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The map label for this gene is potB [H]

Identifier: 77459365

GI number: 77459365

Start: 3608600

End: 3609517

Strand: Direct

Name: potB [H]

Synonym: Pfl01_3143

Alternate gene names: 77459365

Gene position: 3608600-3609517 (Clockwise)

Preceding gene: 77459364

Following gene: 77459366

Centisome position: 56.05

GC content: 62.64

Gene sequence:

>918_bases
ATGAGCGTCAGCAGCACTTCCCCCTCCCTCAACCGCGTGCTGTTGCTCAGCCCGGTGGTGTTGACCCTGCTCGCCCTGAT
CGCCATTCCGCTGGGGATCATGGGTTACATCAGCCTGTTGCCGCGCAACACCTACGGCGGCGTCGACTGGCAGGCTCAAT
GGCAACTGCAGAGTTATGTGCAGCTGTTCTTCCAGGAAGGCTTCGACGGCGAACTGGAGCTGAACTGGGTCTACGCCCAG
GCGCTGTTGCGCTCGGTGTTCCAGGCCAGTGGCACCACGGTGTTGTGCTTCCTGTTCGGCTTTCCGGTGGCGCTGTGGAT
GTCGAGCCTGACCCCGCGCCGGCGCAATTTGATGGTGTTGCTCATCACCATCCCGTTCTGGACCAACCTGCTGATCCGTA
ACTACGCGTGGCTGATCATCCTGCGCGAACAGGGCTGGGTCGCCCAGAGCCTCAACGCGCTCTTCCCGAGTGCCGGCGGC
ATCACCCTGCTCTACAACGACTTCGCCGTGAGCGTGGGTCTGGTCTACAGCTTCCTGCCGTTCATGATTTTGCCGATCTA
CTCGACCCTGGAAAAACTCGACTGGCGTCTGGTGGAAGCCGCCTATGACCTGGGCGCCAATCGCTGGCACGCCCTGCGCC
GGATCATCCTGCCGCTGTCGATGCCCGGCGTGATTGCCGGCGCGTTGCTGGTGTTCGTGCCGAGCCTCGGCGCCTTCATC
ACCCCGGCGATCCTCGGCGGCGGCAAGACCCTGATGATCGGCAACCTGATCCAGCAGCAATTCGGCACCGCGCGCAACTG
GCCGCTGGGCAGTTCGCTGTCGTTCCTGTTGCTGGGGATTCTGCTGTTGTCCCTGGTGCTCTACGCCCTCTACAGCCGCA
GCGCGATGAAAAACCTGAACCGGGGAGCGAAAGCATGA

Upstream 100 bases:

>100_bases
CTGCGGGAAAACAACGAAGGCCTGAACCGTGGTCGCGCAGTCGGTGAGCCCGTGGCGGTGCACCTGCCGCCCCAGGCCTG
CCTGTTGATGGAGGCCTGAG

Downstream 100 bases:

>100_bases
TCTCTTTGCACCTGAAAAAACTCCCGGCGACCCGGGAAATCAGCCTGCTGATCCTCGCTTATCTGTACGTGCCGATCCTG
GTGTTGATCGCCTACAGCTT

Product: binding-protein dependent transport system inner membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSVSSTSPSLNRVLLLSPVVLTLLALIAIPLGIMGYISLLPRNTYGGVDWQAQWQLQSYVQLFFQEGFDGELELNWVYAQ
ALLRSVFQASGTTVLCFLFGFPVALWMSSLTPRRRNLMVLLITIPFWTNLLIRNYAWLIILREQGWVAQSLNALFPSAGG
ITLLYNDFAVSVGLVYSFLPFMILPIYSTLEKLDWRLVEAAYDLGANRWHALRRIILPLSMPGVIAGALLVFVPSLGAFI
TPAILGGGKTLMIGNLIQQQFGTARNWPLGSSLSFLLLGILLLSLVLYALYSRSAMKNLNRGAKA

Sequences:

>Translated_305_residues
MSVSSTSPSLNRVLLLSPVVLTLLALIAIPLGIMGYISLLPRNTYGGVDWQAQWQLQSYVQLFFQEGFDGELELNWVYAQ
ALLRSVFQASGTTVLCFLFGFPVALWMSSLTPRRRNLMVLLITIPFWTNLLIRNYAWLIILREQGWVAQSLNALFPSAGG
ITLLYNDFAVSVGLVYSFLPFMILPIYSTLEKLDWRLVEAAYDLGANRWHALRRIILPLSMPGVIAGALLVFVPSLGAFI
TPAILGGGKTLMIGNLIQQQFGTARNWPLGSSLSFLLLGILLLSLVLYALYSRSAMKNLNRGAKA
>Mature_304_residues
SVSSTSPSLNRVLLLSPVVLTLLALIAIPLGIMGYISLLPRNTYGGVDWQAQWQLQSYVQLFFQEGFDGELELNWVYAQA
LLRSVFQASGTTVLCFLFGFPVALWMSSLTPRRRNLMVLLITIPFWTNLLIRNYAWLIILREQGWVAQSLNALFPSAGGI
TLLYNDFAVSVGLVYSFLPFMILPIYSTLEKLDWRLVEAAYDLGANRWHALRRIILPLSMPGVIAGALLVFVPSLGAFIT
PAILGGGKTLMIGNLIQQQFGTARNWPLGSSLSFLLLGILLLSLVLYALYSRSAMKNLNRGAKA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=243, Percent_Identity=40.3292181069959, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1787080, Length=226, Percent_Identity=37.1681415929204, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1787713, Length=218, Percent_Identity=31.651376146789, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787368, Length=69, Percent_Identity=44.9275362318841, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33805; Mature: 33673

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSSTSPSLNRVLLLSPVVLTLLALIAIPLGIMGYISLLPRNTYGGVDWQAQWQLQSYV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QLFFQEGFDGELELNWVYAQALLRSVFQASGTTVLCFLFGFPVALWMSSLTPRRRNLMVL
HHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEE
LITIPFWTNLLIRNYAWLIILREQGWVAQSLNALFPSAGGITLLYNDFAVSVGLVYSFLP
EEEHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
FMILPIYSTLEKLDWRLVEAAYDLGANRWHALRRIILPLSMPGVIAGALLVFVPSLGAFI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TPAILGGGKTLMIGNLIQQQFGTARNWPLGSSLSFLLLGILLLSLVLYALYSRSAMKNLN
HHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGAKA
CCCCC
>Mature Secondary Structure 
SVSSTSPSLNRVLLLSPVVLTLLALIAIPLGIMGYISLLPRNTYGGVDWQAQWQLQSYV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QLFFQEGFDGELELNWVYAQALLRSVFQASGTTVLCFLFGFPVALWMSSLTPRRRNLMVL
HHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEE
LITIPFWTNLLIRNYAWLIILREQGWVAQSLNALFPSAGGITLLYNDFAVSVGLVYSFLP
EEEHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
FMILPIYSTLEKLDWRLVEAAYDLGANRWHALRRIILPLSMPGVIAGALLVFVPSLGAFI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TPAILGGGKTLMIGNLIQQQFGTARNWPLGSSLSFLLLGILLLSLVLYALYSRSAMKNLN
HHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGAKA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]