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The map label for this gene is leuD [H]

Identifier: 77458117

GI number: 77458117

Start: 2159552

End: 2160196

Strand: Direct

Name: leuD [H]

Synonym: Pfl01_1890

Alternate gene names: 77458117

Gene position: 2159552-2160196 (Clockwise)

Preceding gene: 77458116

Following gene: 77458118

Centisome position: 33.54

GC content: 58.91

Gene sequence:

>645_bases
ATGAAAGCTTTTACCCAGCACACTGGTCTCGTCGCGCCTCTGGATCGTGCCAACGTCGACACCGACCAGATCATTCCGAA
GCAGTTTCTGAAGTCGATCAAGCGCACCGGTTTCGGTCCGAACCTGTTCGACGAGTGGCGTTACCTGGATGTGGGTCAGC
CGTATCAGGACAACTCCAAGCGTCCGCTGAACAAGGAATTCGTACTCAACGCCGAGCGTTATCAAGGCGCCAGCGTGTTG
CTGGCCCGCGAAAACTTCGGCTGCGGCTCCAGCCGTGAACACGCGCCGTGGGCGCTGGAAGAATACGGTTTCCGCAGCAT
CATCGCGCCGAGCTATGCCGACATTTTCTTCAACAACAGCTTCAAGAACGGCTTGCTTCCGATCATCTTGAGCGACGCCG
AAGTCGACGAGCTGTTCAAACAGGTCGAGGCCGCGCCGGGCTATCAGTTGCAGATCGATCTGCACGAGCAGACCGTGACC
AGCCCGAACGGCAAGGTCTATCGCTTCGAGATCGACGCCTTCCGCAAACACTGCCTGCTCAATGGTCTGGACGACATCGG
CCTGACCCTGCAGGACGGCGATGCGATTGCCGCGTTTGAAGCCAGGCATCGCGCGAGCCAGCCATGGTTGTTCCGCGACG
CGTGA

Upstream 100 bases:

>100_bases
CCGGTGGCCGTACCCACCTCGTCAGCCCGGCCATGGCCGCTGCGGCGGCGGTGAACGGTCGTTTCATCGACGTTCGTGAA
TTGATCTAAAGGAGCGCAGC

Downstream 100 bases:

>100_bases
ATCTGAAAGGTTGAAACCCAGAAAAGGACGTACCCATGACCAGCACCGCCCAGCACAGTCAGGTTGTACAAAAGCAATTC
GGTGAACAGGCCGCCGCCTA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKAFTQHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPYQDNSKRPLNKEFVLNAERYQGASVL
LARENFGCGSSREHAPWALEEYGFRSIIAPSYADIFFNNSFKNGLLPIILSDAEVDELFKQVEAAPGYQLQIDLHEQTVT
SPNGKVYRFEIDAFRKHCLLNGLDDIGLTLQDGDAIAAFEARHRASQPWLFRDA

Sequences:

>Translated_214_residues
MKAFTQHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPYQDNSKRPLNKEFVLNAERYQGASVL
LARENFGCGSSREHAPWALEEYGFRSIIAPSYADIFFNNSFKNGLLPIILSDAEVDELFKQVEAAPGYQLQIDLHEQTVT
SPNGKVYRFEIDAFRKHCLLNGLDDIGLTLQDGDAIAAFEARHRASQPWLFRDA
>Mature_214_residues
MKAFTQHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPYQDNSKRPLNKEFVLNAERYQGASVL
LARENFGCGSSREHAPWALEEYGFRSIIAPSYADIFFNNSFKNGLLPIILSDAEVDELFKQVEAAPGYQLQIDLHEQTVT
SPNGKVYRFEIDAFRKHCLLNGLDDIGLTLQDGDAIAAFEARHRASQPWLFRDA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=202, Percent_Identity=63.8613861386139, Blast_Score=264, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6321429, Length=213, Percent_Identity=51.6431924882629, Blast_Score=208, Evalue=5e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 24269; Mature: 24269

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFTQHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPYQDNSK
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCCCCCC
RPLNKEFVLNAERYQGASVLLARENFGCGSSREHAPWALEEYGFRSIIAPSYADIFFNNS
CCCCHHEEEEHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCHHHCCCCHHHEEECCC
FKNGLLPIILSDAEVDELFKQVEAAPGYQLQIDLHEQTVTSPNGKVYRFEIDAFRKHCLL
CCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHEECCCCCEEEEEEHHHHHHHHHH
NGLDDIGLTLQDGDAIAAFEARHRASQPWLFRDA
CCCCCCCEEEECCCEEEEHHHHHCCCCCEEEECC
>Mature Secondary Structure
MKAFTQHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPYQDNSK
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCCCCCC
RPLNKEFVLNAERYQGASVLLARENFGCGSSREHAPWALEEYGFRSIIAPSYADIFFNNS
CCCCHHEEEEHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCHHHCCCCHHHEEECCC
FKNGLLPIILSDAEVDELFKQVEAAPGYQLQIDLHEQTVTSPNGKVYRFEIDAFRKHCLL
CCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHEECCCCCEEEEEEHHHHHHHHHH
NGLDDIGLTLQDGDAIAAFEARHRASQPWLFRDA
CCCCCCCEEEECCCEEEEHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA