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The map label for this gene is proS [H]

Identifier: 77457424

GI number: 77457424

Start: 1368425

End: 1370140

Strand: Reverse

Name: proS [H]

Synonym: Pfl01_1197

Alternate gene names: 77457424

Gene position: 1370140-1368425 (Counterclockwise)

Preceding gene: 77457425

Following gene: 77457423

Centisome position: 21.28

GC content: 61.77

Gene sequence:

>1716_bases
ATGCGCACCAGTCAATTTTTGCTCGCCACACAGAAAGAAACGCCTTCCGACGCGGTCGTGATCAGCCACCAGCTGATGCT
GCGCGCCGGCATGATCCGCAAACTCGCCTCGGGCCTGTACACCTGGCTGCCGATGGGCTTGCGAGTAATGCGCAAGGTGG
AAGCGATCGTTCGCGAAGAAATGGACGCCGCCGGCTCTCTGGAAGTGTTGATGCCGAGCACCCAACCGGCCGAGCTGTGG
CAGGAATCGGGACGCTGGGAAGAATACGGCCCTGAGCTGCTGCGCATCAAAGACCGCCACGGTCGTGATTTCTGCGCGGG
CCCGACCCACGAAGAAGTGATCACCGATCTGATGCGCAACGAGTTGAGCAGCTACAAACAGCTGCCGATCAACCTGTACC
AGATCCAGACCAAATTCCGTGACGAAATCCGTCCACGCTTCGGCCTGATGCGCGGTCGCGAATTCATCATGAAGGACTCG
TACTCCTTCCATGCGGATCAGGCGTCGCTGCAGGTCACCTATGACCGCATGCACGATGCGTACTGCAACATCTTCACCCG
CCTGGGCCTGAAGTTCCGTCCGGTGGAAGCCGACAACGGTTCGATCGGTGGCGCCGGTTCCCACGAATTCCACGTGCTGG
CCGAGTCCGGTGAAGACGATATCGTCTTCAGCAACGGCTCCGACTATGCGGCGAACATCGAGAAAGCCGAAGCCGTGCCG
CGTGAAACCTCGCGCGCCGCGCCAAGCGAAGAGCTGCGTCTGGTCGACACGCCAGACACCAAGACCATCGCGGCCCTGGT
GGAGAAATTCAATCTGCCGATTGAAAAGACCATCAAGACCCTGATCGTGCACGCCGAAGAAGAAGGCAAGCTGATCGCCC
TGATCATCCGTGGCGACCACGAGCTGAACGAAATCAAGGCTGCCAATCAGCCAGGCGTTGCCAGCCCGCTGGTCATGGCC
TCCGATGCCGAACTGCGTGACGCCATTGGCGCCGGCGCCGGTTCCCTCGGCCCGCTGAACCTGCCGCTGCCGATCATCAT
CGACCGTTCGGTCGAGCTGATGAGCGACTTCGCCATCGGTGCCAACATCGACGACAAGCACTACTTCGGCGTGAACTGGG
AGCGTGATCTGCCGGTTCCGACCGTGGCCGACCTGCGCAACGTCGTGGCCGGCGACCCGAGCCCGGACGGCAAGGGCACC
CTGGAAATCAAGCGCGGCATCGAAGTCGGACACATCTTCCAGCTGGGCAACAAGTACAGCAAGGCGATGAAGTGCGAAGT
GCTGGGCGAGAACGGCAAGCCGGTGACCCTGGAAATGGGTTGCTACGGTATCGGCGTGTCCCGCGTGGTGGCTGCCGCCA
TCGAGCAGAACAACGACGAGAACGGCATCATCTGGAGCGACGCCCTGGCACCGTTCCAGATCGCTCTGGTGCCACTGCGC
TATGAAACCGAGCTGGTGCGTGAAGCCACCGACAAGCTGTACGCGGAACTGACCGCGGCCGGCTTCGAAGTGCTGCTGGA
CGACCGCGACAAGAAAACCAGCCCGGGCATCAAGTTCGCGGACATGGAGCTGATCGGCATTCCGCACCGGATCGTGGTCA
GCGACCGCGGCCTCGCCGAAGGCAACCTGGAATACAAGAGCCGTACCGAAGGCCAGGCGCAAGCGCTGCCGGTTGCCGAC
GTGCTGTCCTTCCTTCAGGCCCGTATCCGCCGCTGA

Upstream 100 bases:

>100_bases
GCGAGAGGGTTCGCGCCGGGGTTGCACTTCTGTACGTCGGCGGATCTCGCCGTTACACTGCTCCGTCATTTCCAGTCATA
GCAACCGACAACGGCCAACC

Downstream 100 bases:

>100_bases
AACCAGATAGAGACGTCATGTTCAAGCGAAACACCTTAGGCCTCGGTGGTGCCGCCCTGTGCGGCACCCTGCTGGTCAGC
GGCTGTGCCAATCACATGTC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRTSQFLLATQKETPSDAVVISHQLMLRAGMIRKLASGLYTWLPMGLRVMRKVEAIVREEMDAAGSLEVLMPSTQPAELW
QESGRWEEYGPELLRIKDRHGRDFCAGPTHEEVITDLMRNELSSYKQLPINLYQIQTKFRDEIRPRFGLMRGREFIMKDS
YSFHADQASLQVTYDRMHDAYCNIFTRLGLKFRPVEADNGSIGGAGSHEFHVLAESGEDDIVFSNGSDYAANIEKAEAVP
RETSRAAPSEELRLVDTPDTKTIAALVEKFNLPIEKTIKTLIVHAEEEGKLIALIIRGDHELNEIKAANQPGVASPLVMA
SDAELRDAIGAGAGSLGPLNLPLPIIIDRSVELMSDFAIGANIDDKHYFGVNWERDLPVPTVADLRNVVAGDPSPDGKGT
LEIKRGIEVGHIFQLGNKYSKAMKCEVLGENGKPVTLEMGCYGIGVSRVVAAAIEQNNDENGIIWSDALAPFQIALVPLR
YETELVREATDKLYAELTAAGFEVLLDDRDKKTSPGIKFADMELIGIPHRIVVSDRGLAEGNLEYKSRTEGQAQALPVAD
VLSFLQARIRR

Sequences:

>Translated_571_residues
MRTSQFLLATQKETPSDAVVISHQLMLRAGMIRKLASGLYTWLPMGLRVMRKVEAIVREEMDAAGSLEVLMPSTQPAELW
QESGRWEEYGPELLRIKDRHGRDFCAGPTHEEVITDLMRNELSSYKQLPINLYQIQTKFRDEIRPRFGLMRGREFIMKDS
YSFHADQASLQVTYDRMHDAYCNIFTRLGLKFRPVEADNGSIGGAGSHEFHVLAESGEDDIVFSNGSDYAANIEKAEAVP
RETSRAAPSEELRLVDTPDTKTIAALVEKFNLPIEKTIKTLIVHAEEEGKLIALIIRGDHELNEIKAANQPGVASPLVMA
SDAELRDAIGAGAGSLGPLNLPLPIIIDRSVELMSDFAIGANIDDKHYFGVNWERDLPVPTVADLRNVVAGDPSPDGKGT
LEIKRGIEVGHIFQLGNKYSKAMKCEVLGENGKPVTLEMGCYGIGVSRVVAAAIEQNNDENGIIWSDALAPFQIALVPLR
YETELVREATDKLYAELTAAGFEVLLDDRDKKTSPGIKFADMELIGIPHRIVVSDRGLAEGNLEYKSRTEGQAQALPVAD
VLSFLQARIRR
>Mature_571_residues
MRTSQFLLATQKETPSDAVVISHQLMLRAGMIRKLASGLYTWLPMGLRVMRKVEAIVREEMDAAGSLEVLMPSTQPAELW
QESGRWEEYGPELLRIKDRHGRDFCAGPTHEEVITDLMRNELSSYKQLPINLYQIQTKFRDEIRPRFGLMRGREFIMKDS
YSFHADQASLQVTYDRMHDAYCNIFTRLGLKFRPVEADNGSIGGAGSHEFHVLAESGEDDIVFSNGSDYAANIEKAEAVP
RETSRAAPSEELRLVDTPDTKTIAALVEKFNLPIEKTIKTLIVHAEEEGKLIALIIRGDHELNEIKAANQPGVASPLVMA
SDAELRDAIGAGAGSLGPLNLPLPIIIDRSVELMSDFAIGANIDDKHYFGVNWERDLPVPTVADLRNVVAGDPSPDGKGT
LEIKRGIEVGHIFQLGNKYSKAMKCEVLGENGKPVTLEMGCYGIGVSRVVAAAIEQNNDENGIIWSDALAPFQIALVPLR
YETELVREATDKLYAELTAAGFEVLLDDRDKKTSPGIKFADMELIGIPHRIVVSDRGLAEGNLEYKSRTEGQAQALPVAD
VLSFLQARIRR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=219, Percent_Identity=49.3150684931507, Blast_Score=229, Evalue=6e-60,
Organism=Escherichia coli, GI1786392, Length=573, Percent_Identity=66.6666666666667, Blast_Score=802, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=264, Percent_Identity=38.6363636363636, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=36.4705882352941, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=535, Percent_Identity=31.4018691588785, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24656200, Length=273, Percent_Identity=33.6996336996337, Blast_Score=169, Evalue=5e-42,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63208; Mature: 63208

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQFLLATQKETPSDAVVISHQLMLRAGMIRKLASGLYTWLPMGLRVMRKVEAIVREE
CCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDAAGSLEVLMPSTQPAELWQESGRWEEYGPELLRIKDRHGRDFCAGPTHEEVITDLMRN
HCCCCCEEEEECCCCHHHHHHHCCCCHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
ELSSYKQLPINLYQIQTKFRDEIRPRFGLMRGREFIMKDSYSFHADQASLQVTYDRMHDA
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCEEEEEHHHHHHH
YCNIFTRLGLKFRPVEADNGSIGGAGSHEFHVLAESGEDDIVFSNGSDYAANIEKAEAVP
HHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCHHHHCCC
RETSRAAPSEELRLVDTPDTKTIAALVEKFNLPIEKTIKTLIVHAEEEGKLIALIIRGDH
CHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCEEEEEEECCC
ELNEIKAANQPGVASPLVMASDAELRDAIGAGAGSLGPLNLPLPIIIDRSVELMSDFAIG
CHHHHHCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHCCC
ANIDDKHYFGVNWERDLPVPTVADLRNVVAGDPSPDGKGTLEIKRGIEVGHIFQLGNKYS
CCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHHHHHHH
KAMKCEVLGENGKPVTLEMGCYGIGVSRVVAAAIEQNNDENGIIWSDALAPFQIALVPLR
HEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEC
YETELVREATDKLYAELTAAGFEVLLDDRDKKTSPGIKFADMELIGIPHRIVVSDRGLAE
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEECEEEECCCEEEEEECCCCCC
GNLEYKSRTEGQAQALPVADVLSFLQARIRR
CCCCCCCCCCCCHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTSQFLLATQKETPSDAVVISHQLMLRAGMIRKLASGLYTWLPMGLRVMRKVEAIVREE
CCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDAAGSLEVLMPSTQPAELWQESGRWEEYGPELLRIKDRHGRDFCAGPTHEEVITDLMRN
HCCCCCEEEEECCCCHHHHHHHCCCCHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
ELSSYKQLPINLYQIQTKFRDEIRPRFGLMRGREFIMKDSYSFHADQASLQVTYDRMHDA
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCEEEEEHHHHHHH
YCNIFTRLGLKFRPVEADNGSIGGAGSHEFHVLAESGEDDIVFSNGSDYAANIEKAEAVP
HHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCHHHHCCC
RETSRAAPSEELRLVDTPDTKTIAALVEKFNLPIEKTIKTLIVHAEEEGKLIALIIRGDH
CHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCEEEEEEECCC
ELNEIKAANQPGVASPLVMASDAELRDAIGAGAGSLGPLNLPLPIIIDRSVELMSDFAIG
CHHHHHCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHCCC
ANIDDKHYFGVNWERDLPVPTVADLRNVVAGDPSPDGKGTLEIKRGIEVGHIFQLGNKYS
CCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHHHHHHH
KAMKCEVLGENGKPVTLEMGCYGIGVSRVVAAAIEQNNDENGIIWSDALAPFQIALVPLR
HEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEC
YETELVREATDKLYAELTAAGFEVLLDDRDKKTSPGIKFADMELIGIPHRIVVSDRGLAE
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEECEEEECCCEEEEEECCCCCC
GNLEYKSRTEGQAQALPVADVLSFLQARIRR
CCCCCCCCCCCCHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA