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The map label for this gene is mscM [H]

Identifier: 77457663

GI number: 77457663

Start: 1610611

End: 1611909

Strand: Reverse

Name: mscM [H]

Synonym: Pfl01_1436

Alternate gene names: 77457663

Gene position: 1611909-1610611 (Counterclockwise)

Preceding gene: 77457665

Following gene: 77457661

Centisome position: 25.04

GC content: 60.97

Gene sequence:

>1299_bases
ATGGATTTCAAACAGCTCTGGCTCAACGCCCAAGACCTCTGGGGTGCCCTCGAACAACACCCGCTCCTGCACGCCGGCCT
GGCACTGCTCCTGTTATTGGTGATCGCGCTGGTACTCGGACGAGTGGCGCGTTATCTGATCCTGCATGCCAGCCGCATGC
TCGGTCGCCAGCCCGCACTGCACTGGATCAACGACTTTCGCCATAACAAGGTGTTTCAGCGTCTGGCGCAGATGACGCCG
TCGCTGGTGATCCAGTTCGGCCTGCACCTGGTGCCGGAGCTGAGCAAGACTGCCACGGTGTTCCTCGGCAATGTGGCGCT
GGCATTTACCATTCTGTTCCTGCTGCTGTCGGTCAGCGCCCTGCTCAATGCGCTGCTGGACATCTACGCCCGCACCGAAC
ACGCCCGCACCCGCTCGATCAAGGGGTATGTGCAACTGGCGAAAATGGTCCTGTACGTGTTCGGCGCGATCATCATCGTC
GCCACCCTGATCGATCGTTCGCCGTTGTTGCTGCTGTCCGGTCTCGGTGCGATGTCGGCGGTGATCCTGTTGGTCTACAA
GGACACCCTGCTGTCGTTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCGGGTCGGCGACTGGATCGAGATGC
CGCAAGTCGGCGCCGATGGCGACGTGGTCGACATCACTTTGCACACGGTCAAGGTACAGAACTTCGACAAAACCATCGTC
TCGATCCCGACCTGGCGCCTGATGTCCGAGTCGTTCCGCAACTGGCGCGGCATGCAGCAGTCCGGCGGGCGACGGATCAA
GCGCAGTCTGTTCATCGACGCCAGCGGAGTGCGCTTCATCCGCGACGATGAAGAAGAAAAACTCTCCCAGGTGCACCTGC
TGACCGCGTACATGGGCCGCAAGAAAGCCGAGCTCAAGGCCTGGAACGAAGCGCAGGGCAACGTCGCGGCGATGTCGGCC
AACCGCCGGCGCATGACCAACATCGGCACCTTCCGCGCTTACGCGCTGGCCTATCTGAAGAGTCACCCGGAGATCCAGCC
GAACATGACCTGCATGGTCCGCCAGATGCAAACCACCGCCCAGGGCATTCCGCTGGAAATCTACTGCTTTACCACCACCA
CGGTGTGGGCCGATTACGAGCGGATCCAGGGGGATATTTTCGATTACCTGCTGGCGGTGCTGCCGGAGTTCGGGCTGAGC
CTGTATCAGCAGCCGAGCGGTGGGGATTTGCGGTCAGGGTTGTTGCCGGCAGTCCTTGGCGCGAGCCACATTCCAGAAGC
CGAAAAACATCTGATGTAG

Upstream 100 bases:

>100_bases
TCAGTGGCTTTTTTGTTGCTCGATTTGTCTCCAACGGTAATACACAGCGTCTTAAATGCTCTAATCGCCCGTCGCGTCTT
ACAGAAGAATCGTTTCACTC

Downstream 100 bases:

>100_bases
CGCTTTCTTCAAATGCAAAAAAAGACGCAACCTTGGTTGCGTCTTTTTTTCCCGCCGATTTATCAGGCCTGCCGCTGCGG
TGCCTTGTGCACCATGGTGT

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MDFKQLWLNAQDLWGALEQHPLLHAGLALLLLLVIALVLGRVARYLILHASRMLGRQPALHWINDFRHNKVFQRLAQMTP
SLVIQFGLHLVPELSKTATVFLGNVALAFTILFLLLSVSALLNALLDIYARTEHARTRSIKGYVQLAKMVLYVFGAIIIV
ATLIDRSPLLLLSGLGAMSAVILLVYKDTLLSFVASVQLTSNDMLRVGDWIEMPQVGADGDVVDITLHTVKVQNFDKTIV
SIPTWRLMSESFRNWRGMQQSGGRRIKRSLFIDASGVRFIRDDEEEKLSQVHLLTAYMGRKKAELKAWNEAQGNVAAMSA
NRRRMTNIGTFRAYALAYLKSHPEIQPNMTCMVRQMQTTAQGIPLEIYCFTTTTVWADYERIQGDIFDYLLAVLPEFGLS
LYQQPSGGDLRSGLLPAVLGASHIPEAEKHLM

Sequences:

>Translated_432_residues
MDFKQLWLNAQDLWGALEQHPLLHAGLALLLLLVIALVLGRVARYLILHASRMLGRQPALHWINDFRHNKVFQRLAQMTP
SLVIQFGLHLVPELSKTATVFLGNVALAFTILFLLLSVSALLNALLDIYARTEHARTRSIKGYVQLAKMVLYVFGAIIIV
ATLIDRSPLLLLSGLGAMSAVILLVYKDTLLSFVASVQLTSNDMLRVGDWIEMPQVGADGDVVDITLHTVKVQNFDKTIV
SIPTWRLMSESFRNWRGMQQSGGRRIKRSLFIDASGVRFIRDDEEEKLSQVHLLTAYMGRKKAELKAWNEAQGNVAAMSA
NRRRMTNIGTFRAYALAYLKSHPEIQPNMTCMVRQMQTTAQGIPLEIYCFTTTTVWADYERIQGDIFDYLLAVLPEFGLS
LYQQPSGGDLRSGLLPAVLGASHIPEAEKHLM
>Mature_432_residues
MDFKQLWLNAQDLWGALEQHPLLHAGLALLLLLVIALVLGRVARYLILHASRMLGRQPALHWINDFRHNKVFQRLAQMTP
SLVIQFGLHLVPELSKTATVFLGNVALAFTILFLLLSVSALLNALLDIYARTEHARTRSIKGYVQLAKMVLYVFGAIIIV
ATLIDRSPLLLLSGLGAMSAVILLVYKDTLLSFVASVQLTSNDMLRVGDWIEMPQVGADGDVVDITLHTVKVQNFDKTIV
SIPTWRLMSESFRNWRGMQQSGGRRIKRSLFIDASGVRFIRDDEEEKLSQVHLLTAYMGRKKAELKAWNEAQGNVAAMSA
NRRRMTNIGTFRAYALAYLKSHPEIQPNMTCMVRQMQTTAQGIPLEIYCFTTTTVWADYERIQGDIFDYLLAVLPEFGLS
LYQQPSGGDLRSGLLPAVLGASHIPEAEKHLM

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=382, Percent_Identity=43.455497382199, Blast_Score=343, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 48457; Mature: 48457

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKQLWLNAQDLWGALEQHPLLHAGLALLLLLVIALVLGRVARYLILHASRMLGRQPAL
CCHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
HWINDFRHNKVFQRLAQMTPSLVIQFGLHLVPELSKTATVFLGNVALAFTILFLLLSVSA
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNALLDIYARTEHARTRSIKGYVQLAKMVLYVFGAIIIVATLIDRSPLLLLSGLGAMSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VILLVYKDTLLSFVASVQLTSNDMLRVGDWIEMPQVGADGDVVDITLHTVKVQNFDKTIV
HHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCCHHH
SIPTWRLMSESFRNWRGMQQSGGRRIKRSLFIDASGVRFIRDDEEEKLSQVHLLTAYMGR
CCCHHHHHHHHHHHHCCHHHCCCHHHHHHHEECCCCCEEECCCHHHHHHHHHHHHHHHCC
KKAELKAWNEAQGNVAAMSANRRRMTNIGTFRAYALAYLKSHPEIQPNMTCMVRQMQTTA
CHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QGIPLEIYCFTTTTVWADYERIQGDIFDYLLAVLPEFGLSLYQQPSGGDLRSGLLPAVLG
CCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
ASHIPEAEKHLM
CCCCCCHHHHCC
>Mature Secondary Structure
MDFKQLWLNAQDLWGALEQHPLLHAGLALLLLLVIALVLGRVARYLILHASRMLGRQPAL
CCHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
HWINDFRHNKVFQRLAQMTPSLVIQFGLHLVPELSKTATVFLGNVALAFTILFLLLSVSA
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNALLDIYARTEHARTRSIKGYVQLAKMVLYVFGAIIIVATLIDRSPLLLLSGLGAMSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VILLVYKDTLLSFVASVQLTSNDMLRVGDWIEMPQVGADGDVVDITLHTVKVQNFDKTIV
HHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCCHHH
SIPTWRLMSESFRNWRGMQQSGGRRIKRSLFIDASGVRFIRDDEEEKLSQVHLLTAYMGR
CCCHHHHHHHHHHHHCCHHHCCCHHHHHHHEECCCCCEEECCCHHHHHHHHHHHHHHHCC
KKAELKAWNEAQGNVAAMSANRRRMTNIGTFRAYALAYLKSHPEIQPNMTCMVRQMQTTA
CHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QGIPLEIYCFTTTTVWADYERIQGDIFDYLLAVLPEFGLSLYQQPSGGDLRSGLLPAVLG
CCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
ASHIPEAEKHLM
CCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]