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The map label for this gene is paaF [C]

Identifier: 77457575

GI number: 77457575

Start: 1522883

End: 1523995

Strand: Reverse

Name: paaF [C]

Synonym: Pfl01_1348

Alternate gene names: 77457575

Gene position: 1523995-1522883 (Counterclockwise)

Preceding gene: 77457577

Following gene: 77457570

Centisome position: 23.67

GC content: 61.81

Gene sequence:

>1113_bases
ATGAATCTGCACTTCGAAGAACTCACCGGCACCGACGGCGCGCGCATCGGCGTGGCAACGCTGGATGCCGAAAAGTCGCT
CAATGCGCTGTCCCTGCCGATGATCCACGCCCTGAGTGACAAACTGAGCGTTTGGGCCAAGGATCCACAAATCGTCTGCG
TGCTGCTGCGCGGCAATGGCGCCAAGGCCTTCTGCGCCGGCGGCGAAGTGCGCAGCCTGGTGGAAGCCTGTCGCGCCCAT
CCCGGCGAAGTACCGCCGCTGGCCGCACAGTTTTTCAACGCTGAATACCGTCTGGACTACAGCCTGCACACTTACCCGAA
ACCGTTGATCTGCTGGGGCCATGGTTATGTGCTCGGCGGCGGCATGGGCCTGCTGCAAGGTGCGAGCATCCGCATCGTCA
CGCCGAGCAGTCGTCTGGCGATGCCGGAAATCACCATCGGCCTGTACCCGGATGTCGGTGCCAGTTGGTTCCTGTCACGA
CTGCCGGGCAAGCTCGGCTTGTTCCTTGGTTTGACCGGCGCGCACATGAACGGCCGCGATGCGATCGATCTGGACCTGGC
CGACCGTTTCCTGCTCGACGAACAGCAACAGGAACTGATCGACGGCTTGTTGCAGTTGAACTGGCAGGAACAGACTGCCA
TGCAGCTCAACAGCCTGCTCAAGGCGCTGCAGCAGGAAGCCGTGGCGCAGATGCCTGAAGCGCAATGGCTGCCACGGCGC
CAGGTAATCGATGATCTGTTGGATGTCAGCGATGTAACGTGCGCCTGGAAAGCCATCAGCCTGCATCGCGACAGCAACGA
CCTGCTGATTGCCCGTGCGGCGAAAACCATGAGTGAAGGCTCGCCGCTTACCGCGCATCTGGTCTGGGAACAGCTCATCC
GCGCGCGCCACTTGTCACTGGCCGAAGTCTTCCAGATGGAATACACCCTGAGCCTCAATTGCTGCCGGCATCCGGAATTC
AGCGAAGGGGTCCGGGCGCGGCTGATCGACAAGGATCACAAACCGCACTGGCACTGGCCGGACATCGCCCGGGTGCCGGA
CGCGGTGGTCGAGGCGCACTTTCACAAAGTCTGGGAGGGGCGGCATCCGTTGGCGGATCTCTCCAGTTACTGA

Upstream 100 bases:

>100_bases
CTAGGAAAGCCGACCACGCTTGTCAATTGATCTGACGCAGATCCGGCACTTTCCCACACAGCGATCATACTGATCGTTCA
AATTCCCGATCGAGGTCACG

Downstream 100 bases:

>100_bases
AATCCATGTCTAGTCCTGAAATAGGTTTACACCTGTTTCAGCCTCAAAACGCCCGATGCACCGCATCGGGTGTTTTGTAT
TTCAGCGACAGATGAGGCCG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MNLHFEELTGTDGARIGVATLDAEKSLNALSLPMIHALSDKLSVWAKDPQIVCVLLRGNGAKAFCAGGEVRSLVEACRAH
PGEVPPLAAQFFNAEYRLDYSLHTYPKPLICWGHGYVLGGGMGLLQGASIRIVTPSSRLAMPEITIGLYPDVGASWFLSR
LPGKLGLFLGLTGAHMNGRDAIDLDLADRFLLDEQQQELIDGLLQLNWQEQTAMQLNSLLKALQQEAVAQMPEAQWLPRR
QVIDDLLDVSDVTCAWKAISLHRDSNDLLIARAAKTMSEGSPLTAHLVWEQLIRARHLSLAEVFQMEYTLSLNCCRHPEF
SEGVRARLIDKDHKPHWHWPDIARVPDAVVEAHFHKVWEGRHPLADLSSY

Sequences:

>Translated_370_residues
MNLHFEELTGTDGARIGVATLDAEKSLNALSLPMIHALSDKLSVWAKDPQIVCVLLRGNGAKAFCAGGEVRSLVEACRAH
PGEVPPLAAQFFNAEYRLDYSLHTYPKPLICWGHGYVLGGGMGLLQGASIRIVTPSSRLAMPEITIGLYPDVGASWFLSR
LPGKLGLFLGLTGAHMNGRDAIDLDLADRFLLDEQQQELIDGLLQLNWQEQTAMQLNSLLKALQQEAVAQMPEAQWLPRR
QVIDDLLDVSDVTCAWKAISLHRDSNDLLIARAAKTMSEGSPLTAHLVWEQLIRARHLSLAEVFQMEYTLSLNCCRHPEF
SEGVRARLIDKDHKPHWHWPDIARVPDAVVEAHFHKVWEGRHPLADLSSY
>Mature_370_residues
MNLHFEELTGTDGARIGVATLDAEKSLNALSLPMIHALSDKLSVWAKDPQIVCVLLRGNGAKAFCAGGEVRSLVEACRAH
PGEVPPLAAQFFNAEYRLDYSLHTYPKPLICWGHGYVLGGGMGLLQGASIRIVTPSSRLAMPEITIGLYPDVGASWFLSR
LPGKLGLFLGLTGAHMNGRDAIDLDLADRFLLDEQQQELIDGLLQLNWQEQTAMQLNSLLKALQQEAVAQMPEAQWLPRR
QVIDDLLDVSDVTCAWKAISLHRDSNDLLIARAAKTMSEGSPLTAHLVWEQLIRARHLSLAEVFQMEYTLSLNCCRHPEF
SEGVRARLIDKDHKPHWHWPDIARVPDAVVEAHFHKVWEGRHPLADLSSY

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=339, Percent_Identity=35.3982300884956, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI37594469, Length=299, Percent_Identity=34.1137123745819, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI194097323, Length=178, Percent_Identity=30.3370786516854, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=178, Percent_Identity=28.6516853932584, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI221142681, Length=191, Percent_Identity=27.2251308900524, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790281, Length=212, Percent_Identity=26.4150943396226, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI25144157, Length=350, Percent_Identity=34.5714285714286, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI25144160, Length=350, Percent_Identity=34.5714285714286, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6320241, Length=353, Percent_Identity=27.7620396600567, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28571729, Length=347, Percent_Identity=31.9884726224784, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI28571730, Length=347, Percent_Identity=31.9884726224784, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI20129971, Length=186, Percent_Identity=30.1075268817204, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24653477, Length=186, Percent_Identity=30.1075268817204, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 41135; Mature: 41135

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHFEELTGTDGARIGVATLDAEKSLNALSLPMIHALSDKLSVWAKDPQIVCVLLRGNG
CCCCCHHHCCCCCCEEEEEEECHHHCCCHHHHHHHHHHHHHHHEEECCCEEEEEEEECCC
AKAFCAGGEVRSLVEACRAHPGEVPPLAAQFFNAEYRLDYSLHTYPKPLICWGHGYVLGG
CEEEECCHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEEEECCCCCCEEEECCCEEEEC
GMGLLQGASIRIVTPSSRLAMPEITIGLYPDVGASWFLSRLPGKLGLFLGLTGAHMNGRD
CHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHCCCHHEEEEEEECCCCCCCC
AIDLDLADRFLLDEQQQELIDGLLQLNWQEQTAMQLNSLLKALQQEAVAQMPEAQWLPRR
EEEHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
QVIDDLLDVSDVTCAWKAISLHRDSNDLLIARAAKTMSEGSPLTAHLVWEQLIRARHLSL
HHHHHHHCCCCHHHHEEEEEEECCCCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHCCH
AEVFQMEYTLSLNCCRHPEFSEGVRARLIDKDHKPHWHWPDIARVPDAVVEAHFHKVWEG
HHHHHHHEEEEEECCCCCCHHCCHHHHHCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCC
RHPLADLSSY
CCCCHHCCCC
>Mature Secondary Structure
MNLHFEELTGTDGARIGVATLDAEKSLNALSLPMIHALSDKLSVWAKDPQIVCVLLRGNG
CCCCCHHHCCCCCCEEEEEEECHHHCCCHHHHHHHHHHHHHHHEEECCCEEEEEEEECCC
AKAFCAGGEVRSLVEACRAHPGEVPPLAAQFFNAEYRLDYSLHTYPKPLICWGHGYVLGG
CEEEECCHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEEEECCCCCCEEEECCCEEEEC
GMGLLQGASIRIVTPSSRLAMPEITIGLYPDVGASWFLSRLPGKLGLFLGLTGAHMNGRD
CHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHCCCHHEEEEEEECCCCCCCC
AIDLDLADRFLLDEQQQELIDGLLQLNWQEQTAMQLNSLLKALQQEAVAQMPEAQWLPRR
EEEHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
QVIDDLLDVSDVTCAWKAISLHRDSNDLLIARAAKTMSEGSPLTAHLVWEQLIRARHLSL
HHHHHHHCCCCHHHHEEEEEEECCCCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHCCH
AEVFQMEYTLSLNCCRHPEFSEGVRARLIDKDHKPHWHWPDIARVPDAVVEAHFHKVWEG
HHHHHHHEEEEEECCCCCCHHCCHHHHHCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCC
RHPLADLSSY
CCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA