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The map label for this gene is miaE [H]

Identifier: 77459614

GI number: 77459614

Start: 3868833

End: 3869441

Strand: Reverse

Name: miaE [H]

Synonym: Pfl01_3392

Alternate gene names: 77459614

Gene position: 3869441-3868833 (Counterclockwise)

Preceding gene: 77459616

Following gene: 77459613

Centisome position: 60.1

GC content: 60.92

Gene sequence:

>609_bases
ATGATCCTTCCCGAAATCCACGAATTCCTTGGTTGCCGCACGCCTGACGCCTGGGTCCAGGCCGCGCTGGCCGATCAGGA
AACCCTGCTGATCGACCACAAGAACTGCGAGTTCAAGGCCGCCAGCACCGCGTTGAGCCTGATCGCCAAGTACCATTCCC
ACGTCGATCTGATCAACATGATGTCGCGTCTGGCCCGGGAAGAACTGGTGCACCACGAGCAGGTGATGCGCATAATGAAG
CGGCGCAAGATCGAACTGCGCCAGCTCCACGCCGGCCGTTATGCCTCGAGCCTGCGCAAAGTGGTGCGCAGCCATGAACC
GGTGAAACTGGTCGACACCCTGGTGGTCGGCGCCTTTATCGAAGCGCGCAGTTGCGAGCGGTTCGAAGCGCTGGTGCCGC
ATCTGGACGAAGAACTCGGCAAGTTCTACTTCGGCCTGCTGAAAAGCGAGGCCCGGCATTTCCAGGGTTACCTGAAACTG
GCCTACCAGTACGGTGACGCCAAGGACATCGCCCAGGTGATCGAGAGAGTCCGCGCCGCCGAGCAGGACCTGATCGAGTC
GCCGGACGAGGAATTCCGTTTCCACAGTGGTGTACCCGTCGCGGCATGA

Upstream 100 bases:

>100_bases
GTCTGCGACTTGATGCATATCAACGCGCGCGGCTGGTGGTCTGCGGGCAGGCCGGTATAATCGGCGCCCTTCGCTCGAAC
ACCTTTTGACCGTGAGCCCC

Downstream 100 bases:

>100_bases
ATTGTTAAAAACTCTTAAGAGTATGAAACATCAACGAAAACCGGCCCCGGAGGCCGGTTTTTGCTGCCTGCGATAAACGC
CTCGCCAGTGAATGCCCGCA

Product: tRNA--hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MILPEIHEFLGCRTPDAWVQAALADQETLLIDHKNCEFKAASTALSLIAKYHSHVDLINMMSRLAREELVHHEQVMRIMK
RRKIELRQLHAGRYASSLRKVVRSHEPVKLVDTLVVGAFIEARSCERFEALVPHLDEELGKFYFGLLKSEARHFQGYLKL
AYQYGDAKDIAQVIERVRAAEQDLIESPDEEFRFHSGVPVAA

Sequences:

>Translated_202_residues
MILPEIHEFLGCRTPDAWVQAALADQETLLIDHKNCEFKAASTALSLIAKYHSHVDLINMMSRLAREELVHHEQVMRIMK
RRKIELRQLHAGRYASSLRKVVRSHEPVKLVDTLVVGAFIEARSCERFEALVPHLDEELGKFYFGLLKSEARHFQGYLKL
AYQYGDAKDIAQVIERVRAAEQDLIESPDEEFRFHSGVPVAA
>Mature_202_residues
MILPEIHEFLGCRTPDAWVQAALADQETLLIDHKNCEFKAASTALSLIAKYHSHVDLINMMSRLAREELVHHEQVMRIMK
RRKIELRQLHAGRYASSLRKVVRSHEPVKLVDTLVVGAFIEARSCERFEALVPHLDEELGKFYFGLLKSEARHFQGYLKL
AYQYGDAKDIAQVIERVRAAEQDLIESPDEEFRFHSGVPVAA

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 23177; Mature: 23177

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPEIHEFLGCRTPDAWVQAALADQETLLIDHKNCEFKAASTALSLIAKYHSHVDLINM
CCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
MSRLAREELVHHEQVMRIMKRRKIELRQLHAGRYASSLRKVVRSHEPVKLVDTLVVGAFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EARSCERFEALVPHLDEELGKFYFGLLKSEARHFQGYLKLAYQYGDAKDIAQVIERVRAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EQDLIESPDEEFRFHSGVPVAA
HHHHHHCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MILPEIHEFLGCRTPDAWVQAALADQETLLIDHKNCEFKAASTALSLIAKYHSHVDLINM
CCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
MSRLAREELVHHEQVMRIMKRRKIELRQLHAGRYASSLRKVVRSHEPVKLVDTLVVGAFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EARSCERFEALVPHLDEELGKFYFGLLKSEARHFQGYLKLAYQYGDAKDIAQVIERVRAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EQDLIESPDEEFRFHSGVPVAA
HHHHHHCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]