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The map label for this gene is glcD [H]

Identifier: 77458446

GI number: 77458446

Start: 2541956

End: 2543455

Strand: Reverse

Name: glcD [H]

Synonym: Pfl01_2219

Alternate gene names: 77458446

Gene position: 2543455-2541956 (Counterclockwise)

Preceding gene: 77458450

Following gene: 77458445

Centisome position: 39.5

GC content: 61.93

Gene sequence:

>1500_bases
ATGAACATTCTCTACGACGAACAGGTCGACGGCGTTTTGCCCGACGTCGACAAGACTGCACTTCTGCAAGCCCTGCTGCA
CCAATGGCCAGACCTGGAAGTGCTGCATCAGCGCGAAGAATTGAAGCCTTATGAATGCGATGGGCTGTCCGCCTATCGCA
CCACGCCACTGTTGGTGGTACTGCCACGACATATCGATGAAGTTCAGGGCGTGTTGCGAATCTGCCACGAACGACGTGTG
CCGGTGGTGGCCCGGGGCGCCGGGACCGGATTGTCCGGCGGCGCTCTACCGCTGGAAAAAGGTGTGCTGCTGGTGATGGC
GCGTTTCAATAACATCCTGCACATTGATCCCGCGGCGCGTACTGCACGGGTTCAACCCGGTGTGCGCAATCTGGCGATCT
CCCAGGCCGCGGCGCCGTTCGGGTTGTATTACGCGCCTGATCCTTCCTCGCAAATCGCCTGCTCCATCGGCGGCAATGTG
GCGGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACGGACTGACCGTGCATAACCTGCTCAAGGTCGAGATCCTCAGCGT
CGATGGCGAACGCCTGACATTGGGATCGGACGCGCTGGATTCCCCCGGTTTCGACCTGCTGGCACTGTTCACCGGTTCCG
AAGGCCTGCTCGGGGTGATTACCGAAGTCACGGTCAAGCTCTTGCCCAAACCGCAGACGGCCAAAGTGCTGCTGGCGGCA
TTCGACTCCGTGGAAAAGGCCGGCCGCGCAGTGGCCGACATCATCGCGGCCGGCATCATCCCCGGCGGCCTGGAAATGAT
GGATAACCTGGCGATTCGCGCGGCCGAAGACTTCATCCACGCCGGTTATCCGGTGGACGCCGAGGCGATTCTGCTCTGCG
AGCTCGATGGCGTTGAAGCCGATGTCCACGATGATTGCACGCGGGTCCGGCAAGTGCTCGAGCACGCCGGCGCCACCGAG
CTGCGCCAGGCCCGGGACGAGGCCGAGCGCGCGCGATTCTGGGCCGGACGCAAGAATGCCTTCCCCGCCGTAGGACGATT
GTCGCCGGACTATTACTGCATGGACGGCACCATCCCGCGCCGCGAGCTACCCGGCGTACTGCATGCGATTGCTCAGCTTT
CAGCTGTACATGGCTTGCGGGTTGCCAACGTCTTCCATGCCGGCGATGGCAACATGCATCCACTGATTCTGTTCGACGCC
AATCAGCCCGGCGAACTCGAACGTGCCGAAGCGCTGGGTGGCAAGATCCTCGAACTGTGTGTGAAGGTCGGCGGCAGCAT
CACCGGCGAACACGGCGTGGGCCGGGAAAAAATCAACCAGATGTGCGCGCAGTTCAACCGTGATGAGCTGACCCTGTTCC
ACGCCGTGAAAGCAGCCTTCGACCCCGGAGGCCTGCTCAATCCCGGCAAAAACATTCCGACCCTGCATCGCTGCGCCGAA
TTCGGTGCCCTGCACGTACACCATGGGCAATTGCCCTTCCCCGAACTGGAGCGGTTCTGA

Upstream 100 bases:

>100_bases
GGACCTCCGGCCGCTACGCTTTACTTCTCGGTGTCAGCAGCCCAATGATGGAAACCGGCACCGACCTCGAATAACGACAA
CGGAGGGTCGTCCAGCGATC

Downstream 100 bases:

>100_bases
TGGGCGATACCAAGGATTTCGATGACAGCGCCGCGCTGCTCGAACAGGTCAACCGAGCACTGGAAAACGCTACGCCACTG
CGCATCCAGGGCGCCAACAG

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNILYDEQVDGVLPDVDKTALLQALLHQWPDLEVLHQREELKPYECDGLSAYRTTPLLVVLPRHIDEVQGVLRICHERRV
PVVARGAGTGLSGGALPLEKGVLLVMARFNNILHIDPAARTARVQPGVRNLAISQAAAPFGLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNLLKVEILSVDGERLTLGSDALDSPGFDLLALFTGSEGLLGVITEVTVKLLPKPQTAKVLLAA
FDSVEKAGRAVADIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVDAEAILLCELDGVEADVHDDCTRVRQVLEHAGATE
LRQARDEAERARFWAGRKNAFPAVGRLSPDYYCMDGTIPRRELPGVLHAIAQLSAVHGLRVANVFHAGDGNMHPLILFDA
NQPGELERAEALGGKILELCVKVGGSITGEHGVGREKINQMCAQFNRDELTLFHAVKAAFDPGGLLNPGKNIPTLHRCAE
FGALHVHHGQLPFPELERF

Sequences:

>Translated_499_residues
MNILYDEQVDGVLPDVDKTALLQALLHQWPDLEVLHQREELKPYECDGLSAYRTTPLLVVLPRHIDEVQGVLRICHERRV
PVVARGAGTGLSGGALPLEKGVLLVMARFNNILHIDPAARTARVQPGVRNLAISQAAAPFGLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNLLKVEILSVDGERLTLGSDALDSPGFDLLALFTGSEGLLGVITEVTVKLLPKPQTAKVLLAA
FDSVEKAGRAVADIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVDAEAILLCELDGVEADVHDDCTRVRQVLEHAGATE
LRQARDEAERARFWAGRKNAFPAVGRLSPDYYCMDGTIPRRELPGVLHAIAQLSAVHGLRVANVFHAGDGNMHPLILFDA
NQPGELERAEALGGKILELCVKVGGSITGEHGVGREKINQMCAQFNRDELTLFHAVKAAFDPGGLLNPGKNIPTLHRCAE
FGALHVHHGQLPFPELERF
>Mature_499_residues
MNILYDEQVDGVLPDVDKTALLQALLHQWPDLEVLHQREELKPYECDGLSAYRTTPLLVVLPRHIDEVQGVLRICHERRV
PVVARGAGTGLSGGALPLEKGVLLVMARFNNILHIDPAARTARVQPGVRNLAISQAAAPFGLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNLLKVEILSVDGERLTLGSDALDSPGFDLLALFTGSEGLLGVITEVTVKLLPKPQTAKVLLAA
FDSVEKAGRAVADIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVDAEAILLCELDGVEADVHDDCTRVRQVLEHAGATE
LRQARDEAERARFWAGRKNAFPAVGRLSPDYYCMDGTIPRRELPGVLHAIAQLSAVHGLRVANVFHAGDGNMHPLILFDA
NQPGELERAEALGGKILELCVKVGGSITGEHGVGREKINQMCAQFNRDELTLFHAVKAAFDPGGLLNPGKNIPTLHRCAE
FGALHVHHGQLPFPELERF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=421, Percent_Identity=28.7410926365796, Blast_Score=192, Evalue=9e-49,
Organism=Homo sapiens, GI37595754, Length=444, Percent_Identity=28.3783783783784, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI119964728, Length=450, Percent_Identity=30.4444444444444, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI4501993, Length=254, Percent_Identity=26.3779527559055, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1789351, Length=499, Percent_Identity=80.9619238476954, Blast_Score=833, Evalue=0.0,
Organism=Escherichia coli, GI1787977, Length=501, Percent_Identity=25.1497005988024, Blast_Score=108, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71992373, Length=474, Percent_Identity=27.2151898734177, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI71992381, Length=431, Percent_Identity=26.6821345707657, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI71992392, Length=371, Percent_Identity=27.7628032345013, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17534361, Length=452, Percent_Identity=26.7699115044248, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17556096, Length=453, Percent_Identity=21.1920529801325, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320023, Length=460, Percent_Identity=28.0434782608696, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6320027, Length=435, Percent_Identity=27.3563218390805, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6320764, Length=465, Percent_Identity=26.6666666666667, Blast_Score=159, Evalue=9e-40,
Organism=Drosophila melanogaster, GI18921117, Length=450, Percent_Identity=28.6666666666667, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24639277, Length=450, Percent_Identity=28.6666666666667, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24639275, Length=450, Percent_Identity=28.6666666666667, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24653753, Length=222, Percent_Identity=27.9279279279279, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 53758; Mature: 53758

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILYDEQVDGVLPDVDKTALLQALLHQWPDLEVLHQREELKPYECDGLSAYRTTPLLVV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHCCCCEEEE
LPRHIDEVQGVLRICHERRVPVVARGAGTGLSGGALPLEKGVLLVMARFNNILHIDPAAR
ECHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHCCCEEEEEHHCCEEEECCCCC
TARVQPGVRNLAISQAAAPFGLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL
CCCCCCCHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCHHCCCCCEEEEECCCHHHHEE
KVEILSVDGERLTLGSDALDSPGFDLLALFTGSEGLLGVITEVTVKLLPKPQTAKVLLAA
EEEEEEECCCEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHH
FDSVEKAGRAVADIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVDAEAILLCELDGVEA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
DVHDDCTRVRQVLEHAGATELRQARDEAERARFWAGRKNAFPAVGRLSPDYYCMDGTIPR
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCH
RELPGVLHAIAQLSAVHGLRVANVFHAGDGNMHPLILFDANQPGELERAEALGGKILELC
HHCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
VKVGGSITGEHGVGREKINQMCAQFNRDELTLFHAVKAAFDPGGLLNPGKNIPTLHRCAE
HHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
FGALHVHHGQLPFPELERF
HCEEEEECCCCCCHHHHCC
>Mature Secondary Structure
MNILYDEQVDGVLPDVDKTALLQALLHQWPDLEVLHQREELKPYECDGLSAYRTTPLLVV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHCCCCEEEE
LPRHIDEVQGVLRICHERRVPVVARGAGTGLSGGALPLEKGVLLVMARFNNILHIDPAAR
ECHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHCCCEEEEEHHCCEEEECCCCC
TARVQPGVRNLAISQAAAPFGLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL
CCCCCCCHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCHHCCCCCEEEEECCCHHHHEE
KVEILSVDGERLTLGSDALDSPGFDLLALFTGSEGLLGVITEVTVKLLPKPQTAKVLLAA
EEEEEEECCCEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHH
FDSVEKAGRAVADIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVDAEAILLCELDGVEA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
DVHDDCTRVRQVLEHAGATELRQARDEAERARFWAGRKNAFPAVGRLSPDYYCMDGTIPR
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCH
RELPGVLHAIAQLSAVHGLRVANVFHAGDGNMHPLILFDANQPGELERAEALGGKILELC
HHCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
VKVGGSITGEHGVGREKINQMCAQFNRDELTLFHAVKAAFDPGGLLNPGKNIPTLHRCAE
HHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
FGALHVHHGQLPFPELERF
HCEEEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]