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The map label for this gene is shiA [H]

Identifier: 77458606

GI number: 77458606

Start: 2737471

End: 2739192

Strand: Reverse

Name: shiA [H]

Synonym: Pfl01_2379

Alternate gene names: 77458606

Gene position: 2739192-2737471 (Counterclockwise)

Preceding gene: 77458607

Following gene: 77458605

Centisome position: 42.54

GC content: 61.15

Gene sequence:

>1722_bases
ATGAAGTGCTCCGGCCTACAAAAACAAGCACGACGGAGAACACCCATGGCAGTACTCGACAGCATGTCCACCGGCAGTGC
CCCGCACCACAGCGTCAGCCGAGAGGAACGCAAGGTCATCTTTGCGTCGTCCCTCGGCACGGTGTTCGAGTGGTACGACT
TTTATCTCTACGGCTCCCTGGCGGCGATCATCGCCAAGCACTTCTTTGCCGGGGTGAACGAGACCACGGCGTTTATCTTC
GCCCTGCTCGCCTTTGCCGCCGGTTTTGCGGTGCGGCCGTTCGGGGCGATTGTTTTCGGACGCTTGGGTGACATGATCGG
GCGCAAGCACACGTTTCTGATCACGATTGTGATCATGGGTGTGTCGACGGCGATTGTGGGTTTCCTGCCCGGTTACGCGA
CCATCGGAGTCGCGGCGCCGATCATTCTGATTACCCTGCGCCTGCTGCAAGGCCTGGCGCTGGGCGGCGAGTACGGCGGC
GCGGCAACGTATGTGGCCGAGCATGCGCCCAAGGGCAAGCGCGGTTATTTCACTTCCTGGATTCAGACCACCGCGACCCT
GGGTCTGTTTATGTCGTTGCTGGTGATCCTCGCCTGCCGTACCGCGCTGGGCACCGAAGCGTTCGAGGCCTGGGGCTGGC
GGATTCCGTTCCTGCTGTCGATCCTGCTGCTGGCGGTCTCGGTGTACATCCGGTTGCAACTGAACGAGTCGCCGGTGTTC
AAGAAAATGAAGGAAGAAGGCAAGTCGTCGAAAGCCCCGCTGACCGAGTCCTTCGCCCGTTGGGACAACCTGAAAATCGT
GATCATGGCCCTGCTCGGCGGCACCGCAGGCCAAGCGGTGGTCTGGTACACCGGGCAGTTCTACGCGCTGTTTTTCCTGC
TGCAAACACTGAAGATCGACCCGCAGACCGCCAACCTGCTGATCGCCGGTTCGCTGCTGATCGGCACGCCGTTCTTCGTG
ATTTTCGGCAGCCTGTCCGACCGCATCGGGCGCAAGGGCATCATCATGGCCGGGTGCATTCTGGCGGCGGTGACCTACTT
CCCGATCTTCCACGCGCTGACCCAGTACGGTAACCCCGACGTATTCGTCGCCCAAGAGAAGAACCCGGTCAAAGTGATCG
CCAACCCCGACCAGTGCTCGTTCCAGTTCGATCCGGTGGGCAAGGCCAAATTCACCAGCTCGTGCGATCTGGCGAAAACC
ATCCTGGCGAAACGGGCGATCCCGTACGAAAACGTGGTGGCGGAACCGGGCACCGTGGCCCAGGTGCGTATCGGCGACAA
AGTCATCGAAAGCTTCGAAGGCACGACCCTGCCGGCCGCTGACTTCAAGACCCGTAACGACGCTTTCACCGCCAGCCTCG
GCGCCGCGCTGAAAGAAGCCGGTTACCCGGAAAAAGCCGACCCGGCCAAGACCAATTACCCGATGGTGCTGCTCCTGCTG
ACCGTTCTGGTGATCTACGTGACCATGGTCTACGGTCCGATTGCCGCGTGGCTGGTCGAGCTGTTCCCGGCGCGCATCCG
CTACACCTCGATGTCGCTGCCGTATCACATCGGCAACGGCTGGTTCGGCGGGTTCCTGCCGACGGTGGCGTTTGCGATGG
TCGCGGCCACCGGGGATATCTACTACGGCTTGTGGTACCCGATCGTGATTGCGGTGATGACGGCGATTCTGGGGATCTTC
TTCATGCCGGAGACCAAGGATCGGGACATTCATCACACCTGA

Upstream 100 bases:

>100_bases
GCTCAATGAAACCCCGCCCTCCCGCGAGGTCGTGATTCTGTGCACGCTCCACGAAATACCTGAACCGGCATCCGCCACAG
GCCTTGGGTACGCGACGTTG

Downstream 100 bases:

>100_bases
CTCAACTCTGTTCGATGCTGTGCGTGGCGTTCACGCACAGCATCGAACGTTGTCACATCGGCGTCATTCTCCCTTGGTAA
CGTCGTCGCGCCCCGATGCC

Product: general substrate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MKCSGLQKQARRRTPMAVLDSMSTGSAPHHSVSREERKVIFASSLGTVFEWYDFYLYGSLAAIIAKHFFAGVNETTAFIF
ALLAFAAGFAVRPFGAIVFGRLGDMIGRKHTFLITIVIMGVSTAIVGFLPGYATIGVAAPIILITLRLLQGLALGGEYGG
AATYVAEHAPKGKRGYFTSWIQTTATLGLFMSLLVILACRTALGTEAFEAWGWRIPFLLSILLLAVSVYIRLQLNESPVF
KKMKEEGKSSKAPLTESFARWDNLKIVIMALLGGTAGQAVVWYTGQFYALFFLLQTLKIDPQTANLLIAGSLLIGTPFFV
IFGSLSDRIGRKGIIMAGCILAAVTYFPIFHALTQYGNPDVFVAQEKNPVKVIANPDQCSFQFDPVGKAKFTSSCDLAKT
ILAKRAIPYENVVAEPGTVAQVRIGDKVIESFEGTTLPAADFKTRNDAFTASLGAALKEAGYPEKADPAKTNYPMVLLLL
TVLVIYVTMVYGPIAAWLVELFPARIRYTSMSLPYHIGNGWFGGFLPTVAFAMVAATGDIYYGLWYPIVIAVMTAILGIF
FMPETKDRDIHHT

Sequences:

>Translated_573_residues
MKCSGLQKQARRRTPMAVLDSMSTGSAPHHSVSREERKVIFASSLGTVFEWYDFYLYGSLAAIIAKHFFAGVNETTAFIF
ALLAFAAGFAVRPFGAIVFGRLGDMIGRKHTFLITIVIMGVSTAIVGFLPGYATIGVAAPIILITLRLLQGLALGGEYGG
AATYVAEHAPKGKRGYFTSWIQTTATLGLFMSLLVILACRTALGTEAFEAWGWRIPFLLSILLLAVSVYIRLQLNESPVF
KKMKEEGKSSKAPLTESFARWDNLKIVIMALLGGTAGQAVVWYTGQFYALFFLLQTLKIDPQTANLLIAGSLLIGTPFFV
IFGSLSDRIGRKGIIMAGCILAAVTYFPIFHALTQYGNPDVFVAQEKNPVKVIANPDQCSFQFDPVGKAKFTSSCDLAKT
ILAKRAIPYENVVAEPGTVAQVRIGDKVIESFEGTTLPAADFKTRNDAFTASLGAALKEAGYPEKADPAKTNYPMVLLLL
TVLVIYVTMVYGPIAAWLVELFPARIRYTSMSLPYHIGNGWFGGFLPTVAFAMVAATGDIYYGLWYPIVIAVMTAILGIF
FMPETKDRDIHHT
>Mature_573_residues
MKCSGLQKQARRRTPMAVLDSMSTGSAPHHSVSREERKVIFASSLGTVFEWYDFYLYGSLAAIIAKHFFAGVNETTAFIF
ALLAFAAGFAVRPFGAIVFGRLGDMIGRKHTFLITIVIMGVSTAIVGFLPGYATIGVAAPIILITLRLLQGLALGGEYGG
AATYVAEHAPKGKRGYFTSWIQTTATLGLFMSLLVILACRTALGTEAFEAWGWRIPFLLSILLLAVSVYIRLQLNESPVF
KKMKEEGKSSKAPLTESFARWDNLKIVIMALLGGTAGQAVVWYTGQFYALFFLLQTLKIDPQTANLLIAGSLLIGTPFFV
IFGSLSDRIGRKGIIMAGCILAAVTYFPIFHALTQYGNPDVFVAQEKNPVKVIANPDQCSFQFDPVGKAKFTSSCDLAKT
ILAKRAIPYENVVAEPGTVAQVRIGDKVIESFEGTTLPAADFKTRNDAFTASLGAALKEAGYPEKADPAKTNYPMVLLLL
TVLVIYVTMVYGPIAAWLVELFPARIRYTSMSLPYHIGNGWFGGFLPTVAFAMVAATGDIYYGLWYPIVIAVMTAILGIF
FMPETKDRDIHHT

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=346, Percent_Identity=34.393063583815, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1790550, Length=325, Percent_Identity=35.0769230769231, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788942, Length=342, Percent_Identity=34.7953216374269, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1789941, Length=222, Percent_Identity=43.6936936936937, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI87082231, Length=258, Percent_Identity=25.968992248062, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87082404, Length=326, Percent_Identity=25.4601226993865, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 62438; Mature: 62438

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCSGLQKQARRRTPMAVLDSMSTGSAPHHSVSREERKVIFASSLGTVFEWYDFYLYGSL
CCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHEEEEHHHHHHHHHHHHHHHHHH
AAIIAKHFFAGVNETTAFIFALLAFAAGFAVRPFGAIVFGRLGDMIGRKHTFLITIVIMG
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VSTAIVGFLPGYATIGVAAPIILITLRLLQGLALGGEYGGAATYVAEHAPKGKRGYFTSW
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHH
IQTTATLGLFMSLLVILACRTALGTEAFEAWGWRIPFLLSILLLAVSVYIRLQLNESPVF
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCHHH
KKMKEEGKSSKAPLTESFARWDNLKIVIMALLGGTAGQAVVWYTGQFYALFFLLQTLKID
HHHHHHCCCCCCCHHHHHHHHCCHHEEEHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCC
PQTANLLIAGSLLIGTPFFVIFGSLSDRIGRKGIIMAGCILAAVTYFPIFHALTQYGNPD
CCCCCEEEECHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
VFVAQEKNPVKVIANPDQCSFQFDPVGKAKFTSSCDLAKTILAKRAIPYENVVAEPGTVA
EEEEECCCCEEEEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCEE
QVRIGDKVIESFEGTTLPAADFKTRNDAFTASLGAALKEAGYPEKADPAKTNYPMVLLLL
EEECCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
TVLVIYVTMVYGPIAAWLVELFPARIRYTSMSLPYHIGNGWFGGFLPTVAFAMVAATGDI
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEECCCCCCHHHHHHHHHHHHHHCCCH
YYGLWYPIVIAVMTAILGIFFMPETKDRDIHHT
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCC
>Mature Secondary Structure
MKCSGLQKQARRRTPMAVLDSMSTGSAPHHSVSREERKVIFASSLGTVFEWYDFYLYGSL
CCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHEEEEHHHHHHHHHHHHHHHHHH
AAIIAKHFFAGVNETTAFIFALLAFAAGFAVRPFGAIVFGRLGDMIGRKHTFLITIVIMG
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VSTAIVGFLPGYATIGVAAPIILITLRLLQGLALGGEYGGAATYVAEHAPKGKRGYFTSW
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHH
IQTTATLGLFMSLLVILACRTALGTEAFEAWGWRIPFLLSILLLAVSVYIRLQLNESPVF
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCHHH
KKMKEEGKSSKAPLTESFARWDNLKIVIMALLGGTAGQAVVWYTGQFYALFFLLQTLKID
HHHHHHCCCCCCCHHHHHHHHCCHHEEEHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCC
PQTANLLIAGSLLIGTPFFVIFGSLSDRIGRKGIIMAGCILAAVTYFPIFHALTQYGNPD
CCCCCEEEECHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
VFVAQEKNPVKVIANPDQCSFQFDPVGKAKFTSSCDLAKTILAKRAIPYENVVAEPGTVA
EEEEECCCCEEEEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCEE
QVRIGDKVIESFEGTTLPAADFKTRNDAFTASLGAALKEAGYPEKADPAKTNYPMVLLLL
EEECCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
TVLVIYVTMVYGPIAAWLVELFPARIRYTSMSLPYHIGNGWFGGFLPTVAFAMVAATGDI
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEECCCCCCHHHHHHHHHHHHHHCCCH
YYGLWYPIVIAVMTAILGIFFMPETKDRDIHHT
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]