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The map label for this gene is tcyB [H]

Identifier: 77459312

GI number: 77459312

Start: 3539222

End: 3539884

Strand: Reverse

Name: tcyB [H]

Synonym: Pfl01_3090

Alternate gene names: 77459312

Gene position: 3539884-3539222 (Counterclockwise)

Preceding gene: 77459313

Following gene: 77459311

Centisome position: 54.98

GC content: 60.18

Gene sequence:

>663_bases
ATGTACCAATCCCCCAGTTGGTTGCATGAGTTGTGGATTGCCCGCGAAGCCTTGTGGCAAGGGTTTCTGACCAGTGTGCA
GGTGTCGGCGCTGGCCATTTTGTTCGGGACGGTCATCGGCGTATTTGCCGGGCTGGTGCTGACCTACGGCAAGTTCTGGA
TGCGCGCACCGTTCCGCTTTTATGTCGACCTGATTCGCGGTACGCCGGTGTTCGTGCTGGTGTTGGCGTGCTTCTACATG
GCTCCGGCGCTTGGTTGGCAGATCAGCGCATTTCAGGCCGGCGCATTGGGTCTGACGCTGTTTTGCGGCTCCCACGTGGC
CGAAATCGTGCGCGGCGCGTTGCAGGCGTTGCCACGCGGGCAGATGGAAGCCGGCAAGGCCATCGGCCTGACGTTCTATC
AGTCCCTCGGCTACGTGCTGTTGCCTCAGGCGCTGCGGCAGATCCTGCCGACCTGGGTCAACTCGTCCACGGAAATCGTC
AAGGCCTCGACCTTGCTGTCGGTGATCGGCGTGGCCGAGCTGTTGCTCAGCACCCAGCAAATCATCGCCCGGACGTTCAT
GACCCTGGAGTTTTACCTGTTCGCCGGTTTTCTGTTCTTCGTCATCAACTACGGCATCGAATTACTCGGCCGGCACATTG
AAAAGCGGGTGGCCCTGCCATGA

Upstream 100 bases:

>100_bases
GCTGGTGACCACGGCGTTGTATGTCGCGGCCTGTTACCTCATTGCCATGCTGCTGCGTTACCTCGAGCAGCGTCTGGCGA
TCCGCCGATAGGAGGCTGCG

Downstream 100 bases:

>100_bases
CTCAAGCTCAAGTTTCCAACGTCCAGAACGGTCAGCCCTTGCTGGACATCCGTGGCCTGCACAAGCAATACGGCGCGGTC
GAAGTGCTCAAAGGCGTCGA

Product: amino acid ABC transporter permease

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MYQSPSWLHELWIAREALWQGFLTSVQVSALAILFGTVIGVFAGLVLTYGKFWMRAPFRFYVDLIRGTPVFVLVLACFYM
APALGWQISAFQAGALGLTLFCGSHVAEIVRGALQALPRGQMEAGKAIGLTFYQSLGYVLLPQALRQILPTWVNSSTEIV
KASTLLSVIGVAELLLSTQQIIARTFMTLEFYLFAGFLFFVINYGIELLGRHIEKRVALP

Sequences:

>Translated_220_residues
MYQSPSWLHELWIAREALWQGFLTSVQVSALAILFGTVIGVFAGLVLTYGKFWMRAPFRFYVDLIRGTPVFVLVLACFYM
APALGWQISAFQAGALGLTLFCGSHVAEIVRGALQALPRGQMEAGKAIGLTFYQSLGYVLLPQALRQILPTWVNSSTEIV
KASTLLSVIGVAELLLSTQQIIARTFMTLEFYLFAGFLFFVINYGIELLGRHIEKRVALP
>Mature_220_residues
MYQSPSWLHELWIAREALWQGFLTSVQVSALAILFGTVIGVFAGLVLTYGKFWMRAPFRFYVDLIRGTPVFVLVLACFYM
APALGWQISAFQAGALGLTLFCGSHVAEIVRGALQALPRGQMEAGKAIGLTFYQSLGYVLLPQALRQILPTWVNSSTEIV
KASTLLSVIGVAELLLSTQQIIARTFMTLEFYLFAGFLFFVINYGIELLGRHIEKRVALP

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787030, Length=211, Percent_Identity=32.7014218009479, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1788226, Length=193, Percent_Identity=30.0518134715026, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082239, Length=162, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1786874, Length=216, Percent_Identity=30.0925925925926, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1786873, Length=220, Percent_Identity=27.7272727272727, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787087, Length=135, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788645, Length=226, Percent_Identity=28.3185840707965, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994930, Length=212, Percent_Identity=28.7735849056604, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788646, Length=196, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787086, Length=210, Percent_Identity=24.2857142857143, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 24417; Mature: 24417

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQSPSWLHELWIAREALWQGFLTSVQVSALAILFGTVIGVFAGLVLTYGKFWMRAPFRF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
YVDLIRGTPVFVLVLACFYMAPALGWQISAFQAGALGLTLFCGSHVAEIVRGALQALPRG
HHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
QMEAGKAIGLTFYQSLGYVLLPQALRQILPTWVNSSTEIVKASTLLSVIGVAELLLSTQQ
CHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IIARTFMTLEFYLFAGFLFFVINYGIELLGRHIEKRVALP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYQSPSWLHELWIAREALWQGFLTSVQVSALAILFGTVIGVFAGLVLTYGKFWMRAPFRF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
YVDLIRGTPVFVLVLACFYMAPALGWQISAFQAGALGLTLFCGSHVAEIVRGALQALPRG
HHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
QMEAGKAIGLTFYQSLGYVLLPQALRQILPTWVNSSTEIVKASTLLSVIGVAELLLSTQQ
CHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IIARTFMTLEFYLFAGFLFFVINYGIELLGRHIEKRVALP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]