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The map label for this gene is flgJ [H]

Identifier: 77457732

GI number: 77457732

Start: 1683614

End: 1684906

Strand: Direct

Name: flgJ [H]

Synonym: Pfl01_1505

Alternate gene names: 77457732

Gene position: 1683614-1684906 (Clockwise)

Preceding gene: 77457731

Following gene: 77457733

Centisome position: 26.15

GC content: 61.41

Gene sequence:

>1293_bases
ATGGATATGCGCAAAAGCGGTCTGGTCAGCAGCAGCGATTCGGGTTCGTACTCCGACCTCAATCGCTTGAATCAGCTGAA
GGTCGGCGACAAGAACAGCGATGCGAACATGCGCAAGGTCGCGCAGGAATTCGAATCGCTGTTCCTCGGTGAAATGCTCA
AGTCGATGCGTTCGGCCACTGAAGCGCTGGGCCAGGACAACCCGCTCAATACGCCGGCCGCCAAGCAGTATCAGGAAATG
TATGACCAGCAACTGGCGGTTTCCCTGTCCCGCGAGGGCGGTGGTATTGGCCTGGCCGACGTGCTGATCAAGCAGATGTC
GAAGAACAAACCGATGGCGCCGGGCGAGGCTGCGGCCGCATCCGCCGCCAAGCAGGAAGAAGCCAGGGCCAAGGCCGCTG
CCGTGGCCACACCGATTGCCGCCGGTACCATGGCTGCCAATGGTCCGTTGTCGCGACTCAATGGCGAGCGTCCGCTGTGG
GCGTCGCGTTCGTTGCATGCGCCGAACACTGAACTGACCCATCGCAATGACATGGAAATGATCAACCAGCGTCGTCTGGC
GTTGCCGCCGAAACTGGCGGATCGCTTGCTCGCCGGTCTGGTGCCGTCGGCTACGCCGGCAGCGGCCACTCAACTGCCGC
AACGCGCCGCGACCGCCGCTGTTACCGGTTCCGGCCCGCTGTATAACGGCGACTGGCTGGCCCGCGCCGAGGCGGACAAG
GCTTCCGGCGGACAGATGCAGATTTACGGTCGTGCGATGGCACAGATCCCGTTGGCGCCAGCCAAGCGCGCGTTCAGCAA
CGCCGACCAGTTCGTCAACACGATGTTGCCGATGGCGCAGGAAGCCGCCGCGCGCATTGGCGTCGATCCGCGTTATCTGG
TGGCTCAGGCTGCGCTGGAAACCGGCTGGGGCAAATCGGTCATGCGCGCCCAGGACGGCAGCAGCAGTCACAACCTGTTC
GGCATCAAGGCCAGCAGCAACTGGAAGGGCGATTCGGCCCGCGCGATCACCAGCGAATTCCGCAACGGGCAGATGGTCAA
GGAGACGGCCGAGTTCCGTTCCTATGCCTCGTACAAGGACAGCTTCCACGATCTGGTGACTTTGCTGCAGAGCAATAATC
GCTATCAAGATGTGCTGAAGTCGGCCGATAACCCAGAACAGTTTGTACGCGAATTGCAGAAGGCCGGTTACGCGACCGAC
CCGAACTACGCGACGAAGATTTCGCAGATTGCCAGGCAGATGACGGTCAATCAGAACTACGCTGCGGCCGGCGTTTCAAC
AACGCCCTTATAA

Upstream 100 bases:

>100_bases
AGGTCGGCGCCGCGCCGGGTGACCTGATGGCCATCCTCGAAGCACTTAAGCAGGCCGGCGCGTTGCAAGCCGACCTGATC
GTGATCTGAGGACGGCGACC

Downstream 100 bases:

>100_bases
ACACAAGGTAAGGTTTGAATCATGAGTTTGCTCAATATCGGGATGTCGGGGTTGGCCGCGAGTTCATCCTCTCTGGCTGT
GACGGGCAACAACATTGCCA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MDMRKSGLVSSSDSGSYSDLNRLNQLKVGDKNSDANMRKVAQEFESLFLGEMLKSMRSATEALGQDNPLNTPAAKQYQEM
YDQQLAVSLSREGGGIGLADVLIKQMSKNKPMAPGEAAAASAAKQEEARAKAAAVATPIAAGTMAANGPLSRLNGERPLW
ASRSLHAPNTELTHRNDMEMINQRRLALPPKLADRLLAGLVPSATPAAATQLPQRAATAAVTGSGPLYNGDWLARAEADK
ASGGQMQIYGRAMAQIPLAPAKRAFSNADQFVNTMLPMAQEAAARIGVDPRYLVAQAALETGWGKSVMRAQDGSSSHNLF
GIKASSNWKGDSARAITSEFRNGQMVKETAEFRSYASYKDSFHDLVTLLQSNNRYQDVLKSADNPEQFVRELQKAGYATD
PNYATKISQIARQMTVNQNYAAAGVSTTPL

Sequences:

>Translated_430_residues
MDMRKSGLVSSSDSGSYSDLNRLNQLKVGDKNSDANMRKVAQEFESLFLGEMLKSMRSATEALGQDNPLNTPAAKQYQEM
YDQQLAVSLSREGGGIGLADVLIKQMSKNKPMAPGEAAAASAAKQEEARAKAAAVATPIAAGTMAANGPLSRLNGERPLW
ASRSLHAPNTELTHRNDMEMINQRRLALPPKLADRLLAGLVPSATPAAATQLPQRAATAAVTGSGPLYNGDWLARAEADK
ASGGQMQIYGRAMAQIPLAPAKRAFSNADQFVNTMLPMAQEAAARIGVDPRYLVAQAALETGWGKSVMRAQDGSSSHNLF
GIKASSNWKGDSARAITSEFRNGQMVKETAEFRSYASYKDSFHDLVTLLQSNNRYQDVLKSADNPEQFVRELQKAGYATD
PNYATKISQIARQMTVNQNYAAAGVSTTPL
>Mature_430_residues
MDMRKSGLVSSSDSGSYSDLNRLNQLKVGDKNSDANMRKVAQEFESLFLGEMLKSMRSATEALGQDNPLNTPAAKQYQEM
YDQQLAVSLSREGGGIGLADVLIKQMSKNKPMAPGEAAAASAAKQEEARAKAAAVATPIAAGTMAANGPLSRLNGERPLW
ASRSLHAPNTELTHRNDMEMINQRRLALPPKLADRLLAGLVPSATPAAATQLPQRAATAAVTGSGPLYNGDWLARAEADK
ASGGQMQIYGRAMAQIPLAPAKRAFSNADQFVNTMLPMAQEAAARIGVDPRYLVAQAALETGWGKSVMRAQDGSSSHNLF
GIKASSNWKGDSARAITSEFRNGQMVKETAEFRSYASYKDSFHDLVTLLQSNNRYQDVLKSADNPEQFVRELQKAGYATD
PNYATKISQIARQMTVNQNYAAAGVSTTPL

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG3951

COG function: function code MNO; Rod binding protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=139, Percent_Identity=47.4820143884892, Blast_Score=125, Evalue=7e-30,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR019301
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 46206; Mature: 46206

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMRKSGLVSSSDSGSYSDLNRLNQLKVGDKNSDANMRKVAQEFESLFLGEMLKSMRSAT
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EALGQDNPLNTPAAKQYQEMYDQQLAVSLSREGGGIGLADVLIKQMSKNKPMAPGEAAAA
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
SAAKQEEARAKAAAVATPIAAGTMAANGPLSRLNGERPLWASRSLHAPNTELTHRNDMEM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHH
INQRRLALPPKLADRLLAGLVPSATPAAATQLPQRAATAAVTGSGPLYNGDWLARAEADK
HHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCEEEECCCCC
ASGGQMQIYGRAMAQIPLAPAKRAFSNADQFVNTMLPMAQEAAARIGVDPRYLVAQAALE
CCCCEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TGWGKSVMRAQDGSSSHNLFGIKASSNWKGDSARAITSEFRNGQMVKETAEFRSYASYKD
HCCCHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SFHDLVTLLQSNNRYQDVLKSADNPEQFVRELQKAGYATDPNYATKISQIARQMTVNQNY
HHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
AAAGVSTTPL
CCCCCCCCCC
>Mature Secondary Structure
MDMRKSGLVSSSDSGSYSDLNRLNQLKVGDKNSDANMRKVAQEFESLFLGEMLKSMRSAT
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EALGQDNPLNTPAAKQYQEMYDQQLAVSLSREGGGIGLADVLIKQMSKNKPMAPGEAAAA
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
SAAKQEEARAKAAAVATPIAAGTMAANGPLSRLNGERPLWASRSLHAPNTELTHRNDMEM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHH
INQRRLALPPKLADRLLAGLVPSATPAAATQLPQRAATAAVTGSGPLYNGDWLARAEADK
HHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCEEEECCCCC
ASGGQMQIYGRAMAQIPLAPAKRAFSNADQFVNTMLPMAQEAAARIGVDPRYLVAQAALE
CCCCEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TGWGKSVMRAQDGSSSHNLFGIKASSNWKGDSARAITSEFRNGQMVKETAEFRSYASYKD
HCCCHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SFHDLVTLLQSNNRYQDVLKSADNPEQFVRELQKAGYATDPNYATKISQIARQMTVNQNY
HHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
AAAGVSTTPL
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]