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The map label for this gene is oprM [H]

Identifier: 77458882

GI number: 77458882

Start: 3047019

End: 3048440

Strand: Reverse

Name: oprM [H]

Synonym: Pfl01_2657

Alternate gene names: 77458882

Gene position: 3048440-3047019 (Counterclockwise)

Preceding gene: 77458883

Following gene: 77458879

Centisome position: 47.35

GC content: 63.22

Gene sequence:

>1422_bases
ATGAGTCTGAAAGTCTTTCTGCCGAGCCTGCTGGTGCTGGCCCTGAGTGCCTGCGCCGTCGGCCCTGACTACAAGACCCC
GACAACGGAGGCGGCGAACATAACGTCCGCCACCGACGGCGCCGCCGGCCAGAAGAACTTCGACCGTTCGAAATTCGAAG
GCATCTGGTGGCAGCAGTTCGACGATCCGACTCTCAACCAGTTGGTGACCCAATCGCTGCAAGGCAACCGTGAACTGCGC
GTGGCGTTCGCCCGCTGGAAAGCCGCCCGGGCGATCCGCGACGACGTCAGCAACGATGCGATGCCGACCATCACCAGCCG
CGCCAGCAGCGATCTGGCCAAAGGTCAGATTCCGGGCCAGACCACCAAACGGGTCAACAGTGAACGCTATGACCTGGGCC
TGGACATGGCGTGGGAGCTGGACCTGTTCGGACGCATCCAGCGCAATCTGGAAGCCAGCGACGCCGACCAGCAAGCGGCT
GAAGCCGATCTGTACCAACTGCAAGTCACCATGATTGCCGAACTGGTAGACGCTTACGGTCAACTGCGCGGCGCTCAATT
GCGGGAAAAGATCGCCCTGGCCAACCTGAACAACCAGCAGGAGTCGCGCAAGATCACCATCAGCCTGCGTGACGCCGGCG
TTGGCGATCAGCTCGATGTCGAACGTGCCGATGCTCGTCTGGCGTCGGTCGAAGCCAGCGTGCCGCAATTGCAGGCGGAA
CAGGCCCGGCAGAAAAACCGCATCGCCACCCTGCTGGGTGAACGTCCGGACAAACTGACCGTCGACTTGAGCCCGAAAGA
CCTGCCGGCGATTGCCAAGGCCTTGCCGATCGGTGATCCGGGCGAACTGCTGCAACGTCGTCCGGACATCCTCAGCGCCG
AACGCAAACTGGCTTCGGCCACGGCGCGTATCGGTGTGGCCAAGGCCGACCTGTTTCCTCGGGTCAGCCTCAGCGGTTTC
CTCGGCTGGACGGCCGGACGTGGCTCGCAGATCGGTTCTTCGGCGGCCAACGCCTGGGCGCTGGGCCCGAGCATTACCTG
GGCAGCGTTCGACCTCGGTAGCGTGCGGGCCCGTCTGCGCGGCGCCGATGCCGATGCCGAAGGCGCACTGGCGACCTACG
AGCAGCAAGTGTTGCTGGCACTGGAAGAGTCGGAAAACGCCTTCAGTGATTACGGCAAACGTCAGCAACGTCTGATTTCG
CTGATCCGTCAAAGCGAATCGAGCCGCAAGGCTGCCGACCTCGCCGAGATTCGCTACCGCGAAGGCACCACCGATTTCCT
GGTGCTGCTCGACGCCCAGCGTGAACGCCTGAACGCCGAAGACAGCCAGGCCCAGGCCGAAGTGGATCTGTACCGCGGCA
TCGTCGCGATCTACAAGGCTCTTGGTGGTGGCTGGCAGCCAGAGACGGTCGCCAGCAAGTAA

Upstream 100 bases:

>100_bases
CCCCTGTCTTCTATGTGCTGATCCGCAACTTCGTGGAGCGCAGTGAAGCGCGCAAAGCGGCCAAGGCGCAAAATCTTCAA
AAGCCACTGGAGGCGCACCC

Downstream 100 bases:

>100_bases
CAGTTTTTAAAGAGCTCCTTTGGTTGGCCGCAACCAACCAAATTTTTTGCCCCGCGTATCATTCGGTCGCGGGGCTTTTT
TGCGCCTGTCTTTCAGGGTT

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSLKVFLPSLLVLALSACAVGPDYKTPTTEAANITSATDGAAGQKNFDRSKFEGIWWQQFDDPTLNQLVTQSLQGNRELR
VAFARWKAARAIRDDVSNDAMPTITSRASSDLAKGQIPGQTTKRVNSERYDLGLDMAWELDLFGRIQRNLEASDADQQAA
EADLYQLQVTMIAELVDAYGQLRGAQLREKIALANLNNQQESRKITISLRDAGVGDQLDVERADARLASVEASVPQLQAE
QARQKNRIATLLGERPDKLTVDLSPKDLPAIAKALPIGDPGELLQRRPDILSAERKLASATARIGVAKADLFPRVSLSGF
LGWTAGRGSQIGSSAANAWALGPSITWAAFDLGSVRARLRGADADAEGALATYEQQVLLALEESENAFSDYGKRQQRLIS
LIRQSESSRKAADLAEIRYREGTTDFLVLLDAQRERLNAEDSQAQAEVDLYRGIVAIYKALGGGWQPETVASK

Sequences:

>Translated_473_residues
MSLKVFLPSLLVLALSACAVGPDYKTPTTEAANITSATDGAAGQKNFDRSKFEGIWWQQFDDPTLNQLVTQSLQGNRELR
VAFARWKAARAIRDDVSNDAMPTITSRASSDLAKGQIPGQTTKRVNSERYDLGLDMAWELDLFGRIQRNLEASDADQQAA
EADLYQLQVTMIAELVDAYGQLRGAQLREKIALANLNNQQESRKITISLRDAGVGDQLDVERADARLASVEASVPQLQAE
QARQKNRIATLLGERPDKLTVDLSPKDLPAIAKALPIGDPGELLQRRPDILSAERKLASATARIGVAKADLFPRVSLSGF
LGWTAGRGSQIGSSAANAWALGPSITWAAFDLGSVRARLRGADADAEGALATYEQQVLLALEESENAFSDYGKRQQRLIS
LIRQSESSRKAADLAEIRYREGTTDFLVLLDAQRERLNAEDSQAQAEVDLYRGIVAIYKALGGGWQPETVASK
>Mature_472_residues
SLKVFLPSLLVLALSACAVGPDYKTPTTEAANITSATDGAAGQKNFDRSKFEGIWWQQFDDPTLNQLVTQSLQGNRELRV
AFARWKAARAIRDDVSNDAMPTITSRASSDLAKGQIPGQTTKRVNSERYDLGLDMAWELDLFGRIQRNLEASDADQQAAE
ADLYQLQVTMIAELVDAYGQLRGAQLREKIALANLNNQQESRKITISLRDAGVGDQLDVERADARLASVEASVPQLQAEQ
ARQKNRIATLLGERPDKLTVDLSPKDLPAIAKALPIGDPGELLQRRPDILSAERKLASATARIGVAKADLFPRVSLSGFL
GWTAGRGSQIGSSAANAWALGPSITWAAFDLGSVRARLRGADADAEGALATYEQQVLLALEESENAFSDYGKRQQRLISL
IRQSESSRKAADLAEIRYREGTTDFLVLLDAQRERLNAEDSQAQAEVDLYRGIVAIYKALGGGWQPETVASK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=477, Percent_Identity=27.0440251572327, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI2367348, Length=446, Percent_Identity=21.0762331838565, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51539; Mature: 51408

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKVFLPSLLVLALSACAVGPDYKTPTTEAANITSATDGAAGQKNFDRSKFEGIWWQQF
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHCC
DDPTLNQLVTQSLQGNRELRVAFARWKAARAIRDDVSNDAMPTITSRASSDLAKGQIPGQ
CCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCH
TTKRVNSERYDLGLDMAWELDLFGRIQRNLEASDADQQAAEADLYQLQVTMIAELVDAYG
HHHHCCCCHHCCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QLRGAQLREKIALANLNNQQESRKITISLRDAGVGDQLDVERADARLASVEASVPQLQAE
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCHHHHHH
QARQKNRIATLLGERPDKLTVDLSPKDLPAIAKALPIGDPGELLQRRPDILSAERKLASA
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHH
TARIGVAKADLFPRVSLSGFLGWTAGRGSQIGSSAANAWALGPSITWAAFDLGSVRARLR
HHHHHHHHHHCCCCCCHHHHEEECCCCCCCCCCCCCCCEECCCCCEEEEECHHHHHHHHC
GADADAEGALATYEQQVLLALEESENAFSDYGKRQQRLISLIRQSESSRKAADLAEIRYR
CCCCCCCCCHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
EGTTDFLVLLDAQRERLNAEDSQAQAEVDLYRGIVAIYKALGGGWQPETVASK
CCCCEEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SLKVFLPSLLVLALSACAVGPDYKTPTTEAANITSATDGAAGQKNFDRSKFEGIWWQQF
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHCC
DDPTLNQLVTQSLQGNRELRVAFARWKAARAIRDDVSNDAMPTITSRASSDLAKGQIPGQ
CCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCH
TTKRVNSERYDLGLDMAWELDLFGRIQRNLEASDADQQAAEADLYQLQVTMIAELVDAYG
HHHHCCCCHHCCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QLRGAQLREKIALANLNNQQESRKITISLRDAGVGDQLDVERADARLASVEASVPQLQAE
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCHHHHHH
QARQKNRIATLLGERPDKLTVDLSPKDLPAIAKALPIGDPGELLQRRPDILSAERKLASA
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHH
TARIGVAKADLFPRVSLSGFLGWTAGRGSQIGSSAANAWALGPSITWAAFDLGSVRARLR
HHHHHHHHHHCCCCCCHHHHEEECCCCCCCCCCCCCCCEECCCCCEEEEECHHHHHHHHC
GADADAEGALATYEQQVLLALEESENAFSDYGKRQQRLISLIRQSESSRKAADLAEIRYR
CCCCCCCCCHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
EGTTDFLVLLDAQRERLNAEDSQAQAEVDLYRGIVAIYKALGGGWQPETVASK
CCCCEEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]