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The map label for this gene is ligA

Identifier: 77458028

GI number: 77458028

Start: 2010582

End: 2012939

Strand: Direct

Name: ligA

Synonym: Pfl01_1801

Alternate gene names: 77458028

Gene position: 2010582-2012939 (Clockwise)

Preceding gene: 77458027

Following gene: 77458029

Centisome position: 31.23

GC content: 62.72

Gene sequence:

>2358_bases
ATGACTGCCGCCAAAGACCGCATTCTAGAGCTGCGCGCTGAACTCGATCAGCACAACTACCGTTACCACGTCCTCGATGA
GCCGAGCATTCCGGATGCCGAGTACGACCGGTTGTTCCACGAGCTCAAGGCGCTGGAAGCGGCCAACCCGGAACTGATCA
CCAGCGACTCGCCGACCCAGCGCGTCGGCAGCGTGGCGCTGACCGCGTTCACCCAGGTGCGTCACGAAGTGCCGATGCTC
AGCCTCGGCAACGCCTTCGAAGAAACCGACATGCGCGAGTTCGACCGCCGGGTGACCGAAGGTCTGGATCTGCCGGCCGG
CGATCTGTTCGGTGGTGGCGCGGCGGTGGAATACAGCTGCGAGCCGAAACTCGATGGTCTGGCGGTCAGCCTGCTGTATC
AGGACGGCGTGCTGGTGCGCGGTGCCACGCGCGGCGACGGCACCACCGGCGAAGACATCAGCGTCAACGTGCGCACCGTG
CGCAACATTCCGCTGAAGCTGCACGGCACGGGCTGGCCGGCGACCCTGGAAGTGCGTGGCGAAGTGTTCATGTCCAAGGC
CGGTTTCGAACGCCTCAACGCTTCGCAACTGGAAGTCGGCGGCAAGACTTTCGCCAACCCGCGCAACGCCGCCGCCGGCA
GCCTGCGTCAGCTGGATTCGAAGATCACCGCCAACCGGCCGCTGGAATTCTGCTGCTACGGCATCGGCCAGGTGTCCCAC
GATATTTCCGACACCCACATCGGCAACCTCCAGCAACTGAAAGCCTGGGGCATGCCGATCAGCCACGAATTGAAACTGGC
CAAGGGCATCGGCGAATGCCTGGATTATTACCGCGATATCGGTGAGCGCCGTAATTCGCTGGCCTATGAAATCGACGGCG
TGGTGTTCAAGGTAAACAGCATTGCCGACCAGCGCGAACTGGGCTTCCGCGCCCGTGAACCGCGCTGGGCCATCGCCCAC
AAATTCCCGGCCATGGAAGAGCTCACCGAGCTGCTCGACGTGGAGTTCCAGGTCGGCCGTACCGGCGCCGTCACCCCTGT
GGCGCGCTTGAAACCGGTCAAGGTCGCTGGCGTCACGGTGGCCAACGCCACATTGCACAACATGGATGAAGTCGCCCGGC
TGGGCCTGATGATCGGCGACACGGTGATCATTCGTCGCGCTGGCGATGTGATCCCGCAAGTGGTGCAAGTGGTCATGGAG
CGCCGCCCGGAAGATGCGCGGCCGGTGCAGATTCCTGAGAGCTGCCCGGTCTGCGGCTCCCACGTCGAACGCACGCAACT
GGTCAAGCGCAGCAAGGGCAAGGAAACCATCAGCGAGGGCGCGGTGTATCGCTGCGTCGGCCGACTGGCCTGCGGTGCGC
AACTGAAGCAGGCGATCATTCACTTCGTCTCCCGTCGTGCGATGGACATCGAAGGCCTGGGCGACAAGAGTGTCGAGCAG
TTGGTCGATGAGGGGCTGGTCAGTTCGCCGGCTGATCTTTATGCGCTGAAGTTCGAGGACATCGTCGACCTCGAGGGCTT
TGCCGAGGTGTCGAGCAACAAGTTGCTGGCTGCGATTGAAGACAGCAAGAAACCGGGTCTGGCGCGCTTCATCTATGCGC
TGGGCATTCCAGATGTCGGCGAAGAGACGGCCAAGGTGCTGGCGCGTTCGCTGGGGTCGCTGGAGCGTGTTCAGCAAGCC
TTGCCGCAAGTGCTGACTTACCTGCCGGACATCGGTCTGGAAGTGGCGCACGAGATTCACAGCTTCTTCGAAGATGCGCA
CAACCGGCAGGTGATCACCGAGCTGCTGGGCCACGGTTTGCAGATTCAGGATCAGGGCGAGCTGGGCGCCGAGTTTGCCG
CCAGCACCACGCTGGGTGGCTTCCTCGACAAGCTGCACATTCCCTCGGTGGGCCCGGGCGGGGCGCAGAAGCTGGCGGAC
AAGTTCGAATCGCTGGAAGGGGTGATGAATGCGGACTGGCTGGACATGCGCCAGGCATTGCCGGAGAAGCAGGCCAACTC
GGTTCGCGAGTTCTTCGCCTTGCCAGAACATCGTCAACTGGCTGAAGACGCCGAGAAGCAACTGCGCGATTTCGGTATGC
ACTGGCAGAGCGAAAAGAAAGTCGTCGAAGGCCTGCCATTGGCCGGGGAGACCTGGGTACTGACCGGCAAGGTCGAGCTG
ATGAGCCGCGACGTGGCCAAGGAACACCTGGAAAGCCTCGGCGCCAAGGTCGCCGGCTCCGTGTCGGCCAAGACTCATTG
CGTGGTCGCCGGGCCGGGCGCCGGGTCGAAACTGACCAAGGCCAACGAACTGGGCGTGAAAGTGATGGATGAAGAGGCGT
TTATCGCGTTCCTGAAAGGACACGGTATTTCCGCCTGA

Upstream 100 bases:

>100_bases
CCAGAAGCGTTGATTGAAAAGATTGCCTCGCAATAAAGGGGCAATCGGCAAAATAGATTGAGCAGCCTCGGCTGCTCTTT
TGCTTTATGAGAGAACACCC

Downstream 100 bases:

>100_bases
CGCAACGCGCGGGAACGATCGACTCGCGGGAATGATCTAGTCTTGGCAAGCCCAAGGGAGAGATCGCCATGTACCGCTTT
TTCGAGCAGCTCAGTTCCCG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 785; Mature: 784

Protein sequence:

>785_residues
MTAAKDRILELRAELDQHNYRYHVLDEPSIPDAEYDRLFHELKALEAANPELITSDSPTQRVGSVALTAFTQVRHEVPML
SLGNAFEETDMREFDRRVTEGLDLPAGDLFGGGAAVEYSCEPKLDGLAVSLLYQDGVLVRGATRGDGTTGEDISVNVRTV
RNIPLKLHGTGWPATLEVRGEVFMSKAGFERLNASQLEVGGKTFANPRNAAAGSLRQLDSKITANRPLEFCCYGIGQVSH
DISDTHIGNLQQLKAWGMPISHELKLAKGIGECLDYYRDIGERRNSLAYEIDGVVFKVNSIADQRELGFRAREPRWAIAH
KFPAMEELTELLDVEFQVGRTGAVTPVARLKPVKVAGVTVANATLHNMDEVARLGLMIGDTVIIRRAGDVIPQVVQVVME
RRPEDARPVQIPESCPVCGSHVERTQLVKRSKGKETISEGAVYRCVGRLACGAQLKQAIIHFVSRRAMDIEGLGDKSVEQ
LVDEGLVSSPADLYALKFEDIVDLEGFAEVSSNKLLAAIEDSKKPGLARFIYALGIPDVGEETAKVLARSLGSLERVQQA
LPQVLTYLPDIGLEVAHEIHSFFEDAHNRQVITELLGHGLQIQDQGELGAEFAASTTLGGFLDKLHIPSVGPGGAQKLAD
KFESLEGVMNADWLDMRQALPEKQANSVREFFALPEHRQLAEDAEKQLRDFGMHWQSEKKVVEGLPLAGETWVLTGKVEL
MSRDVAKEHLESLGAKVAGSVSAKTHCVVAGPGAGSKLTKANELGVKVMDEEAFIAFLKGHGISA

Sequences:

>Translated_785_residues
MTAAKDRILELRAELDQHNYRYHVLDEPSIPDAEYDRLFHELKALEAANPELITSDSPTQRVGSVALTAFTQVRHEVPML
SLGNAFEETDMREFDRRVTEGLDLPAGDLFGGGAAVEYSCEPKLDGLAVSLLYQDGVLVRGATRGDGTTGEDISVNVRTV
RNIPLKLHGTGWPATLEVRGEVFMSKAGFERLNASQLEVGGKTFANPRNAAAGSLRQLDSKITANRPLEFCCYGIGQVSH
DISDTHIGNLQQLKAWGMPISHELKLAKGIGECLDYYRDIGERRNSLAYEIDGVVFKVNSIADQRELGFRAREPRWAIAH
KFPAMEELTELLDVEFQVGRTGAVTPVARLKPVKVAGVTVANATLHNMDEVARLGLMIGDTVIIRRAGDVIPQVVQVVME
RRPEDARPVQIPESCPVCGSHVERTQLVKRSKGKETISEGAVYRCVGRLACGAQLKQAIIHFVSRRAMDIEGLGDKSVEQ
LVDEGLVSSPADLYALKFEDIVDLEGFAEVSSNKLLAAIEDSKKPGLARFIYALGIPDVGEETAKVLARSLGSLERVQQA
LPQVLTYLPDIGLEVAHEIHSFFEDAHNRQVITELLGHGLQIQDQGELGAEFAASTTLGGFLDKLHIPSVGPGGAQKLAD
KFESLEGVMNADWLDMRQALPEKQANSVREFFALPEHRQLAEDAEKQLRDFGMHWQSEKKVVEGLPLAGETWVLTGKVEL
MSRDVAKEHLESLGAKVAGSVSAKTHCVVAGPGAGSKLTKANELGVKVMDEEAFIAFLKGHGISA
>Mature_784_residues
TAAKDRILELRAELDQHNYRYHVLDEPSIPDAEYDRLFHELKALEAANPELITSDSPTQRVGSVALTAFTQVRHEVPMLS
LGNAFEETDMREFDRRVTEGLDLPAGDLFGGGAAVEYSCEPKLDGLAVSLLYQDGVLVRGATRGDGTTGEDISVNVRTVR
NIPLKLHGTGWPATLEVRGEVFMSKAGFERLNASQLEVGGKTFANPRNAAAGSLRQLDSKITANRPLEFCCYGIGQVSHD
ISDTHIGNLQQLKAWGMPISHELKLAKGIGECLDYYRDIGERRNSLAYEIDGVVFKVNSIADQRELGFRAREPRWAIAHK
FPAMEELTELLDVEFQVGRTGAVTPVARLKPVKVAGVTVANATLHNMDEVARLGLMIGDTVIIRRAGDVIPQVVQVVMER
RPEDARPVQIPESCPVCGSHVERTQLVKRSKGKETISEGAVYRCVGRLACGAQLKQAIIHFVSRRAMDIEGLGDKSVEQL
VDEGLVSSPADLYALKFEDIVDLEGFAEVSSNKLLAAIEDSKKPGLARFIYALGIPDVGEETAKVLARSLGSLERVQQAL
PQVLTYLPDIGLEVAHEIHSFFEDAHNRQVITELLGHGLQIQDQGELGAEFAASTTLGGFLDKLHIPSVGPGGAQKLADK
FESLEGVMNADWLDMRQALPEKQANSVREFFALPEHRQLAEDAEKQLRDFGMHWQSEKKVVEGLPLAGETWVLTGKVELM
SRDVAKEHLESLGAKVAGSVSAKTHCVVAGPGAGSKLTKANELGVKVMDEEAFIAFLKGHGISA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=633, Percent_Identity=54.1864139020537, Blast_Score=671, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_PSEPF (Q3KFB2)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_347533.1
- ProteinModelPortal:   Q3KFB2
- STRING:   Q3KFB2
- GeneID:   3716561
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_1801
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   FHELKAL
- ProtClustDB:   PRK07956
- BioCyc:   PFLU205922:PFL_1801-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 85673; Mature: 85541

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 123-123 BINDING 121-121 BINDING 144-144 BINDING 181-181 BINDING 297-297 BINDING 321-321

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAKDRILELRAELDQHNYRYHVLDEPSIPDAEYDRLFHELKALEAANPELITSDSPTQ
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCHH
RVGSVALTAFTQVRHEVPMLSLGNAFEETDMREFDRRVTEGLDLPAGDLFGGGAAVEYSC
HHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
EPKLDGLAVSLLYQDGVLVRGATRGDGTTGEDISVNVRTVRNIPLKLHGTGWPATLEVRG
CCCCCCEEEEEEECCCEEEEECCCCCCCCCCCEEEEEEEEECCCEEEECCCCCEEEEECC
EVFMSKAGFERLNASQLEVGGKTFANPRNAAAGSLRQLDSKITANRPLEFCCYGIGQVSH
HHHHHHHCHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
DISDTHIGNLQQLKAWGMPISHELKLAKGIGECLDYYRDIGERRNSLAYEIDGVVFKVNS
CCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECC
IADQRELGFRAREPRWAIAHKFPAMEELTELLDVEFQVGRTGAVTPVARLKPVKVAGVTV
CCCHHHCCCCCCCCCEEHHHCCCCHHHHHHHHCCHHCCCCCCCCCHHHHCCCEEEECEEE
ANATLHNMDEVARLGLMIGDTVIIRRAGDVIPQVVQVVMERRPEDARPVQIPESCPVCGS
ECHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
HVERTQLVKRSKGKETISEGAVYRCVGRLACGAQLKQAIIHFVSRRAMDIEGLGDKSVEQ
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LVDEGLVSSPADLYALKFEDIVDLEGFAEVSSNKLLAAIEDSKKPGLARFIYALGIPDVG
HHHCCCCCCCCCEEEEEHHHHHCCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCC
EETAKVLARSLGSLERVQQALPQVLTYLPDIGLEVAHEIHSFFEDAHNRQVITELLGHGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCC
QIQDQGELGAEFAASTTLGGFLDKLHIPSVGPGGAQKLADKFESLEGVMNADWLDMRQAL
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHC
PEKQANSVREFFALPEHRQLAEDAEKQLRDFGMHWQSEKKVVEGLPLAGETWVLTGKVEL
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEECHHHH
MSRDVAKEHLESLGAKVAGSVSAKTHCVVAGPGAGSKLTKANELGVKVMDEEAFIAFLKG
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHCCCEEECCHHEEEEEEC
HGISA
CCCCC
>Mature Secondary Structure 
TAAKDRILELRAELDQHNYRYHVLDEPSIPDAEYDRLFHELKALEAANPELITSDSPTQ
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCHH
RVGSVALTAFTQVRHEVPMLSLGNAFEETDMREFDRRVTEGLDLPAGDLFGGGAAVEYSC
HHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
EPKLDGLAVSLLYQDGVLVRGATRGDGTTGEDISVNVRTVRNIPLKLHGTGWPATLEVRG
CCCCCCEEEEEEECCCEEEEECCCCCCCCCCCEEEEEEEEECCCEEEECCCCCEEEEECC
EVFMSKAGFERLNASQLEVGGKTFANPRNAAAGSLRQLDSKITANRPLEFCCYGIGQVSH
HHHHHHHCHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
DISDTHIGNLQQLKAWGMPISHELKLAKGIGECLDYYRDIGERRNSLAYEIDGVVFKVNS
CCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECC
IADQRELGFRAREPRWAIAHKFPAMEELTELLDVEFQVGRTGAVTPVARLKPVKVAGVTV
CCCHHHCCCCCCCCCEEHHHCCCCHHHHHHHHCCHHCCCCCCCCCHHHHCCCEEEECEEE
ANATLHNMDEVARLGLMIGDTVIIRRAGDVIPQVVQVVMERRPEDARPVQIPESCPVCGS
ECHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
HVERTQLVKRSKGKETISEGAVYRCVGRLACGAQLKQAIIHFVSRRAMDIEGLGDKSVEQ
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LVDEGLVSSPADLYALKFEDIVDLEGFAEVSSNKLLAAIEDSKKPGLARFIYALGIPDVG
HHHCCCCCCCCCEEEEEHHHHHCCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCC
EETAKVLARSLGSLERVQQALPQVLTYLPDIGLEVAHEIHSFFEDAHNRQVITELLGHGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCC
QIQDQGELGAEFAASTTLGGFLDKLHIPSVGPGGAQKLADKFESLEGVMNADWLDMRQAL
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHC
PEKQANSVREFFALPEHRQLAEDAEKQLRDFGMHWQSEKKVVEGLPLAGETWVLTGKVEL
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEECHHHH
MSRDVAKEHLESLGAKVAGSVSAKTHCVVAGPGAGSKLTKANELGVKVMDEEAFIAFLKG
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHCCCEEECCHHEEEEEEC
HGISA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA