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The map label for this gene is lip2 [H]

Identifier: 77458196

GI number: 77458196

Start: 2245427

End: 2246443

Strand: Reverse

Name: lip2 [H]

Synonym: Pfl01_1969

Alternate gene names: 77458196

Gene position: 2246443-2245427 (Counterclockwise)

Preceding gene: 77458198

Following gene: 77458195

Centisome position: 34.89

GC content: 64.7

Gene sequence:

>1017_bases
ATGAACATCAAGAAAACCCTCGCCGCTTCCCTGCTCGCCCTGTCCATCGGCAACGCCTTCGCCGCCGAAAGCACTGGCGT
CGAACACAACACTCAGGCCTTCCTCGACGCCCTCGCCGCCGGCGGTGGCAAACCGCTCGAGCAACTGAGCCCGAAAGACG
CCCGTGCGGTGCTGACCGGCGCCCAGGCTTCGGTGAAGGTTGACCTGTCCGGCGTTGAAGTCAGCGACAAGGCGATCAAG
GTCGACGGCCAGACGATCAACCTGAAGGTGGTGCGTCCAGCCAAGGTCAAAGGCGAGTTGCCGGTGTTCATGTTCTTCCA
CGGTGGCGGCTGGGTGTTGGGCGACTTCCCGACGCACCAACGCCTGATTCGTGATCTGGTGGTGGGCTCTGGCGCGGTGG
CGGTGTACGTCGATTACACGCCGTCGCCGGAAGCGCAGTACCCGACCGCGATCAATCAGGCTTACGCCGCGACGAAATGG
GTGGCCGAGCACGGCAAGGACATCGGTGTCGACGGCAAGCGTCTGGCGGTGGCCGGCAACAGCGTCGGCGGCAACATGGC
GGCGGTCGTGGCGTTGATGGCCAAGGAACAGAAAACCCCGGCCCTGCGCTTCCAGTTGCTGATGTGGCCGGTGACCAACG
CCGGGTTCGATAACGGCTCGTACCAGCAATTCGCCGAGGGCCACTTCCTGACCAAGGGCATGATGCAGTGGTTCTGGGAC
AACTACACCACCAACCCGGCCGAGCGTGCGCAGATCCATGCCTCGCCGCTCAACGCCAGCGCCGAACAGCTCAAGGGCCT
GCCCGCCGCGCTGGTGCAGACCGCCGAGTTCGACGTGCTGCGTGACGAAGGCGAAGGCTACGCCCGCCACCTCGACGCGG
CTGGCGTGCCGGTGACCTCGGTGCGCTACAACGGGATGATTCACGACTTCGGCCTGCTCAACCCGCTGAACGGTATTCCT
GAGGTGAAGGCCGCCGTGCGCCAGGCTGCGGCAGAACTCAAGACCCACCTGAACTGA

Upstream 100 bases:

>100_bases
TAATCTGTGCTCACGGTGTTGCTGAGGCCTGAAGCCCAGCGCCACAGCAGCCCGATCCCAAGGCATACAAAAACTCAAAC
GACTTAAAAAGGAATTGCAC

Downstream 100 bases:

>100_bases
GTCCAGCCACTCGCCACACCTTCGGGTGTGGCGTTTTCTTTTGTGTCCGCCGGAGTTCACCTCATGAGCCTTTTTTCTGC
CCACCTGCTGTCCTGGAGCG

Product: esterase/lipase/thioesterase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MNIKKTLAASLLALSIGNAFAAESTGVEHNTQAFLDALAAGGGKPLEQLSPKDARAVLTGAQASVKVDLSGVEVSDKAIK
VDGQTINLKVVRPAKVKGELPVFMFFHGGGWVLGDFPTHQRLIRDLVVGSGAVAVYVDYTPSPEAQYPTAINQAYAATKW
VAEHGKDIGVDGKRLAVAGNSVGGNMAAVVALMAKEQKTPALRFQLLMWPVTNAGFDNGSYQQFAEGHFLTKGMMQWFWD
NYTTNPAERAQIHASPLNASAEQLKGLPAALVQTAEFDVLRDEGEGYARHLDAAGVPVTSVRYNGMIHDFGLLNPLNGIP
EVKAAVRQAAAELKTHLN

Sequences:

>Translated_338_residues
MNIKKTLAASLLALSIGNAFAAESTGVEHNTQAFLDALAAGGGKPLEQLSPKDARAVLTGAQASVKVDLSGVEVSDKAIK
VDGQTINLKVVRPAKVKGELPVFMFFHGGGWVLGDFPTHQRLIRDLVVGSGAVAVYVDYTPSPEAQYPTAINQAYAATKW
VAEHGKDIGVDGKRLAVAGNSVGGNMAAVVALMAKEQKTPALRFQLLMWPVTNAGFDNGSYQQFAEGHFLTKGMMQWFWD
NYTTNPAERAQIHASPLNASAEQLKGLPAALVQTAEFDVLRDEGEGYARHLDAAGVPVTSVRYNGMIHDFGLLNPLNGIP
EVKAAVRQAAAELKTHLN
>Mature_338_residues
MNIKKTLAASLLALSIGNAFAAESTGVEHNTQAFLDALAAGGGKPLEQLSPKDARAVLTGAQASVKVDLSGVEVSDKAIK
VDGQTINLKVVRPAKVKGELPVFMFFHGGGWVLGDFPTHQRLIRDLVVGSGAVAVYVDYTPSPEAQYPTAINQAYAATKW
VAEHGKDIGVDGKRLAVAGNSVGGNMAAVVALMAKEQKTPALRFQLLMWPVTNAGFDNGSYQQFAEGHFLTKGMMQWFWD
NYTTNPAERAQIHASPLNASAEQLKGLPAALVQTAEFDVLRDEGEGYARHLDAAGVPVTSVRYNGMIHDFGLLNPLNGIP
EVKAAVRQAAAELKTHLN

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=290, Percent_Identity=26.551724137931, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI21328446, Length=116, Percent_Identity=32.7586206896552, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1786682, Length=212, Percent_Identity=27.8301886792453, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17567059, Length=269, Percent_Identity=27.1375464684015, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71996133, Length=124, Percent_Identity=31.4516129032258, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24656084, Length=98, Percent_Identity=34.6938775510204, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24656076, Length=98, Percent_Identity=34.6938775510204, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI20130169, Length=98, Percent_Identity=34.6938775510204, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 36014; Mature: 36014

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKKTLAASLLALSIGNAFAAESTGVEHNTQAFLDALAAGGGKPLEQLSPKDARAVLTG
CCHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCHHEEEEC
AQASVKVDLSGVEVSDKAIKVDGQTINLKVVRPAKVKGELPVFMFFHGGGWVLGDFPTHQ
CCEEEEEEECCCEECCCEEEECCCEEEEEEECCCCCCCCCCEEEEEECCCEEECCCCHHH
RLIRDLVVGSGAVAVYVDYTPSPEAQYPTAINQAYAATKWVAEHGKDIGVDGKRLAVAGN
HHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
SVGGNMAAVVALMAKEQKTPALRFQLLMWPVTNAGFDNGSYQQFAEGHFLTKGMMQWFWD
CCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHC
NYTTNPAERAQIHASPLNASAEQLKGLPAALVQTAEFDVLRDEGEGYARHLDAAGVPVTS
CCCCCCHHHCEEECCCCCCCHHHHCCCHHHHHHHHHHEEEECCCCCHHHHCCCCCCCEEE
VRYNGMIHDFGLLNPLNGIPEVKAAVRQAAAELKTHLN
EEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIKKTLAASLLALSIGNAFAAESTGVEHNTQAFLDALAAGGGKPLEQLSPKDARAVLTG
CCHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCHHEEEEC
AQASVKVDLSGVEVSDKAIKVDGQTINLKVVRPAKVKGELPVFMFFHGGGWVLGDFPTHQ
CCEEEEEEECCCEECCCEEEECCCEEEEEEECCCCCCCCCCEEEEEECCCEEECCCCHHH
RLIRDLVVGSGAVAVYVDYTPSPEAQYPTAINQAYAATKWVAEHGKDIGVDGKRLAVAGN
HHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
SVGGNMAAVVALMAKEQKTPALRFQLLMWPVTNAGFDNGSYQQFAEGHFLTKGMMQWFWD
CCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHC
NYTTNPAERAQIHASPLNASAEQLKGLPAALVQTAEFDVLRDEGEGYARHLDAAGVPVTS
CCCCCCHHHCEEECCCCCCCHHHHCCCHHHHHHHHHHEEEECCCCCHHHHCCCCCCCEEE
VRYNGMIHDFGLLNPLNGIPEVKAAVRQAAAELKTHLN
EEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]