| Definition | Pseudomonas fluorescens Pf0-1 chromosome, complete genome. |
|---|---|
| Accession | NC_007492 |
| Length | 6,438,405 |
Click here to switch to the map view.
The map label for this gene is dkgB [H]
Identifier: 77458092
GI number: 77458092
Start: 2127643
End: 2128446
Strand: Reverse
Name: dkgB [H]
Synonym: Pfl01_1865
Alternate gene names: 77458092
Gene position: 2128446-2127643 (Counterclockwise)
Preceding gene: 77458097
Following gene: 77458084
Centisome position: 33.06
GC content: 60.32
Gene sequence:
>804_bases ATGTCTGTTCCCGCTTTCGGTCTTGGTACGTTTCGCCTGCAAGGTCAGGTGGTCATCGATTCGGTGAGCACTGCCCTTGA GCTCGGTTACCGGGTCATCGACACCGCCCAGATCTACGAAAACGAAGCCGATGTCGGCGAGGCCATCGCTGCCAGCGGTA TTGCCCGCGACAAACTGTTCATCACCAGCAAGATCTGGATCGCCAACTTCGCCAAGGATCGTCTGATCGAAAGCCTCAAG GCGAGCCTGCAAAAACTGCAGACCGACTACCTTGACCTGACGCTGATCCACTGGCCGTCGCCGGAAGATCAGGTGCCCGT CGAAGAGTTCATGGGCGCGCTGCTGGAAGCCAAACGCCTGGGCCTGACCCGGCAGATCGGCGTGTCCAACTTCACCATCG ACCTGATGAAACAGGCGATTGCTGCGGTCGGCGCAGAAAATATCGCCACCAACCAGATCGAACTCCACCCGTACCTGCAA AACCGCAAGGTCGTCGAGTTCGCGCAAAGTCAGGGCATCCAGATCACGTCATACATGACCCTGGCCTATGGCGAAGTGCT GAAGGATCCGCTGATCGTGCAGATCGCCGAGCGTCTGCAAGCCACCCCGGCGCAGGTCACTCTGGCGTGGGCGATGCAAT CGGGTTACGCGGTGATTCCGTCGTCGACCAAACGTGCCAACCTGCAAGGCAACCTTGGCGCCATGGCGCTGACCCTGAGC GACGCCGACATGGCGCTGATCGCCACACTGGATCGCGGCCATCGCCTGACCAGCCCCAAAGGCATCGCGCCGCAGTGGGA CTGA
Upstream 100 bases:
>100_bases TGCTTTTCAGTCAACAGTACTTTGCCCCGACAGCGGTTTTTCCCGAGGAATCAGACGCGGATACTCCTGCCCATACCCAC TGCAACCCTGGAGTTCCTGC
Downstream 100 bases:
>100_bases CCGATCAGGCCTGAAGCTGCTGCGCGAGGTCGTGCATGGCCACGCGCAGCGAATCGATGGCGGCCGTCAATGCCGCTGCG GGCTGATCGTCACGGCAAGC
Product: 2,5-diketo-D-gluconate reductase B
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 267; Mature: 266
Protein sequence:
>267_residues MSVPAFGLGTFRLQGQVVIDSVSTALELGYRVIDTAQIYENEADVGEAIAASGIARDKLFITSKIWIANFAKDRLIESLK ASLQKLQTDYLDLTLIHWPSPEDQVPVEEFMGALLEAKRLGLTRQIGVSNFTIDLMKQAIAAVGAENIATNQIELHPYLQ NRKVVEFAQSQGIQITSYMTLAYGEVLKDPLIVQIAERLQATPAQVTLAWAMQSGYAVIPSSTKRANLQGNLGAMALTLS DADMALIATLDRGHRLTSPKGIAPQWD
Sequences:
>Translated_267_residues MSVPAFGLGTFRLQGQVVIDSVSTALELGYRVIDTAQIYENEADVGEAIAASGIARDKLFITSKIWIANFAKDRLIESLK ASLQKLQTDYLDLTLIHWPSPEDQVPVEEFMGALLEAKRLGLTRQIGVSNFTIDLMKQAIAAVGAENIATNQIELHPYLQ NRKVVEFAQSQGIQITSYMTLAYGEVLKDPLIVQIAERLQATPAQVTLAWAMQSGYAVIPSSTKRANLQGNLGAMALTLS DADMALIATLDRGHRLTSPKGIAPQWD >Mature_266_residues SVPAFGLGTFRLQGQVVIDSVSTALELGYRVIDTAQIYENEADVGEAIAASGIARDKLFITSKIWIANFAKDRLIESLKA SLQKLQTDYLDLTLIHWPSPEDQVPVEEFMGALLEAKRLGLTRQIGVSNFTIDLMKQAIAAVGAENIATNQIELHPYLQN RKVVEFAQSQGIQITSYMTLAYGEVLKDPLIVQIAERLQATPAQVTLAWAMQSGYAVIPSSTKRANLQGNLGAMALTLSD ADMALIATLDRGHRLTSPKGIAPQWD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI223468663, Length=286, Percent_Identity=32.8671328671329, Blast_Score=152, Evalue=2e-37, Organism=Homo sapiens, GI291291012, Length=270, Percent_Identity=32.2222222222222, Blast_Score=140, Evalue=9e-34, Organism=Homo sapiens, GI24497577, Length=290, Percent_Identity=29.6551724137931, Blast_Score=140, Evalue=2e-33, Organism=Homo sapiens, GI5174391, Length=290, Percent_Identity=29.6551724137931, Blast_Score=140, Evalue=2e-33, Organism=Homo sapiens, GI93277124, Length=299, Percent_Identity=29.4314381270903, Blast_Score=139, Evalue=2e-33, Organism=Homo sapiens, GI310109922, Length=272, Percent_Identity=30.8823529411765, Blast_Score=139, Evalue=2e-33, Organism=Homo sapiens, GI310109920, Length=289, Percent_Identity=29.4117647058824, Blast_Score=138, Evalue=6e-33, Organism=Homo sapiens, GI45446745, Length=289, Percent_Identity=29.4117647058824, Blast_Score=137, Evalue=7e-33, Organism=Homo sapiens, GI4503285, Length=289, Percent_Identity=29.4117647058824, Blast_Score=137, Evalue=7e-33, Organism=Homo sapiens, GI24497585, Length=289, Percent_Identity=29.0657439446367, Blast_Score=137, Evalue=1e-32, Organism=Homo sapiens, GI5453543, Length=289, Percent_Identity=29.0657439446367, Blast_Score=137, Evalue=1e-32, Organism=Homo sapiens, GI5174695, Length=292, Percent_Identity=30.1369863013699, Blast_Score=132, Evalue=4e-31, Organism=Homo sapiens, GI24497583, Length=294, Percent_Identity=30.2721088435374, Blast_Score=130, Evalue=8e-31, Organism=Homo sapiens, GI4502049, Length=289, Percent_Identity=32.5259515570934, Blast_Score=130, Evalue=2e-30, Organism=Homo sapiens, GI300116273, Length=268, Percent_Identity=30.9701492537313, Blast_Score=123, Evalue=1e-28, Organism=Homo sapiens, GI300116271, Length=277, Percent_Identity=29.6028880866426, Blast_Score=115, Evalue=3e-26, Organism=Homo sapiens, GI310109924, Length=152, Percent_Identity=28.2894736842105, Blast_Score=79, Evalue=5e-15, Organism=Homo sapiens, GI310109926, Length=119, Percent_Identity=36.1344537815126, Blast_Score=74, Evalue=1e-13, Organism=Homo sapiens, GI207028673, Length=119, Percent_Identity=36.1344537815126, Blast_Score=74, Evalue=1e-13, Organism=Homo sapiens, GI27436969, Length=309, Percent_Identity=23.9482200647249, Blast_Score=68, Evalue=7e-12, Organism=Homo sapiens, GI4504825, Length=309, Percent_Identity=23.9482200647249, Blast_Score=68, Evalue=8e-12, Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=70.4119850187266, Blast_Score=385, Evalue=1e-108, Organism=Escherichia coli, GI87082198, Length=256, Percent_Identity=38.28125, Blast_Score=176, Evalue=1e-45, Organism=Escherichia coli, GI1788081, Length=279, Percent_Identity=31.8996415770609, Blast_Score=114, Evalue=5e-27, Organism=Escherichia coli, GI48994888, Length=242, Percent_Identity=29.3388429752066, Blast_Score=72, Evalue=3e-14, Organism=Escherichia coli, GI1788070, Length=310, Percent_Identity=22.9032258064516, Blast_Score=69, Evalue=5e-13, Organism=Escherichia coli, GI1789199, Length=337, Percent_Identity=25.2225519287834, Blast_Score=67, Evalue=1e-12, Organism=Escherichia coli, GI1787674, Length=287, Percent_Identity=26.8292682926829, Blast_Score=62, Evalue=3e-11, Organism=Caenorhabditis elegans, GI17550248, Length=286, Percent_Identity=32.8671328671329, Blast_Score=150, Evalue=6e-37, Organism=Caenorhabditis elegans, GI17561298, Length=275, Percent_Identity=29.4545454545455, Blast_Score=139, Evalue=1e-33, Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=32.4137931034483, Blast_Score=138, Evalue=3e-33, Organism=Caenorhabditis elegans, GI71998625, Length=263, Percent_Identity=30.7984790874525, Blast_Score=137, Evalue=8e-33, Organism=Caenorhabditis elegans, GI17537077, Length=287, Percent_Identity=31.3588850174216, Blast_Score=133, Evalue=1e-31, Organism=Caenorhabditis elegans, GI17566692, Length=282, Percent_Identity=31.5602836879433, Blast_Score=129, Evalue=2e-30, Organism=Caenorhabditis elegans, GI17552492, Length=273, Percent_Identity=30.03663003663, Blast_Score=128, Evalue=3e-30, Organism=Caenorhabditis elegans, GI17561300, Length=274, Percent_Identity=29.1970802919708, Blast_Score=124, Evalue=5e-29, Organism=Caenorhabditis elegans, GI17562292, Length=270, Percent_Identity=30.3703703703704, Blast_Score=123, Evalue=1e-28, Organism=Caenorhabditis elegans, GI17538386, Length=265, Percent_Identity=30.188679245283, Blast_Score=120, Evalue=7e-28, Organism=Caenorhabditis elegans, GI17564128, Length=293, Percent_Identity=27.3037542662116, Blast_Score=119, Evalue=1e-27, Organism=Caenorhabditis elegans, GI17537079, Length=300, Percent_Identity=27, Blast_Score=117, Evalue=7e-27, Organism=Saccharomyces cerevisiae, GI6324694, Length=292, Percent_Identity=31.8493150684932, Blast_Score=161, Evalue=9e-41, Organism=Saccharomyces cerevisiae, GI6320576, Length=282, Percent_Identity=34.0425531914894, Blast_Score=148, Evalue=1e-36, Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=31.0457516339869, Blast_Score=135, Evalue=7e-33, Organism=Saccharomyces cerevisiae, GI6322556, Length=259, Percent_Identity=27.027027027027, Blast_Score=126, Evalue=3e-30, Organism=Saccharomyces cerevisiae, GI6319625, Length=275, Percent_Identity=29.8181818181818, Blast_Score=108, Evalue=1e-24, Organism=Saccharomyces cerevisiae, GI6320079, Length=244, Percent_Identity=29.0983606557377, Blast_Score=87, Evalue=3e-18, Organism=Drosophila melanogaster, GI24662789, Length=297, Percent_Identity=31.6498316498317, Blast_Score=137, Evalue=6e-33, Organism=Drosophila melanogaster, GI24644950, Length=298, Percent_Identity=32.5503355704698, Blast_Score=135, Evalue=2e-32, Organism=Drosophila melanogaster, GI24657054, Length=289, Percent_Identity=29.757785467128, Blast_Score=130, Evalue=1e-30, Organism=Drosophila melanogaster, GI221378297, Length=317, Percent_Identity=30.5993690851735, Blast_Score=129, Evalue=2e-30, Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=30.4195804195804, Blast_Score=123, Evalue=1e-28, Organism=Drosophila melanogaster, GI21356425, Length=284, Percent_Identity=28.169014084507, Blast_Score=122, Evalue=3e-28, Organism=Drosophila melanogaster, GI24662785, Length=288, Percent_Identity=31.9444444444444, Blast_Score=121, Evalue=6e-28, Organism=Drosophila melanogaster, GI45553081, Length=284, Percent_Identity=27.8169014084507, Blast_Score=118, Evalue=4e-27, Organism=Drosophila melanogaster, GI24662781, Length=288, Percent_Identity=31.9444444444444, Blast_Score=118, Evalue=5e-27, Organism=Drosophila melanogaster, GI24663317, Length=284, Percent_Identity=26.7605633802817, Blast_Score=111, Evalue=6e-25, Organism=Drosophila melanogaster, GI281366140, Length=258, Percent_Identity=26.7441860465116, Blast_Score=100, Evalue=2e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 29142; Mature: 29011
Theoretical pI: Translated: 4.92; Mature: 4.92
Prosite motif: PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 2.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSVPAFGLGTFRLQGQVVIDSVSTALELGYRVIDTAQIYENEADVGEAIAASGIARDKLF CCCCCCCCEEEEEECEEEEHHHHHHHHHCHHHEEHHHHHCCCCHHHHHHHHCCCCCCEEE ITSKIWIANFAKDRLIESLKASLQKLQTDYLDLTLIHWPSPEDQVPVEEFMGALLEAKRL EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC GLTRQIGVSNFTIDLMKQAIAAVGAENIATNQIELHPYLQNRKVVEFAQSQGIQITSYMT CCEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHCCEEEEHHHH LAYGEVLKDPLIVQIAERLQATPAQVTLAWAMQSGYAVIPSSTKRANLQGNLGAMALTLS HHHHHHHHCCHHHHHHHHHHCCCHHEEEEEEECCCCEEECCCCCCCCCCCCCCEEEEEEC DADMALIATLDRGHRLTSPKGIAPQWD CCCEEEEEEECCCCEECCCCCCCCCCC >Mature Secondary Structure SVPAFGLGTFRLQGQVVIDSVSTALELGYRVIDTAQIYENEADVGEAIAASGIARDKLF CCCCCCCEEEEEECEEEEHHHHHHHHHCHHHEEHHHHHCCCCHHHHHHHHCCCCCCEEE ITSKIWIANFAKDRLIESLKASLQKLQTDYLDLTLIHWPSPEDQVPVEEFMGALLEAKRL EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC GLTRQIGVSNFTIDLMKQAIAAVGAENIATNQIELHPYLQNRKVVEFAQSQGIQITSYMT CCEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHCCEEEEHHHH LAYGEVLKDPLIVQIAERLQATPAQVTLAWAMQSGYAVIPSSTKRANLQGNLGAMALTLS HHHHHHHHCCHHHHHHHHHHCCCHHEEEEEEECCCCEEECCCCCCCCCCCCCCEEEEEEC DADMALIATLDRGHRLTSPKGIAPQWD CCCEEEEEEECCCCEECCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11586360; 12142430 [H]