIFLNDRPLMDARAPVEFHKGAFPGAVNLPLMNDIERQRVGTCYKHHGQQAAIELGHQLVSGPVKEERIQA
WAEFARTHPEGYLYCFRGGLRSQIVQQWLKSEAGVDYPRIGGGYKALRGFLIDTLESALTDCDFVLLGGMTGTGKTEVLA
QLRNSVDLEGHANHRGSSFGKRATGQPSNIDFENRLAIDLLKKRAAGIDQFVLEDESRVVGSCALPLPLYQGMQQYPMVW
LEDRFEQRVERILRDYVVDLSAEFQAVHGEEGFGRFCERLLESLDNVHKRLGGERHRRMRVLMEEALAEQGRSGAVELHR
AWIEGLLREYYDPMYVFQREKKGGRIEFAGERQAVIEYLRQRGKSQA
>Mature_366_residues
SIDVTDYRDIFLNDRPLMDARAPVEFHKGAFPGAVNLPLMNDIERQRVGTCYKHHGQQAAIELGHQLVSGPVKEERIQAW
AEFARTHPEGYLYCFRGGLRSQIVQQWLKSEAGVDYPRIGGGYKALRGFLIDTLESALTDCDFVLLGGMTGTGKTEVLAQ
LRNSVDLEGHANHRGSSFGKRATGQPSNIDFENRLAIDLLKKRAAGIDQFVLEDESRVVGSCALPLPLYQGMQQYPMVWL
EDRFEQRVERILRDYVVDLSAEFQAVHGEEGFGRFCERLLESLDNVHKRLGGERHRRMRVLMEEALAEQGRSGAVELHRA
WIEGLLREYYDPMYVFQREKKGGRIEFAGERQAVIEYLRQRGKSQA

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

Organism=Escherichia coli, GI1786712, Length=359, Percent_Identity=45.6824512534819, Blast_Score=306, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SELU_PSEPF (Q3K9R2)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_349483.1
- ProteinModelPortal:   Q3K9R2
- SMR:   Q3K9R2
- STRING:   Q3K9R2
- GeneID:   3714686
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_3754
- eggNOG:   COG2603
- HOGENOM:   HBG452364
- OMA:   NLPLMND
- ProtClustDB:   PRK11784
- BioCyc:   PFLU205922:PFL_3754-MONOMER
- HAMAP:   MF_01622
- InterPro:   IPR001763
- InterPro:   IPR017582
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450
- TIGRFAMs:   TIGR03167

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: NA

Molecular weight: Translated: 41759; Mature: 41628

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 95-95

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDVTDYRDIFLNDRPLMDARAPVEFHKGAFPGAVNLPLMNDIERQRVGTCYKHHGQQA
CCCCCCCHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
AIELGHQLVSGPVKEERIQAWAEFARTHPEGYLYCFRGGLRSQIVQQWLKSEAGVDYPRI
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCC
GGGYKALRGFLIDTLESALTDCDFVLLGGMTGTGKTEVLAQLRNSVDLEGHANHRGSSFG
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
KRATGQPSNIDFENRLAIDLLKKRAAGIDQFVLEDESRVVGSCALPLPLYQGMQQYPMVW
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHCCCCCHHHHCHHHCCHHH
LEDRFEQRVERILRDYVVDLSAEFQAVHGEEGFGRFCERLLESLDNVHKRLGGERHRRMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VLMEEALAEQGRSGAVELHRAWIEGLLREYYDPMYVFQREKKGGRIEFAGERQAVIEYLR
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCCHHHHHHHHH
QRGKSQA
HHCCCCC
>Mature Secondary Structure 
SIDVTDYRDIFLNDRPLMDARAPVEFHKGAFPGAVNLPLMNDIERQRVGTCYKHHGQQA
CCCCCCHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
AIELGHQLVSGPVKEERIQAWAEFARTHPEGYLYCFRGGLRSQIVQQWLKSEAGVDYPRI
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCC
GGGYKALRGFLIDTLESALTDCDFVLLGGMTGTGKTEVLAQLRNSVDLEGHANHRGSSFG
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
KRATGQPSNIDFENRLAIDLLKKRAAGIDQFVLEDESRVVGSCALPLPLYQGMQQYPMVW
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHCCCCCHHHHCHHHCCHHH
LEDRFEQRVERILRDYVVDLSAEFQAVHGEEGFGRFCERLLESLDNVHKRLGGERHRRMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VLMEEALAEQGRSGAVELHRAWIEGLLREYYDPMYVFQREKKGGRIEFAGERQAVIEYLR
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCCHHHHHHHHH
QRGKSQA
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA