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The map label for this gene is ampR [H]

Identifier: 77459944

GI number: 77459944

Start: 4207987

End: 4208862

Strand: Reverse

Name: ampR [H]

Synonym: Pfl01_3722

Alternate gene names: 77459944

Gene position: 4208862-4207987 (Counterclockwise)

Preceding gene: 77459946

Following gene: 77459941

Centisome position: 65.37

GC content: 63.24

Gene sequence:

>876_bases
ATGATTCGACCTCAATTGCCCTTGAATGCGTTACGTGCCTTCGAAGCCTCTGCGCGTCATTTGAGCTTTACTCGCGCCGC
CGTGGAGTTGTGCGTGACCCAGGCGGCCGTCAGCCATCAGGTCAAAAGTCTGGAAGCGCAACTCGGCGTGATCCTGTTCA
AACGCCTGCCCCGTGGGTTGATGCTGACCGGCGAAGGCGAAACCCTGCTGCCGGTGCTGACCACGTCATTCGATCATATC
GCGCAGATGCTCGAGCGTCTGGCGGGCGGGCAATATCGGGAAATGCTCACGGTCGGCGCGGTGGGCACTTTCGCGGTGGG
CTGGTTGTTGCCGAGGCTGGCGGATTTTCAGGCGAAACATCCGCTCATAGATTTGCGGCTGTCGACCAACAACAATCGGG
TGGACGTGGCCGCCGAAGGGCTGGATTACGCGATCCGGTTTGGCGCCGGGGCGTGGCACGGTATCGAGGCGACCCGTTTG
CTGGAGGCGCCGTTGTCGGTGCTTTGCGTGCCGGAAATCGCCCGGCAACTGCACACGCCGGGGGATCTTTTGCAGCAGCG
TTTGCTGCGTTCCTATCGCACCGATGAGTGGCCGGAGTGGTTTCATGCGGCCGGTCTTGCGACCCATGCCGCGCCGCCCC
AGAGCATCGTGTTCGACTCGTCGCTGGCGATGATGGAGGCGGCGTTGCAGGGCGGCGGGGTGGCACTGGCGCCGCCGTTG
ATGTTTGCCCGGCAACTGGCGGCGGATCTGATCCGCCAACCCTTTGCCATTGAAATCACCACCGGCAGCTATTGGCTGAC
GCGCTTGCAGTCACGGCCGGAGACGTCGGCGATGGCGGCGTTCAGGCACTGGTTGCTGGAGGTTGCGCAGGCCTAG

Upstream 100 bases:

>100_bases
CATTGGGCTGCTGATCGGCGCCGCCAAATCTAGGCAGTCCGCTGTCTTTCGACAAACGATGAAAATTCGGACGAGCCATT
AGAAAAATTTGGGACTTGGC

Downstream 100 bases:

>100_bases
CGCACCAGACATGGCTTCTTGTTATCGAACGTCCAGCCCGGAATCAGAAACTGCATCGCCACACTGTCATCCCGCGCGCC
GAGCCCCATGCCTTTATAGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MIRPQLPLNALRAFEASARHLSFTRAAVELCVTQAAVSHQVKSLEAQLGVILFKRLPRGLMLTGEGETLLPVLTTSFDHI
AQMLERLAGGQYREMLTVGAVGTFAVGWLLPRLADFQAKHPLIDLRLSTNNNRVDVAAEGLDYAIRFGAGAWHGIEATRL
LEAPLSVLCVPEIARQLHTPGDLLQQRLLRSYRTDEWPEWFHAAGLATHAAPPQSIVFDSSLAMMEAALQGGGVALAPPL
MFARQLAADLIRQPFAIEITTGSYWLTRLQSRPETSAMAAFRHWLLEVAQA

Sequences:

>Translated_291_residues
MIRPQLPLNALRAFEASARHLSFTRAAVELCVTQAAVSHQVKSLEAQLGVILFKRLPRGLMLTGEGETLLPVLTTSFDHI
AQMLERLAGGQYREMLTVGAVGTFAVGWLLPRLADFQAKHPLIDLRLSTNNNRVDVAAEGLDYAIRFGAGAWHGIEATRL
LEAPLSVLCVPEIARQLHTPGDLLQQRLLRSYRTDEWPEWFHAAGLATHAAPPQSIVFDSSLAMMEAALQGGGVALAPPL
MFARQLAADLIRQPFAIEITTGSYWLTRLQSRPETSAMAAFRHWLLEVAQA
>Mature_291_residues
MIRPQLPLNALRAFEASARHLSFTRAAVELCVTQAAVSHQVKSLEAQLGVILFKRLPRGLMLTGEGETLLPVLTTSFDHI
AQMLERLAGGQYREMLTVGAVGTFAVGWLLPRLADFQAKHPLIDLRLSTNNNRVDVAAEGLDYAIRFGAGAWHGIEATRL
LEAPLSVLCVPEIARQLHTPGDLLQQRLLRSYRTDEWPEWFHAAGLATHAAPPQSIVFDSSLAMMEAALQGGGVALAPPL
MFARQLAADLIRQPFAIEITTGSYWLTRLQSRPETSAMAAFRHWLLEVAQA

Specific function: This protein is a positive regulator of gene expression of beta-lactamase (AmpC) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=38.4083044982699, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=34.2657342657343, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=31.0580204778157, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1787128, Length=242, Percent_Identity=29.7520661157025, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787589, Length=178, Percent_Identity=30.8988764044944, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693193, Length=310, Percent_Identity=27.0967741935484, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693105, Length=169, Percent_Identity=31.3609467455621, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786401, Length=241, Percent_Identity=24.896265560166, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=167, Percent_Identity=32.3353293413174, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081978, Length=179, Percent_Identity=30.1675977653631, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31973; Mature: 31973

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPQLPLNALRAFEASARHLSFTRAAVELCVTQAAVSHQVKSLEAQLGVILFKRLPRGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
MLTGEGETLLPVLTTSFDHIAQMLERLAGGQYREMLTVGAVGTFAVGWLLPRLADFQAKH
EEECCCCEEHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLIDLRLSTNNNRVDVAAEGLDYAIRFGAGAWHGIEATRLLEAPLSVLCVPEIARQLHTP
CEEEEEECCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCHHHEEHHHHHHHHCCC
GDLLQQRLLRSYRTDEWPEWFHAAGLATHAAPPQSIVFDSSLAMMEAALQGGGVALAPPL
HHHHHHHHHHHHCCCCCHHHHHHHCCHHCCCCCCHHEECHHHHHHHHHHCCCCEEECCHH
MFARQLAADLIRQPFAIEITTGSYWLTRLQSRPETSAMAAFRHWLLEVAQA
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIRPQLPLNALRAFEASARHLSFTRAAVELCVTQAAVSHQVKSLEAQLGVILFKRLPRGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
MLTGEGETLLPVLTTSFDHIAQMLERLAGGQYREMLTVGAVGTFAVGWLLPRLADFQAKH
EEECCCCEEHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLIDLRLSTNNNRVDVAAEGLDYAIRFGAGAWHGIEATRLLEAPLSVLCVPEIARQLHTP
CEEEEEECCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCHHHEEHHHHHHHHCCC
GDLLQQRLLRSYRTDEWPEWFHAAGLATHAAPPQSIVFDSSLAMMEAALQGGGVALAPPL
HHHHHHHHHHHHCCCCCHHHHHHHCCHHCCCCCCHHEECHHHHHHHHHHCCCCEEECCHH
MFARQLAADLIRQPFAIEITTGSYWLTRLQSRPETSAMAAFRHWLLEVAQA
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8405939; 10984043; 2125210 [H]