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The map label for this gene is folC [H]

Identifier: 77458126

GI number: 77458126

Start: 2170079

End: 2171386

Strand: Direct

Name: folC [H]

Synonym: Pfl01_1899

Alternate gene names: 77458126

Gene position: 2170079-2171386 (Clockwise)

Preceding gene: 77458125

Following gene: 77458127

Centisome position: 33.71

GC content: 62.39

Gene sequence:

>1308_bases
ATGACCGAGCGCACCCTTGGCGAGTGGCTCGCCTACCTTGAACAGTTGCATCCATCGGCCATCGACATGGGGCTGGAGCG
TTCGCAACAGGTAGCGTCCCGCATGGGACTGGGCCAGCCGGCGCCTCGGGTGATTACGGTCACCGGCACCAACGGCAAGG
GTTCGACCTGCGCGTTCGTGGCTTCATTGCTGCGAGCGCAGGGCCTGAACGTCGGTGTCTACAATTCTCCGCACTTGCTG
CGTTACAACGAGCGGGTGCAGCTCAATGGCGTCGAAGCCACTGACGCGCAGCTGTGCGAAGCCTTCGCGGCGGTCGAAGC
GGGGCGCGGCGACACTTCCCTGACTTACTTCGAAATGGGCACCCTGGCGGCGTTCTGGCTGTTTCAACAGGCCGGGCTCG
ACGCGGTGGTGCTGGAAGTCGGGCTGGGCGGGCGTCTGGATACGGTCAACGTGGTCGATGCCGACATCGCGCTGGTCACC
AGCATTGGTGTCGATCATGCCGATTACCTGGGCAATACCCGCGAATCCGTGGCCTTCGAGAAGGCCGGGATTTTCCGTCA
GGGCAAGCCTGCGCTGTGCGGCGATCTGAATCCTCCACAACCCCTGCTGGACAAGGCGCGCGAGCTCGCTTGCCCGTTCT
TCCTGCGCGGGCGCGATTTCGATCTGGGTATCACGGATCAATTCTGGCAGTGGCGCGGTACCGATGCTGACGGTCAGGTC
GTTGAGCTGCGCGATTTGCCGCTGCTTGATCTGCCGATGGAAAACGCTGCGCTGGCGTTGCAGGCTTATCTGCTGCTCGG
TTTGCCATGGGATGCCAAACAGATTGTTGCGGCGTTGCAGGCGACTCGCGTGGTCGGTCGTCTTGATCGTCGCTCGTTCG
AATGGAACGGCAAGCGCTTGAACCTGCTGCTGGACGTTGGCCATAACCCGCATGCGGCGGAGTATCTGGCCCGGCGTCTG
GCGGCGCGTCCGCCGGTCGGCAAGCGTCTGGCGGTGTTCGGCCTGTTGGCGGACAAGGATCTGGACGGTGTCATCGGTGA
ATTGAATGCCAGTGTCCAGCATTGGGCGGTGGCGCCGCTGGATTCGCCGCGTGCGCGTCCGGTGGCTGAATTGAATGTTG
CACTGCAGAACCTTGGCGCCTCGGTCACGTCTTATGACAGTGTGGCGGCTGCGCTGGAAGAGCAGTGTGCAGTGGCCACC
AGCGACGACGAGATCTTGCTGTTCGGATCATTTTATTGTGTCGCCGAGGCCCTCGAATGGCTGTCTCGGCGCTCCACGGA
GGAAGCGGCAAATGGCTTTGCTGGATAA

Upstream 100 bases:

>100_bases
GTCTGGGTAACCTGCTGGCGCAACTGACCGGCAAACCGACGCCTAAATTCGTTGCTGCGCCGATCGAGCCGATCGTCGTT
CCGCCGGTGCCTGCCAACGT

Downstream 100 bases:

>100_bases
AGCGTACAAACAGCGCATGGTCGGTGCCTTGGTGCTGGTGGCGCTGGCGGTGATTTTCCTGCCGATGCTGTTTTCCCGTC
AGGATGAACAGCGTCAGGTG

Product: folylpolyglutamate synthetase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTERTLGEWLAYLEQLHPSAIDMGLERSQQVASRMGLGQPAPRVITVTGTNGKGSTCAFVASLLRAQGLNVGVYNSPHLL
RYNERVQLNGVEATDAQLCEAFAAVEAGRGDTSLTYFEMGTLAAFWLFQQAGLDAVVLEVGLGGRLDTVNVVDADIALVT
SIGVDHADYLGNTRESVAFEKAGIFRQGKPALCGDLNPPQPLLDKARELACPFFLRGRDFDLGITDQFWQWRGTDADGQV
VELRDLPLLDLPMENAALALQAYLLLGLPWDAKQIVAALQATRVVGRLDRRSFEWNGKRLNLLLDVGHNPHAAEYLARRL
AARPPVGKRLAVFGLLADKDLDGVIGELNASVQHWAVAPLDSPRARPVAELNVALQNLGASVTSYDSVAAALEEQCAVAT
SDDEILLFGSFYCVAEALEWLSRRSTEEAANGFAG

Sequences:

>Translated_435_residues
MTERTLGEWLAYLEQLHPSAIDMGLERSQQVASRMGLGQPAPRVITVTGTNGKGSTCAFVASLLRAQGLNVGVYNSPHLL
RYNERVQLNGVEATDAQLCEAFAAVEAGRGDTSLTYFEMGTLAAFWLFQQAGLDAVVLEVGLGGRLDTVNVVDADIALVT
SIGVDHADYLGNTRESVAFEKAGIFRQGKPALCGDLNPPQPLLDKARELACPFFLRGRDFDLGITDQFWQWRGTDADGQV
VELRDLPLLDLPMENAALALQAYLLLGLPWDAKQIVAALQATRVVGRLDRRSFEWNGKRLNLLLDVGHNPHAAEYLARRL
AARPPVGKRLAVFGLLADKDLDGVIGELNASVQHWAVAPLDSPRARPVAELNVALQNLGASVTSYDSVAAALEEQCAVAT
SDDEILLFGSFYCVAEALEWLSRRSTEEAANGFAG
>Mature_434_residues
TERTLGEWLAYLEQLHPSAIDMGLERSQQVASRMGLGQPAPRVITVTGTNGKGSTCAFVASLLRAQGLNVGVYNSPHLLR
YNERVQLNGVEATDAQLCEAFAAVEAGRGDTSLTYFEMGTLAAFWLFQQAGLDAVVLEVGLGGRLDTVNVVDADIALVTS
IGVDHADYLGNTRESVAFEKAGIFRQGKPALCGDLNPPQPLLDKARELACPFFLRGRDFDLGITDQFWQWRGTDADGQVV
ELRDLPLLDLPMENAALALQAYLLLGLPWDAKQIVAALQATRVVGRLDRRSFEWNGKRLNLLLDVGHNPHAAEYLARRLA
ARPPVGKRLAVFGLLADKDLDGVIGELNASVQHWAVAPLDSPRARPVAELNVALQNLGASVTSYDSVAAALEEQCAVATS
DDEILLFGSFYCVAEALEWLSRRSTEEAANGFAG

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=183, Percent_Identity=39.344262295082, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI66932990, Length=183, Percent_Identity=39.344262295082, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1788654, Length=421, Percent_Identity=47.0308788598575, Blast_Score=348, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI17553148, Length=192, Percent_Identity=38.0208333333333, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71984923, Length=329, Percent_Identity=31.0030395136778, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17553150, Length=322, Percent_Identity=31.3664596273292, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323760, Length=325, Percent_Identity=28.3076923076923, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6324815, Length=212, Percent_Identity=34.4339622641509, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24641571, Length=161, Percent_Identity=37.888198757764, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24581568, Length=146, Percent_Identity=36.986301369863, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 47070; Mature: 46939

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERTLGEWLAYLEQLHPSAIDMGLERSQQVASRMGLGQPAPRVITVTGTNGKGSTCAFV
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHH
ASLLRAQGLNVGVYNSPHLLRYNERVQLNGVEATDAQLCEAFAAVEAGRGDTSLTYFEMG
HHHHHHCCCCEEECCCCCEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHH
TLAAFWLFQQAGLDAVVLEVGLGGRLDTVNVVDADIALVTSIGVDHADYLGNTRESVAFE
HHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCHHHHHCCCCHHHHCCCCHHHHHHH
KAGIFRQGKPALCGDLNPPQPLLDKARELACPFFLRGRDFDLGITDQFWQWRGTDADGQV
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCHHHHEECCCCCCCCE
VELRDLPLLDLPMENAALALQAYLLLGLPWDAKQIVAALQATRVVGRLDRRSFEWNGKRL
EEEECCCEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
NLLLDVGHNPHAAEYLARRLAARPPVGKRLAVFGLLADKDLDGVIGELNASVQHWAVAPL
EEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCCHHHEEECCC
DSPRARPVAELNVALQNLGASVTSYDSVAAALEEQCAVATSDDEILLFGSFYCVAEALEW
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEECCCCCEEEEEHHHHHHHHHHH
LSRRSTEEAANGFAG
HHHCCHHHHHCCCCC
>Mature Secondary Structure 
TERTLGEWLAYLEQLHPSAIDMGLERSQQVASRMGLGQPAPRVITVTGTNGKGSTCAFV
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHH
ASLLRAQGLNVGVYNSPHLLRYNERVQLNGVEATDAQLCEAFAAVEAGRGDTSLTYFEMG
HHHHHHCCCCEEECCCCCEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHH
TLAAFWLFQQAGLDAVVLEVGLGGRLDTVNVVDADIALVTSIGVDHADYLGNTRESVAFE
HHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCHHHHHCCCCHHHHCCCCHHHHHHH
KAGIFRQGKPALCGDLNPPQPLLDKARELACPFFLRGRDFDLGITDQFWQWRGTDADGQV
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCHHHHEECCCCCCCCE
VELRDLPLLDLPMENAALALQAYLLLGLPWDAKQIVAALQATRVVGRLDRRSFEWNGKRL
EEEECCCEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
NLLLDVGHNPHAAEYLARRLAARPPVGKRLAVFGLLADKDLDGVIGELNASVQHWAVAPL
EEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCCHHHEEECCC
DSPRARPVAELNVALQNLGASVTSYDSVAAALEEQCAVATSDDEILLFGSFYCVAEALEW
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEECCCCCEEEEEHHHHHHHHHHH
LSRRSTEEAANGFAG
HHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]