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The map label for this gene is rluB [H]

Identifier: 77457719

GI number: 77457719

Start: 1669825

End: 1671042

Strand: Direct

Name: rluB [H]

Synonym: Pfl01_1492

Alternate gene names: 77457719

Gene position: 1669825-1671042 (Clockwise)

Preceding gene: 77457718

Following gene: 77457720

Centisome position: 25.94

GC content: 63.88

Gene sequence:

>1218_bases
ATGAGTGACATCAATCAGAAAGACGACCAGGAAATCGGCCCAGCAGGCGAAAAGCTGCAGAAAGTCCTCGCCCGTATCGG
CGTCGGCTCGCGCCGTGACGTCGAATCCTGGATCGCTGCCGGCCGGATCAAGGTCAATGGCAAAGACGCCACTTTGGGCC
TGCGTGTCGACATGCACGATGCAATCACCATTGATGGCAAGGTCATCAAGCGCGAAGAAGCCGCCGAGTCGGTTCGCCGC
GTGATCATGTACAACAAGCCCGATGGCGAGATCTGCACCCGTGACGACCCGGAAGGCCGTCCGACCGTGTTCGACAAGCT
GCCGCGTCCGAAAGAAGGCCGCTGGATCAACATCGGTCGTCTCGACATCAACACCACCGGTCTGCTGATGTTCACTACCG
ACGGTGAACTGGCCAACCGCCTAATGCACCCGTCCTACGAGATGGACCGTGAATACGCGGTGCGTGTCCGCGGTGAAGTC
GATGACGAAATGATCGAGCGCCTGAAGGCCGGCGTCGTGCTGGAAGACGGCCCGGCGCGTTTCACCGACATCCAACAGGC
ACCGGGTGGCGAAGGCTTCAACCACTGGTATCACTGCGTGGTGATGGAAGGCCGTAACCGTGAAGTACGTCGCCTTTGGG
AATCCCAGGGCCTGGTGGTCAGCCGCCTGAAGCGCGTGCGTTTCGGTCCGGTGTTCCTCAACTCCGACCTGCCGATGGGC
CGCTGGCGCGAAATGAGCCAGTACGAAGTTGACGTGCTGAGCGCCGAAGTCGGCCTGACTCCGGTGGCCATGCCGCAACT
GAATGCCAAGAGCAAAGACAAGCTCGACCGCATGCAGCGCAAGTCGTCGCGTCCGATGGCCCGTACCGAGCGTGTGCGCA
CCCTGCGTCCGGCCAACGGTGCACCGATTGGTGCCGGTCCGCGTCCGGTTCGCGAGCCGCAGATCGAAGGCGAGCGTCCA
GGTCGCAAGCCGGCAGCGCGTCCGGATGGCGAGCGTGGGCCACGCAGTCCGCGTCCGGCCAATGGCCGTACCGAGCGTGG
CGAAGGTCGTGGTACGCCGGTGGCGGATCGTCCAGTCGACACCAAGCGCCCGGCCAAACCGGCGCCGAAGCGCCCGGGCA
TCAAGCTGGCCGACGATGACAAGCCGTCGGGCAAGCGCCGTGGCGCACCAGCCGGTTCCGGCCAGCGTCCGGGGTTTGGT
CGCAAGAAGCCGGAATAA

Upstream 100 bases:

>100_bases
TCGCGACCCTTCGGCGATCCCTTCGCCCGAAAACCCAGATTTCAACGTTTCAGCGGCTCAATTCAGCGCCGCTGAACAGA
CCACACCGGGAGGTGCCCAG

Downstream 100 bases:

>100_bases
GACGGTTGTAAGCTTCAAGCTTCAAGCTGAAAACAGAAACGCCAACCTCAGGGTTGGCGTTTTTTTTTGGACTTTTTATT
TCTTTTTTCGGGTGTGTTTT

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSDINQKDDQEIGPAGEKLQKVLARIGVGSRRDVESWIAAGRIKVNGKDATLGLRVDMHDAITIDGKVIKREEAAESVRR
VIMYNKPDGEICTRDDPEGRPTVFDKLPRPKEGRWINIGRLDINTTGLLMFTTDGELANRLMHPSYEMDREYAVRVRGEV
DDEMIERLKAGVVLEDGPARFTDIQQAPGGEGFNHWYHCVVMEGRNREVRRLWESQGLVVSRLKRVRFGPVFLNSDLPMG
RWREMSQYEVDVLSAEVGLTPVAMPQLNAKSKDKLDRMQRKSSRPMARTERVRTLRPANGAPIGAGPRPVREPQIEGERP
GRKPAARPDGERGPRSPRPANGRTERGEGRGTPVADRPVDTKRPAKPAPKRPGIKLADDDKPSGKRRGAPAGSGQRPGFG
RKKPE

Sequences:

>Translated_405_residues
MSDINQKDDQEIGPAGEKLQKVLARIGVGSRRDVESWIAAGRIKVNGKDATLGLRVDMHDAITIDGKVIKREEAAESVRR
VIMYNKPDGEICTRDDPEGRPTVFDKLPRPKEGRWINIGRLDINTTGLLMFTTDGELANRLMHPSYEMDREYAVRVRGEV
DDEMIERLKAGVVLEDGPARFTDIQQAPGGEGFNHWYHCVVMEGRNREVRRLWESQGLVVSRLKRVRFGPVFLNSDLPMG
RWREMSQYEVDVLSAEVGLTPVAMPQLNAKSKDKLDRMQRKSSRPMARTERVRTLRPANGAPIGAGPRPVREPQIEGERP
GRKPAARPDGERGPRSPRPANGRTERGEGRGTPVADRPVDTKRPAKPAPKRPGIKLADDDKPSGKRRGAPAGSGQRPGFG
RKKPE
>Mature_404_residues
SDINQKDDQEIGPAGEKLQKVLARIGVGSRRDVESWIAAGRIKVNGKDATLGLRVDMHDAITIDGKVIKREEAAESVRRV
IMYNKPDGEICTRDDPEGRPTVFDKLPRPKEGRWINIGRLDINTTGLLMFTTDGELANRLMHPSYEMDREYAVRVRGEVD
DEMIERLKAGVVLEDGPARFTDIQQAPGGEGFNHWYHCVVMEGRNREVRRLWESQGLVVSRLKRVRFGPVFLNSDLPMGR
WREMSQYEVDVLSAEVGLTPVAMPQLNAKSKDKLDRMQRKSSRPMARTERVRTLRPANGAPIGAGPRPVREPQIEGERPG
RKPAARPDGERGPRSPRPANGRTERGEGRGTPVADRPVDTKRPAKPAPKRPGIKLADDDKPSGKRRGAPAGSGQRPGFGR
KKPE

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=59.1093117408907, Blast_Score=281, Evalue=7e-77,
Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=32.0512820512821, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788510, Length=176, Percent_Identity=32.3863636363636, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081838, Length=188, Percent_Identity=31.3829787234043, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 45195; Mature: 45064

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDINQKDDQEIGPAGEKLQKVLARIGVGSRRDVESWIAAGRIKVNGKDATLGLRVDMHD
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCEEEECCCCEEEEEEEECCC
AITIDGKVIKREEAAESVRRVIMYNKPDGEICTRDDPEGRPTVFDKLPRPKEGRWINIGR
EEEECCCEECHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEE
LDINTTGLLMFTTDGELANRLMHPSYEMDREYAVRVRGEVDDEMIERLKAGVVLEDGPAR
EEECCCEEEEEECCHHHHHHHCCCCHHCCCEEEEEECCCCCHHHHHHHHCCEEEECCCCC
FTDIQQAPGGEGFNHWYHCVVMEGRNREVRRLWESQGLVVSRLKRVRFGPVFLNSDLPMG
HHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCHH
RWREMSQYEVDVLSAEVGLTPVAMPQLNAKSKDKLDRMQRKSSRPMARTERVRTLRPANG
HHHHCHHHEEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
APIGAGPRPVREPQIEGERPGRKPAARPDGERGPRSPRPANGRTERGEGRGTPVADRPVD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TKRPAKPAPKRPGIKLADDDKPSGKRRGAPAGSGQRPGFGRKKPE
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDINQKDDQEIGPAGEKLQKVLARIGVGSRRDVESWIAAGRIKVNGKDATLGLRVDMHD
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCEEEECCCCEEEEEEEECCC
AITIDGKVIKREEAAESVRRVIMYNKPDGEICTRDDPEGRPTVFDKLPRPKEGRWINIGR
EEEECCCEECHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEE
LDINTTGLLMFTTDGELANRLMHPSYEMDREYAVRVRGEVDDEMIERLKAGVVLEDGPAR
EEECCCEEEEEECCHHHHHHHCCCCHHCCCEEEEEECCCCCHHHHHHHHCCEEEECCCCC
FTDIQQAPGGEGFNHWYHCVVMEGRNREVRRLWESQGLVVSRLKRVRFGPVFLNSDLPMG
HHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCHH
RWREMSQYEVDVLSAEVGLTPVAMPQLNAKSKDKLDRMQRKSSRPMARTERVRTLRPANG
HHHHCHHHEEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
APIGAGPRPVREPQIEGERPGRKPAARPDGERGPRSPRPANGRTERGEGRGTPVADRPVD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TKRPAKPAPKRPGIKLADDDKPSGKRRGAPAGSGQRPGFGRKKPE
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]