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The map label for this gene is glcF [H]

Identifier: 77458444

GI number: 77458444

Start: 2539656

End: 2540888

Strand: Reverse

Name: glcF [H]

Synonym: Pfl01_2217

Alternate gene names: 77458444

Gene position: 2540888-2539656 (Counterclockwise)

Preceding gene: 77458445

Following gene: 77458443

Centisome position: 39.46

GC content: 62.85

Gene sequence:

>1233_bases
ATGCAGACCACCCTCAGCCCGCAAGCCCGACAACTGCCCCGCGCCGAAGAGGCCGAAAGCATCCTGCGCACGTGCGTTCA
TTGCGGATTCTGCAACGCGACCTGCCCGACTTATCAATTGCTCGGCGATGAACTGGACGGCCCGCGAGGGCGCATCTATT
TGATCAAGCAAGTGCTCGAAGGCAACGAAGTCACGGCGAAAACCCAGCAACACCTGGATCGCTGCCTGTCGTGCCGTAAC
TGTGAAACCACCTGCCCTTCCGGCGTGGATTATCACAACCTGCTCGACATCGGTCGGGCAGTGGTCGACGCCGCCGTCCC
GCGCCCGCTCGGGCAACGACTATTGCGTGAAGGGTTGCGAAACATCGTGCCCAATCCACGATTGTTCAAAGGGTTGGTCA
TTGGCGGTCAGATGTTCCGGGCGCTACTGCCCGATGCCCTGCAAGACAAACTGCCACGCGACGCGGTGATGCCCGCATCA
CGTCCCGTCATCCGCCATGCACGGCACGTGCTGATGCTGGAGGGGTGCGTGCAGCCGGGTTTGTCACCCAACACCAATGC
AGCAGCGGCAAGGGTGCTGGATCGTCTGGGCATCAGCGTGATCCCGGCCCGCGAGGCTGGTTGTTGTGGCGCGGTGGATT
ATCACCTCGACGCCCAGGCCTCCGGCCTTGACCGCGCCCGTCACAATATTGATGCGTGGTGGCCGGCCATCGAACAGGGT
GCCGAAGCGATCGTGCAGACCGCCAGCGGTTGCGGTGCGTTCATCAAGGATTACGGGCATTTGCTCGGCGCTGATCCGGT
CTACGCCGAGAAGGCAAGACGTGTCACCACGCTCGCCCGGGATCTGGTGGAAGTGCTAAGAGACGAACCGCTTGAATCCC
TCGGCATCCACGCTGTTCAGCGCCTGGCCTTTCACTGCCCCTGCACCCTGCAGCACGCGCAGAAACTTGGCGGCGCGGTG
GAGTCGGTGCTGACTCGACTGGGCTTTAGCCTCACGTCTGTGCCGGACGCACATCTGTGCTGTGGCTCGGCGGGCACCTA
CTCGCTGACCCAGCCAACACTGGCCCGGCAGTTGCGCGAACAAAAACTCAATGCCCTGGAAAGCGGCCATCCGGACGTCA
TCGTCACCGCCAATATCGGCTGTCAGACACACCTCGACGGTGCGGGGCGCACGCCGGTCCGGCACTGGATCGAGTTGGTG
GATGCCGCGTTGCCTTCGGTTACCGAGGCGTAG

Upstream 100 bases:

>100_bases
TCTGGCGCCCGCCCTGCTGCACTATCACCGCCAACTCAAACGACAACTCGACCCCCGGGGCATTTTCAACCCCGGGCGAC
TGTACGCGGAGCTTTGAGCC

Downstream 100 bases:

>100_bases
GATCGCTGTCGAGGGCCAAACGATGGGCGAAAAAAAACCGCCTTTGCGGGGCGGTTTTTTCGACAAACCTCAGTTTGCGG
CTGGGGCTTGATGGCTCACG

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MQTTLSPQARQLPRAEEAESILRTCVHCGFCNATCPTYQLLGDELDGPRGRIYLIKQVLEGNEVTAKTQQHLDRCLSCRN
CETTCPSGVDYHNLLDIGRAVVDAAVPRPLGQRLLREGLRNIVPNPRLFKGLVIGGQMFRALLPDALQDKLPRDAVMPAS
RPVIRHARHVLMLEGCVQPGLSPNTNAAAARVLDRLGISVIPAREAGCCGAVDYHLDAQASGLDRARHNIDAWWPAIEQG
AEAIVQTASGCGAFIKDYGHLLGADPVYAEKARRVTTLARDLVEVLRDEPLESLGIHAVQRLAFHCPCTLQHAQKLGGAV
ESVLTRLGFSLTSVPDAHLCCGSAGTYSLTQPTLARQLREQKLNALESGHPDVIVTANIGCQTHLDGAGRTPVRHWIELV
DAALPSVTEA

Sequences:

>Translated_410_residues
MQTTLSPQARQLPRAEEAESILRTCVHCGFCNATCPTYQLLGDELDGPRGRIYLIKQVLEGNEVTAKTQQHLDRCLSCRN
CETTCPSGVDYHNLLDIGRAVVDAAVPRPLGQRLLREGLRNIVPNPRLFKGLVIGGQMFRALLPDALQDKLPRDAVMPAS
RPVIRHARHVLMLEGCVQPGLSPNTNAAAARVLDRLGISVIPAREAGCCGAVDYHLDAQASGLDRARHNIDAWWPAIEQG
AEAIVQTASGCGAFIKDYGHLLGADPVYAEKARRVTTLARDLVEVLRDEPLESLGIHAVQRLAFHCPCTLQHAQKLGGAV
ESVLTRLGFSLTSVPDAHLCCGSAGTYSLTQPTLARQLREQKLNALESGHPDVIVTANIGCQTHLDGAGRTPVRHWIELV
DAALPSVTEA
>Mature_410_residues
MQTTLSPQARQLPRAEEAESILRTCVHCGFCNATCPTYQLLGDELDGPRGRIYLIKQVLEGNEVTAKTQQHLDRCLSCRN
CETTCPSGVDYHNLLDIGRAVVDAAVPRPLGQRLLREGLRNIVPNPRLFKGLVIGGQMFRALLPDALQDKLPRDAVMPAS
RPVIRHARHVLMLEGCVQPGLSPNTNAAAARVLDRLGISVIPAREAGCCGAVDYHLDAQASGLDRARHNIDAWWPAIEQG
AEAIVQTASGCGAFIKDYGHLLGADPVYAEKARRVTTLARDLVEVLRDEPLESLGIHAVQRLAFHCPCTLQHAQKLGGAV
ESVLTRLGFSLTSVPDAHLCCGSAGTYSLTQPTLARQLREQKLNALESGHPDVIVTANIGCQTHLDGAGRTPVRHWIELV
DAALPSVTEA

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=404, Percent_Identity=72.5247524752475, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1786497, Length=218, Percent_Identity=25.2293577981651, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 44361; Mature: 44361

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTLSPQARQLPRAEEAESILRTCVHCGFCNATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHC
GNEVTAKTQQHLDRCLSCRNCETTCPSGVDYHNLLDIGRAVVDAAVPRPLGQRLLREGLR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NIVPNPRLFKGLVIGGQMFRALLPDALQDKLPRDAVMPASRPVIRHARHVLMLEGCVQPG
HHCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
LSPNTNAAAARVLDRLGISVIPAREAGCCGAVDYHLDAQASGLDRARHNIDAWWPAIEQG
CCCCCHHHHHHHHHHCCCEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHH
AEAIVQTASGCGAFIKDYGHLLGADPVYAEKARRVTTLARDLVEVLRDEPLESLGIHAVQ
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RLAFHCPCTLQHAQKLGGAVESVLTRLGFSLTSVPDAHLCCGSAGTYSLTQPTLARQLRE
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHCCCCCCCCCCCCHHHHHHHHH
QKLNALESGHPDVIVTANIGCQTHLDGAGRTPVRHWIELVDAALPSVTEA
HHHHHHHCCCCCEEEEECCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQTTLSPQARQLPRAEEAESILRTCVHCGFCNATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHC
GNEVTAKTQQHLDRCLSCRNCETTCPSGVDYHNLLDIGRAVVDAAVPRPLGQRLLREGLR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NIVPNPRLFKGLVIGGQMFRALLPDALQDKLPRDAVMPASRPVIRHARHVLMLEGCVQPG
HHCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
LSPNTNAAAARVLDRLGISVIPAREAGCCGAVDYHLDAQASGLDRARHNIDAWWPAIEQG
CCCCCHHHHHHHHHHCCCEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHH
AEAIVQTASGCGAFIKDYGHLLGADPVYAEKARRVTTLARDLVEVLRDEPLESLGIHAVQ
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RLAFHCPCTLQHAQKLGGAVESVLTRLGFSLTSVPDAHLCCGSAGTYSLTQPTLARQLRE
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHCCCCCCCCCCCCHHHHHHHHH
QKLNALESGHPDVIVTANIGCQTHLDGAGRTPVRHWIELVDAALPSVTEA
HHHHHHHCCCCCEEEEECCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]