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The map label for this gene is gltX [H]

Identifier: 77458105

GI number: 77458105

Start: 2142449

End: 2143993

Strand: Direct

Name: gltX [H]

Synonym: Pfl01_1878

Alternate gene names: 77458105

Gene position: 2142449-2143993 (Clockwise)

Preceding gene: 77458102

Following gene: 77458106

Centisome position: 33.28

GC content: 60.52

Gene sequence:

>1545_bases
GTGTCGCCTGTTACCATTCGCCGCTTGATCGTTCTTTGCTTTTCATTCTTTTCGAGACATGCCATGACCACCGTCCGCAC
TCGCATCGCGCCATCGCCTACCGGGGATCCCCACGTAGGTACCGCTTACATCGCACTGTTCAACTACTGCTTTGCCAAGC
AGCACGGCGGTGAGTTCATCCTGCGGATCGAAGACACCGATCAATTGCGTTCGACCCGCGAGTCCGAACAGCAGATCTTC
GACGCCCTGCGCTGGCTGGGTATCGACTGGAGCGAAGGCCCGGACGTCGGCGGCCCGCACGGCCCGTACCGTCAGAGCGA
GCGCGGCGATATCTATCAGAAGTATTGCCAGCAACTGGTCGACATGGGCCACGCCTTCCCGTGCTTCTGCACCGCTGAAG
AGCTGGACCAGATGCGCGCCGAGCAAATGGCGCGCGGCGAAACCCCGCGTTACGACGGCCGTGCACTGTTGCTGTCCAAG
GAAGAAGTCGCCCGGCGCCTGGCTGCCGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGCGTCTGCGTGGT
GCCGGACATGCTGCGTGGCGATGTCGAGATCCCGTGGGATCGCATGGACATGCAAGTGCTGATGAAGACCGACGGCCTGC
CGACGTACTTCCTGGCCAACGTGGTCGACGATCACCTGATGGGCATCACTCACGTGCTGCGCGGCGAAGAATGGTTGCCG
TCGGCGCCGAAACTGATCCTGCTTTACGAGTATTTCGGCTGGGAACAACCGGAGCTGTGCTACATGCCGCTGCTGCGTAA
CCCGGACAAGAGCAAGCTGTCCAAGCGCAAGAACCCGACTTCGGTGACGTTCTACGAGCGCATGGGTTTCATGCCTGAGG
CGATGCTTAACTACCTGGGCCGCATGGGCTGGTCGATGCCGGACGAGCGCGAGAAGTTCTCGCTGCAGGAAATGGTCGAC
AACTTCGACCTCAAGCGCGTGTCGCTGGGTGGGCCGATTTTCGATATCGAGAAGCTGTCGTGGCTCAACGGCCAGTGGCT
GCGTGATCTGCCGGTGGAAGAGTTCGCCGCTCGCGTTCAGAAGTGGGCGTTCAACTCCGAGTACATGATGAAGATCGCGC
CGCATGTTCAGGGCCGTGTCGAAACCTTCAGCCAGGTTGCACCGCTGGCCGGGTTCTTCTTCGCCGGTGGCGTGAACCCG
GATGCCAAGCTGTTCGAATCGAAAAAGCTTTCGGGCGATCAGGTTCGTCAGTTGATGCAATTGATCCTGTGGAAGCTGGA
AAGCCTGCGTCAGTGGGAGAAGGACAGCATCACCGCGACGATTCAGGCGGTGGTCGAATCCCTCGAGCTCAAGCTGCGTG
ATGCGATGCCGCTGATGTTTGCCGCGATTACCGGGCAGGCGAGTTCGGTGTCGGTGCTCGATGCGATGGAAATCCTCGGG
CCGGACCTGACCCGTTTCCGTCTGCGCCAGGCGATTGACCTGCTGGGCGGCGTGTCGAAGAAAGAAAACAAAGAGTGGGA
AAAGCTGTTGGGCGCCATCGCCTGA

Upstream 100 bases:

>100_bases
ACGGTAAATGCCGTCTGTTGATATTCGGCCATTCACCACGGCCAACGGGCGTTCCAGACGCAACCTTGGCTTAAATGCCC
TGCCCGCTGGAGCGGGGCGG

Downstream 100 bases:

>100_bases
TTGCGTTTGACTCTGCAGGACGCGCCGAAGGTCAGGATCTTTTGATCCTGTCCTTCGGTGTTTTCCCCCGAATTTACGGG
GGGAGGGCGGTAAGTGATTG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSPVTIRRLIVLCFSFFSRHAMTTVRTRIAPSPTGDPHVGTAYIALFNYCFAKQHGGEFILRIEDTDQLRSTRESEQQIF
DALRWLGIDWSEGPDVGGPHGPYRQSERGDIYQKYCQQLVDMGHAFPCFCTAEELDQMRAEQMARGETPRYDGRALLLSK
EEVARRLAAGEPHVIRMKVPSEGVCVVPDMLRGDVEIPWDRMDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWLP
SAPKLILLYEYFGWEQPELCYMPLLRNPDKSKLSKRKNPTSVTFYERMGFMPEAMLNYLGRMGWSMPDEREKFSLQEMVD
NFDLKRVSLGGPIFDIEKLSWLNGQWLRDLPVEEFAARVQKWAFNSEYMMKIAPHVQGRVETFSQVAPLAGFFFAGGVNP
DAKLFESKKLSGDQVRQLMQLILWKLESLRQWEKDSITATIQAVVESLELKLRDAMPLMFAAITGQASSVSVLDAMEILG
PDLTRFRLRQAIDLLGGVSKKENKEWEKLLGAIA

Sequences:

>Translated_514_residues
MSPVTIRRLIVLCFSFFSRHAMTTVRTRIAPSPTGDPHVGTAYIALFNYCFAKQHGGEFILRIEDTDQLRSTRESEQQIF
DALRWLGIDWSEGPDVGGPHGPYRQSERGDIYQKYCQQLVDMGHAFPCFCTAEELDQMRAEQMARGETPRYDGRALLLSK
EEVARRLAAGEPHVIRMKVPSEGVCVVPDMLRGDVEIPWDRMDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWLP
SAPKLILLYEYFGWEQPELCYMPLLRNPDKSKLSKRKNPTSVTFYERMGFMPEAMLNYLGRMGWSMPDEREKFSLQEMVD
NFDLKRVSLGGPIFDIEKLSWLNGQWLRDLPVEEFAARVQKWAFNSEYMMKIAPHVQGRVETFSQVAPLAGFFFAGGVNP
DAKLFESKKLSGDQVRQLMQLILWKLESLRQWEKDSITATIQAVVESLELKLRDAMPLMFAAITGQASSVSVLDAMEILG
PDLTRFRLRQAIDLLGGVSKKENKEWEKLLGAIA
>Mature_513_residues
SPVTIRRLIVLCFSFFSRHAMTTVRTRIAPSPTGDPHVGTAYIALFNYCFAKQHGGEFILRIEDTDQLRSTRESEQQIFD
ALRWLGIDWSEGPDVGGPHGPYRQSERGDIYQKYCQQLVDMGHAFPCFCTAEELDQMRAEQMARGETPRYDGRALLLSKE
EVARRLAAGEPHVIRMKVPSEGVCVVPDMLRGDVEIPWDRMDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWLPS
APKLILLYEYFGWEQPELCYMPLLRNPDKSKLSKRKNPTSVTFYERMGFMPEAMLNYLGRMGWSMPDEREKFSLQEMVDN
FDLKRVSLGGPIFDIEKLSWLNGQWLRDLPVEEFAARVQKWAFNSEYMMKIAPHVQGRVETFSQVAPLAGFFFAGGVNPD
AKLFESKKLSGDQVRQLMQLILWKLESLRQWEKDSITATIQAVVESLELKLRDAMPLMFAAITGQASSVSVLDAMEILGP
DLTRFRLRQAIDLLGGVSKKENKEWEKLLGAIA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=500, Percent_Identity=34.6, Blast_Score=278, Evalue=8e-75,
Organism=Escherichia coli, GI1788743, Length=490, Percent_Identity=35.7142857142857, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI226510925, Length=269, Percent_Identity=32.3420074349442, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71993394, Length=482, Percent_Identity=32.7800829875519, Blast_Score=261, Evalue=8e-70,
Organism=Saccharomyces cerevisiae, GI6324540, Length=506, Percent_Identity=33.399209486166, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6321192, Length=223, Percent_Identity=27.3542600896861, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324742, Length=225, Percent_Identity=24.8888888888889, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665321, Length=513, Percent_Identity=34.307992202729, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 58708; Mature: 58576

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVTIRRLIVLCFSFFSRHAMTTVRTRIAPSPTGDPHVGTAYIALFNYCFAKQHGGEFI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
LRIEDTDQLRSTRESEQQIFDALRWLGIDWSEGPDVGGPHGPYRQSERGDIYQKYCQQLV
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
DMGHAFPCFCTAEELDQMRAEQMARGETPRYDGRALLLSKEEVARRLAAGEPHVIRMKVP
HCCCCCCCEECHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHCCCCCEEEEECC
SEGVCVVPDMLRGDVEIPWDRMDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWLP
CCCEEECHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
SAPKLILLYEYFGWEQPELCYMPLLRNPDKSKLSKRKNPTSVTFYERMGFMPEAMLNYLG
CCCCEEEEEHHHCCCCCCEEECHHHCCCCHHHHHHCCCCCEEEHHHHHCCCHHHHHHHHH
RMGWSMPDEREKFSLQEMVDNFDLKRVSLGGPIFDIEKLSWLNGQWLRDLPVEEFAARVQ
HCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHCCCCHHHCCCHHHHHHHHH
KWAFNSEYMMKIAPHVQGRVETFSQVAPLAGFFFAGGVNPDAKLFESKKLSGDQVRQLMQ
HHCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHH
LILWKLESLRQWEKDSITATIQAVVESLELKLRDAMPLMFAAITGQASSVSVLDAMEILG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
PDLTRFRLRQAIDLLGGVSKKENKEWEKLLGAIA
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SPVTIRRLIVLCFSFFSRHAMTTVRTRIAPSPTGDPHVGTAYIALFNYCFAKQHGGEFI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
LRIEDTDQLRSTRESEQQIFDALRWLGIDWSEGPDVGGPHGPYRQSERGDIYQKYCQQLV
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
DMGHAFPCFCTAEELDQMRAEQMARGETPRYDGRALLLSKEEVARRLAAGEPHVIRMKVP
HCCCCCCCEECHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHCCCCCEEEEECC
SEGVCVVPDMLRGDVEIPWDRMDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWLP
CCCEEECHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
SAPKLILLYEYFGWEQPELCYMPLLRNPDKSKLSKRKNPTSVTFYERMGFMPEAMLNYLG
CCCCEEEEEHHHCCCCCCEEECHHHCCCCHHHHHHCCCCCEEEHHHHHCCCHHHHHHHHH
RMGWSMPDEREKFSLQEMVDNFDLKRVSLGGPIFDIEKLSWLNGQWLRDLPVEEFAARVQ
HCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHCCCCHHHCCCHHHHHHHHH
KWAFNSEYMMKIAPHVQGRVETFSQVAPLAGFFFAGGVNPDAKLFESKKLSGDQVRQLMQ
HHCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHH
LILWKLESLRQWEKDSITATIQAVVESLELKLRDAMPLMFAAITGQASSVSVLDAMEILG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
PDLTRFRLRQAIDLLGGVSKKENKEWEKLLGAIA
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA