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The map label for this gene is dapE [H]

Identifier: 77457315

GI number: 77457315

Start: 1258204

End: 1259355

Strand: Reverse

Name: dapE [H]

Synonym: Pfl01_1088

Alternate gene names: 77457315

Gene position: 1259355-1258204 (Counterclockwise)

Preceding gene: 77457316

Following gene: 77457314

Centisome position: 19.56

GC content: 64.41

Gene sequence:

>1152_bases
ATGACGGCCCACGCCGACCTTTCGCCGACCCTCCAACTCGCCATCGACCTGATCCGCCGTCCGTCCGTGACGCCGGTCGA
CGCCGATTGCCAGAAGCAGATGATGCAGCGCCTGGGCGATGCCGGTTTCCAGCTGGAACCGATGCGCATCGAAGATGTGG
ATAACTTCTGGGCGACTCACGGCAAAGGCGACGGCCCGGTGCTGTGCTTCGCCGGCCACACCGACGTGGTGCCGACCGGC
CCGGTGACTGCCTGGCAGATCGACCCGTTCAACGCGGTGATCGACGAGCACGGCATGCTCTGCGGCCGTGGGGCGGCGGA
CATGAAAGGCAGCCTGGCCTCGATGACTGTGGCAGCCGAGCGTTTTGTCGCCGACTACCCGGATCACAAGGGCAAGGTCG
CGTTCCTGATCACCAGCGACGAAGAAGGCCCGGCGCATCACGGCACCAAGGCCGTGGTCGAGCGTCTGGCCGCCCGTAAC
GAGCGTCTGGACTGGTGCATCGTCGGCGAACCGTCGAGCACCACTTTGGTGGGCGATGTGGTGAAGAACGGTCGTCGCGG
CTCCCTCGGCGCCAAACTGACCGTGCGCGGCGTGCAGGGCCACGTGGCCTATCCGCACCTGGCGAAGAACCCGATCCACC
TCGCCGCCCCGGCTCTGGCCGAACTGGCCGCCGAGCATTGGGATCATGGCAACGATTTCTTCCCGCCGACCAGTTTCCAG
ATTTCCAACGTCAATTCCGGCACCGGCGCGACCAACGTGATTCCGGGTGATCTGGTGGCGGTGTTCAACTTCCGTTTCTC
CACCGAATCCACCGTGGAAGGCCTGCAAAAGCGCGTCGCCGATATTCTCGACAAACATGGCCTGGACTGGCACATCGACT
GGGCGCTGTCCGGTCTGCCGTTCCTCACCGAGCCTGGCGCACTGCTCGACGCGGTGTCTTCGAGCATCAAGGACATCACC
GGCCGCGAGACCAAGGCATCCACCAGCGGCGGCACCTCCGACGGTCGTTTCATCGCGACCATGGGCACGCAGGTTGTTGA
ACTGGGCCCGGTCAACGCGACCATCCACCAGGTCAACGAGCGCGTACTGGCGGCCGATCTCGACGTGCTGACCGAAATCT
ACTACCAGACCCTGATCAAGTTGCTCGCCTGA

Upstream 100 bases:

>100_bases
AATCCTCACCGATGCACTAAACGGCGGCCAATGGTCGCCGTTTTGCTTTAAGATAACCGCCATTTTGCGCGCCTTGCCCC
CCCAGCTTTCGGAGTTTTCC

Downstream 100 bases:

>100_bases
TGCTCGCGTGCCCGATCTGCAGTGAACCGCTGAACGCGGTGGAAAACGGCGTGATCTGCCCTGCCGGCCACCGCTTTGAC
CGCGCGCGCCAGGGTTATCT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTAHADLSPTLQLAIDLIRRPSVTPVDADCQKQMMQRLGDAGFQLEPMRIEDVDNFWATHGKGDGPVLCFAGHTDVVPTG
PVTAWQIDPFNAVIDEHGMLCGRGAADMKGSLASMTVAAERFVADYPDHKGKVAFLITSDEEGPAHHGTKAVVERLAARN
ERLDWCIVGEPSSTTLVGDVVKNGRRGSLGAKLTVRGVQGHVAYPHLAKNPIHLAAPALAELAAEHWDHGNDFFPPTSFQ
ISNVNSGTGATNVIPGDLVAVFNFRFSTESTVEGLQKRVADILDKHGLDWHIDWALSGLPFLTEPGALLDAVSSSIKDIT
GRETKASTSGGTSDGRFIATMGTQVVELGPVNATIHQVNERVLAADLDVLTEIYYQTLIKLLA

Sequences:

>Translated_383_residues
MTAHADLSPTLQLAIDLIRRPSVTPVDADCQKQMMQRLGDAGFQLEPMRIEDVDNFWATHGKGDGPVLCFAGHTDVVPTG
PVTAWQIDPFNAVIDEHGMLCGRGAADMKGSLASMTVAAERFVADYPDHKGKVAFLITSDEEGPAHHGTKAVVERLAARN
ERLDWCIVGEPSSTTLVGDVVKNGRRGSLGAKLTVRGVQGHVAYPHLAKNPIHLAAPALAELAAEHWDHGNDFFPPTSFQ
ISNVNSGTGATNVIPGDLVAVFNFRFSTESTVEGLQKRVADILDKHGLDWHIDWALSGLPFLTEPGALLDAVSSSIKDIT
GRETKASTSGGTSDGRFIATMGTQVVELGPVNATIHQVNERVLAADLDVLTEIYYQTLIKLLA
>Mature_382_residues
TAHADLSPTLQLAIDLIRRPSVTPVDADCQKQMMQRLGDAGFQLEPMRIEDVDNFWATHGKGDGPVLCFAGHTDVVPTGP
VTAWQIDPFNAVIDEHGMLCGRGAADMKGSLASMTVAAERFVADYPDHKGKVAFLITSDEEGPAHHGTKAVVERLAARNE
RLDWCIVGEPSSTTLVGDVVKNGRRGSLGAKLTVRGVQGHVAYPHLAKNPIHLAAPALAELAAEHWDHGNDFFPPTSFQI
SNVNSGTGATNVIPGDLVAVFNFRFSTESTVEGLQKRVADILDKHGLDWHIDWALSGLPFLTEPGALLDAVSSSIKDITG
RETKASTSGGTSDGRFIATMGTQVVELGPVNATIHQVNERVLAADLDVLTEIYYQTLIKLLA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=59.4666666666667, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1790395, Length=227, Percent_Identity=30.3964757709251, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24649210, Length=334, Percent_Identity=25.4491017964072, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41081; Mature: 40950

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHADLSPTLQLAIDLIRRPSVTPVDADCQKQMMQRLGDAGFQLEPMRIEDVDNFWATH
CCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEECCEECCCCHHHHHCC
GKGDGPVLCFAGHTDVVPTGPVTAWQIDPFNAVIDEHGMLCGRGAADMKGSLASMTVAAE
CCCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHCCCEECCCCCCCCCCHHHHHHHHH
RFVADYPDHKGKVAFLITSDEEGPAHHGTKAVVERLAARNERLDWCIVGEPSSTTLVGDV
HHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEHHH
VKNGRRGSLGAKLTVRGVQGHVAYPHLAKNPIHLAAPALAELAAEHWDHGNDFFPPTSFQ
HHCCCCCCCCCEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEE
ISNVNSGTGATNVIPGDLVAVFNFRFSTESTVEGLQKRVADILDKHGLDWHIDWALSGLP
EECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
FLTEPGALLDAVSSSIKDITGRETKASTSGGTSDGRFIATMGTQVVELGPVNATIHQVNE
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHH
RVLAADLDVLTEIYYQTLIKLLA
HHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TAHADLSPTLQLAIDLIRRPSVTPVDADCQKQMMQRLGDAGFQLEPMRIEDVDNFWATH
CCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEECCEECCCCHHHHHCC
GKGDGPVLCFAGHTDVVPTGPVTAWQIDPFNAVIDEHGMLCGRGAADMKGSLASMTVAAE
CCCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHCCCEECCCCCCCCCCHHHHHHHHH
RFVADYPDHKGKVAFLITSDEEGPAHHGTKAVVERLAARNERLDWCIVGEPSSTTLVGDV
HHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEHHH
VKNGRRGSLGAKLTVRGVQGHVAYPHLAKNPIHLAAPALAELAAEHWDHGNDFFPPTSFQ
HHCCCCCCCCCEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEE
ISNVNSGTGATNVIPGDLVAVFNFRFSTESTVEGLQKRVADILDKHGLDWHIDWALSGLP
EECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
FLTEPGALLDAVSSSIKDITGRETKASTSGGTSDGRFIATMGTQVVELGPVNATIHQVNE
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHH
RVLAADLDVLTEIYYQTLIKLLA
HHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA