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The map label for this gene is gacA [H]

Identifier: 77459289

GI number: 77459289

Start: 3509409

End: 3510035

Strand: Reverse

Name: gacA [H]

Synonym: Pfl01_3067

Alternate gene names: 77459289

Gene position: 3510035-3509409 (Counterclockwise)

Preceding gene: 77459290

Following gene: 77459288

Centisome position: 54.52

GC content: 56.62

Gene sequence:

>627_bases
GTGGTCGATGACCATGATCTCGTTCGTACAGGCATTACACGTATGCTGGCCGACATCGATGGCCTGCAGGTGGTAGGTCA
GGCCGAGTCCGGGGAAGAATCCCTCATCAAGGCTCGTGAGTTGAAGCCCGACGTGGTGCTGATGGACGTCAAGATGCCGG
GCATCGGCGGCCTCGAAGCCACGCGCAAACTGTTGCGAAGTCATCCCGACATCAAAGTGGTCGCTGTCACTGTGTGTGAG
GAAGATCCGTTTCCTACACGGTTGTTGCAGGCTGGCGCGGCGGGTTATCTGACGAAGGGTGCCGGATTGCCGGAAATGGT
TCAGGCCATTCGCCTGGTGTTTGCCGGCCAGCGCTACATCAGCCCGCAAATTGCCCAGCAATTGGCGATCAAATCCTTCC
AGCCCACCAACGACTCACCGTTCGATGCGTTGTCGGAGCGGGAGATCCAGATTGCGTTGATGATTGTCGGCTGTCAGAAG
GTTCAGATCATTTCCGACAAATTGTGCCTGTCGCCGAAAACCGTTAACACCTACCGCTACCGCATATTCGAGAAGCTCTC
GATCAGCAGCGATGTCGAACTGACGCTTCTTGCGGTTCGTCACGGCATGGTTGATGCCAGCCTCTGA

Upstream 100 bases:

>100_bases
AATATGTCGCTTGCCGCTAGGTCTGCCCCGCACTTTTTGCTAAGGTGTCCGGCATCCCAATAAGACCATATCGCGAGGTG
TCTGCTTGATTAGGGGGCTA

Downstream 100 bases:

>100_bases
CCATGACTGAAGCATTCGATTCGGGCGCCTTTCTATCGACCGTCAGCGGGCGCCCCGGCGTGTATCGCATGTTCGACAGC
GATGCGCGTTTGCTGTACGT

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MVDDHDLVRTGITRMLADIDGLQVVGQAESGEESLIKARELKPDVVLMDVKMPGIGGLEATRKLLRSHPDIKVVAVTVCE
EDPFPTRLLQAGAAGYLTKGAGLPEMVQAIRLVFAGQRYISPQIAQQLAIKSFQPTNDSPFDALSEREIQIALMIVGCQK
VQIISDKLCLSPKTVNTYRYRIFEKLSISSDVELTLLAVRHGMVDASL

Sequences:

>Translated_208_residues
MVDDHDLVRTGITRMLADIDGLQVVGQAESGEESLIKARELKPDVVLMDVKMPGIGGLEATRKLLRSHPDIKVVAVTVCE
EDPFPTRLLQAGAAGYLTKGAGLPEMVQAIRLVFAGQRYISPQIAQQLAIKSFQPTNDSPFDALSEREIQIALMIVGCQK
VQIISDKLCLSPKTVNTYRYRIFEKLSISSDVELTLLAVRHGMVDASL
>Mature_208_residues
MVDDHDLVRTGITRMLADIDGLQVVGQAESGEESLIKARELKPDVVLMDVKMPGIGGLEATRKLLRSHPDIKVVAVTVCE
EDPFPTRLLQAGAAGYLTKGAGLPEMVQAIRLVFAGQRYISPQIAQQLAIKSFQPTNDSPFDALSEREIQIALMIVGCQK
VQIISDKLCLSPKTVNTYRYRIFEKLSISSDVELTLLAVRHGMVDASL

Specific function: Forms part of a two-component regulatory system gacA/gacA(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=207, Percent_Identity=58.4541062801932, Blast_Score=250, Evalue=4e-68,
Organism=Escherichia coli, GI1790102, Length=195, Percent_Identity=33.8461538461538, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788521, Length=197, Percent_Identity=29.9492385786802, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1788712, Length=201, Percent_Identity=25.8706467661692, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786747, Length=195, Percent_Identity=28.2051282051282, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787473, Length=180, Percent_Identity=28.3333333333333, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22788; Mature: 22788

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDDHDLVRTGITRMLADIDGLQVVGQAESGEESLIKARELKPDVVLMDVKMPGIGGLEA
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHH
TRKLLRSHPDIKVVAVTVCEEDPFPTRLLQAGAAGYLTKGAGLPEMVQAIRLVFAGQRYI
HHHHHHCCCCEEEEEEEEECCCCCHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCHHC
SPQIAQQLAIKSFQPTNDSPFDALSEREIQIALMIVGCQKVQIISDKLCLSPKTVNTYRY
CHHHHHHHHHHCCCCCCCCCCCHHCCCCEEEEEEEECCCEEEEEHHHHHCCCCCCCHHHH
RIFEKLSISSDVELTLLAVRHGMVDASL
HHHHHHCCCCCCCEEEEEEHHCCCCCCC
>Mature Secondary Structure
MVDDHDLVRTGITRMLADIDGLQVVGQAESGEESLIKARELKPDVVLMDVKMPGIGGLEA
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHH
TRKLLRSHPDIKVVAVTVCEEDPFPTRLLQAGAAGYLTKGAGLPEMVQAIRLVFAGQRYI
HHHHHHCCCCEEEEEEEEECCCCCHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCHHC
SPQIAQQLAIKSFQPTNDSPFDALSEREIQIALMIVGCQKVQIISDKLCLSPKTVNTYRY
CHHHHHHHHHHCCCCCCCCCCCHHCCCCEEEEEEEECCCEEEEEHHHHHCCCCCCCHHHH
RIFEKLSISSDVELTLLAVRHGMVDASL
HHHHHHCCCCCCCEEEEEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002569 [H]