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The map label for this gene is dmlR [H]

Identifier: 77458645

GI number: 77458645

Start: 2775792

End: 2776796

Strand: Reverse

Name: dmlR [H]

Synonym: Pfl01_2420

Alternate gene names: 77458645

Gene position: 2776796-2775792 (Counterclockwise)

Preceding gene: 77458646

Following gene: 77458643

Centisome position: 43.13

GC content: 59.6

Gene sequence:

>1005_bases
ATGGCAAAGGGGCGGCCTCCTGTGAGGTCGCCCCTATACTCTTTCCACCTGACTGTTGAGACGACACCGATGGACGTCTT
CCAAAGCATGCGCTTCTTCATCGCCGTCGCCCAAAGCGGCAGCTTCACCGCCGCTGCCGAGATGCCGGACACCAATACCA
CCAACGTCTCCAAAGCGATCTCTGCTCTCGAAGCGCGCCTGCATACGCGGCTGATCAATCGCACCACCCGCCGTCTGGCC
CTGACCGAAGCCGGCGTGCGCTATCTGGAACGCTGCGAAAAAATCCTCGATGACGTGCGCGAAGCCGACGAAGAAGCCGG
CACCGCCCAGACCCTGCCCGTCGGCCGCTTGAAGATTCACACCATGTCGGCCATCGGCAACCACTACGTGATCGATGCCA
TCGCCCGCTATCGCGACATTCACCCCACGGTGATGTTCGATCTGACCCTGACCAACCGCGTCCCCGACCTGCTCGAAGAA
GGCTTCGACATGTCCATCGTGCTGGCCCGCGACCTGCCGGATTCCGGCTTCGTCGCGCAACGTTTGGGCATCACCTACAG
CATTCTTTGCGCCTCGCCCGCCTATGTGGCCAAACGCGGTCTGCCGCAAACACCGGGCGAGCTTTGCGAACACGAGTGTC
TGCGCATCGTCAACACGGTGATGCCGGTGGAAAACTGGACATTCGAAGGGCCCGAAGGCATGGAAACCATCAGCATTCCC
GTATCACCCTTCCACATCAACACCGCCGACGGCATGACCATCGCGATCAGAAAAGGCATGGGCATCGGTATTCAGCCGAT
TGCTTCGGCCGTGGATGGTTTGAGGGCGGGAACTTTGGTGCGGGTGTTGCCGGAGTACCGGCTGGAGGAGTTGAACCTGT
TCGCGATCTATCCGTCGCGCAAGTTCGTCGATGCGAAGATCAAGACCTGGGTGGAGTTTCTGAAGCAGTCGATCCCGCAA
TTGCTGGCATCGGATGAAGACATCGTTGGAAAGAAAGCGGTTTGA

Upstream 100 bases:

>100_bases
GGCGATGCGCGAGATCAAGGAAGAGATCACGACGCGGATCCGTTTCAATTCCGTGGTCGAGACCGGCGTGCCCGGTTTCA
TCCCGCGTAAAAAGTGACAC

Downstream 100 bases:

>100_bases
GTACCGCAGACATGGAGTGTCGACCCTCCATCCCCTGCGCCCGACTCAACTGGAACAGCCAGTGCCCATCACTTGATATT
GCAGAACGTGGCGCTCGCCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAKGRPPVRSPLYSFHLTVETTPMDVFQSMRFFIAVAQSGSFTAAAEMPDTNTTNVSKAISALEARLHTRLINRTTRRLA
LTEAGVRYLERCEKILDDVREADEEAGTAQTLPVGRLKIHTMSAIGNHYVIDAIARYRDIHPTVMFDLTLTNRVPDLLEE
GFDMSIVLARDLPDSGFVAQRLGITYSILCASPAYVAKRGLPQTPGELCEHECLRIVNTVMPVENWTFEGPEGMETISIP
VSPFHINTADGMTIAIRKGMGIGIQPIASAVDGLRAGTLVRVLPEYRLEELNLFAIYPSRKFVDAKIKTWVEFLKQSIPQ
LLASDEDIVGKKAV

Sequences:

>Translated_334_residues
MAKGRPPVRSPLYSFHLTVETTPMDVFQSMRFFIAVAQSGSFTAAAEMPDTNTTNVSKAISALEARLHTRLINRTTRRLA
LTEAGVRYLERCEKILDDVREADEEAGTAQTLPVGRLKIHTMSAIGNHYVIDAIARYRDIHPTVMFDLTLTNRVPDLLEE
GFDMSIVLARDLPDSGFVAQRLGITYSILCASPAYVAKRGLPQTPGELCEHECLRIVNTVMPVENWTFEGPEGMETISIP
VSPFHINTADGMTIAIRKGMGIGIQPIASAVDGLRAGTLVRVLPEYRLEELNLFAIYPSRKFVDAKIKTWVEFLKQSIPQ
LLASDEDIVGKKAV
>Mature_333_residues
AKGRPPVRSPLYSFHLTVETTPMDVFQSMRFFIAVAQSGSFTAAAEMPDTNTTNVSKAISALEARLHTRLINRTTRRLAL
TEAGVRYLERCEKILDDVREADEEAGTAQTLPVGRLKIHTMSAIGNHYVIDAIARYRDIHPTVMFDLTLTNRVPDLLEEG
FDMSIVLARDLPDSGFVAQRLGITYSILCASPAYVAKRGLPQTPGELCEHECLRIVNTVMPVENWTFEGPEGMETISIPV
SPFHINTADGMTIAIRKGMGIGIQPIASAVDGLRAGTLVRVLPEYRLEELNLFAIYPSRKFVDAKIKTWVEFLKQSIPQL
LASDEDIVGKKAV

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=31.9587628865979, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=30.5084745762712, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=29.3515358361775, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789440, Length=302, Percent_Identity=29.8013245033113, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=28.6689419795222, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=26.4604810996564, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=24.9134948096886, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789173, Length=256, Percent_Identity=25.78125, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790011, Length=319, Percent_Identity=23.1974921630094, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36988; Mature: 36857

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGRPPVRSPLYSFHLTVETTPMDVFQSMRFFIAVAQSGSFTAAAEMPDTNTTNVSKAI
CCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCHHHHHH
SALEARLHTRLINRTTRRLALTEAGVRYLERCEKILDDVREADEEAGTAQTLPVGRLKIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TMSAIGNHYVIDAIARYRDIHPTVMFDLTLTNRVPDLLEEGFDMSIVLARDLPDSGFVAQ
EHHHCCCHHHHHHHHHHHCCCCEEEEEEEEHHCCHHHHHCCCCEEEEEEECCCCCCHHHH
RLGITYSILCASPAYVAKRGLPQTPGELCEHECLRIVNTVMPVENWTFEGPEGMETISIP
HHCCEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
VSPFHINTADGMTIAIRKGMGIGIQPIASAVDGLRAGTLVRVLPEYRLEELNLFAIYPSR
CCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
KFVDAKIKTWVEFLKQSIPQLLASDEDIVGKKAV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AKGRPPVRSPLYSFHLTVETTPMDVFQSMRFFIAVAQSGSFTAAAEMPDTNTTNVSKAI
CCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCHHHHHH
SALEARLHTRLINRTTRRLALTEAGVRYLERCEKILDDVREADEEAGTAQTLPVGRLKIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TMSAIGNHYVIDAIARYRDIHPTVMFDLTLTNRVPDLLEEGFDMSIVLARDLPDSGFVAQ
EHHHCCCHHHHHHHHHHHCCCCEEEEEEEEHHCCHHHHHCCCCEEEEEEECCCCCCHHHH
RLGITYSILCASPAYVAKRGLPQTPGELCEHECLRIVNTVMPVENWTFEGPEGMETISIP
HHCCEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
VSPFHINTADGMTIAIRKGMGIGIQPIASAVDGLRAGTLVRVLPEYRLEELNLFAIYPSR
CCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
KFVDAKIKTWVEFLKQSIPQLLASDEDIVGKKAV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]