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The map label for this gene is rhtB [H]

Identifier: 77457937

GI number: 77457937

Start: 1905648

End: 1906277

Strand: Reverse

Name: rhtB [H]

Synonym: Pfl01_1710

Alternate gene names: 77457937

Gene position: 1906277-1905648 (Counterclockwise)

Preceding gene: 77457938

Following gene: 77457936

Centisome position: 29.61

GC content: 61.9

Gene sequence:

>630_bases
ATGAGTCTGGAAACCTGGCTGCTGTTCAGCGGCGCTGCGCTGGTAGTGATCCTGATTCCGGGGCCGCTGTCGTTGCTGAT
GATCAGCAACAGTCTGAACTACGGTCTGCGTCGTTCGTACCCGGCGTTTCTTGGCGGCGTGTTCGCCTCGATCTGCCTGC
TCAGCGCCTCGGCGCTGGGGTTGGGCGCCCTGTTGCTGGCGTCCGAGAAACTGTTCAGTGCCCTGAAAATCATCGGTGCG
ATCTACCTGTTCTACCTTGCCTGGCAGAGCTGGCAGCAATCGCGTCAGCCGTCGATGGGTGCCGAAGTGCCTCAGGCCGC
ACCGGTTCCGAAATTCCGCGCGCTGTTCAGCCGGGCCTTCGTGCTGGGCGCGAGCAATCCGAAAGACATTTTGTTTTTCG
CCGCGTTTCTGCCGCAATTTCTCAGCGCCGAGCAACCGTTCCTGCCGCAACTGCTGGTGATGATCGCCACCTGGACGCTG
CTCGATCTGCTGTGCAAATTGACCTACGGCCTCGGTGCCCATGGCGCGGCGCGTTATCTGCGCAGCGGCAAGGGGCAGAG
CTGGTTCAACCGCATCAGCGCCGGACTCTTCGGGGGGGCTGGTGCAGCTTCACTGCTGAGCAGCCACTGA

Upstream 100 bases:

>100_bases
CATCTTCCTGTAAGCGCTACGCGGTTCACGCTTCAAAACAGCCTCACCCCACCGGGTGGGGCTTTTGCACATTTTTGATA
ACGATAAAAAGGAGCAAGTG

Downstream 100 bases:

>100_bases
TCCGCGATCAGACTGACCGCCATTTCCAGGTGAAGTGGCGGTAGTTGTCCACCTGGAAACGTCCGGCATCAACTGTGTCA
CCCGATAGACATATGTACCG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSLETWLLFSGAALVVILIPGPLSLLMISNSLNYGLRRSYPAFLGGVFASICLLSASALGLGALLLASEKLFSALKIIGA
IYLFYLAWQSWQQSRQPSMGAEVPQAAPVPKFRALFSRAFVLGASNPKDILFFAAFLPQFLSAEQPFLPQLLVMIATWTL
LDLLCKLTYGLGAHGAARYLRSGKGQSWFNRISAGLFGGAGAASLLSSH

Sequences:

>Translated_209_residues
MSLETWLLFSGAALVVILIPGPLSLLMISNSLNYGLRRSYPAFLGGVFASICLLSASALGLGALLLASEKLFSALKIIGA
IYLFYLAWQSWQQSRQPSMGAEVPQAAPVPKFRALFSRAFVLGASNPKDILFFAAFLPQFLSAEQPFLPQLLVMIATWTL
LDLLCKLTYGLGAHGAARYLRSGKGQSWFNRISAGLFGGAGAASLLSSH
>Mature_208_residues
SLETWLLFSGAALVVILIPGPLSLLMISNSLNYGLRRSYPAFLGGVFASICLLSASALGLGALLLASEKLFSALKIIGAI
YLFYLAWQSWQQSRQPSMGAEVPQAAPVPKFRALFSRAFVLGASNPKDILFFAAFLPQFLSAEQPFLPQLLVMIATWTLL
DLLCKLTYGLGAHGAARYLRSGKGQSWFNRISAGLFGGAGAASLLSSH

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=206, Percent_Identity=32.0388349514563, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788099, Length=185, Percent_Identity=29.7297297297297, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22418; Mature: 22287

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLETWLLFSGAALVVILIPGPLSLLMISNSLNYGLRRSYPAFLGGVFASICLLSASALG
CCCCHHHEECCCEEEEEECCCCCEEHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHH
LGALLLASEKLFSALKIIGAIYLFYLAWQSWQQSRQPSMGAEVPQAAPVPKFRALFSRAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
VLGASNPKDILFFAAFLPQFLSAEQPFLPQLLVMIATWTLLDLLCKLTYGLGAHGAARYL
HHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RSGKGQSWFNRISAGLFGGAGAASLLSSH
HCCCCHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
SLETWLLFSGAALVVILIPGPLSLLMISNSLNYGLRRSYPAFLGGVFASICLLSASALG
CCCHHHEECCCEEEEEECCCCCEEHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHH
LGALLLASEKLFSALKIIGAIYLFYLAWQSWQQSRQPSMGAEVPQAAPVPKFRALFSRAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
VLGASNPKDILFFAAFLPQFLSAEQPFLPQLLVMIATWTLLDLLCKLTYGLGAHGAARYL
HHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RSGKGQSWFNRISAGLFGGAGAASLLSSH
HCCCCHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]