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The map label for this gene is ycdT [H]

Identifier: 77458397

GI number: 77458397

Start: 2451349

End: 2452497

Strand: Reverse

Name: ycdT [H]

Synonym: Pfl01_2170

Alternate gene names: 77458397

Gene position: 2452497-2451349 (Counterclockwise)

Preceding gene: 77458399

Following gene: 77458396

Centisome position: 38.09

GC content: 63.36

Gene sequence:

>1149_bases
ATGGCGCTCGATCCCCTCACGATGCTCACCATTTCCACCGCCCTGGCCGCGGCGGCCGCCCTGTATCTGGCGGTCGAATG
GCGCAGCATCCGCGAATCTTCCCTGCTGTTCTGGAGCGCCGGTTTCGCCACCATCACCGTCGGCTCAAGCCTGGCCCTGT
TGCGCGCCAATGGCTATCCGCTGATCGGCATCTGGTTTGCCAACGGCCTGCTGGTGGTCGCGCACTGGCTGTTTCTGCTG
GGGGTCGCGCGATTTACCCAGCAGCGGTTGTCGCGGGCCTGGTACTTGATGTTCCTGCTCTGGCTGGCGTTGCTGCTGTT
ACCGGAAGATCCGTCGTGGTCGAAGATCATGCTGATGGCCAACTCGCTGCTGGTGGCCGCGTTGATGCTGCGGGCCAGCC
TGTTGCTGCGCCCGCACGGCAAATCGTTGAGCGTCGGCGCGGTGCAATTGCGTTATGTGTTGCTGATCCACGGCGCGTTC
TATTTCACCAAGGCGATCATCGCGGTGATTCCGGGCACGTTGATCGATCTGGCGTCGCTACGCGGCGAAATCATTCAGAT
TTCCCTGGTTGAAGGCGCGATGGCGATCATGCTGATCGCGCTTTCGATGACCGGCACCGAGCGCTACCGCCGGGAAAAGC
AGATTGCCGAACTGGCCGCTCGAGACCCGTTGACCGCGCTGTACAACCGCCGCGCCCTGGAAGTCCGCGCACCGCGCCTG
CTTGATGAAGTGTCCAGCGCCCGCCCCGGCGCGCTGCTGCTGATCGACATCGACAACTTCAAACTGGTCAACGATCTGTA
CGGCCACACCGCCGGCGACCGGTTGCTCATCGCCCTCAGCGAAATGATCCGCGCAGTGCTGCCCAATGGCGCGCTGGCGG
CACGGCTGGGCGGTGACGAATTCGTCATGCTGCTGGGCGATGCGTCCCACGCCCAGATCATGGATCTGGGCAGTGCGCTG
CGTGAGCAGTTCCAACAGATGACTTCCCAGACCTACACCACCCCGCAAGCGGTCACCCTGAGCATCGGCGCCAAGGTGTT
CGATCAGCCGCCCGCCAGCCTCGCAGCGTTGATCGAACAGGGTGACAGCGCGCTCTACGAATCCAAGCGCGGCGGTCGCA
ACCGGATTCAACTGGCCGACTACACCTGA

Upstream 100 bases:

>100_bases
CGTCTGAAAATGGAATATCCGGTGAGTGAAAATAGCAGCCCAAAGGGGGAACGCTTACAATCGTGGCCCATTAATACTAC
TCAGGCAGACCCGTTCTTTC

Downstream 100 bases:

>100_bases
CCTATCCTTGCCTGCATCCCGTAACGTCGGAGTTCGCAATGTCCTCGCCTGAATCCATCCTCCTCCAGAGCTGGCACCAC
AACGCCCAAGCCTGGACCGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MALDPLTMLTISTALAAAAALYLAVEWRSIRESSLLFWSAGFATITVGSSLALLRANGYPLIGIWFANGLLVVAHWLFLL
GVARFTQQRLSRAWYLMFLLWLALLLLPEDPSWSKIMLMANSLLVAALMLRASLLLRPHGKSLSVGAVQLRYVLLIHGAF
YFTKAIIAVIPGTLIDLASLRGEIIQISLVEGAMAIMLIALSMTGTERYRREKQIAELAARDPLTALYNRRALEVRAPRL
LDEVSSARPGALLLIDIDNFKLVNDLYGHTAGDRLLIALSEMIRAVLPNGALAARLGGDEFVMLLGDASHAQIMDLGSAL
REQFQQMTSQTYTTPQAVTLSIGAKVFDQPPASLAALIEQGDSALYESKRGGRNRIQLADYT

Sequences:

>Translated_382_residues
MALDPLTMLTISTALAAAAALYLAVEWRSIRESSLLFWSAGFATITVGSSLALLRANGYPLIGIWFANGLLVVAHWLFLL
GVARFTQQRLSRAWYLMFLLWLALLLLPEDPSWSKIMLMANSLLVAALMLRASLLLRPHGKSLSVGAVQLRYVLLIHGAF
YFTKAIIAVIPGTLIDLASLRGEIIQISLVEGAMAIMLIALSMTGTERYRREKQIAELAARDPLTALYNRRALEVRAPRL
LDEVSSARPGALLLIDIDNFKLVNDLYGHTAGDRLLIALSEMIRAVLPNGALAARLGGDEFVMLLGDASHAQIMDLGSAL
REQFQQMTSQTYTTPQAVTLSIGAKVFDQPPASLAALIEQGDSALYESKRGGRNRIQLADYT
>Mature_381_residues
ALDPLTMLTISTALAAAAALYLAVEWRSIRESSLLFWSAGFATITVGSSLALLRANGYPLIGIWFANGLLVVAHWLFLLG
VARFTQQRLSRAWYLMFLLWLALLLLPEDPSWSKIMLMANSLLVAALMLRASLLLRPHGKSLSVGAVQLRYVLLIHGAFY
FTKAIIAVIPGTLIDLASLRGEIIQISLVEGAMAIMLIALSMTGTERYRREKQIAELAARDPLTALYNRRALEVRAPRLL
DEVSSARPGALLLIDIDNFKLVNDLYGHTAGDRLLIALSEMIRAVLPNGALAARLGGDEFVMLLGDASHAQIMDLGSALR
EQFQQMTSQTYTTPQAVTLSIGAKVFDQPPASLAALIEQGDSALYESKRGGRNRIQLADYT

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=35.5029585798817, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=298, Percent_Identity=27.5167785234899, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=31.2849162011173, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=34.5911949685535, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787541, Length=186, Percent_Identity=32.7956989247312, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=34.8837209302326, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=28.8343558282209, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788956, Length=161, Percent_Identity=34.7826086956522, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787802, Length=194, Percent_Identity=29.3814432989691, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=31.547619047619, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081977, Length=157, Percent_Identity=33.1210191082803, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787816, Length=165, Percent_Identity=31.5151515151515, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081974, Length=182, Percent_Identity=27.4725274725275, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 41764; Mature: 41633

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDPLTMLTISTALAAAAALYLAVEWRSIRESSLLFWSAGFATITVGSSLALLRANGYP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCEEEEEECCCC
LIGIWFANGLLVVAHWLFLLGVARFTQQRLSRAWYLMFLLWLALLLLPEDPSWSKIMLMA
EEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
NSLLVAALMLRASLLLRPHGKSLSVGAVQLRYVLLIHGAFYFTKAIIAVIPGTLIDLASL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
RGEIIQISLVEGAMAIMLIALSMTGTERYRREKQIAELAARDPLTALYNRRALEVRAPRL
CCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHCCCHHEEHHHHH
LDEVSSARPGALLLIDIDNFKLVNDLYGHTAGDRLLIALSEMIRAVLPNGALAARLGGDE
HHHHHCCCCCEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCE
FVMLLGDASHAQIMDLGSALREQFQQMTSQTYTTPQAVTLSIGAKVFDQPPASLAALIEQ
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHCCCHHHHHHHHHC
GDSALYESKRGGRNRIQLADYT
CCHHHHHHCCCCCCEEEEECCC
>Mature Secondary Structure 
ALDPLTMLTISTALAAAAALYLAVEWRSIRESSLLFWSAGFATITVGSSLALLRANGYP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCEEEEEECCCC
LIGIWFANGLLVVAHWLFLLGVARFTQQRLSRAWYLMFLLWLALLLLPEDPSWSKIMLMA
EEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
NSLLVAALMLRASLLLRPHGKSLSVGAVQLRYVLLIHGAFYFTKAIIAVIPGTLIDLASL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
RGEIIQISLVEGAMAIMLIALSMTGTERYRREKQIAELAARDPLTALYNRRALEVRAPRL
CCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHCCCHHEEHHHHH
LDEVSSARPGALLLIDIDNFKLVNDLYGHTAGDRLLIALSEMIRAVLPNGALAARLGGDE
HHHHHCCCCCEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCE
FVMLLGDASHAQIMDLGSALREQFQQMTSQTYTTPQAVTLSIGAKVFDQPPASLAALIEQ
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHCCCHHHHHHHHHC
GDSALYESKRGGRNRIQLADYT
CCHHHHHHCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]