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The map label for this gene is gnuT [H]

Identifier: 77459021

GI number: 77459021

Start: 3221373

End: 3222758

Strand: Reverse

Name: gnuT [H]

Synonym: Pfl01_2796

Alternate gene names: 77459021

Gene position: 3222758-3221373 (Counterclockwise)

Preceding gene: 77459022

Following gene: 77459020

Centisome position: 50.06

GC content: 56.85

Gene sequence:

>1386_bases
ATGACTCCCTTAGATATAGACCTCTTACTGACCGCATTGGTGAGCGTCCTGGTGCTGGTGGGGCTCATCGTGTCGCGTCT
CAAAATGCACCCGCTCCTGGCCTTGTTAGTGGTGTCCGTGGGCGTTGGCTTTGCAACCAGTATGGAGCCGGAAGCCATCG
TCTCCCACGTGATGACCGGCGCCGGAAAGACACTGGGGGCAGTAGGGGTCGTCATCGCGCTTGGAGCGATGCTAGGCAAA
ATTCTTGCTGACGCAGGCGTCACAGAGCAGATCGCCGAGGTCATCCTCAAGCGAACATCGGATCGGATGATCCCTTGGGC
CATGATGATGGTTGCATTTGTAATTGGCATTCCCATGTTTTTCGAGGTGGGCCTGGTGATCATGCTGCCGCTGATCTTCA
GCGTGGCGCGAAAGCTGGAAAGCCAGGCTCGCTTCAAAGGTTCAGCGTATGTGTATGTAGGTGTTCCGGTGATTGCGGCA
CTTGCAGCCATGCACGGGATGGTACCGCCGCACCCTGGCCCCTTGACGGCCATCGCTGCGCTCAAAACCTCGGTTGGGCC
CACAATGCTCTATGGTTTCCTTGCGGCAATTCCTGCAATGATCTTAGGAGGCCCGCTTTATGGCGCATTCATTTCGCCGC
GTATGAGCACTCGGCCGGATCAGGTTTTGCTGGATCAGTTCACCCTCGCCGAAAAAGCCGACGATCAACCTAGCACCAGC
GTGTGCCTTGGTGTGCTGGCAGCCTTGCTACCGGCGATCCTGATGCTGATTCATGCCGTCGCTGAGATGCTATTACCCAA
GGGCAATGCGATCCTGAAGATGGCTAGTTTCTTGGGCAATCCCCTGATAGCGATGCTTCTGGGCGTTCTGTTCGCAGGGG
CGAGTCTGGTACTCGTGCGGGGTGGCGATGCGGGGCAATTGCGCGAATCTCTGGGCAAGAGTCTCAAGCCAATCGCTTCA
ATCATCATGATCATCGCCGGCGGTGGTGCCTTTCAGGAGTTGCTGACCAGCGCCAAGGTGGGCGATGCCATTGTGCACCT
GACTCAACAGTCTGCGTTCCCTCCGCTGATCCTGGGCTGGTTGATTGCGATGCTGCTCTCGGTATCTACCGGTTCAGCCA
CTGTAGGTATCGTTGGTGCTGCTGGCTTGCTGGCGCCGCTTGCAGGTGCTGATCCAGGCCTCAACCTGCCTCTGCTTGCC
TTGTCCATCGGCTGCGGCTCGCTGTTCTTCAACTATGCGAACCATGCGGGCTTCTGGATGGTGAAGGAATCCTTCGGCAT
GACGATGGGCGAAGCAACCAAGACCATTTCGGTGGTTCAATCCATCGTAGCCGTGGTCGGTTTGATTGTGGTGTTGATGT
TGAATGCGGTTGTCACAGTGACTTGA

Upstream 100 bases:

>100_bases
CGATGTTTGGTTTATCGCGTGCAACGACAGGCGTTACGGTTTCAAACTTCTCTTTCAAATCTGACTGCAGTTTGAGCGTC
AGCCCAACCGAGATCCTTTC

Downstream 100 bases:

>100_bases
TTCAGAATCTGTTCGCAGGTAGTGAGTCTGCCCGGGCGTGTTAGCCGAGTGTGGGAAATAGAGTACGCTCCACAGGATGT
TTACCATCGAACGTAAATCC

Product: gluconate transporter

Products: gluconate [Cytoplasm]; L-idonate [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTPLDIDLLLTALVSVLVLVGLIVSRLKMHPLLALLVVSVGVGFATSMEPEAIVSHVMTGAGKTLGAVGVVIALGAMLGK
ILADAGVTEQIAEVILKRTSDRMIPWAMMMVAFVIGIPMFFEVGLVIMLPLIFSVARKLESQARFKGSAYVYVGVPVIAA
LAAMHGMVPPHPGPLTAIAALKTSVGPTMLYGFLAAIPAMILGGPLYGAFISPRMSTRPDQVLLDQFTLAEKADDQPSTS
VCLGVLAALLPAILMLIHAVAEMLLPKGNAILKMASFLGNPLIAMLLGVLFAGASLVLVRGGDAGQLRESLGKSLKPIAS
IIMIIAGGGAFQELLTSAKVGDAIVHLTQQSAFPPLILGWLIAMLLSVSTGSATVGIVGAAGLLAPLAGADPGLNLPLLA
LSIGCGSLFFNYANHAGFWMVKESFGMTMGEATKTISVVQSIVAVVGLIVVLMLNAVVTVT

Sequences:

>Translated_461_residues
MTPLDIDLLLTALVSVLVLVGLIVSRLKMHPLLALLVVSVGVGFATSMEPEAIVSHVMTGAGKTLGAVGVVIALGAMLGK
ILADAGVTEQIAEVILKRTSDRMIPWAMMMVAFVIGIPMFFEVGLVIMLPLIFSVARKLESQARFKGSAYVYVGVPVIAA
LAAMHGMVPPHPGPLTAIAALKTSVGPTMLYGFLAAIPAMILGGPLYGAFISPRMSTRPDQVLLDQFTLAEKADDQPSTS
VCLGVLAALLPAILMLIHAVAEMLLPKGNAILKMASFLGNPLIAMLLGVLFAGASLVLVRGGDAGQLRESLGKSLKPIAS
IIMIIAGGGAFQELLTSAKVGDAIVHLTQQSAFPPLILGWLIAMLLSVSTGSATVGIVGAAGLLAPLAGADPGLNLPLLA
LSIGCGSLFFNYANHAGFWMVKESFGMTMGEATKTISVVQSIVAVVGLIVVLMLNAVVTVT
>Mature_460_residues
TPLDIDLLLTALVSVLVLVGLIVSRLKMHPLLALLVVSVGVGFATSMEPEAIVSHVMTGAGKTLGAVGVVIALGAMLGKI
LADAGVTEQIAEVILKRTSDRMIPWAMMMVAFVIGIPMFFEVGLVIMLPLIFSVARKLESQARFKGSAYVYVGVPVIAAL
AAMHGMVPPHPGPLTAIAALKTSVGPTMLYGFLAAIPAMILGGPLYGAFISPRMSTRPDQVLLDQFTLAEKADDQPSTSV
CLGVLAALLPAILMLIHAVAEMLLPKGNAILKMASFLGNPLIAMLLGVLFAGASLVLVRGGDAGQLRESLGKSLKPIASI
IMIIAGGGAFQELLTSAKVGDAIVHLTQQSAFPPLILGWLIAMLLSVSTGSATVGIVGAAGLLAPLAGADPGLNLPLLAL
SIGCGSLFFNYANHAGFWMVKESFGMTMGEATKTISVVQSIVAVVGLIVVLMLNAVVTVT

Specific function: Transport Of Gluconate And L-Idonate. [C]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994935, Length=443, Percent_Identity=37.9232505643341, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1790716, Length=435, Percent_Identity=36.7816091954023, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI2367370, Length=431, Percent_Identity=34.1067285382831, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1788707, Length=448, Percent_Identity=31.4732142857143, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1790776, Length=437, Percent_Identity=29.0617848970252, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI48994939, Length=448, Percent_Identity=32.8125, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789097, Length=464, Percent_Identity=29.7413793103448, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 47570; Mature: 47439

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLDIDLLLTALVSVLVLVGLIVSRLKMHPLLALLVVSVGVGFATSMEPEAIVSHVMTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHC
AGKTLGAVGVVIALGAMLGKILADAGVTEQIAEVILKRTSDRMIPWAMMMVAFVIGIPMF
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FEVGLVIMLPLIFSVARKLESQARFKGSAYVYVGVPVIAALAAMHGMVPPHPGPLTAIAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCCCHHHHHHH
LKTSVGPTMLYGFLAAIPAMILGGPLYGAFISPRMSTRPDQVLLDQFTLAEKADDQPSTS
HHHCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHH
VCLGVLAALLPAILMLIHAVAEMLLPKGNAILKMASFLGNPLIAMLLGVLFAGASLVLVR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEE
GGDAGQLRESLGKSLKPIASIIMIIAGGGAFQELLTSAKVGDAIVHLTQQSAFPPLILGW
CCCHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LIAMLLSVSTGSATVGIVGAAGLLAPLAGADPGLNLPLLALSIGCGSLFFNYANHAGFWM
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
VKESFGMTMGEATKTISVVQSIVAVVGLIVVLMLNAVVTVT
EHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPLDIDLLLTALVSVLVLVGLIVSRLKMHPLLALLVVSVGVGFATSMEPEAIVSHVMTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHC
AGKTLGAVGVVIALGAMLGKILADAGVTEQIAEVILKRTSDRMIPWAMMMVAFVIGIPMF
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FEVGLVIMLPLIFSVARKLESQARFKGSAYVYVGVPVIAALAAMHGMVPPHPGPLTAIAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCCCHHHHHHH
LKTSVGPTMLYGFLAAIPAMILGGPLYGAFISPRMSTRPDQVLLDQFTLAEKADDQPSTS
HHHCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHH
VCLGVLAALLPAILMLIHAVAEMLLPKGNAILKMASFLGNPLIAMLLGVLFAGASLVLVR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEE
GGDAGQLRESLGKSLKPIASIIMIIAGGGAFQELLTSAKVGDAIVHLTQQSAFPPLILGW
CCCHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LIAMLLSVSTGSATVGIVGAAGLLAPLAGADPGLNLPLLALSIGCGSLFFNYANHAGFWM
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
VKESFGMTMGEATKTISVVQSIVAVVGLIVVLMLNAVVTVT
EHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: gluconate [Periplasm]; Proton [Periplasm]; L-idonate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] Proton [Periplasm] + L-idonate [Periplasm] = L-idonate [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]