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The map label for this gene is eptA [H]

Identifier: 77459378

GI number: 77459378

Start: 3624528

End: 3626186

Strand: Direct

Name: eptA [H]

Synonym: Pfl01_3156

Alternate gene names: 77459378

Gene position: 3624528-3626186 (Clockwise)

Preceding gene: 77459377

Following gene: 77459390

Centisome position: 56.3

GC content: 60.58

Gene sequence:

>1659_bases
ATGGCTTTGGGCAAGGGACTGCGTTTACCGTCCATCACTCCGACCCGCTTGGTGCTGCTGTTTTCGCTGGCACTGGTGGC
GCTGTACAACCTGGCGACCTGGAAGGCGCTGGGAACGCTGATTACCCTGCAAGGCGCACAGAAACTGGCGTTTTTCGCGT
CCTTCGGGTTGTTCCTGTGGGCGGCAATCACCCTGCTGCTGACCCTGGTGTCGTTTCGCTGGACGCTGAAACCGGTGCTG
ACGCTGGTGGCCCTGCTCTCGGCCTGCGCGGCGTATTTCATGAACGAATACGGAATCACCATCGACACGGTGATGATCCA
GAACGTGTTCGAAACCAATCCCGGGGAAGCCACGGCGCTGTTCAGCGGCAAATTGCTGGCTTACGTGCTGTTGCTCGGCG
TGTTGCCGGCCGTGCTGATCTGGCGCTGGCCGGTGGTGTATCGGCCGTTCTTTCGGGGCTTGCTGAACAAGGTGCTGGTG
ATCGTCGCCTGTGTGCTGGTGATCGGCGCTTCGGTGGGCGCGTTCTATTCGACCTACGCACCGATCTTTCGCGAGGAAGA
CAAGCTCACCCACTTCATCAACCCGACCAACTACATCTACGCGATCAGCAAATACACCAAGCAGCGGCTGGGGATCAAAA
AGCATTTTGTGGTGCAGGCCATCGGCGAAGACGCGGTGATGAGCGCCAAGGCGGCCTCCCGCGAGAAGAAATCGCTGATG
GTGTTCGTGGTCGGCGAAACCGCCCGGGCCGACCATTTCTCGCTCAACGGCTATGAGCGCGAGACCAATCCCGAGCTGAG
CAAACTCGACATCCTCAATTTCACCCAGGTGCATTCCTGCGGCACTTCGACGGCGGTGTCGGTGCCGTGCATGTTCTCGA
TGTTCCCGCGCGAGGACTACAGCGACAAGAAAGGCAAAACCTACGAAGGCCTGCTGGACATCCTGCAACGGGCCGGCGTG
CAGGTGCTGTGGCTGGACAACAACAGCGACTGCAAGGGCACTTGCCTGCGGGTGCCGAACCGCGACATCGCGAAGAACCA
GCCGGGGCCGTTCTGCGACGGCAACAACTGCCTCGACGAAGCGCTGCTGGCCGACCTGCAAGGCTACATCGACAGCCTCA
AGGGCAACGCGATCATCGTCCTGCACGCCGACGGCAGCCACGGCCCGGAATATTACGAGCGCTACCCCAAGGACATGGAG
CGCTTCAAACCGATCTGCCACACCAACCAGCTGGGCAGTTGCAGCCGTGACGAACTGGTCAACGTCTACGACAACACGAT
CCTCTACACCGACCATTTCCTGGCCAAGGTGATCGAGCTGCTCAAGCGCAATCAGGATCATCTCGATACGTCGATGCTGT
ACGTCTCCGACCATGGCGAATCGCTGGGCGAGAACGGCCTGTACCTGCACGCCGCGCCTTATGCGCTGGCGCCGGAAGCG
CAGACCCATGTGCCGATGGTGATGTGGTTCGGCAACAACACCCTGGCGCAACTGGGCATCGACCGTGGCTGCCTGCAAGG
CAAAAGCAGCCAGCCGGATCTGAGCCACGACAACCTGTTCCACTCGGTGCTGGGGTTGTTCGAGGTCAGGACGTCGCTGT
ATCAGCCGGGGCTGGATATTTTCCATGGCTGTCGGCCAGCGATGACGGCGGGACAGTGA

Upstream 100 bases:

>100_bases
GATTTTGTTACCGGATGTTTACGGAATTTTTACCAATGCTCTTCTACAGTTACGCGCGTAACCGTATGGGTTTTCGACTG
ACGTAGAGGAAAGTATCGAA

Downstream 100 bases:

>100_bases
TCCCCTCCAGCTGCGCCAGATAGGCCCACGGGTAAATCCCGCGCTGATGGCCGTCGCTGAACACCAGTTGCAGACCGTAG
CCCTGAGCGTTCAATTCGAT

Product: hypothetical protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MALGKGLRLPSITPTRLVLLFSLALVALYNLATWKALGTLITLQGAQKLAFFASFGLFLWAAITLLLTLVSFRWTLKPVL
TLVALLSACAAYFMNEYGITIDTVMIQNVFETNPGEATALFSGKLLAYVLLLGVLPAVLIWRWPVVYRPFFRGLLNKVLV
IVACVLVIGASVGAFYSTYAPIFREEDKLTHFINPTNYIYAISKYTKQRLGIKKHFVVQAIGEDAVMSAKAASREKKSLM
VFVVGETARADHFSLNGYERETNPELSKLDILNFTQVHSCGTSTAVSVPCMFSMFPREDYSDKKGKTYEGLLDILQRAGV
QVLWLDNNSDCKGTCLRVPNRDIAKNQPGPFCDGNNCLDEALLADLQGYIDSLKGNAIIVLHADGSHGPEYYERYPKDME
RFKPICHTNQLGSCSRDELVNVYDNTILYTDHFLAKVIELLKRNQDHLDTSMLYVSDHGESLGENGLYLHAAPYALAPEA
QTHVPMVMWFGNNTLAQLGIDRGCLQGKSSQPDLSHDNLFHSVLGLFEVRTSLYQPGLDIFHGCRPAMTAGQ

Sequences:

>Translated_552_residues
MALGKGLRLPSITPTRLVLLFSLALVALYNLATWKALGTLITLQGAQKLAFFASFGLFLWAAITLLLTLVSFRWTLKPVL
TLVALLSACAAYFMNEYGITIDTVMIQNVFETNPGEATALFSGKLLAYVLLLGVLPAVLIWRWPVVYRPFFRGLLNKVLV
IVACVLVIGASVGAFYSTYAPIFREEDKLTHFINPTNYIYAISKYTKQRLGIKKHFVVQAIGEDAVMSAKAASREKKSLM
VFVVGETARADHFSLNGYERETNPELSKLDILNFTQVHSCGTSTAVSVPCMFSMFPREDYSDKKGKTYEGLLDILQRAGV
QVLWLDNNSDCKGTCLRVPNRDIAKNQPGPFCDGNNCLDEALLADLQGYIDSLKGNAIIVLHADGSHGPEYYERYPKDME
RFKPICHTNQLGSCSRDELVNVYDNTILYTDHFLAKVIELLKRNQDHLDTSMLYVSDHGESLGENGLYLHAAPYALAPEA
QTHVPMVMWFGNNTLAQLGIDRGCLQGKSSQPDLSHDNLFHSVLGLFEVRTSLYQPGLDIFHGCRPAMTAGQ
>Mature_551_residues
ALGKGLRLPSITPTRLVLLFSLALVALYNLATWKALGTLITLQGAQKLAFFASFGLFLWAAITLLLTLVSFRWTLKPVLT
LVALLSACAAYFMNEYGITIDTVMIQNVFETNPGEATALFSGKLLAYVLLLGVLPAVLIWRWPVVYRPFFRGLLNKVLVI
VACVLVIGASVGAFYSTYAPIFREEDKLTHFINPTNYIYAISKYTKQRLGIKKHFVVQAIGEDAVMSAKAASREKKSLMV
FVVGETARADHFSLNGYERETNPELSKLDILNFTQVHSCGTSTAVSVPCMFSMFPREDYSDKKGKTYEGLLDILQRAGVQ
VLWLDNNSDCKGTCLRVPNRDIAKNQPGPFCDGNNCLDEALLADLQGYIDSLKGNAIIVLHADGSHGPEYYERYPKDMER
FKPICHTNQLGSCSRDELVNVYDNTILYTDHFLAKVIELLKRNQDHLDTSMLYVSDHGESLGENGLYLHAAPYALAPEAQ
THVPMVMWFGNNTLAQLGIDRGCLQGKSSQPDLSHDNLFHSVLGLFEVRTSLYQPGLDIFHGCRPAMTAGQ

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=549, Percent_Identity=42.4408014571949, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI87082286, Length=477, Percent_Identity=25.1572327044025, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87082223, Length=267, Percent_Identity=30.3370786516854, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1790392, Length=406, Percent_Identity=23.8916256157635, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61292; Mature: 61160

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGKGLRLPSITPTRLVLLFSLALVALYNLATWKALGTLITLQGAQKLAFFASFGLFLW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHH
AAITLLLTLVSFRWTLKPVLTLVALLSACAAYFMNEYGITIDTVMIQNVFETNPGEATAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEE
FSGKLLAYVLLLGVLPAVLIWRWPVVYRPFFRGLLNKVLVIVACVLVIGASVGAFYSTYA
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIFREEDKLTHFINPTNYIYAISKYTKQRLGIKKHFVVQAIGEDAVMSAKAASREKKSLM
HHHCCCCCHHEEECCCHHEEEHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCEE
VFVVGETARADHFSLNGYERETNPELSKLDILNFTQVHSCGTSTAVSVPCMFSMFPREDY
EEEECCCCCCCCEECCCCCCCCCCCHHHEECCCHHHHHCCCCCCEEECCHHHHHCCCCCC
SDKKGKTYEGLLDILQRAGVQVLWLDNNSDCKGTCLRVPNRDIAKNQPGPFCDGNNCLDE
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHH
ALLADLQGYIDSLKGNAIIVLHADGSHGPEYYERYPKDMERFKPICHTNQLGSCSRDELV
HHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCHHHHHHHCCHHCCCCCCCCCHHHHH
NVYDNTILYTDHFLAKVIELLKRNQDHLDTSMLYVSDHGESLGENGLYLHAAPYALAPEA
HHHCCEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCEECCCC
QTHVPMVMWFGNNTLAQLGIDRGCLQGKSSQPDLSHDNLFHSVLGLFEVRTSLYQPGLDI
CCCCCEEEEECCCCHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
FHGCRPAMTAGQ
HHCCCCCCCCCC
>Mature Secondary Structure 
ALGKGLRLPSITPTRLVLLFSLALVALYNLATWKALGTLITLQGAQKLAFFASFGLFLW
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHH
AAITLLLTLVSFRWTLKPVLTLVALLSACAAYFMNEYGITIDTVMIQNVFETNPGEATAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEE
FSGKLLAYVLLLGVLPAVLIWRWPVVYRPFFRGLLNKVLVIVACVLVIGASVGAFYSTYA
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIFREEDKLTHFINPTNYIYAISKYTKQRLGIKKHFVVQAIGEDAVMSAKAASREKKSLM
HHHCCCCCHHEEECCCHHEEEHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCEE
VFVVGETARADHFSLNGYERETNPELSKLDILNFTQVHSCGTSTAVSVPCMFSMFPREDY
EEEECCCCCCCCEECCCCCCCCCCCHHHEECCCHHHHHCCCCCCEEECCHHHHHCCCCCC
SDKKGKTYEGLLDILQRAGVQVLWLDNNSDCKGTCLRVPNRDIAKNQPGPFCDGNNCLDE
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHH
ALLADLQGYIDSLKGNAIIVLHADGSHGPEYYERYPKDMERFKPICHTNQLGSCSRDELV
HHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCHHHHHHHCCHHCCCCCCCCCHHHHH
NVYDNTILYTDHFLAKVIELLKRNQDHLDTSMLYVSDHGESLGENGLYLHAAPYALAPEA
HHHCCEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCEECCCC
QTHVPMVMWFGNNTLAQLGIDRGCLQGKSSQPDLSHDNLFHSVLGLFEVRTSLYQPGLDI
CCCCCEEEEECCCCHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
FHGCRPAMTAGQ
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]