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The map label for this gene is potF [H]

Identifier: 77458382

GI number: 77458382

Start: 2437895

End: 2438992

Strand: Direct

Name: potF [H]

Synonym: Pfl01_2155

Alternate gene names: 77458382

Gene position: 2437895-2438992 (Clockwise)

Preceding gene: 77458381

Following gene: 77458386

Centisome position: 37.86

GC content: 59.93

Gene sequence:

>1098_bases
ATGGGCCTGCACAACCTGAGTAAAGCGTTATTGCTGGTGCTTGCACCCCTGTGTGTGCAGGCTGCCGAGACGGCGAAACC
GGTGGTCAATCTGTATATCTGGGGCGAGTACCTGGCCCCGGATACCCTGAAGAATTTCGAGGCAAAAACCGGGATTCGGG
TGGTCGCCGATCACTTCGACTCGCTGGAAACCGTCGAGACCAAACTGCTCACCGGCCGCAGCGGCTACGACCTGGTGCTG
ACCGCCGGTCAGCACCTGTCGCGGGCCATCGAGAGCGGCGCGATCCAGACCCTGAACAAGCAGCAGCTCCCGCACTTTGC
CGGGGTTGGTGACGAGTTCCGTCAGCATATGGCGGTGTTCGATCCGGGCAACCGCTACGCCGGGATCTATGCCTGGGGCA
CCACCGGTGTCGGTTATCAGGAGGAGGCCGTGAAGCAGCGCCTGCCGGACGCGCCGAGGGACAGTTGGGCGATGCTGTTC
GATCCGGCGGTGGTGTCGAAATTCGCCGATTGCGGGGTGAGCCTGCTCAACGATCCGAACGAAGTGTTCGCGGCGGTCAT
GAAGTACATGGGCCTGGACATCAATCGCCAGAGCCTCGACGACCTGAAACTCGCCGAACAGCAACTGGCGAAGATCCGCC
CGTACATCCGCTACTTCGACAATGACCTGAACATCAGCGACCTGGCCAACGGCAACATCTGCGTGGCGATGTCGTGGAAC
GGCAACGTTGCCATCGCGGCAGGTCAGGCTGAAGCGGCCCGCAAACCGTTCAAGTTGAATTACCGGATTCCGAAGGAGGG
CACGTTGATCTGGTTCGACGCGATGGTCATACCCAAGGATGCACCGCATCCCGAGGCCGGATTGGCGCTGATGGATTACC
TGATGACGCCCGAGGTGATCGCACCGATTACCGATACGATTCATTACGCCAATGCGATTACGGCGGCGGACGGGTTGGTT
GATCCGGCGATACGCAATGATCCGGGGACTTATCCGCCGGAGGCGGTGAAGGCTTCGTTGTATAGCAAGAATGACAATGG
CAAGGCGTTCAACCGGGCTTTGATTCGCGCGTTCAGTCGGTTGAAATCCGGTCTGTAG

Upstream 100 bases:

>100_bases
TCGTCGGCATCGAAAACTCCCGGGAAATTCTGCTCGGTGGCGGCAATGTCGCGTGCATCACCATGCCGCAATACGCCGGC
CAGAAAAAAGGAGCCTGACC

Downstream 100 bases:

>100_bases
CCCAAGCCCCTCACCCCGGCCCTCTCCCAAAGGGAGAGGGAGCCGACCGAGGTGTCCTGCGTCTTACATCGACCTGACAC
ACCGTGTCGATTATGGATTC

Product: putative spermidine/putrescine transport system substrate-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MGLHNLSKALLLVLAPLCVQAAETAKPVVNLYIWGEYLAPDTLKNFEAKTGIRVVADHFDSLETVETKLLTGRSGYDLVL
TAGQHLSRAIESGAIQTLNKQQLPHFAGVGDEFRQHMAVFDPGNRYAGIYAWGTTGVGYQEEAVKQRLPDAPRDSWAMLF
DPAVVSKFADCGVSLLNDPNEVFAAVMKYMGLDINRQSLDDLKLAEQQLAKIRPYIRYFDNDLNISDLANGNICVAMSWN
GNVAIAAGQAEAARKPFKLNYRIPKEGTLIWFDAMVIPKDAPHPEAGLALMDYLMTPEVIAPITDTIHYANAITAADGLV
DPAIRNDPGTYPPEAVKASLYSKNDNGKAFNRALIRAFSRLKSGL

Sequences:

>Translated_365_residues
MGLHNLSKALLLVLAPLCVQAAETAKPVVNLYIWGEYLAPDTLKNFEAKTGIRVVADHFDSLETVETKLLTGRSGYDLVL
TAGQHLSRAIESGAIQTLNKQQLPHFAGVGDEFRQHMAVFDPGNRYAGIYAWGTTGVGYQEEAVKQRLPDAPRDSWAMLF
DPAVVSKFADCGVSLLNDPNEVFAAVMKYMGLDINRQSLDDLKLAEQQLAKIRPYIRYFDNDLNISDLANGNICVAMSWN
GNVAIAAGQAEAARKPFKLNYRIPKEGTLIWFDAMVIPKDAPHPEAGLALMDYLMTPEVIAPITDTIHYANAITAADGLV
DPAIRNDPGTYPPEAVKASLYSKNDNGKAFNRALIRAFSRLKSGL
>Mature_364_residues
GLHNLSKALLLVLAPLCVQAAETAKPVVNLYIWGEYLAPDTLKNFEAKTGIRVVADHFDSLETVETKLLTGRSGYDLVLT
AGQHLSRAIESGAIQTLNKQQLPHFAGVGDEFRQHMAVFDPGNRYAGIYAWGTTGVGYQEEAVKQRLPDAPRDSWAMLFD
PAVVSKFADCGVSLLNDPNEVFAAVMKYMGLDINRQSLDDLKLAEQQLAKIRPYIRYFDNDLNISDLANGNICVAMSWNG
NVAIAAGQAEAARKPFKLNYRIPKEGTLIWFDAMVIPKDAPHPEAGLALMDYLMTPEVIAPITDTIHYANAITAADGLVD
PAIRNDPGTYPPEAVKASLYSKNDNGKAFNRALIRAFSRLKSGL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=353, Percent_Identity=40.5099150141643, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1787367, Length=336, Percent_Identity=30.0595238095238, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39981; Mature: 39850

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLHNLSKALLLVLAPLCVQAAETAKPVVNLYIWGEYLAPDTLKNFEAKTGIRVVADHFD
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHCHHHCCHHHHHHHHH
SLETVETKLLTGRSGYDLVLTAGQHLSRAIESGAIQTLNKQQLPHFAGVGDEFRQHMAVF
HHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHEEEE
DPGNRYAGIYAWGTTGVGYQEEAVKQRLPDAPRDSWAMLFDPAVVSKFADCGVSLLNDPN
CCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHCCHHHHCCHH
EVFAAVMKYMGLDINRQSLDDLKLAEQQLAKIRPYIRYFDNDLNISDLANGNICVAMSWN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEECC
GNVAIAAGQAEAARKPFKLNYRIPKEGTLIWFDAMVIPKDAPHPEAGLALMDYLMTPEVI
CCEEEEECCHHHHCCCEEEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCHHH
APITDTIHYANAITAADGLVDPAIRNDPGTYPPEAVKASLYSKNDNGKAFNRALIRAFSR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LKSGL
HHHCC
>Mature Secondary Structure 
GLHNLSKALLLVLAPLCVQAAETAKPVVNLYIWGEYLAPDTLKNFEAKTGIRVVADHFD
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHCHHHCCHHHHHHHHH
SLETVETKLLTGRSGYDLVLTAGQHLSRAIESGAIQTLNKQQLPHFAGVGDEFRQHMAVF
HHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHEEEE
DPGNRYAGIYAWGTTGVGYQEEAVKQRLPDAPRDSWAMLFDPAVVSKFADCGVSLLNDPN
CCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHCCHHHHCCHH
EVFAAVMKYMGLDINRQSLDDLKLAEQQLAKIRPYIRYFDNDLNISDLANGNICVAMSWN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEECC
GNVAIAAGQAEAARKPFKLNYRIPKEGTLIWFDAMVIPKDAPHPEAGLALMDYLMTPEVI
CCEEEEECCHHHHCCCEEEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCHHH
APITDTIHYANAITAADGLVDPAIRNDPGTYPPEAVKASLYSKNDNGKAFNRALIRAFSR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LKSGL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]