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The map label for this gene is gcvA [H]

Identifier: 77458380

GI number: 77458380

Start: 2435793

End: 2436677

Strand: Direct

Name: gcvA [H]

Synonym: Pfl01_2153

Alternate gene names: 77458380

Gene position: 2435793-2436677 (Clockwise)

Preceding gene: 77458378

Following gene: 77458381

Centisome position: 37.83

GC content: 65.65

Gene sequence:

>885_bases
ATGCGCCGACTACCTTCCCTGGCCGCCCTGCGAACTTTCGAGTGTGCCGCCCGCCACGCGCATTTCGGCCGCGCCGCCGC
TGAGCTGTGCGTCACCGACAGCGCCGTCAGCCATCAGATCCGCCAGCTCGAAGAGCAACTGGGCGTGTCGCTGTTCATCC
GCGAGGGCCGGCAGATTCGCCCGACCATCGCCGCCGGGCGTCTGATGCAGAGCCTGCAACAGGCCTTCGAATTGATCGGC
GATGCCTGCGATGAATTGCGCGATCCGTCCTCGCTCGCCGTGTTGCGGCTGGCGGTCACGGCGGAGCTGGCGCAGAAGTG
GCTGATGAACCGTCTCACCGATTTCTACGCGCGTTATCCGCACATCACCTTGCACCTCTACGAACAACCGATCGACGCCA
CGGCGCCCGGCGAAGACATCGATCTGGCGATCACCTACGGCACCGGCCCGGTGGACAGCAGCGCGTATTTCGTCCGTCCG
TTGCCGGCGTTGCAGTTCTTCCCGGTGTGCAGCCCCGGCCTGTTCAACCAGGGCATCCTGAAAACCCCCAAGGACCTGGC
CCGGCATTGCCTGTTGCATGACGATCAGGACGGCAAGACCTGGACCGCGTGGCTCACCAGTCATGCCGGCGATTTGCGCC
CGGAACGACAGTTGTATTTCGCCCACGCCGGCCTGGCGCTGGAGGCGGCGGCGCAAGGGCAGGGCGTGGCAATGGGCGAC
AACCTCACGGCGCAGGAGGACTTGCAGAACGGGCGTCTGGTGCGACCGTTCACCTCCAGCATGACCGCGCTGGGGCAGTA
TTCGCTGGTCTGCGAGCGGGTGCGGCTGGAGCGCCCGGCGGTGGCGCAGATGCTGGAATGGTTCAACGATCAACTGGCGG
ATTGA

Upstream 100 bases:

>100_bases
CGGTGCGGTTCAGCCTAGAAGAGATCTCTTGCCTGTGCCGCCGCTATGGACGCCTGCGAAAAGCCCGGTCAGCATGTCCG
CAAAAAAGGGATCCACACCC

Downstream 100 bases:

>100_bases
TGAGTTGAATTCAACTGTTCCGAAATAATCATCGTTGGCGGGCCGACTGCCCGGGACCGTAGCCTGAGGTCATTCCCATC
CCGATGACGAGGTTTGACCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MRRLPSLAALRTFECAARHAHFGRAAAELCVTDSAVSHQIRQLEEQLGVSLFIREGRQIRPTIAAGRLMQSLQQAFELIG
DACDELRDPSSLAVLRLAVTAELAQKWLMNRLTDFYARYPHITLHLYEQPIDATAPGEDIDLAITYGTGPVDSSAYFVRP
LPALQFFPVCSPGLFNQGILKTPKDLARHCLLHDDQDGKTWTAWLTSHAGDLRPERQLYFAHAGLALEAAAQGQGVAMGD
NLTAQEDLQNGRLVRPFTSSMTALGQYSLVCERVRLERPAVAQMLEWFNDQLAD

Sequences:

>Translated_294_residues
MRRLPSLAALRTFECAARHAHFGRAAAELCVTDSAVSHQIRQLEEQLGVSLFIREGRQIRPTIAAGRLMQSLQQAFELIG
DACDELRDPSSLAVLRLAVTAELAQKWLMNRLTDFYARYPHITLHLYEQPIDATAPGEDIDLAITYGTGPVDSSAYFVRP
LPALQFFPVCSPGLFNQGILKTPKDLARHCLLHDDQDGKTWTAWLTSHAGDLRPERQLYFAHAGLALEAAAQGQGVAMGD
NLTAQEDLQNGRLVRPFTSSMTALGQYSLVCERVRLERPAVAQMLEWFNDQLAD
>Mature_294_residues
MRRLPSLAALRTFECAARHAHFGRAAAELCVTDSAVSHQIRQLEEQLGVSLFIREGRQIRPTIAAGRLMQSLQQAFELIG
DACDELRDPSSLAVLRLAVTAELAQKWLMNRLTDFYARYPHITLHLYEQPIDATAPGEDIDLAITYGTGPVDSSAYFVRP
LPALQFFPVCSPGLFNQGILKTPKDLARHCLLHDDQDGKTWTAWLTSHAGDLRPERQLYFAHAGLALEAAAQGQGVAMGD
NLTAQEDLQNGRLVRPFTSSMTALGQYSLVCERVRLERPAVAQMLEWFNDQLAD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=36.551724137931, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=32.0819112627986, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786448, Length=269, Percent_Identity=33.457249070632, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI157672245, Length=167, Percent_Identity=37.125748502994, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787128, Length=254, Percent_Identity=26.3779527559055, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=249, Percent_Identity=26.1044176706827, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=272, Percent_Identity=25.7352941176471, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081978, Length=263, Percent_Identity=26.615969581749, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=26.158940397351, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32656; Mature: 32656

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50931 HTH_LYSR ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLPSLAALRTFECAARHAHFGRAAAELCVTDSAVSHQIRQLEEQLGVSLFIREGRQIR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCC
PTIAAGRLMQSLQQAFELIGDACDELRDPSSLAVLRLAVTAELAQKWLMNRLTDFYARYP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HITLHLYEQPIDATAPGEDIDLAITYGTGPVDSSAYFVRPLPALQFFPVCSPGLFNQGIL
CEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCHHCCCCCCCCCCCCCCC
KTPKDLARHCLLHDDQDGKTWTAWLTSHAGDLRPERQLYFAHAGLALEAAAQGQGVAMGD
CCHHHHHHHHCCCCCCCCCEEEEEHHHCCCCCCCCHHEEEEECCCCEEHHCCCCCEEECC
NLTAQEDLQNGRLVRPFTSSMTALGQYSLVCERVRLERPAVAQMLEWFNDQLAD
CCCHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRLPSLAALRTFECAARHAHFGRAAAELCVTDSAVSHQIRQLEEQLGVSLFIREGRQIR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCC
PTIAAGRLMQSLQQAFELIGDACDELRDPSSLAVLRLAVTAELAQKWLMNRLTDFYARYP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HITLHLYEQPIDATAPGEDIDLAITYGTGPVDSSAYFVRPLPALQFFPVCSPGLFNQGIL
CEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCHHCCCCCCCCCCCCCCC
KTPKDLARHCLLHDDQDGKTWTAWLTSHAGDLRPERQLYFAHAGLALEAAAQGQGVAMGD
CCHHHHHHHHCCCCCCCCCEEEEEHHHCCCCCCCCHHEEEEECCCCEEHHCCCCCEEECC
NLTAQEDLQNGRLVRPFTSSMTALGQYSLVCERVRLERPAVAQMLEWFNDQLAD
CCCHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]