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The map label for this gene is rstB [H]

Identifier: 77459612

GI number: 77459612

Start: 3866698

End: 3867984

Strand: Reverse

Name: rstB [H]

Synonym: Pfl01_3390

Alternate gene names: 77459612

Gene position: 3867984-3866698 (Counterclockwise)

Preceding gene: 77459613

Following gene: 77459609

Centisome position: 60.08

GC content: 64.18

Gene sequence:

>1287_bases
ATGCTGCGGTTATTTCTCGGGCTGTTTCTGGTGATGACCGTCGGGCTGGTGGCGGCGCTGCAAACCGTCGAACACACCTT
CAACGCGTTGCTCGACAACCAGATGCAGGCCTACAACCGCGAGGCGGTGCGCGGTCAGGCCTGGTCGCTGATCGAACACA
TGCGCGACCAGCAGGGCGCGGCGCGCGAGGCCCAACTGGAGGCGTTGCGGCCGCACTACGGGTTGACGCTGAGCCTGGTC
GAGGCTGACCAATTGAACCTCAACGATCAGGAAAAAGCCGAACTGGCCCAGGATCTGCTGGTGATTCGCGATGACTACAC
CCAGTTCATCAGCAACATCGACGGTGGTTCGCAGTTGCTCAGCATCAAATTGCCGCCCGAGCCGAGCCTGATGCCGTTCT
ACATTGCCGGGGCCTACCTGATGCTGGCCGTGTTGCTCGGCATCGTCCTGTACTTCTGGGTGCGCCCGCACTGGCGCGAT
CTGGAAAAACTGCGGCTGGCCGCCGAGCGTTTCGGTGACAACGACCTTTCGGTGCGGATCCAGCTGTCCAAGCGCTCGAA
CATCCGCGACCTGGCCGGCCACTTCAACCTGATGGCGGCGCGCATCGAAGGCCTGATCGCCAATCAGCGCGAACTGACCA
ACGCGGTCTCCCACGAACTGCGCACGCCGATTGCCCGGTTGTCGTTCGAGCTCGATCAGCTCAAGCAGCAACCGGACGCC
AGCCAGAACCGCGAACTGATCGCCGACATGTACGCCGACCTCGGCGAGCTGGAAGAAATGGTCTCCGAACTGCTGACCTA
CGCCAGCCTCGAGCGCGGCGCGACGGTGATCACCCGCGAAAACATTCAGGCTGCCAACTGGCTCGACAGCGTGGTCGGCA
GCGTGGCGCTGGAAGCCGAGGCGGCCGGGGTGCAGCTGTTGATCGTAGATTGCCGGGTCGACGAAGTGCGGATCGAACCG
CGTTTCATGGCGCGTGCGGTGATCAATCTGCTGCGCAATGCCATCCGGTATGCCGAGCAGCGGGTCGAGGTGTCGCTGGT
GCGCGTTGGCGATCAATACGAAGTGCAGGTCAACGACGACGGGCCGGGTGTGCCGCTGGCAGGGCGGGAGAAAATCTTCG
AACCGTTCTCGCGTCTGGACGCCAGCCGTGACCGCCGCACGGGCGGCTTCGGCCTCGGCCTGGCGCTGGTGCGGCGGGTG
TCGCAATCCCATGGCGGGCAGGTCGAGGTCGGCGACTCGCCGTGGGGCGGCGCTTCGTTCCGCATGACCTGGGCGCATCT
GGATTGA

Upstream 100 bases:

>100_bases
ATTGCGCAACAAACTCAAGGACAACCCTCGCGAACCGGTGTGCATCAAGACCGTGTGGGGCAAGGGTTACCTGTTCAATC
CGTTTGCGTGGGAGTTGTAG

Downstream 100 bases:

>100_bases
TGAGTCTCCCTCCCCGGAAGGGAAGGCAGTCGATTAACTGAGGACGTAGTCCACCGGTTTCAGCGCTGGCGGCAACGGTT
CAGTCCCCAGCTCCGAGAGA

Product: periplasmic sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MLRLFLGLFLVMTVGLVAALQTVEHTFNALLDNQMQAYNREAVRGQAWSLIEHMRDQQGAAREAQLEALRPHYGLTLSLV
EADQLNLNDQEKAELAQDLLVIRDDYTQFISNIDGGSQLLSIKLPPEPSLMPFYIAGAYLMLAVLLGIVLYFWVRPHWRD
LEKLRLAAERFGDNDLSVRIQLSKRSNIRDLAGHFNLMAARIEGLIANQRELTNAVSHELRTPIARLSFELDQLKQQPDA
SQNRELIADMYADLGELEEMVSELLTYASLERGATVITRENIQAANWLDSVVGSVALEAEAAGVQLLIVDCRVDEVRIEP
RFMARAVINLLRNAIRYAEQRVEVSLVRVGDQYEVQVNDDGPGVPLAGREKIFEPFSRLDASRDRRTGGFGLGLALVRRV
SQSHGGQVEVGDSPWGGASFRMTWAHLD

Sequences:

>Translated_428_residues
MLRLFLGLFLVMTVGLVAALQTVEHTFNALLDNQMQAYNREAVRGQAWSLIEHMRDQQGAAREAQLEALRPHYGLTLSLV
EADQLNLNDQEKAELAQDLLVIRDDYTQFISNIDGGSQLLSIKLPPEPSLMPFYIAGAYLMLAVLLGIVLYFWVRPHWRD
LEKLRLAAERFGDNDLSVRIQLSKRSNIRDLAGHFNLMAARIEGLIANQRELTNAVSHELRTPIARLSFELDQLKQQPDA
SQNRELIADMYADLGELEEMVSELLTYASLERGATVITRENIQAANWLDSVVGSVALEAEAAGVQLLIVDCRVDEVRIEP
RFMARAVINLLRNAIRYAEQRVEVSLVRVGDQYEVQVNDDGPGVPLAGREKIFEPFSRLDASRDRRTGGFGLGLALVRRV
SQSHGGQVEVGDSPWGGASFRMTWAHLD
>Mature_428_residues
MLRLFLGLFLVMTVGLVAALQTVEHTFNALLDNQMQAYNREAVRGQAWSLIEHMRDQQGAAREAQLEALRPHYGLTLSLV
EADQLNLNDQEKAELAQDLLVIRDDYTQFISNIDGGSQLLSIKLPPEPSLMPFYIAGAYLMLAVLLGIVLYFWVRPHWRD
LEKLRLAAERFGDNDLSVRIQLSKRSNIRDLAGHFNLMAARIEGLIANQRELTNAVSHELRTPIARLSFELDQLKQQPDA
SQNRELIADMYADLGELEEMVSELLTYASLERGATVITRENIQAANWLDSVVGSVALEAEAAGVQLLIVDCRVDEVRIEP
RFMARAVINLLRNAIRYAEQRVEVSLVRVGDQYEVQVNDDGPGVPLAGREKIFEPFSRLDASRDRRTGGFGLGLALVRRV
SQSHGGQVEVGDSPWGGASFRMTWAHLD

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=433, Percent_Identity=33.256351039261, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1790346, Length=257, Percent_Identity=33.852140077821, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789808, Length=287, Percent_Identity=30.3135888501742, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786783, Length=290, Percent_Identity=28.6206896551724, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788393, Length=261, Percent_Identity=26.4367816091954, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789149, Length=248, Percent_Identity=29.0322580645161, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786912, Length=240, Percent_Identity=29.1666666666667, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787374, Length=284, Percent_Identity=27.4647887323944, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789403, Length=241, Percent_Identity=27.3858921161826, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786600, Length=237, Percent_Identity=28.2700421940928, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081816, Length=328, Percent_Identity=25.3048780487805, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790551, Length=280, Percent_Identity=27.8571428571429, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87082128, Length=259, Percent_Identity=23.9382239382239, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788549, Length=220, Percent_Identity=24.5454545454545, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790861, Length=289, Percent_Identity=28.719723183391, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693157, Length=215, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788713, Length=231, Percent_Identity=23.3766233766234, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788279, Length=266, Percent_Identity=22.5563909774436, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790436, Length=216, Percent_Identity=23.6111111111111, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47985; Mature: 47985

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLFLGLFLVMTVGLVAALQTVEHTFNALLDNQMQAYNREAVRGQAWSLIEHMRDQQGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AREAQLEALRPHYGLTLSLVEADQLNLNDQEKAELAQDLLVIRDDYTQFISNIDGGSQLL
HHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
SIKLPPEPSLMPFYIAGAYLMLAVLLGIVLYFWVRPHWRDLEKLRLAAERFGDNDLSVRI
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEE
QLSKRSNIRDLAGHFNLMAARIEGLIANQRELTNAVSHELRTPIARLSFELDQLKQQPDA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
SQNRELIADMYADLGELEEMVSELLTYASLERGATVITRENIQAANWLDSVVGSVALEAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHH
AAGVQLLIVDCRVDEVRIEPRFMARAVINLLRNAIRYAEQRVEVSLVRVGDQYEVQVNDD
CCCEEEEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEECCC
GPGVPLAGREKIFEPFSRLDASRDRRTGGFGLGLALVRRVSQSHGGQVEVGDSPWGGASF
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCE
RMTWAHLD
EEEEEECC
>Mature Secondary Structure
MLRLFLGLFLVMTVGLVAALQTVEHTFNALLDNQMQAYNREAVRGQAWSLIEHMRDQQGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AREAQLEALRPHYGLTLSLVEADQLNLNDQEKAELAQDLLVIRDDYTQFISNIDGGSQLL
HHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
SIKLPPEPSLMPFYIAGAYLMLAVLLGIVLYFWVRPHWRDLEKLRLAAERFGDNDLSVRI
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEE
QLSKRSNIRDLAGHFNLMAARIEGLIANQRELTNAVSHELRTPIARLSFELDQLKQQPDA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
SQNRELIADMYADLGELEEMVSELLTYASLERGATVITRENIQAANWLDSVVGSVALEAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHH
AAGVQLLIVDCRVDEVRIEPRFMARAVINLLRNAIRYAEQRVEVSLVRVGDQYEVQVNDD
CCCEEEEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEECCC
GPGVPLAGREKIFEPFSRLDASRDRRTGGFGLGLALVRRVSQSHGGQVEVGDSPWGGASF
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCE
RMTWAHLD
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]