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The map label for this gene is yneJ [H]

Identifier: 77457677

GI number: 77457677

Start: 1622679

End: 1623572

Strand: Direct

Name: yneJ [H]

Synonym: Pfl01_1450

Alternate gene names: 77457677

Gene position: 1622679-1623572 (Clockwise)

Preceding gene: 77457675

Following gene: 77457685

Centisome position: 25.2

GC content: 65.88

Gene sequence:

>894_bases
GTGGAGTTCAGCCAATTGCGGATCTTCCAGGCCGTGGCGGAGGAGGGCTCGATCACCCGCGCCGCCGAACGTTTGCATCG
CGTGCCGTCGAACCTGTCGACCCGGCTCAAACAGCTGGAGGAGCAACTGGGTGTCGAACTGTTCGTCCGTGAGCGTCAGC
GTTTGCAGTTGTCGCCTGCGGGAAAAGTCCTGCTGGACTACACCGGCAAACTGTTCGCCCTGCGCGATCAGGCCAGTGCG
GCGGTGATGGGCGGGCAACCGGCGGGGGATTTCGTGCTCGGCACGCTGTACAGCACCGCCGCGATCCATTTGCCGGCGCT
GCTCGCGCGGTATCACAAGCAATATCCGGCGGTGAATCTTCAGGTGCAGTCCGGGCCCAGTGGTGAATTGCTTGAAGGCC
TGCTCACCGGTCGTCTCGACGCCGCGCTGGTGGATGGCCCGCCGCAACTGGCCGGGATCGACGGTGTGCCGCTGTGCAAC
GAGCGGCTGGTGCTGATCAGCGAGGCCGATCACCCGCCGATACGCAGCGCCAGGGATGTCGAGGGGCGGGCGGTTTTCAC
CTTCCGTCATGGCTGCGCCTATCGCGCGCGGCTGGAAGCGTGGTTCGCCCACTATCAGGCGGCGATGGGCCGGGCGATGG
AGATCGAGTCCTATCAGGGCATGCTCGCCTGCGTGATTGCGGGCAGCGGTGTGGCGCTGATGTCGGAGTCGATGCTCGCC
AGCCTGCCGGGGCGCGAAAGCGTGGCGGTGCACCCGCTGGCCGAGCCTTTTGCCAGTGCGACAACATGGCTCATGTGGCG
CCGGGGCATGGTCGGTGCCAACCTCAATGCGTGGATCGAACAGCAGCAGGCGCTCTATCCCGTGGCTGGCGAGGACGTGC
GGGAAACGGCTTGA

Upstream 100 bases:

>100_bases
TGAATTCCGTTGCAGGTTCAATGACCGTCAGGCTAGGACTGATTGACTTTCTTTAAAATTGAATAATAGTGAGTAACTTG
TTCAGTTCAGGAGAAAGGTT

Downstream 100 bases:

>100_bases
ACGAACGGTCAGGGATTTGGATCAATTCAGTAACAGATCATTGCGGATTTGGCCGAGCATTGCGTAGGACTTCGGACTAT
TATCAGTGCGAAGCGGCCTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MEFSQLRIFQAVAEEGSITRAAERLHRVPSNLSTRLKQLEEQLGVELFVRERQRLQLSPAGKVLLDYTGKLFALRDQASA
AVMGGQPAGDFVLGTLYSTAAIHLPALLARYHKQYPAVNLQVQSGPSGELLEGLLTGRLDAALVDGPPQLAGIDGVPLCN
ERLVLISEADHPPIRSARDVEGRAVFTFRHGCAYRARLEAWFAHYQAAMGRAMEIESYQGMLACVIAGSGVALMSESMLA
SLPGRESVAVHPLAEPFASATTWLMWRRGMVGANLNAWIEQQQALYPVAGEDVRETA

Sequences:

>Translated_297_residues
MEFSQLRIFQAVAEEGSITRAAERLHRVPSNLSTRLKQLEEQLGVELFVRERQRLQLSPAGKVLLDYTGKLFALRDQASA
AVMGGQPAGDFVLGTLYSTAAIHLPALLARYHKQYPAVNLQVQSGPSGELLEGLLTGRLDAALVDGPPQLAGIDGVPLCN
ERLVLISEADHPPIRSARDVEGRAVFTFRHGCAYRARLEAWFAHYQAAMGRAMEIESYQGMLACVIAGSGVALMSESMLA
SLPGRESVAVHPLAEPFASATTWLMWRRGMVGANLNAWIEQQQALYPVAGEDVRETA
>Mature_297_residues
MEFSQLRIFQAVAEEGSITRAAERLHRVPSNLSTRLKQLEEQLGVELFVRERQRLQLSPAGKVLLDYTGKLFALRDQASA
AVMGGQPAGDFVLGTLYSTAAIHLPALLARYHKQYPAVNLQVQSGPSGELLEGLLTGRLDAALVDGPPQLAGIDGVPLCN
ERLVLISEADHPPIRSARDVEGRAVFTFRHGCAYRARLEAWFAHYQAAMGRAMEIESYQGMLACVIAGSGVALMSESMLA
SLPGRESVAVHPLAEPFASATTWLMWRRGMVGANLNAWIEQQQALYPVAGEDVRETA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=280, Percent_Identity=49.6428571428571, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=27.8884462151394, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI145693105, Length=306, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788297, Length=253, Percent_Identity=28.8537549407115, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=27.1477663230241, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790262, Length=259, Percent_Identity=27.027027027027, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=173, Percent_Identity=30.0578034682081, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790399, Length=280, Percent_Identity=24.6428571428571, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788481, Length=242, Percent_Identity=25.2066115702479, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787879, Length=275, Percent_Identity=26.9090909090909, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788296, Length=289, Percent_Identity=23.5294117647059, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI87081904, Length=176, Percent_Identity=29.5454545454545, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789204, Length=241, Percent_Identity=25.7261410788382, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=240, Percent_Identity=24.5833333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=277, Percent_Identity=23.826714801444, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786713, Length=174, Percent_Identity=31.6091954022989, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788748, Length=173, Percent_Identity=27.1676300578035, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788706, Length=124, Percent_Identity=29.8387096774194, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32350; Mature: 32350

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFSQLRIFQAVAEEGSITRAAERLHRVPSNLSTRLKQLEEQLGVELFVRERQRLQLSPA
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHEECCCC
GKVLLDYTGKLFALRDQASAAVMGGQPAGDFVLGTLYSTAAIHLPALLARYHKQYPAVNL
CCEEEECCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
QVQSGPSGELLEGLLTGRLDAALVDGPPQLAGIDGVPLCNERLVLISEADHPPIRSARDV
EECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
EGRAVFTFRHGCAYRARLEAWFAHYQAAMGRAMEIESYQGMLACVIAGSGVALMSESMLA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEHHHHCCEEEEEEECCCHHHHHHHHHH
SLPGRESVAVHPLAEPFASATTWLMWRRGMVGANLNAWIEQQQALYPVAGEDVRETA
HCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure
MEFSQLRIFQAVAEEGSITRAAERLHRVPSNLSTRLKQLEEQLGVELFVRERQRLQLSPA
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHEECCCC
GKVLLDYTGKLFALRDQASAAVMGGQPAGDFVLGTLYSTAAIHLPALLARYHKQYPAVNL
CCEEEECCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
QVQSGPSGELLEGLLTGRLDAALVDGPPQLAGIDGVPLCNERLVLISEADHPPIRSARDV
EECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
EGRAVFTFRHGCAYRARLEAWFAHYQAAMGRAMEIESYQGMLACVIAGSGVALMSESMLA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEHHHHCCEEEEEEECCCHHHHHHHHHH
SLPGRESVAVHPLAEPFASATTWLMWRRGMVGANLNAWIEQQQALYPVAGEDVRETA
HCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]