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The map label for this gene is puuP [H]

Identifier: 77458282

GI number: 77458282

Start: 2342491

End: 2343840

Strand: Reverse

Name: puuP [H]

Synonym: Pfl01_2055

Alternate gene names: 77458282

Gene position: 2343840-2342491 (Counterclockwise)

Preceding gene: 77458283

Following gene: 77458281

Centisome position: 36.4

GC content: 60.07

Gene sequence:

>1350_bases
ATGGAAATAGAAGAGTTCGGCTACAAACAGGAGTTGAAACGTAGCCTGACACTGACCGATCTGGTGGTGTACGGGATGAT
CTTCATGATCCCCATCGCCCCGTTCGGCGTGTATGGCTACGTCAACGCCGAGGCACCGGGGATGGTGCCTCTGGCGTACA
TCATCGGCATGGTGGCGATGGTTTTCACCGCGCTGAGTTACGGCAGCATGGCCCGGGCCTTTCCGATTGCCGGATCGGTG
TATTCCTACGCGCAACGGGGCTTGAATCAACATGTCGGTTTCATCGCCGGCTGGCTGATGCTGCTCGACTATCTGCTGAT
TCCGCCGCTGCTTTACGTCTACGCGGCGATGGCGCTCAACCACTTGTACCCGGACATCCCGAAGGTCGGGTTCATTCTGG
CGTTTCTGGTCAGCGCGACCTTCGTCAACCTGCGCGGCATCACCTTTACCGCTCGCATGAACATTGTGTTCCTGCTGGCG
CAACTCGTGGTGCTGGGGATTTTCCTGTTCTACGCCTGGAACGCTCTGCACAACGGTGGCGGTAACGGCCAGTTGACCCT
CGCGCCGCTGTATCACCCGGAAACCTTCAACTTCGCCCTGCTGATGCAAGCGGTGTCGATTGCGGTGCTGTCGTTCCTCG
GCTTCGATGCGATCTCGACCCTCGCCGAAGAGATCAAGGGCGACCCGGGCCGCAGCGTCGGCAAAGCGGCGCTGATCACC
CTGGTGGTGATGGGGGTGATTTTCGTCGCGCAAACCTGGATCGCTACCGATCTGGCAGCCGGCATGGGCTTCAAGTCCGC
CGATACCGCGTTCTATGAAATCGCCGAAATCGCTGCCGGCAGCTGGCTGGCGACCCTGACCGGTGTCGCTACCGCTTTGG
CCTGGGGCGTGGCGGTAGCGATCACCTCGCAAGCAGCGGTCTCGCGCCTGCTGTTCGGCATGGCCCGCGACGGCAAGCTG
CCGAAAGTGCTGGCCAAGGTGCACCCGAAACACAACACGCCGTACCTGAGCATTTATCTGGTGGCCGTGCTTTCTCTGGT
GATCTGCTACCTGTTCATCAATTCGGTGGACACCCTGACCTCGCTGGTCAACTTCGGCGCCCTGAGCGGCTTCATGCTGC
TGCACCTGACCGTGATCAATTACTACTGGCGCCGGCAGAAATCCGGACAGGTGATCCGTCACCTGATCTGCCCGGTGATC
GGCTTCATCATCGTCGCGGCCATCATGTACAACATGGGCGTCGATGCACAGAAACTCGGCCTGATCTGGATCGCACTGGG
TCTGGTGTACCTGTTCTTCCTGAACAAGCTCGGCGCCAGTACCGCGCTGCCTGACCCGAGCAACGGCTGA

Upstream 100 bases:

>100_bases
TCCATTGCTGTAAGGATTGTTGTAACCACTGCTGACGCTGTAAACGACGGACCGATATAACAACTAAAACGACCGATTGG
CTGACAAGGAGTTGCGCTTC

Downstream 100 bases:

>100_bases
CAAGAAAAAGAGCGGCGTCTGACAACAAATCAGGCGACCGCCGCTTTAACGCGTGGAAACCGACAGTGATAGTCAGGTTC
GGCGAATGGGCCGAACCTTT

Product: amino acid permease-associated protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MEIEEFGYKQELKRSLTLTDLVVYGMIFMIPIAPFGVYGYVNAEAPGMVPLAYIIGMVAMVFTALSYGSMARAFPIAGSV
YSYAQRGLNQHVGFIAGWLMLLDYLLIPPLLYVYAAMALNHLYPDIPKVGFILAFLVSATFVNLRGITFTARMNIVFLLA
QLVVLGIFLFYAWNALHNGGGNGQLTLAPLYHPETFNFALLMQAVSIAVLSFLGFDAISTLAEEIKGDPGRSVGKAALIT
LVVMGVIFVAQTWIATDLAAGMGFKSADTAFYEIAEIAAGSWLATLTGVATALAWGVAVAITSQAAVSRLLFGMARDGKL
PKVLAKVHPKHNTPYLSIYLVAVLSLVICYLFINSVDTLTSLVNFGALSGFMLLHLTVINYYWRRQKSGQVIRHLICPVI
GFIIVAAIMYNMGVDAQKLGLIWIALGLVYLFFLNKLGASTALPDPSNG

Sequences:

>Translated_449_residues
MEIEEFGYKQELKRSLTLTDLVVYGMIFMIPIAPFGVYGYVNAEAPGMVPLAYIIGMVAMVFTALSYGSMARAFPIAGSV
YSYAQRGLNQHVGFIAGWLMLLDYLLIPPLLYVYAAMALNHLYPDIPKVGFILAFLVSATFVNLRGITFTARMNIVFLLA
QLVVLGIFLFYAWNALHNGGGNGQLTLAPLYHPETFNFALLMQAVSIAVLSFLGFDAISTLAEEIKGDPGRSVGKAALIT
LVVMGVIFVAQTWIATDLAAGMGFKSADTAFYEIAEIAAGSWLATLTGVATALAWGVAVAITSQAAVSRLLFGMARDGKL
PKVLAKVHPKHNTPYLSIYLVAVLSLVICYLFINSVDTLTSLVNFGALSGFMLLHLTVINYYWRRQKSGQVIRHLICPVI
GFIIVAAIMYNMGVDAQKLGLIWIALGLVYLFFLNKLGASTALPDPSNG
>Mature_449_residues
MEIEEFGYKQELKRSLTLTDLVVYGMIFMIPIAPFGVYGYVNAEAPGMVPLAYIIGMVAMVFTALSYGSMARAFPIAGSV
YSYAQRGLNQHVGFIAGWLMLLDYLLIPPLLYVYAAMALNHLYPDIPKVGFILAFLVSATFVNLRGITFTARMNIVFLLA
QLVVLGIFLFYAWNALHNGGGNGQLTLAPLYHPETFNFALLMQAVSIAVLSFLGFDAISTLAEEIKGDPGRSVGKAALIT
LVVMGVIFVAQTWIATDLAAGMGFKSADTAFYEIAEIAAGSWLATLTGVATALAWGVAVAITSQAAVSRLLFGMARDGKL
PKVLAKVHPKHNTPYLSIYLVAVLSLVICYLFINSVDTLTSLVNFGALSGFMLLHLTVINYYWRRQKSGQVIRHLICPVI
GFIIVAAIMYNMGVDAQKLGLIWIALGLVYLFFLNKLGASTALPDPSNG

Specific function: Imports putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI4507047, Length=427, Percent_Identity=22.9508196721311, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI115648063, Length=400, Percent_Identity=24.5, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI115648022, Length=400, Percent_Identity=24.5, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI87081869, Length=443, Percent_Identity=34.7629796839729, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI87082023, Length=442, Percent_Identity=35.2941176470588, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1786302, Length=402, Percent_Identity=23.8805970149254, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082250, Length=405, Percent_Identity=25.4320987654321, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789017, Length=323, Percent_Identity=24.1486068111455, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1786789, Length=380, Percent_Identity=22.8947368421053, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=415, Percent_Identity=23.3734939759036, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17533459, Length=406, Percent_Identity=22.6600985221675, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24668806, Length=421, Percent_Identity=22.8028503562945, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356285, Length=421, Percent_Identity=22.8028503562945, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24668802, Length=421, Percent_Identity=22.8028503562945, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221512776, Length=428, Percent_Identity=20.7943925233645, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24666159, Length=428, Percent_Identity=20.7943925233645, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24667468, Length=410, Percent_Identity=22.9268292682927, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 48673; Mature: 48673

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIEEFGYKQELKRSLTLTDLVVYGMIFMIPIAPFGVYGYVNAEAPGMVPLAYIIGMVAM
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHH
VFTALSYGSMARAFPIAGSVYSYAQRGLNQHVGFIAGWLMLLDYLLIPPLLYVYAAMALN
HHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLYPDIPKVGFILAFLVSATFVNLRGITFTARMNIVFLLAQLVVLGIFLFYAWNALHNGG
HHCCCCCHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GNGQLTLAPLYHPETFNFALLMQAVSIAVLSFLGFDAISTLAEEIKGDPGRSVGKAALIT
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LVVMGVIFVAQTWIATDLAAGMGFKSADTAFYEIAEIAAGSWLATLTGVATALAWGVAVA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ITSQAAVSRLLFGMARDGKLPKVLAKVHPKHNTPYLSIYLVAVLSLVICYLFINSVDTLT
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SLVNFGALSGFMLLHLTVINYYWRRQKSGQVIRHLICPVIGFIIVAAIMYNMGVDAQKLG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LIWIALGLVYLFFLNKLGASTALPDPSNG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MEIEEFGYKQELKRSLTLTDLVVYGMIFMIPIAPFGVYGYVNAEAPGMVPLAYIIGMVAM
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHH
VFTALSYGSMARAFPIAGSVYSYAQRGLNQHVGFIAGWLMLLDYLLIPPLLYVYAAMALN
HHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLYPDIPKVGFILAFLVSATFVNLRGITFTARMNIVFLLAQLVVLGIFLFYAWNALHNGG
HHCCCCCHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GNGQLTLAPLYHPETFNFALLMQAVSIAVLSFLGFDAISTLAEEIKGDPGRSVGKAALIT
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LVVMGVIFVAQTWIATDLAAGMGFKSADTAFYEIAEIAAGSWLATLTGVATALAWGVAVA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ITSQAAVSRLLFGMARDGKLPKVLAKVHPKHNTPYLSIYLVAVLSLVICYLFINSVDTLT
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SLVNFGALSGFMLLHLTVINYYWRRQKSGQVIRHLICPVIGFIIVAAIMYNMGVDAQKLG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LIWIALGLVYLFFLNKLGASTALPDPSNG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]