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The map label for this gene is purN [H]

Identifier: 77457859

GI number: 77457859

Start: 1823175

End: 1823825

Strand: Reverse

Name: purN [H]

Synonym: Pfl01_1632

Alternate gene names: 77457859

Gene position: 1823825-1823175 (Counterclockwise)

Preceding gene: 77457860

Following gene: 77457858

Centisome position: 28.33

GC content: 61.44

Gene sequence:

>651_bases
ATGTCCCAGACCTGTGATGTCGTGGTGCTGTTGTCCGGCACCGGCAGTAACTTGCAGGCCCTGATCGACAGCACGCGGAC
CGGCGACAGCCCGGTCCGCATCGCTGCGGTGATTTCCAACCGCGCCGACGCCTACGGCCTGCAACGCGCCAGTGACGCGG
GTATCGCCACCCGCTCGCTGGATCACAAGGGATTCGAGGGCCGCGAGGCCTTCGATACCGCTCTGATCGAACTGATCGAC
GAATTCAATCCCAAACTCGTGGTACTGGCCGGCTTCATGCGCATTCTCAGCGCTGACTTCGTCCGTCACTACCAGGGTCG
CCTGCTCAACATCCACCCGTCGCTACTCCCCAAATACAAAGGGTTACACACTCATCAGCGCGCGCTGGAAGCCGGCGACG
CCGAACACGGCTGCTCCGTGCACTTCGTCACCGAGGAACTCGATGGCGGACCACTGGTCGTACAGGCAGTAATACCGGTA
GAGTTGCACGATTCGCCGCAAAGTCTGGCGCAGCGGGTTCACGCTCAGGAACACCTGATCTACCCGATGGCCGTACGCTG
GTTCGCCGAAGGACGGCTGAGCCTCGGCGAGCAAGGTGCTTTACTGGATGGCCAGTTACTCGGGGCCAGCGGTCACTTGA
TTCGAAACTAG

Upstream 100 bases:

>100_bases
CCCTGCGTGACGCCGGCGAGCAGCCTTGGGTCATCGGTCAGATCGCTGCCGCTGCCGAAGGCGCGGCCCAGGTCGAACTG
AAGAACCTCAAGGCTCACTG

Downstream 100 bases:

>100_bases
GAGATTTTATGCGTCGCGCCCTGCTCTTCGCTTGCGCTCTGCTCGCCCTGCCTTTTGCCCAGGCGGCAGACCTTCAACCG
TTCTCCGCCAGCTACACTGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSQTCDVVVLLSGTGSNLQALIDSTRTGDSPVRIAAVISNRADAYGLQRASDAGIATRSLDHKGFEGREAFDTALIELID
EFNPKLVVLAGFMRILSADFVRHYQGRLLNIHPSLLPKYKGLHTHQRALEAGDAEHGCSVHFVTEELDGGPLVVQAVIPV
ELHDSPQSLAQRVHAQEHLIYPMAVRWFAEGRLSLGEQGALLDGQLLGASGHLIRN

Sequences:

>Translated_216_residues
MSQTCDVVVLLSGTGSNLQALIDSTRTGDSPVRIAAVISNRADAYGLQRASDAGIATRSLDHKGFEGREAFDTALIELID
EFNPKLVVLAGFMRILSADFVRHYQGRLLNIHPSLLPKYKGLHTHQRALEAGDAEHGCSVHFVTEELDGGPLVVQAVIPV
ELHDSPQSLAQRVHAQEHLIYPMAVRWFAEGRLSLGEQGALLDGQLLGASGHLIRN
>Mature_215_residues
SQTCDVVVLLSGTGSNLQALIDSTRTGDSPVRIAAVISNRADAYGLQRASDAGIATRSLDHKGFEGREAFDTALIELIDE
FNPKLVVLAGFMRILSADFVRHYQGRLLNIHPSLLPKYKGLHTHQRALEAGDAEHGCSVHFVTEELDGGPLVVQAVIPVE
LHDSPQSLAQRVHAQEHLIYPMAVRWFAEGRLSLGEQGALLDGQLLGASGHLIRN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=195, Percent_Identity=49.7435897435897, Blast_Score=201, Evalue=4e-52,
Organism=Homo sapiens, GI209869995, Length=195, Percent_Identity=49.7435897435897, Blast_Score=201, Evalue=4e-52,
Organism=Homo sapiens, GI209869993, Length=195, Percent_Identity=49.7435897435897, Blast_Score=201, Evalue=4e-52,
Organism=Escherichia coli, GI1788846, Length=207, Percent_Identity=59.4202898550725, Blast_Score=253, Evalue=5e-69,
Organism=Escherichia coli, GI1787483, Length=155, Percent_Identity=33.5483870967742, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=40.7821229050279, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6320616, Length=196, Percent_Identity=35.7142857142857, Blast_Score=102, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=50.8021390374332, Blast_Score=191, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23430; Mature: 23299

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTCDVVVLLSGTGSNLQALIDSTRTGDSPVRIAAVISNRADAYGLQRASDAGIATRSL
CCCCCCEEEEEECCCCCHHHHHHCCCCCCCCEEEEEEECCCCHHHCCCCCCCCCCEECCC
DHKGFEGREAFDTALIELIDEFNPKLVVLAGFMRILSADFVRHYQGRLLNIHPSLLPKYK
CCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECHHHCCHHC
GLHTHQRALEAGDAEHGCSVHFVTEELDGGPLVVQAVIPVELHDSPQSLAQRVHAQEHLI
CCHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHH
YPMAVRWFAEGRLSLGEQGALLDGQLLGASGHLIRN
HHHHHHHHHCCCCCCCCCCCEECCEEECCCCCCCCC
>Mature Secondary Structure 
SQTCDVVVLLSGTGSNLQALIDSTRTGDSPVRIAAVISNRADAYGLQRASDAGIATRSL
CCCCCEEEEEECCCCCHHHHHHCCCCCCCCEEEEEEECCCCHHHCCCCCCCCCCEECCC
DHKGFEGREAFDTALIELIDEFNPKLVVLAGFMRILSADFVRHYQGRLLNIHPSLLPKYK
CCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECHHHCCHHC
GLHTHQRALEAGDAEHGCSVHFVTEELDGGPLVVQAVIPVELHDSPQSLAQRVHAQEHLI
CCHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHH
YPMAVRWFAEGRLSLGEQGALLDGQLLGASGHLIRN
HHHHHHHHHCCCCCCCCCCCEECCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]