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The map label for this gene is nadB [H]

Identifier: 77457587

GI number: 77457587

Start: 1533967

End: 1535583

Strand: Reverse

Name: nadB [H]

Synonym: Pfl01_1360

Alternate gene names: 77457587

Gene position: 1535583-1533967 (Counterclockwise)

Preceding gene: 77457588

Following gene: 77457584

Centisome position: 23.85

GC content: 61.72

Gene sequence:

>1617_bases
ATGAGCCAACAGTTTCAACACGATGTTCTGGTGATTGGCAGCGGCGCTGCCGGTTTGAGTCTCGCGCTGACCCTGCCCGG
TCACTTGCGCATCGCCGTACTGAGCAAAGGCGATCTTGCCAACGGCTCGACCTATTGGGCACAGGGTGGCGTCGCAGCCG
TGCTGGACGACACCGACACTGTCGAATCCCATGTCGATGACACCCTCAATGCTGGCGGCGGTTTGTGCCATGAAGACGCG
GTGCGTTTCACCGTCGAGCACAGCAAGGAAGCGATCCAATGGCTGATCGACCAGGGCGTGCCGTTCACTCGCGACGAACA
GTCCGGCACCGAAGACGGCGGCTTCGAATTTCACCTCACCCGCGAAGGCGGTCACAGCCATCGACGGATCATCCACGCCG
CCGATGCCACTGGCGCTGCGATCTTCACGACCCTGCTGGCCCAGGCCAAAGAACGCCCGAACATCGAACTGCTCGAACAG
CGGGTAGCGGTCGATCTGATCACCGAGCGTCGGCTGGGGCTGGAAGGCGAACGCTGCCTCGGCGCCTACGTACTCAACCG
CAAAACCGGGGAAGTCGATACCTTCGGTGCACGCTTCGTGATCCTGGCGTCCGGCGGCGCCGCGAAAGTCTATCTATATA
CAAGCAACCCCGACGGCGCCTGTGGTGACGGCATCGCCATGGCCTGGCGCTCGGGCTGCCGAGTGGCGAACCTGGAGTTC
AACCAGTTCCACCCCACCTGCCTCTACCACCCGCAAGCCAAGAGTTTTCTGGTAACCGAAGCCCTGCGCGGTGAAGGTGC
CCATTTGAAGCTGCCGAACGGCGAACGCTTCATGCAGCGCTTCGACTCACGCGCAGAACTGGCACCACGGGACATCGTCG
CCCGCGCCATCGACCACGAAATGAAGCGCCTGGGTGTCGATTGCGTCTACCTCGACATCAGCCACAAACCCGAAGCCTTC
ATCAAATCGCACTTCCCGACCGTCTACGAACGCTGCCTCGGATTCGGCATCGACATCACCAAGCAACCGATCCCGGTCGT
ACCGGCTGCGCATTACACCTGCGGCGGAGTGATGGTCGATCAACAGGGCCGCACCGATGTGCCCGGCCTGTACGCCATTG
GCGAAACCAGCTTCACCGGCCTGCATGGCGCCAACCGCATGGCCAGCAACTCGCTGCTGGAATGCTTCGTTTACGCCCGA
TCGGCAGCGGCGGACATTCTCGAGCAACTGCCCGACGTCTTGATCCCGCGCGCCCTGCCCGCCTGGGACGCCAGCCAGGT
GACCGACTCCGATGAAGACGTGATCATCGCCCACAACTGGGACGAATTGCGGCGCTTCATGTGGGACTACGTGGGCATCG
TGCGCACCAACAAGCGCCTGCAAAGGGCACAACACCGCGTGCGTCTGCTGCTGGATGAAATCGACGAGTTCTACAGCAAC
TATAAAGTCAGTCGCGACCTGATCGAGTTGCGCAACCTGGCGCAAGTAGCGGAACTGATGATCCGCTCGGCCATGGAGCG
CAAGGAAAGCCGAGGCCTGCATTACACACTCGACTACCCAGACCTGCTGCCCGAAGCACTCGACACTATTCTGGTGCCGC
CCACCTACGTCGGCTGA

Upstream 100 bases:

>100_bases
CACCCCTCTATATACTAGTGGGCTGTGTGGCTGTCTCGACTCGCCCATCCGCCTGTCCTGCGCCAACCCCGACCCGGGTC
GCTTTGAGCGGAATCCTGAA

Downstream 100 bases:

>100_bases
ACTTGAGGCGTACCCGCAGGCGTCGGTGCAGATCCGCCGCCTGCGAGTCCCGCGTTACACAGATCGACCTGACCCGCCGT
TCACCACGCAATCTAAAGCG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MSQQFQHDVLVIGSGAAGLSLALTLPGHLRIAVLSKGDLANGSTYWAQGGVAAVLDDTDTVESHVDDTLNAGGGLCHEDA
VRFTVEHSKEAIQWLIDQGVPFTRDEQSGTEDGGFEFHLTREGGHSHRRIIHAADATGAAIFTTLLAQAKERPNIELLEQ
RVAVDLITERRLGLEGERCLGAYVLNRKTGEVDTFGARFVILASGGAAKVYLYTSNPDGACGDGIAMAWRSGCRVANLEF
NQFHPTCLYHPQAKSFLVTEALRGEGAHLKLPNGERFMQRFDSRAELAPRDIVARAIDHEMKRLGVDCVYLDISHKPEAF
IKSHFPTVYERCLGFGIDITKQPIPVVPAAHYTCGGVMVDQQGRTDVPGLYAIGETSFTGLHGANRMASNSLLECFVYAR
SAAADILEQLPDVLIPRALPAWDASQVTDSDEDVIIAHNWDELRRFMWDYVGIVRTNKRLQRAQHRVRLLLDEIDEFYSN
YKVSRDLIELRNLAQVAELMIRSAMERKESRGLHYTLDYPDLLPEALDTILVPPTYVG

Sequences:

>Translated_538_residues
MSQQFQHDVLVIGSGAAGLSLALTLPGHLRIAVLSKGDLANGSTYWAQGGVAAVLDDTDTVESHVDDTLNAGGGLCHEDA
VRFTVEHSKEAIQWLIDQGVPFTRDEQSGTEDGGFEFHLTREGGHSHRRIIHAADATGAAIFTTLLAQAKERPNIELLEQ
RVAVDLITERRLGLEGERCLGAYVLNRKTGEVDTFGARFVILASGGAAKVYLYTSNPDGACGDGIAMAWRSGCRVANLEF
NQFHPTCLYHPQAKSFLVTEALRGEGAHLKLPNGERFMQRFDSRAELAPRDIVARAIDHEMKRLGVDCVYLDISHKPEAF
IKSHFPTVYERCLGFGIDITKQPIPVVPAAHYTCGGVMVDQQGRTDVPGLYAIGETSFTGLHGANRMASNSLLECFVYAR
SAAADILEQLPDVLIPRALPAWDASQVTDSDEDVIIAHNWDELRRFMWDYVGIVRTNKRLQRAQHRVRLLLDEIDEFYSN
YKVSRDLIELRNLAQVAELMIRSAMERKESRGLHYTLDYPDLLPEALDTILVPPTYVG
>Mature_537_residues
SQQFQHDVLVIGSGAAGLSLALTLPGHLRIAVLSKGDLANGSTYWAQGGVAAVLDDTDTVESHVDDTLNAGGGLCHEDAV
RFTVEHSKEAIQWLIDQGVPFTRDEQSGTEDGGFEFHLTREGGHSHRRIIHAADATGAAIFTTLLAQAKERPNIELLEQR
VAVDLITERRLGLEGERCLGAYVLNRKTGEVDTFGARFVILASGGAAKVYLYTSNPDGACGDGIAMAWRSGCRVANLEFN
QFHPTCLYHPQAKSFLVTEALRGEGAHLKLPNGERFMQRFDSRAELAPRDIVARAIDHEMKRLGVDCVYLDISHKPEAFI
KSHFPTVYERCLGFGIDITKQPIPVVPAAHYTCGGVMVDQQGRTDVPGLYAIGETSFTGLHGANRMASNSLLECFVYARS
AAADILEQLPDVLIPRALPAWDASQVTDSDEDVIIAHNWDELRRFMWDYVGIVRTNKRLQRAQHRVRLLLDEIDEFYSNY
KVSRDLIELRNLAQVAELMIRSAMERKESRGLHYTLDYPDLLPEALDTILVPPTYVG

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=510, Percent_Identity=31.7647058823529, Blast_Score=218, Evalue=2e-56,
Organism=Escherichia coli, GI1788928, Length=527, Percent_Identity=64.1366223908918, Blast_Score=701, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=529, Percent_Identity=33.648393194707, Blast_Score=238, Evalue=1e-63,
Organism=Escherichia coli, GI1786942, Length=524, Percent_Identity=31.6793893129771, Blast_Score=207, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17505833, Length=549, Percent_Identity=32.2404371584699, Blast_Score=224, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17550100, Length=538, Percent_Identity=31.5985130111524, Blast_Score=221, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI71986328, Length=428, Percent_Identity=26.8691588785047, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322701, Length=527, Percent_Identity=32.0683111954459, Blast_Score=224, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6322416, Length=534, Percent_Identity=32.2097378277154, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6320788, Length=485, Percent_Identity=26.3917525773196, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322511, Length=483, Percent_Identity=24.0165631469979, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137288, Length=532, Percent_Identity=32.3308270676692, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24655642, Length=532, Percent_Identity=32.3308270676692, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24655647, Length=532, Percent_Identity=32.3308270676692, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24663005, Length=537, Percent_Identity=30.3538175046555, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59507; Mature: 59376

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQFQHDVLVIGSGAAGLSLALTLPGHLRIAVLSKGDLANGSTYWAQGGVAAVLDDTDT
CCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEECCCCCCCCEEEECCCEEEEECCCHH
VESHVDDTLNAGGGLCHEDAVRFTVEHSKEAIQWLIDQGVPFTRDEQSGTEDGGFEFHLT
HHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEE
REGGHSHRRIIHAADATGAAIFTTLLAQAKERPNIELLEQRVAVDLITERRLGLEGERCL
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
GAYVLNRKTGEVDTFGARFVILASGGAAKVYLYTSNPDGACGDGIAMAWRSGCRVANLEF
HHHEECCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHCCCEEEEEEC
NQFHPTCLYHPQAKSFLVTEALRGEGAHLKLPNGERFMQRFDSRAELAPRDIVARAIDHE
CCCCCEEEECCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHCCHHCCCHHHHHHHHHHHH
MKRLGVDCVYLDISHKPEAFIKSHFPTVYERCLGFGIDITKQPIPVVPAAHYTCGGVMVD
HHHCCCEEEEEEECCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCEEEC
QQGRTDVPGLYAIGETSFTGLHGANRMASNSLLECFVYARSAAADILEQLPDVLIPRALP
CCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AWDASQVTDSDEDVIIAHNWDELRRFMWDYVGIVRTNKRLQRAQHRVRLLLDEIDEFYSN
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YKVSRDLIELRNLAQVAELMIRSAMERKESRGLHYTLDYPDLLPEALDTILVPPTYVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
SQQFQHDVLVIGSGAAGLSLALTLPGHLRIAVLSKGDLANGSTYWAQGGVAAVLDDTDT
CCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEECCCCCCCCEEEECCCEEEEECCCHH
VESHVDDTLNAGGGLCHEDAVRFTVEHSKEAIQWLIDQGVPFTRDEQSGTEDGGFEFHLT
HHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEE
REGGHSHRRIIHAADATGAAIFTTLLAQAKERPNIELLEQRVAVDLITERRLGLEGERCL
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
GAYVLNRKTGEVDTFGARFVILASGGAAKVYLYTSNPDGACGDGIAMAWRSGCRVANLEF
HHHEECCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHCCCEEEEEEC
NQFHPTCLYHPQAKSFLVTEALRGEGAHLKLPNGERFMQRFDSRAELAPRDIVARAIDHE
CCCCCEEEECCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHCCHHCCCHHHHHHHHHHHH
MKRLGVDCVYLDISHKPEAFIKSHFPTVYERCLGFGIDITKQPIPVVPAAHYTCGGVMVD
HHHCCCEEEEEEECCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCEEEC
QQGRTDVPGLYAIGETSFTGLHGANRMASNSLLECFVYARSAAADILEQLPDVLIPRALP
CCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AWDASQVTDSDEDVIIAHNWDELRRFMWDYVGIVRTNKRLQRAQHRVRLLLDEIDEFYSN
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YKVSRDLIELRNLAQVAELMIRSAMERKESRGLHYTLDYPDLLPEALDTILVPPTYVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]