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The map label for this gene is nasA [H]

Identifier: 77458010

GI number: 77458010

Start: 1984161

End: 1985372

Strand: Reverse

Name: nasA [H]

Synonym: Pfl01_1783

Alternate gene names: 77458010

Gene position: 1985372-1984161 (Counterclockwise)

Preceding gene: 77458011

Following gene: 77458009

Centisome position: 30.84

GC content: 63.7

Gene sequence:

>1212_bases
ATGAATAAAAGCTTCTGGAAATCCGGCCACACCCCGACCCTGTTCGCGGCCTTCCTGTATTTCGACCTGAGCTTCATGGT
CTGGTACCTGCTCGGCCCGCTGGCGGTGCAGATTGCTGGCGATCTGCACCTGACTACCCAGCAGCGCGGTCTTGTGGTCG
CCACGCCGATTCTGGCCGGCGCGGTACTGCGTTTTGCGATGGGCATGCTCGCCGATCGCCTGTCGCCGAAAACCGCCGGG
CTGATCGGCCAGGTGATCGTGATCTGTGCGCTGTTCGGTGCCTGGAAACTGGGCATCCACAGTTATGAACAGGCCCTGCT
GCTCGGTTTGTTCCTCGGTATGGCCGGCGCCTCGTTCGCCGTGGCCCTGCCCCTGGCCTCGCAGTGGTATCCGCCGGAGC
ATCAGGGCAAGGCGATGGGCATCGCCGGTGCCGGCAACTCCGGCACCGTATTCGCGGCACTGATCGCCCCGGTGCTGGCG
GCGGCGTTTGGCTGGAGCAATGTATTCGGTTTCGCCCTGATCCCGTTGATCCTGACCCTGGTGCTGTTCGCCTGGCTGGC
GAAAAACGCGCCGGAGCGACCAAGAGCCAAAGCCATGGCCGACTACTTCAAGGCTTTGGGTGATCGCGACAGCTGGTGGT
TCATGTTTTTCTACAGCGTCACCTTCGGCGGCTTCATCGGCCTGGCCAGCGCCCTGCCCGGCTACTTCAACGATCAATAC
GGCCTGAGCCCGGTGACCGCCGGTTACTACACCGCCGCCTGCGTCTTCGGCGGCAGCCTGATGCGTCCCCTCGGTGGCGC
ACTGGCTGACCGATTCGGTGGTATTCGCACGCTTTTGGCGATGTACACGGTCGCCGCAGTTTGTATCGCGGCGGTCGGTT
TCAACCTGCCAAGTTCCTACGCGGCGCTGGCACTTTTCGTCTGCACCATGCTCGGTCTCGGCGCGGGTAACGGCGCGGTG
TTCCAACTGGTGCCGCAACGTTTCCGTCGCGAAATCGGCGTGATGACCGGGCTGATCGGCATGGCCGGCGGTATCGGCGG
TTTTGCCCTGGCGGCAGGCATGGGCGCGATCAAACAGAGCACCGGCAGCTATCAACTGGCGCTGTGGTTGTTCGCCAGCC
TTGGCGTGTTGGCCTGGTTCGGTCTGCACGGGGTCAAACGTCGCTGGAGAACCACTTGGGGTTCGGCGGCCGTGACCGCT
GCCCGGGTCTGA

Upstream 100 bases:

>100_bases
GTTTTGGCCCCACAGCCCGGGGCCGGTGGTGCGGCCGTGGCGGCGCCCCACTGTGCTGGTTCTGACTCCTTCTCGAGATT
CTCTACAGCTGAGGTGCGCG

Downstream 100 bases:

>100_bases
GGCCCGAATGGCTCTGCAACTGAGCTTCGCCGAAGCCAGCGCCACAGGCCCCCGCGCGGAAAATCAGGACGCCTTGCGCC
TGGTCACGCCGGCCCCGGCG

Product: nitrate transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MNKSFWKSGHTPTLFAAFLYFDLSFMVWYLLGPLAVQIAGDLHLTTQQRGLVVATPILAGAVLRFAMGMLADRLSPKTAG
LIGQVIVICALFGAWKLGIHSYEQALLLGLFLGMAGASFAVALPLASQWYPPEHQGKAMGIAGAGNSGTVFAALIAPVLA
AAFGWSNVFGFALIPLILTLVLFAWLAKNAPERPRAKAMADYFKALGDRDSWWFMFFYSVTFGGFIGLASALPGYFNDQY
GLSPVTAGYYTAACVFGGSLMRPLGGALADRFGGIRTLLAMYTVAAVCIAAVGFNLPSSYAALALFVCTMLGLGAGNGAV
FQLVPQRFRREIGVMTGLIGMAGGIGGFALAAGMGAIKQSTGSYQLALWLFASLGVLAWFGLHGVKRRWRTTWGSAAVTA
ARV

Sequences:

>Translated_403_residues
MNKSFWKSGHTPTLFAAFLYFDLSFMVWYLLGPLAVQIAGDLHLTTQQRGLVVATPILAGAVLRFAMGMLADRLSPKTAG
LIGQVIVICALFGAWKLGIHSYEQALLLGLFLGMAGASFAVALPLASQWYPPEHQGKAMGIAGAGNSGTVFAALIAPVLA
AAFGWSNVFGFALIPLILTLVLFAWLAKNAPERPRAKAMADYFKALGDRDSWWFMFFYSVTFGGFIGLASALPGYFNDQY
GLSPVTAGYYTAACVFGGSLMRPLGGALADRFGGIRTLLAMYTVAAVCIAAVGFNLPSSYAALALFVCTMLGLGAGNGAV
FQLVPQRFRREIGVMTGLIGMAGGIGGFALAAGMGAIKQSTGSYQLALWLFASLGVLAWFGLHGVKRRWRTTWGSAAVTA
ARV
>Mature_403_residues
MNKSFWKSGHTPTLFAAFLYFDLSFMVWYLLGPLAVQIAGDLHLTTQQRGLVVATPILAGAVLRFAMGMLADRLSPKTAG
LIGQVIVICALFGAWKLGIHSYEQALLLGLFLGMAGASFAVALPLASQWYPPEHQGKAMGIAGAGNSGTVFAALIAPVLA
AAFGWSNVFGFALIPLILTLVLFAWLAKNAPERPRAKAMADYFKALGDRDSWWFMFFYSVTFGGFIGLASALPGYFNDQY
GLSPVTAGYYTAACVFGGSLMRPLGGALADRFGGIRTLLAMYTVAAVCIAAVGFNLPSSYAALALFVCTMLGLGAGNGAV
FQLVPQRFRREIGVMTGLIGMAGGIGGFALAAGMGAIKQSTGSYQLALWLFASLGVLAWFGLHGVKRRWRTTWGSAAVTA
ARV

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=420, Percent_Identity=26.9047619047619, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787743, Length=419, Percent_Identity=25.7756563245823, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42623; Mature: 42623

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]