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The map label for this gene is gloB

Identifier: 77458411

GI number: 77458411

Start: 2468410

End: 2469177

Strand: Reverse

Name: gloB

Synonym: Pfl01_2184

Alternate gene names: 77458411

Gene position: 2469177-2468410 (Counterclockwise)

Preceding gene: 77458417

Following gene: 77458410

Centisome position: 38.35

GC content: 61.2

Gene sequence:

>768_bases
ATGATACAGATCAGTGCCCTGCCCGCCTTCACCGACAACTACATCTGGTTGTTACAGGATCATGCCACCCAGCGTTGTGC
AGTGGTCGATCCGGGGGATGCCGCCCCGGTGCAGGCGTGGCTCGACGCGCATCCGGGCTGGGTGCTCGGCGACATCCTCA
TCACTCACCATCACCACGACCATGTCGGCGGCGTCGAGACGCTGAAAAAGGCCACCGGTGCCACGGTCTATGGACCGGCC
AGCGAAAACATTCCGGGGCGGGACGTGGCCCTCAAGGACAACGACACGGTGCGCGTGCTCGGCTGGGACTTCGATGTCTA
TGCCGTTCCCGGCCACACCCTCGGACACATCGCCTACTACCATCACGGCCTGCTGTTTTGCGGTGACACCCTGTTCGCTG
CCGGCTGCGGCCGTCTGTTCGAAGGCACGCCGCAACAAATGCACCAGTCGTTGAGCCGTCTGGCGGCGCTGCCGGAAGAC
ACGCTGGTCTACTGCACCCACGAGTACACCCTGAGCAACCTGAAATTCGCTGCTGCCGTGGAGCCGGACAACCGGGACAT
CGCCGCCCGTCTGGAAAAAGTCACCCGGCAACGTGAGAACGGTGTCATGACCCTGCCCTCGACCCTTGCGCTGGAAAAAC
TCACAAACCCGTTTTTGCGCACCGGTGAAATATTAGTTACACAAAAAGTGGACGAACGGAACGGCCCTCAGAACCGGGCG
CCGAGTGAGGTTTTTGCGGCTCTGCGGGCATGGAAAGATACGTTCTGA

Upstream 100 bases:

>100_bases
TCAGGATCGGCCTGAGCGAACGCTTTATCGATCATTGCATTCTCCAACACGCCAGGAACCTCTAAGATGCGCCAATGTTA
CCCGCTTGGCGAATTCGACG

Downstream 100 bases:

>100_bases
GTGGCTCGCCGCCTGGTACAAAAATTCTGAATGGTTGACCGCAGGGGGTGCGCTTTCTAGAATCGCCCGACATTTTTGCC
CGGAACTTACTTCCAGCCAA

Product: beta-lactamase-like protein

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MIQISALPAFTDNYIWLLQDHATQRCAVVDPGDAAPVQAWLDAHPGWVLGDILITHHHHDHVGGVETLKKATGATVYGPA
SENIPGRDVALKDNDTVRVLGWDFDVYAVPGHTLGHIAYYHHGLLFCGDTLFAAGCGRLFEGTPQQMHQSLSRLAALPED
TLVYCTHEYTLSNLKFAAAVEPDNRDIAARLEKVTRQRENGVMTLPSTLALEKLTNPFLRTGEILVTQKVDERNGPQNRA
PSEVFAALRAWKDTF

Sequences:

>Translated_255_residues
MIQISALPAFTDNYIWLLQDHATQRCAVVDPGDAAPVQAWLDAHPGWVLGDILITHHHHDHVGGVETLKKATGATVYGPA
SENIPGRDVALKDNDTVRVLGWDFDVYAVPGHTLGHIAYYHHGLLFCGDTLFAAGCGRLFEGTPQQMHQSLSRLAALPED
TLVYCTHEYTLSNLKFAAAVEPDNRDIAARLEKVTRQRENGVMTLPSTLALEKLTNPFLRTGEILVTQKVDERNGPQNRA
PSEVFAALRAWKDTF
>Mature_255_residues
MIQISALPAFTDNYIWLLQDHATQRCAVVDPGDAAPVQAWLDAHPGWVLGDILITHHHHDHVGGVETLKKATGATVYGPA
SENIPGRDVALKDNDTVRVLGWDFDVYAVPGHTLGHIAYYHHGLLFCGDTLFAAGCGRLFEGTPQQMHQSLSRLAALPED
TLVYCTHEYTLSNLKFAAAVEPDNRDIAARLEKVTRQRENGVMTLPSTLALEKLTNPFLRTGEILVTQKVDERNGPQNRA
PSEVFAALRAWKDTF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=39.9239543726236, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=39.9239543726236, Blast_Score=161, Evalue=4e-40,
Organism=Homo sapiens, GI116642887, Length=263, Percent_Identity=36.8821292775665, Blast_Score=150, Evalue=8e-37,
Organism=Homo sapiens, GI21703352, Length=263, Percent_Identity=36.8821292775665, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI14150041, Length=263, Percent_Identity=36.5019011406844, Blast_Score=144, Evalue=7e-35,
Organism=Homo sapiens, GI46361987, Length=199, Percent_Identity=37.1859296482412, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI41327741, Length=191, Percent_Identity=27.2251308900524, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1786406, Length=255, Percent_Identity=50.1960784313725, Blast_Score=224, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI17536925, Length=240, Percent_Identity=37.5, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17538952, Length=167, Percent_Identity=28.7425149700599, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320478, Length=261, Percent_Identity=29.5019157088123, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324614, Length=275, Percent_Identity=31.2727272727273, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24667711, Length=264, Percent_Identity=39.3939393939394, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI21356335, Length=264, Percent_Identity=39.3939393939394, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24667703, Length=264, Percent_Identity=39.3939393939394, Blast_Score=173, Evalue=9e-44,
Organism=Drosophila melanogaster, GI221330176, Length=165, Percent_Identity=32.1212121212121, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_PSEPF (Q3KE79)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_347916.1
- ProteinModelPortal:   Q3KE79
- SMR:   Q3KE79
- STRING:   Q3KE79
- GeneID:   3715725
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_2184
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK866802
- BioCyc:   PFLU205922:PFL_2184-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28126; Mature: 28126

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQISALPAFTDNYIWLLQDHATQRCAVVDPGDAAPVQAWLDAHPGWVLGDILITHHHHD
CEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHCCCCCEEEEEEEEEECCCC
HVGGVETLKKATGATVYGPASENIPGRDVALKDNDTVRVLGWDFDVYAVPGHTLGHIAYY
CCCHHHHHHHHCCCEEECCCCCCCCCCEEEECCCCEEEEEEECEEEEECCCCHHHHHHHH
HHGLLFCGDTLFAAGCGRLFEGTPQQMHQSLSRLAALPEDTLVYCTHEYTLSNLKFAAAV
HCCEEEECCHHHHHHCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECEECCCEEEEEEE
EPDNRDIAARLEKVTRQRENGVMTLPSTLALEKLTNPFLRTGEILVTQKVDERNGPQNRA
CCCCCHHHHHHHHHHHHHHCCEEECCHHHHHHHHCCHHHHCCCEEEEEECCCCCCCCCCC
PSEVFAALRAWKDTF
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIQISALPAFTDNYIWLLQDHATQRCAVVDPGDAAPVQAWLDAHPGWVLGDILITHHHHD
CEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHCCCCCEEEEEEEEEECCCC
HVGGVETLKKATGATVYGPASENIPGRDVALKDNDTVRVLGWDFDVYAVPGHTLGHIAYY
CCCHHHHHHHHCCCEEECCCCCCCCCCEEEECCCCEEEEEEECEEEEECCCCHHHHHHHH
HHGLLFCGDTLFAAGCGRLFEGTPQQMHQSLSRLAALPEDTLVYCTHEYTLSNLKFAAAV
HCCEEEECCHHHHHHCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECEECCCEEEEEEE
EPDNRDIAARLEKVTRQRENGVMTLPSTLALEKLTNPFLRTGEILVTQKVDERNGPQNRA
CCCCCHHHHHHHHHHHHHHCCEEECCHHHHHHHHCCHHHHCCCEEEEEECCCCCCCCCCC
PSEVFAALRAWKDTF
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA