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The map label for this gene is rpfC [H]

Identifier: 77459475

GI number: 77459475

Start: 3733392

End: 3735029

Strand: Reverse

Name: rpfC [H]

Synonym: Pfl01_3253

Alternate gene names: 77459475

Gene position: 3735029-3733392 (Counterclockwise)

Preceding gene: 77459477

Following gene: 77459468

Centisome position: 58.01

GC content: 62.33

Gene sequence:

>1638_bases
ATGAAGTTCGAAAAGAACACCGAGCTCGACCAGGCCAATCTGCGCATCATCATCGCCAGCATTGCGCTGGTTTACATGGC
CGTGCTCGGTTTCCTGCCGGGCCAGCGCTTCGATACTTATCTGCCGGTGGTCACCTACATTTCCCTGTTTCTGCTGGCCT
CCGTTGTGCTTCGTCAAGTCATCGTGCGCTGGCCTGGGCATTATCCGGCGCGGCGGATTTTCGGCATGCTCCACGATTAC
ACCGGCACCAGTTTCGGGCTGGTGGTCGGCGGCGAGGCGGCGTTGCCCATCTATGCCGTCATGGTCTGGGTCAATCTCGG
CAACGGCATGCGCTACGGCTCGCGCTACCTGGCGATCGCCACGGTGCTGGCATTGCTGGCGCTGTTGGCGGTCTATCGAT
TGACGCCGTACTGGCAGGCGCAACCGTTCATGGTGCTGATGCTGATGATCACCAGCACGGTGATTCCGGTGTATGCGCAC
CTGTTGCTGGAGCGTACGCGCAAAGCATCGGAAGAAGCGGTCGCTGCGAACCTTGAGAAGTCGCGCTTCCTGGCCCAGGC
CAGTCATGACCTGCGTCAGCCGATCCACTCCATCGGCCTGTTCACCGCGTGTCTGCGCGAGTCACGACTGGGCGAGGAGG
AGCGGCGGCTGGTGGACAGCATTGACCGTTCGCTGCTCAACGTGTCGCAGCTGTTTCGCTCGATACTCGATCTCTACACC
CTCGACAACGGGCGGATTCTGCCCCGGTTGCAGACGCTTGAGCTGGGTGAATGGCTGGCGGATCTGATCCGCCAGAACGC
CGAAGCCGCACGCTGGGCGGGTGTCGAACTGCGCTTGCGGCCTTGCGAACACTGGGTGCGCACTGATCCGGCGTTGCTCG
CGACGATGGTGCAGAACGTGCTTTCCAATTGCTTCAAATACGGCGCGCACCGGCCGGTGCTGATCGGTGTTCGCAAGCGC
GGCGCGGGGTTGGCCATCGCGATCTATGACCGCGGCAGTGGTATCGCCGAAGAGCATTTGCCCAAGGTGTTCGAGGAGTT
TTATCGGGTGCGCCAGTTGCGCGACAAGGACGTTGAGGGCGTGGGGCTGGGACTGTCGATTGTCCGGCGTCTGGGGCAGT
TGATCGGTGTCGACGTTGCGATCCGCTCACGGCTGGGGCATGGCACGGCCGTGACCTTGTATGGGCTGCCACTCGCATCG
CCGCAGGTGCCGGTCAATCGTGAAGAGGCTCGACAGGTCGGGTTGCTGACAGGGCTGAAGGTGTGTCTGGTGGAAGATGA
TCGTAACGTTCTGTTGGCGACGTCGGCGTTGCTCGAGCGTTGGGGTTGCCTTGTGCAGGCCGAACTCAGCGGACAGGGCC
TGGTCACGGACTGCGACATCATCGTCGCCGACTATGACCTCGGCTCTCACAGCACCGGTATCGAGTGCATCGACGAGGTT
CGTCGTCAGCGCGGTTATGCGGTGCCGGCCATGATCATCACCGGGCACGACATCGAAAAAATCCAGGCGGCGCTGCATGA
CCGCCAGATCGCCATTCTGTCCAAGCCGGTACGCCCGGCCGAGCTGCGCGCGACCCTGCGTGCGCTGCGCGACCGGCAGA
CCGAAGCGGTCAGCGCTTACACAGATAGTCCTGCGTGA

Upstream 100 bases:

>100_bases
TGGGTCTGCATTGCTCATGGCATGTACCTTAGCATCGGGCGAAGGCATTGCCTGTTGATTCGTGCGTTTATGACGACTGC
TGCACAAGGACGTTCCCCCC

Downstream 100 bases:

>100_bases
TGGTGGTTTGCAGGCAGTTGTACTGGCCCATGGTGCGGCAGATGTTTTTCTCCGAGCCCGACACTTCGGCGCTCTTGTAG
CCCCAGACCTTGCAACGTCC

Product: periplasmic sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MKFEKNTELDQANLRIIIASIALVYMAVLGFLPGQRFDTYLPVVTYISLFLLASVVLRQVIVRWPGHYPARRIFGMLHDY
TGTSFGLVVGGEAALPIYAVMVWVNLGNGMRYGSRYLAIATVLALLALLAVYRLTPYWQAQPFMVLMLMITSTVIPVYAH
LLLERTRKASEEAVAANLEKSRFLAQASHDLRQPIHSIGLFTACLRESRLGEEERRLVDSIDRSLLNVSQLFRSILDLYT
LDNGRILPRLQTLELGEWLADLIRQNAEAARWAGVELRLRPCEHWVRTDPALLATMVQNVLSNCFKYGAHRPVLIGVRKR
GAGLAIAIYDRGSGIAEEHLPKVFEEFYRVRQLRDKDVEGVGLGLSIVRRLGQLIGVDVAIRSRLGHGTAVTLYGLPLAS
PQVPVNREEARQVGLLTGLKVCLVEDDRNVLLATSALLERWGCLVQAELSGQGLVTDCDIIVADYDLGSHSTGIECIDEV
RRQRGYAVPAMIITGHDIEKIQAALHDRQIAILSKPVRPAELRATLRALRDRQTEAVSAYTDSPA

Sequences:

>Translated_545_residues
MKFEKNTELDQANLRIIIASIALVYMAVLGFLPGQRFDTYLPVVTYISLFLLASVVLRQVIVRWPGHYPARRIFGMLHDY
TGTSFGLVVGGEAALPIYAVMVWVNLGNGMRYGSRYLAIATVLALLALLAVYRLTPYWQAQPFMVLMLMITSTVIPVYAH
LLLERTRKASEEAVAANLEKSRFLAQASHDLRQPIHSIGLFTACLRESRLGEEERRLVDSIDRSLLNVSQLFRSILDLYT
LDNGRILPRLQTLELGEWLADLIRQNAEAARWAGVELRLRPCEHWVRTDPALLATMVQNVLSNCFKYGAHRPVLIGVRKR
GAGLAIAIYDRGSGIAEEHLPKVFEEFYRVRQLRDKDVEGVGLGLSIVRRLGQLIGVDVAIRSRLGHGTAVTLYGLPLAS
PQVPVNREEARQVGLLTGLKVCLVEDDRNVLLATSALLERWGCLVQAELSGQGLVTDCDIIVADYDLGSHSTGIECIDEV
RRQRGYAVPAMIITGHDIEKIQAALHDRQIAILSKPVRPAELRATLRALRDRQTEAVSAYTDSPA
>Mature_545_residues
MKFEKNTELDQANLRIIIASIALVYMAVLGFLPGQRFDTYLPVVTYISLFLLASVVLRQVIVRWPGHYPARRIFGMLHDY
TGTSFGLVVGGEAALPIYAVMVWVNLGNGMRYGSRYLAIATVLALLALLAVYRLTPYWQAQPFMVLMLMITSTVIPVYAH
LLLERTRKASEEAVAANLEKSRFLAQASHDLRQPIHSIGLFTACLRESRLGEEERRLVDSIDRSLLNVSQLFRSILDLYT
LDNGRILPRLQTLELGEWLADLIRQNAEAARWAGVELRLRPCEHWVRTDPALLATMVQNVLSNCFKYGAHRPVLIGVRKR
GAGLAIAIYDRGSGIAEEHLPKVFEEFYRVRQLRDKDVEGVGLGLSIVRRLGQLIGVDVAIRSRLGHGTAVTLYGLPLAS
PQVPVNREEARQVGLLTGLKVCLVEDDRNVLLATSALLERWGCLVQAELSGQGLVTDCDIIVADYDLGSHSTGIECIDEV
RRQRGYAVPAMIITGHDIEKIQAALHDRQIAILSKPVRPAELRATLRALRDRQTEAVSAYTDSPA

Specific function: Involved in the positive regulation of synthesis of extracellular enzymes and polysaccharide, and hence in pathogenicity. Can restore function to the non-pathogenic mutant 8237 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=391, Percent_Identity=27.6214833759591, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI145693157, Length=396, Percent_Identity=24.7474747474747, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786783, Length=194, Percent_Identity=28.8659793814433, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=26.6949152542373, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788713, Length=271, Percent_Identity=27.6752767527675, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081816, Length=370, Percent_Identity=26.2162162162162, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790346, Length=193, Percent_Identity=27.979274611399, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI87082128, Length=213, Percent_Identity=28.169014084507, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786912, Length=245, Percent_Identity=29.3877551020408, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790436, Length=244, Percent_Identity=24.1803278688525, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60702; Mature: 60702

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFEKNTELDQANLRIIIASIALVYMAVLGFLPGQRFDTYLPVVTYISLFLLASVVLRQV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IVRWPGHYPARRIFGMLHDYTGTSFGLVVGGEAALPIYAVMVWVNLGNGMRYGSRYLAIA
HHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
TVLALLALLAVYRLTPYWQAQPFMVLMLMITSTVIPVYAHLLLERTRKASEEAVAANLEK
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRFLAQASHDLRQPIHSIGLFTACLRESRLGEEERRLVDSIDRSLLNVSQLFRSILDLYT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDNGRILPRLQTLELGEWLADLIRQNAEAARWAGVELRLRPCEHWVRTDPALLATMVQNV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEHHHHHHCCCHHHHHHHHHHH
LSNCFKYGAHRPVLIGVRKRGAGLAIAIYDRGSGIAEEHLPKVFEEFYRVRQLRDKDVEG
HHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VGLGLSIVRRLGQLIGVDVAIRSRLGHGTAVTLYGLPLASPQVPVNREEARQVGLLTGLK
CCHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCE
VCLVEDDRNVLLATSALLERWGCLVQAELSGQGLVTDCDIIVADYDLGSHSTGIECIDEV
EEEEECCCCEEHHHHHHHHHCCCEEEEECCCCCCEEECEEEEEECCCCCCCCCHHHHHHH
RRQRGYAVPAMIITGHDIEKIQAALHDRQIAILSKPVRPAELRATLRALRDRQTEAVSAY
HHHCCCCCCEEEEECCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
TDSPA
CCCCC
>Mature Secondary Structure
MKFEKNTELDQANLRIIIASIALVYMAVLGFLPGQRFDTYLPVVTYISLFLLASVVLRQV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IVRWPGHYPARRIFGMLHDYTGTSFGLVVGGEAALPIYAVMVWVNLGNGMRYGSRYLAIA
HHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
TVLALLALLAVYRLTPYWQAQPFMVLMLMITSTVIPVYAHLLLERTRKASEEAVAANLEK
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRFLAQASHDLRQPIHSIGLFTACLRESRLGEEERRLVDSIDRSLLNVSQLFRSILDLYT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDNGRILPRLQTLELGEWLADLIRQNAEAARWAGVELRLRPCEHWVRTDPALLATMVQNV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEHHHHHHCCCHHHHHHHHHHH
LSNCFKYGAHRPVLIGVRKRGAGLAIAIYDRGSGIAEEHLPKVFEEFYRVRQLRDKDVEG
HHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VGLGLSIVRRLGQLIGVDVAIRSRLGHGTAVTLYGLPLASPQVPVNREEARQVGLLTGLK
CCHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCE
VCLVEDDRNVLLATSALLERWGCLVQAELSGQGLVTDCDIIVADYDLGSHSTGIECIDEV
EEEEECCCCEEHHHHHHHHHCCCEEEEECCCCCCEEECEEEEEECCCCCCCCCHHHHHHH
RRQRGYAVPAMIITGHDIEKIQAALHDRQIAILSKPVRPAELRATLRALRDRQTEAVSAY
HHHCCCCCCEEEEECCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
TDSPA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1645442 [H]