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The map label for this gene is gyaR [H]

Identifier: 77459622

GI number: 77459622

Start: 3876988

End: 3877956

Strand: Direct

Name: gyaR [H]

Synonym: Pfl01_3400

Alternate gene names: 77459622

Gene position: 3876988-3877956 (Clockwise)

Preceding gene: 77459621

Following gene: 77459628

Centisome position: 60.22

GC content: 65.22

Gene sequence:

>969_bases
ATGCCCGCCACCGTTCTGGTACTGGTTGAAACCATCAATGACTATTTGCCGATCCTCGAACGTCAGGGTTTTCACCTGAT
TCTCGCGCCGACCCCAGCCGAACGCGCGCAAGCCATCGCCACCCACGGGGCGCGGATCGACGCGGTACTGACCCGTGGCC
CGCTGGGCTTATATGCTGAAGAAATCGCGGCCTTGCCCGCGCTGAAAATCATCTGCGTGATCGGCGCCGGTTACGAGCAG
GTCGACCTGCAGGCCGCCAGTGACCGTGGGCTGACCGTGACCAACGGCGCCGGCGTCAACGCTTCGTCGGTGGCCGACCA
CGCCATGGCGATGCTGCTGGCGCTGGTGCGCGACATCCCGCGCTGCGATGGGGCGGTGCGTCGGGGCGAGTGGCCGAAGA
TCATGCGCCCGTCGCTGGCCGGCAAGCGCCTGGGCATTCTCGGTCTGGGCGCGGTGGGGATGGCGATTGCCAAACGCGCG
GGCCTGGGCTTCGACATGGAGATCAGCTACCACAACCGTCAGGTGCGCAGCGACGTGCCTTACGCGTTCTGCTCGACCCC
GACCGAACTGGCCCGGGCCTCGGATTTTCTGGTAGTCGCCACGCCCGGCGGCATCGGCAGCCAGCATCTGGTGACCCGCC
CGGTGCTCGATGCTTTGGGTCCCAACGGTTTTATCGTCAACATTGCCCGCGCCAGCGTGATCGCCACCGCCGATCTGATC
AGCGCGCTGGAACAACGGCGAATTGCCGGCGCAGCGCTGGATGTGTTCGATCACGAGCCGCAAGTACCCGATGCGCTGAA
GAGCCTGAGCAATGTGCTTCTCACCCCGCACGTCGCCGGGCTGTCACCGGAAGCCACTCAAGGCACCGTGGAGCTGGTGG
GCAAGAATCTGGTGGCGTTCTTCTCCGGGCAACCGGTGCTGACGCCGCTGCAGTTGCCGCCGAAACTGAACAACCAGCGC
GTGCACTAA

Upstream 100 bases:

>100_bases
GCCTGATGCGGGGCCGCGCCAGCCATTGCCAAACCGCTCTGGAATCAGCACTCTGAACCGACTTTTGCGCCCGAAGACCG
ACGATCACAGGAGCCGAGCA

Downstream 100 bases:

>100_bases
AGCACGCCGAGCAAAGTCCACAGACGCCGGGATTCGGCGTCTGCCGAAATCAGCTCGCCGAGCAGCTCGCTTAACGGCTT
GTTGCCCCATTCCACCCCGC

Product: 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MPATVLVLVETINDYLPILERQGFHLILAPTPAERAQAIATHGARIDAVLTRGPLGLYAEEIAALPALKIICVIGAGYEQ
VDLQAASDRGLTVTNGAGVNASSVADHAMAMLLALVRDIPRCDGAVRRGEWPKIMRPSLAGKRLGILGLGAVGMAIAKRA
GLGFDMEISYHNRQVRSDVPYAFCSTPTELARASDFLVVATPGGIGSQHLVTRPVLDALGPNGFIVNIARASVIATADLI
SALEQRRIAGAALDVFDHEPQVPDALKSLSNVLLTPHVAGLSPEATQGTVELVGKNLVAFFSGQPVLTPLQLPPKLNNQR
VH

Sequences:

>Translated_322_residues
MPATVLVLVETINDYLPILERQGFHLILAPTPAERAQAIATHGARIDAVLTRGPLGLYAEEIAALPALKIICVIGAGYEQ
VDLQAASDRGLTVTNGAGVNASSVADHAMAMLLALVRDIPRCDGAVRRGEWPKIMRPSLAGKRLGILGLGAVGMAIAKRA
GLGFDMEISYHNRQVRSDVPYAFCSTPTELARASDFLVVATPGGIGSQHLVTRPVLDALGPNGFIVNIARASVIATADLI
SALEQRRIAGAALDVFDHEPQVPDALKSLSNVLLTPHVAGLSPEATQGTVELVGKNLVAFFSGQPVLTPLQLPPKLNNQR
VH
>Mature_321_residues
PATVLVLVETINDYLPILERQGFHLILAPTPAERAQAIATHGARIDAVLTRGPLGLYAEEIAALPALKIICVIGAGYEQV
DLQAASDRGLTVTNGAGVNASSVADHAMAMLLALVRDIPRCDGAVRRGEWPKIMRPSLAGKRLGILGLGAVGMAIAKRAG
LGFDMEISYHNRQVRSDVPYAFCSTPTELARASDFLVVATPGGIGSQHLVTRPVLDALGPNGFIVNIARASVIATADLIS
ALEQRRIAGAALDVFDHEPQVPDALKSLSNVLLTPHVAGLSPEATQGTVELVGKNLVAFFSGQPVLTPLQLPPKLNNQRV
H

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=265, Percent_Identity=29.811320754717, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI6912396, Length=256, Percent_Identity=31.640625, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI61743967, Length=279, Percent_Identity=28.673835125448, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI4557497, Length=247, Percent_Identity=30.3643724696356, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI145580578, Length=249, Percent_Identity=28.1124497991968, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI4557499, Length=249, Percent_Identity=28.1124497991968, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI145580575, Length=247, Percent_Identity=28.3400809716599, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI87082289, Length=253, Percent_Identity=30.0395256916996, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789279, Length=261, Percent_Identity=29.8850574712644, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787645, Length=263, Percent_Identity=26.615969581749, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=266, Percent_Identity=25.9398496240602, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25147481, Length=251, Percent_Identity=27.8884462151394, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324055, Length=278, Percent_Identity=32.3741007194245, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=312, Percent_Identity=27.5641025641026, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322116, Length=312, Percent_Identity=26.6025641025641, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324964, Length=325, Percent_Identity=24.6153846153846, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321253, Length=172, Percent_Identity=28.4883720930233, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45551003, Length=275, Percent_Identity=29.8181818181818, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24585514, Length=275, Percent_Identity=29.8181818181818, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28574282, Length=275, Percent_Identity=29.8181818181818, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28574284, Length=275, Percent_Identity=29.8181818181818, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI45552429, Length=275, Percent_Identity=29.8181818181818, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28574286, Length=259, Percent_Identity=28.957528957529, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24646446, Length=240, Percent_Identity=31.6666666666667, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24646448, Length=240, Percent_Identity=31.6666666666667, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24646452, Length=240, Percent_Identity=31.6666666666667, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24646450, Length=240, Percent_Identity=31.6666666666667, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI62472511, Length=240, Percent_Identity=31.6666666666667, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28571528, Length=259, Percent_Identity=28.5714285714286, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19921140, Length=313, Percent_Identity=26.517571884984, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24585516, Length=256, Percent_Identity=26.171875, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 33946; Mature: 33815

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATVLVLVETINDYLPILERQGFHLILAPTPAERAQAIATHGARIDAVLTRGPLGLYAE
CCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEEEEECCCCHHHHH
EIAALPALKIICVIGAGYEQVDLQAASDRGLTVTNGAGVNASSVADHAMAMLLALVRDIP
HHHHHHHEEEEEEECCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
RCDGAVRRGEWPKIMRPSLAGKRLGILGLGAVGMAIAKRAGLGFDMEISYHNRQVRSDVP
CCCCCCCCCCCCHHCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCC
YAFCSTPTELARASDFLVVATPGGIGSQHLVTRPVLDALGPNGFIVNIARASVIATADLI
EEEECCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHH
SALEQRRIAGAALDVFDHEPQVPDALKSLSNVLLTPHVAGLSPEATQGTVELVGKNLVAF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCEEEE
FSGQPVLTPLQLPPKLNNQRVH
ECCCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure 
PATVLVLVETINDYLPILERQGFHLILAPTPAERAQAIATHGARIDAVLTRGPLGLYAE
CCEEEEHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEEEEECCCCHHHHH
EIAALPALKIICVIGAGYEQVDLQAASDRGLTVTNGAGVNASSVADHAMAMLLALVRDIP
HHHHHHHEEEEEEECCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
RCDGAVRRGEWPKIMRPSLAGKRLGILGLGAVGMAIAKRAGLGFDMEISYHNRQVRSDVP
CCCCCCCCCCCCHHCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCC
YAFCSTPTELARASDFLVVATPGGIGSQHLVTRPVLDALGPNGFIVNIARASVIATADLI
EEEECCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHH
SALEQRRIAGAALDVFDHEPQVPDALKSLSNVLLTPHVAGLSPEATQGTVELVGKNLVAF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCEEEE
FSGQPVLTPLQLPPKLNNQRVH
ECCCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA