Click here to switch to the map view.

The map label for this gene is nasD [H]

Identifier: 77458008

GI number: 77458008

Start: 1979720

End: 1982191

Strand: Reverse

Name: nasD [H]

Synonym: Pfl01_1781

Alternate gene names: 77458008

Gene position: 1982191-1979720 (Counterclockwise)

Preceding gene: 77458009

Following gene: 77458007

Centisome position: 30.79

GC content: 60.76

Gene sequence:

>2472_bases
ATGCTCCCGGAGAACCTGATGAAAAAACTCAAACTGGTGATGATCGGCAATGGCATGGCCGGGGTTCGCACCCTGGAAGA
ACTGCTGAAACTGAGCAACGAGCTGTACGACATCACGGTCTTCGGCGCCGAACCGCACACCAACTACAACCGCATCCTGT
TGTCGCCGGTACTGGCCGGCGAACAGACCTTCGAAGAGATCGTGCTCAACGACCTCGACTGGTATCTGGAAAACAACATC
AAACTGCTGCTCAACCGCAAAGTGGTGGAGATCGACCGGGTCAAACGCCGAGTGATCGCCGAGGATGGCACCGAAGCCGA
ATACGATCGTCTGCTGATCGCCACAGGTTCGACCCCGTTCATCCTGCCGATCCCCGGCAACACCTTGCAGGGCGTGATCG
GCTACCGCGACATCGCCGACACCCAGGCGATGATCGACACTGCCAAGACCCACAAGCACGCCGTGGTGATCGGCGGCGGC
CTGCTCGGTCTCGAAGCGGCCAACGGCCTGATGCTGCGCGGCATGCACGTCACCGTGGTGCACCTCGGTGAATGGCTGCT
GGAGCGGCAACTGGACAAGACCAGCGGCCAACTTTTGCAAACCGCCCTAGAATCCCGTGGCCTGCACTTTCGCCTGTGTG
AACAGACCCAGGCCCTGCACGACGCCGGCAATGGTCGGGTCGGCTCGGTGCAGTTCAAGAACGGCGACATCATTCCTGCC
GATCTGGTGGTCATGGCCGCCGGCATCCGCCCCAATACCGAACTGGCGGAAAAGGCCGGCATCCCGTGCAACCGCGGGAT
TCTGGTCAACGACACCCTGCAAACCTACGACCCACGCATCTATGCGATCGGCGAATGCGCCAGCCACCGTGGCATCGCCT
ACGGCCTGGTGGCGCCGCTGTTTGAACAGGCCAAGGTCTGCGCCAACCACCTGGCGCAACTGGGTTTCGCCCGTTATCAG
GGCTCGGTGACGTCGACCAAACTGAAGGTTACCGGCATCGATCTGTTTTCCGCTGGCGACTTCATGGGCGGCGAAGGCAC
CGAGACCATTACCCTCTCCGACCCGATCGGTGGGGTGTACAAAAAACTGGTGATCAAGGATGACGTGCTGGTCGGCGCCT
GTCTGTACGGCGATACGGCAGATGGCGGTTGGTATTTCCGGCAGATTCGTGAGAATCACGCCATCGGCGAGATCCGCGAT
CACCTGATGTTTGGAGAAAATGCACTTGGCGATGTAGGACATCAGGGCCAGGACAAAGCCATGAGCATGGCCGACAACGC
CGAAGTCTGCGGCTGCAACGGCGTGTGCAAGGGCACCATCGTCAAGGCGATTCAGGAACACGGCCTGTTCAGCGTCGACG
AGGTGAAGAAACACACCAAGGCCGCCAGCTCCTGCGGCTCCTGCGCCGGGCTGGTCGAGCAGATCCTGATCAACACCGTC
GGCGGTGCGGCGGACGTCAAACCTAAAAGCGAAAAAGCCATCTGCGGTTGCAGCGACCTCAACCACGGCCAGATTCGCCA
GGCGATTCGCGAACAGCATCTGCTGACCATCGCCGGCACCATGAGCTACCTCAACTGGCGCACCCCGAACGGCTGCGCCA
CCTGCCGCCCGGCCCTCAACTACTACCTGATTTCCACCTGGCCCGGCGAGGCCAAGGACGATCCGCAGTCGCGCCTGATC
AACGAGCGGGCCCACGCCAACATCCAGAAGGACGGCACTTATTCGGTGGTGCCGCGGATGTGGGGCGGCGTGACCAACCC
GTCGGAACTGCGTCGGATCGCCGACGTGGCGGACAAATACCAGGTGCCAATGGTCAAGGTCACCGGCGGCCAGCGCATCG
ACTTGCTGGGGATCAAGAAACAGGATTTGCCCGGCGTATGGAAAGACCTCGACATGCCGTCCGGCCACGCCTATGGCAAA
TCGATCCGCACGGTGAAAACCTGCGTCGGCAGCGAGTTCTGTCGCTTCGGCACGCAGAACTCGACCCAGTTGGGCATCGA
CCTCGAACACGACCTGTTCAACATGTGGTCGCCGCACAAAGTGAAACTGGCAGTCTCCGGATGCCCACGCAACTGCTCGG
AAGCAGGGATCAAGGACGTAGGCATCATCGGCGTCGACTCCGGCTGGGAGATGTATATCGGTGGCAATGGCGGGATCAAG
ACCGAAGTCGCCGAGTTCTTCGTCAAGCTCAAGACCGCCGAGGAAGTGCGCGAGTACAACGGCGCGTTCCTGCAGCTGTA
CCGCGAAGAAGCCTTCTACCTCGAACGCACCGTGCACTACCTGCAACGGGTCGGCATGGAGCACATCAAGAAAGCCGTGC
TGGAAGATCCCGAGCGTCGCAAGGCCCTGAACGAGCGCCTGCAATTCTCCCTGTCGTTCGAACAGGACCCGTGGAAAGAG
CGCCTTGCCCAGCCGCAACTGAAGAAAGAATTCGACGTGCTCCCTGTGAAAAACCTGGAGGTGCCAGCATGA

Upstream 100 bases:

>100_bases
CAAGGACAAAGGCGTCCTCGCTAGGTAACTGGCGGGACGCCTTTTTTGTTTGCGCCATTTTTGTCGAGCAAGGCCTGACT
GCCCACAAGAGGCAAGCCCG

Downstream 100 bases:

>100_bases
ACTGGCTCGATATCTGTGCCCTGGAAGAGATCAACGCCCTCGGTTCGCGGATCATCGCCGGGCCCAAAGGTGACATTGCG
ATCTTTCGCACAAGCGACGA

Product: assimilatory nitrite reductase (NAD(P)H) large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 823; Mature: 823

Protein sequence:

>823_residues
MLPENLMKKLKLVMIGNGMAGVRTLEELLKLSNELYDITVFGAEPHTNYNRILLSPVLAGEQTFEEIVLNDLDWYLENNI
KLLLNRKVVEIDRVKRRVIAEDGTEAEYDRLLIATGSTPFILPIPGNTLQGVIGYRDIADTQAMIDTAKTHKHAVVIGGG
LLGLEAANGLMLRGMHVTVVHLGEWLLERQLDKTSGQLLQTALESRGLHFRLCEQTQALHDAGNGRVGSVQFKNGDIIPA
DLVVMAAGIRPNTELAEKAGIPCNRGILVNDTLQTYDPRIYAIGECASHRGIAYGLVAPLFEQAKVCANHLAQLGFARYQ
GSVTSTKLKVTGIDLFSAGDFMGGEGTETITLSDPIGGVYKKLVIKDDVLVGACLYGDTADGGWYFRQIRENHAIGEIRD
HLMFGENALGDVGHQGQDKAMSMADNAEVCGCNGVCKGTIVKAIQEHGLFSVDEVKKHTKAASSCGSCAGLVEQILINTV
GGAADVKPKSEKAICGCSDLNHGQIRQAIREQHLLTIAGTMSYLNWRTPNGCATCRPALNYYLISTWPGEAKDDPQSRLI
NERAHANIQKDGTYSVVPRMWGGVTNPSELRRIADVADKYQVPMVKVTGGQRIDLLGIKKQDLPGVWKDLDMPSGHAYGK
SIRTVKTCVGSEFCRFGTQNSTQLGIDLEHDLFNMWSPHKVKLAVSGCPRNCSEAGIKDVGIIGVDSGWEMYIGGNGGIK
TEVAEFFVKLKTAEEVREYNGAFLQLYREEAFYLERTVHYLQRVGMEHIKKAVLEDPERRKALNERLQFSLSFEQDPWKE
RLAQPQLKKEFDVLPVKNLEVPA

Sequences:

>Translated_823_residues
MLPENLMKKLKLVMIGNGMAGVRTLEELLKLSNELYDITVFGAEPHTNYNRILLSPVLAGEQTFEEIVLNDLDWYLENNI
KLLLNRKVVEIDRVKRRVIAEDGTEAEYDRLLIATGSTPFILPIPGNTLQGVIGYRDIADTQAMIDTAKTHKHAVVIGGG
LLGLEAANGLMLRGMHVTVVHLGEWLLERQLDKTSGQLLQTALESRGLHFRLCEQTQALHDAGNGRVGSVQFKNGDIIPA
DLVVMAAGIRPNTELAEKAGIPCNRGILVNDTLQTYDPRIYAIGECASHRGIAYGLVAPLFEQAKVCANHLAQLGFARYQ
GSVTSTKLKVTGIDLFSAGDFMGGEGTETITLSDPIGGVYKKLVIKDDVLVGACLYGDTADGGWYFRQIRENHAIGEIRD
HLMFGENALGDVGHQGQDKAMSMADNAEVCGCNGVCKGTIVKAIQEHGLFSVDEVKKHTKAASSCGSCAGLVEQILINTV
GGAADVKPKSEKAICGCSDLNHGQIRQAIREQHLLTIAGTMSYLNWRTPNGCATCRPALNYYLISTWPGEAKDDPQSRLI
NERAHANIQKDGTYSVVPRMWGGVTNPSELRRIADVADKYQVPMVKVTGGQRIDLLGIKKQDLPGVWKDLDMPSGHAYGK
SIRTVKTCVGSEFCRFGTQNSTQLGIDLEHDLFNMWSPHKVKLAVSGCPRNCSEAGIKDVGIIGVDSGWEMYIGGNGGIK
TEVAEFFVKLKTAEEVREYNGAFLQLYREEAFYLERTVHYLQRVGMEHIKKAVLEDPERRKALNERLQFSLSFEQDPWKE
RLAQPQLKKEFDVLPVKNLEVPA
>Mature_823_residues
MLPENLMKKLKLVMIGNGMAGVRTLEELLKLSNELYDITVFGAEPHTNYNRILLSPVLAGEQTFEEIVLNDLDWYLENNI
KLLLNRKVVEIDRVKRRVIAEDGTEAEYDRLLIATGSTPFILPIPGNTLQGVIGYRDIADTQAMIDTAKTHKHAVVIGGG
LLGLEAANGLMLRGMHVTVVHLGEWLLERQLDKTSGQLLQTALESRGLHFRLCEQTQALHDAGNGRVGSVQFKNGDIIPA
DLVVMAAGIRPNTELAEKAGIPCNRGILVNDTLQTYDPRIYAIGECASHRGIAYGLVAPLFEQAKVCANHLAQLGFARYQ
GSVTSTKLKVTGIDLFSAGDFMGGEGTETITLSDPIGGVYKKLVIKDDVLVGACLYGDTADGGWYFRQIRENHAIGEIRD
HLMFGENALGDVGHQGQDKAMSMADNAEVCGCNGVCKGTIVKAIQEHGLFSVDEVKKHTKAASSCGSCAGLVEQILINTV
GGAADVKPKSEKAICGCSDLNHGQIRQAIREQHLLTIAGTMSYLNWRTPNGCATCRPALNYYLISTWPGEAKDDPQSRLI
NERAHANIQKDGTYSVVPRMWGGVTNPSELRRIADVADKYQVPMVKVTGGQRIDLLGIKKQDLPGVWKDLDMPSGHAYGK
SIRTVKTCVGSEFCRFGTQNSTQLGIDLEHDLFNMWSPHKVKLAVSGCPRNCSEAGIKDVGIIGVDSGWEMYIGGNGGIK
TEVAEFFVKLKTAEEVREYNGAFLQLYREEAFYLERTVHYLQRVGMEHIKKAVLEDPERRKALNERLQFSLSFEQDPWKE
RLAQPQLKKEFDVLPVKNLEVPA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=281, Percent_Identity=28.1138790035587, Blast_Score=106, Evalue=1e-22,
Organism=Homo sapiens, GI226437568, Length=281, Percent_Identity=28.1138790035587, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI21389617, Length=281, Percent_Identity=28.1138790035587, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1789765, Length=828, Percent_Identity=34.7826086956522, Blast_Score=421, Evalue=1e-118,
Organism=Escherichia coli, GI1789065, Length=301, Percent_Identity=28.9036544850498, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI1788892, Length=286, Percent_Identity=25.5244755244755, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=280, Percent_Identity=30.3571428571429, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI281359715, Length=323, Percent_Identity=24.4582043343653, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281359713, Length=323, Percent_Identity=24.4582043343653, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24639250, Length=323, Percent_Identity=24.4582043343653, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18543267, Length=323, Percent_Identity=24.4582043343653, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24639252, Length=323, Percent_Identity=24.4582043343653, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24639257, Length=323, Percent_Identity=24.4582043343653, Blast_Score=104, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585130, Length=330, Percent_Identity=23.030303030303, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21358499, Length=184, Percent_Identity=33.1521739130435, Blast_Score=74, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 90541; Mature: 90541

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPENLMKKLKLVMIGNGMAGVRTLEELLKLSNELYDITVFGAEPHTNYNRILLSPVLAG
CCCHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEHHHCC
EQTFEEIVLNDLDWYLENNIKLLLNRKVVEIDRVKRRVIAEDGTEAEYDRLLIATGSTPF
HHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCE
ILPIPGNTLQGVIGYRDIADTQAMIDTAKTHKHAVVIGGGLLGLEAANGLMLRGMHVTVV
EEECCCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCEEEHHCCCCEEEECCEEEEH
HLGEWLLERQLDKTSGQLLQTALESRGLHFRLCEQTQALHDAGNGRVGSVQFKNGDIIPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCEEEEEECCCCCCCH
DLVVMAAGIRPNTELAEKAGIPCNRGILVNDTLQTYDPRIYAIGECASHRGIAYGLVAPL
HHHHEECCCCCCHHHHHHCCCCCCCCEEEECCHHHCCCEEEEEECHHCCCCCHHHHHHHH
FEQAKVCANHLAQLGFARYQGSVTSTKLKVTGIDLFSAGDFMGGEGTETITLSDPIGGVY
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCCCCCEEEEECCCCCHHH
KKLVIKDDVLVGACLYGDTADGGWYFRQIRENHAIGEIRDHLMFGENALGDVGHQGQDKA
HHHHEECCEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHCCCCCCCCCHH
MSMADNAEVCGCNGVCKGTIVKAIQEHGLFSVDEVKKHTKAASSCGSCAGLVEQILINTV
HHHCCCCCEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGAADVKPKSEKAICGCSDLNHGQIRQAIREQHLLTIAGTMSYLNWRTPNGCATCRPALN
CCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCCCCCCCC
YYLISTWPGEAKDDPQSRLINERAHANIQKDGTYSVVPRMWGGVTNPSELRRIADVADKY
EEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHH
QVPMVKVTGGQRIDLLGIKKQDLPGVWKDLDMPSGHAYGKSIRTVKTCVGSEFCRFGTQN
CCCEEEECCCCEEEEEECCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
STQLGIDLEHDLFNMWSPHKVKLAVSGCPRNCSEAGIKDVGIIGVDSGWEMYIGGNGGIK
CCEEEEEECHHHHHCCCCCEEEEEECCCCCCCHHCCCCCEEEEEECCCCEEEECCCCCCH
TEVAEFFVKLKTAEEVREYNGAFLQLYREEAFYLERTVHYLQRVGMEHIKKAVLEDPERR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHH
KALNERLQFSLSFEQDPWKERLAQPQLKKEFDVLPVKNLEVPA
HHHHHHHEEEEECCCCHHHHHHCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLPENLMKKLKLVMIGNGMAGVRTLEELLKLSNELYDITVFGAEPHTNYNRILLSPVLAG
CCCHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEHHHCC
EQTFEEIVLNDLDWYLENNIKLLLNRKVVEIDRVKRRVIAEDGTEAEYDRLLIATGSTPF
HHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCE
ILPIPGNTLQGVIGYRDIADTQAMIDTAKTHKHAVVIGGGLLGLEAANGLMLRGMHVTVV
EEECCCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCEEEHHCCCCEEEECCEEEEH
HLGEWLLERQLDKTSGQLLQTALESRGLHFRLCEQTQALHDAGNGRVGSVQFKNGDIIPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCEEEEEECCCCCCCH
DLVVMAAGIRPNTELAEKAGIPCNRGILVNDTLQTYDPRIYAIGECASHRGIAYGLVAPL
HHHHEECCCCCCHHHHHHCCCCCCCCEEEECCHHHCCCEEEEEECHHCCCCCHHHHHHHH
FEQAKVCANHLAQLGFARYQGSVTSTKLKVTGIDLFSAGDFMGGEGTETITLSDPIGGVY
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCCCCCEEEEECCCCCHHH
KKLVIKDDVLVGACLYGDTADGGWYFRQIRENHAIGEIRDHLMFGENALGDVGHQGQDKA
HHHHEECCEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHCCCCCCCCCHH
MSMADNAEVCGCNGVCKGTIVKAIQEHGLFSVDEVKKHTKAASSCGSCAGLVEQILINTV
HHHCCCCCEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGAADVKPKSEKAICGCSDLNHGQIRQAIREQHLLTIAGTMSYLNWRTPNGCATCRPALN
CCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCCCCCCCC
YYLISTWPGEAKDDPQSRLINERAHANIQKDGTYSVVPRMWGGVTNPSELRRIADVADKY
EEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHH
QVPMVKVTGGQRIDLLGIKKQDLPGVWKDLDMPSGHAYGKSIRTVKTCVGSEFCRFGTQN
CCCEEEECCCCEEEEEECCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
STQLGIDLEHDLFNMWSPHKVKLAVSGCPRNCSEAGIKDVGIIGVDSGWEMYIGGNGGIK
CCEEEEEECHHHHHCCCCCEEEEEECCCCCCCHHCCCCCEEEEEECCCCEEEECCCCCCH
TEVAEFFVKLKTAEEVREYNGAFLQLYREEAFYLERTVHYLQRVGMEHIKKAVLEDPERR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHH
KALNERLQFSLSFEQDPWKERLAQPQLKKEFDVLPVKNLEVPA
HHHHHHHEEEEECCCCHHHHHHCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]