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The map label for this gene is rihA [H]

Identifier: 77458152

GI number: 77458152

Start: 2197021

End: 2198049

Strand: Direct

Name: rihA [H]

Synonym: Pfl01_1925

Alternate gene names: 77458152

Gene position: 2197021-2198049 (Clockwise)

Preceding gene: 77458151

Following gene: 77458154

Centisome position: 34.12

GC content: 58.89

Gene sequence:

>1029_bases
ATGCACCGCTATGCTCAAAAATTGCATCAATTGATTCGGGGGCTGCTGCTTTTGTCCGTGATAACCGCGACCGGCGCCCA
GGCGGCGGAAAAGATCGACCTGATCATTGACACCGATCCGGGTGCCGACGACGTGGTCGCCCTGTTGTTTGCCCTGGCCT
CCCCGGAAGAGCTGAACATTCGCGCGTTGACCACCGTGGCCGGCAATGTGCGTCTGGACAAGACTTCGCGCAATGCCAGG
TTGGCCCGTGAGTGGGCTGGGCGGGAAGATATTCCGGTGTACGCCGGGGCGCCAAAACCGCTGATGCGCACGCCGATCTA
CGCTGAAAACATCCATGGCAAGGAAGGCCTGTCGGGTGTCACCGTGCATGAGCCGAAGAAAGGCCTGGCCGAAGGCAATG
CGGTCAACTATCTGATCGACACCCTGAAAAAAGCCAAGCCGCACAGCATCACCATCGCGATGCTCGGCCCGCAGACCAAC
CTGGCCCTGGCGCTGATCCAGGAGCCGGAGATCGTTCAGGGCATCAAGGAAGTCGTGATCATGGGGGGCGCTCACTTCAA
TGGCGGCAACATCACGCCAGTGGCCGAATTTAACTTGTACGCCGACCCGCAAGCCGCGGAAGTTGTGCTCAAAAGCGGCG
TGAAACTGACTTATCTGCCGCTGGACGTGACCCACAAGATCCTCACCAGTGACGCACGCCTGAAGCAGATTGCGGCGCTG
AACAACAATGCGAGCAAGATTGTCGGCGACATCCTCAACGAGTACATCAAAGGGGATATGGAGCACTACGGCATTCCGGG
TGGCCCGGTGCATGACGCCACCGTCGTGGCTTATCTGCTCAAGCCTGAACTCTTCACGGGTCGTTCGGTGAATGTGGTGG
TCGATAGCCGTGAAGGCCCGACATTCGGCCAGACCATTGTCGACTGGTATGACGGCCTGAAGGCGCCGAAGAACGCTTTC
TGGGTGGAGAACGGCGATGCCCAAGGCTTCTTCGATCTGCTGACCGAGCGTCTGAAGCGTCTGAAGTAA

Upstream 100 bases:

>100_bases
CGGGAGAATGCCAGCCGTACTGCAACATCGTGCTGGTGGCTGGGGTTACGTTCTAAACAGGGTTCGCCCCTTTTGATTTC
ATCTGCACAAGGAATGCGCC

Downstream 100 bases:

>100_bases
GCCATACGCCCGCAAGCGCCAGCTTGCGGGCTTTTACTTTCTCTCGGTGTCCGATGTCCCTCCGAAGTCATCGGGATACT
TCTCGAGCAGCTGGGCAATG

Product: inosine/uridine-preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MHRYAQKLHQLIRGLLLLSVITATGAQAAEKIDLIIDTDPGADDVVALLFALASPEELNIRALTTVAGNVRLDKTSRNAR
LAREWAGREDIPVYAGAPKPLMRTPIYAENIHGKEGLSGVTVHEPKKGLAEGNAVNYLIDTLKKAKPHSITIAMLGPQTN
LALALIQEPEIVQGIKEVVIMGGAHFNGGNITPVAEFNLYADPQAAEVVLKSGVKLTYLPLDVTHKILTSDARLKQIAAL
NNNASKIVGDILNEYIKGDMEHYGIPGGPVHDATVVAYLLKPELFTGRSVNVVVDSREGPTFGQTIVDWYDGLKAPKNAF
WVENGDAQGFFDLLTERLKRLK

Sequences:

>Translated_342_residues
MHRYAQKLHQLIRGLLLLSVITATGAQAAEKIDLIIDTDPGADDVVALLFALASPEELNIRALTTVAGNVRLDKTSRNAR
LAREWAGREDIPVYAGAPKPLMRTPIYAENIHGKEGLSGVTVHEPKKGLAEGNAVNYLIDTLKKAKPHSITIAMLGPQTN
LALALIQEPEIVQGIKEVVIMGGAHFNGGNITPVAEFNLYADPQAAEVVLKSGVKLTYLPLDVTHKILTSDARLKQIAAL
NNNASKIVGDILNEYIKGDMEHYGIPGGPVHDATVVAYLLKPELFTGRSVNVVVDSREGPTFGQTIVDWYDGLKAPKNAF
WVENGDAQGFFDLLTERLKRLK
>Mature_342_residues
MHRYAQKLHQLIRGLLLLSVITATGAQAAEKIDLIIDTDPGADDVVALLFALASPEELNIRALTTVAGNVRLDKTSRNAR
LAREWAGREDIPVYAGAPKPLMRTPIYAENIHGKEGLSGVTVHEPKKGLAEGNAVNYLIDTLKKAKPHSITIAMLGPQTN
LALALIQEPEIVQGIKEVVIMGGAHFNGGNITPVAEFNLYADPQAAEVVLKSGVKLTYLPLDVTHKILTSDARLKQIAAL
NNNASKIVGDILNEYIKGDMEHYGIPGGPVHDATVVAYLLKPELFTGRSVNVVVDSREGPTFGQTIVDWYDGLKAPKNAF
WVENGDAQGFFDLLTERLKRLK

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=307, Percent_Identity=42.9967426710098, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1788486, Length=311, Percent_Identity=40.5144694533762, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1786213, Length=309, Percent_Identity=36.8932038834951, Blast_Score=167, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI32565474, Length=204, Percent_Identity=38.2352941176471, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17565698, Length=308, Percent_Identity=28.8961038961039, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320608, Length=262, Percent_Identity=30.5343511450382, Blast_Score=106, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24641837, Length=322, Percent_Identity=30.7453416149068, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24641839, Length=300, Percent_Identity=29, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583915, Length=301, Percent_Identity=25.9136212624585, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 37117; Mature: 37117

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYAQKLHQLIRGLLLLSVITATGAQAAEKIDLIIDTDPGADDVVALLFALASPEELNI
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCCCE
RALTTVAGNVRLDKTSRNARLAREWAGREDIPVYAGAPKPLMRTPIYAENIHGKEGLSGV
EEEEEEECCEEEECCCCCHHHHHHHCCCCCCCEECCCCCCHHHCCCHHCCCCCCCCCCCC
TVHEPKKGLAEGNAVNYLIDTLKKAKPHSITIAMLGPQTNLALALIQEPEIVQGIKEVVI
EEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHE
MGGAHFNGGNITPVAEFNLYADPQAAEVVLKSGVKLTYLPLDVTHKILTSDARLKQIAAL
ECCCEECCCCCCEEEEEEEECCCHHHHHHHHCCCEEEEEEHHHHHHHHHCCHHHHHHHHH
NNNASKIVGDILNEYIKGDMEHYGIPGGPVHDATVVAYLLKPELFTGRSVNVVVDSREGP
CCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCC
TFGQTIVDWYDGLKAPKNAFWVENGDAQGFFDLLTERLKRLK
CHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MHRYAQKLHQLIRGLLLLSVITATGAQAAEKIDLIIDTDPGADDVVALLFALASPEELNI
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCCCE
RALTTVAGNVRLDKTSRNARLAREWAGREDIPVYAGAPKPLMRTPIYAENIHGKEGLSGV
EEEEEEECCEEEECCCCCHHHHHHHCCCCCCCEECCCCCCHHHCCCHHCCCCCCCCCCCC
TVHEPKKGLAEGNAVNYLIDTLKKAKPHSITIAMLGPQTNLALALIQEPEIVQGIKEVVI
EEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHE
MGGAHFNGGNITPVAEFNLYADPQAAEVVLKSGVKLTYLPLDVTHKILTSDARLKQIAAL
ECCCEECCCCCCEEEEEEEECCCHHHHHHHHCCCEEEEEEHHHHHHHHHCCHHHHHHHHH
NNNASKIVGDILNEYIKGDMEHYGIPGGPVHDATVVAYLLKPELFTGRSVNVVVDSREGP
CCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCC
TFGQTIVDWYDGLKAPKNAFWVENGDAQGFFDLLTERLKRLK
CHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA