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The map label for this gene is rlmE

Identifier: 77456998

GI number: 77456998

Start: 905142

End: 905771

Strand: Direct

Name: rlmE

Synonym: Pfl01_0771

Alternate gene names: 77456998

Gene position: 905142-905771 (Clockwise)

Preceding gene: 77456996

Following gene: 77456999

Centisome position: 14.06

GC content: 56.03

Gene sequence:

>630_bases
ATGGCGCGTTCCAAGACAAGCCTTGGTTGGCTGAAAAGACATGTCAATGATCCCTATGTGAAGCAAGCGCAGAAGGATGG
CTACCGCTCGCGTGCGAGTTACAAGCTTCTGGAGATCCAGGAGAAATACAAACTGATCCGTCCGGGCATGAACGTCGTCG
ACCTGGGCGCGGCGCCTGGCGGCTGGTCGCAGGTCACCAGCCGGCTGATCGGTGGTCAGGGGCGACTGATCGCCTCGGAC
ATCCTGGAAATGGACAGCATCCCGGACGTGACTTTCATCCAGGGTGACTTCACCGAGGATGCAGTGCTCGCTCAGATCCT
TGAGGCTGTGGGTAATTCGCAGGTGGACCTTGTGATTTCCGATATGGCCCCCAATATGAGTGGTACGCCTGAAGTGGACA
TGCCAAAAGCCATGTTCCTTTGCGAGCTGGCGCTTGATCTGGCGGAACGGATACTCAAGCCGGGTGGCAATTTCGTGATC
AAGATTTTTCAGGGCGAAGGGTTCGATACTTACCTGAAGGATGCTCGTAAGAAGTTCGACAAGATCCAGATGATCAAGCC
GGACTCCTCTCGTGGCAGCTCTCGCGAGCAGTACATGCTGGCTTGGGGCTACCGCGGTCGTAGCGAGTAA

Upstream 100 bases:

>100_bases
ATCTGACCCTTTCGTCTGGATTCTGTAAAATGGCGGCCATTTTACCCGAGGGCTCGTGGATCCGCCCAATTAATCACGAC
CCTAATCAACGAGGTGCCCA

Downstream 100 bases:

>100_bases
AACGAGGTTTTTTGGCGGGGCGATAGGTTTTTCATATTTCGCCCCGCGCGCATAAGCGAATATTGTGTAGAAAGTGTTTC
ACAAAGGGTTACAGACGGCG

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MARSKTSLGWLKRHVNDPYVKQAQKDGYRSRASYKLLEIQEKYKLIRPGMNVVDLGAAPGGWSQVTSRLIGGQGRLIASD
ILEMDSIPDVTFIQGDFTEDAVLAQILEAVGNSQVDLVISDMAPNMSGTPEVDMPKAMFLCELALDLAERILKPGGNFVI
KIFQGEGFDTYLKDARKKFDKIQMIKPDSSRGSSREQYMLAWGYRGRSE

Sequences:

>Translated_209_residues
MARSKTSLGWLKRHVNDPYVKQAQKDGYRSRASYKLLEIQEKYKLIRPGMNVVDLGAAPGGWSQVTSRLIGGQGRLIASD
ILEMDSIPDVTFIQGDFTEDAVLAQILEAVGNSQVDLVISDMAPNMSGTPEVDMPKAMFLCELALDLAERILKPGGNFVI
KIFQGEGFDTYLKDARKKFDKIQMIKPDSSRGSSREQYMLAWGYRGRSE
>Mature_208_residues
ARSKTSLGWLKRHVNDPYVKQAQKDGYRSRASYKLLEIQEKYKLIRPGMNVVDLGAAPGGWSQVTSRLIGGQGRLIASDI
LEMDSIPDVTFIQGDFTEDAVLAQILEAVGNSQVDLVISDMAPNMSGTPEVDMPKAMFLCELALDLAERILKPGGNFVIK
IFQGEGFDTYLKDARKKFDKIQMIKPDSSRGSSREQYMLAWGYRGRSE

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=219, Percent_Identity=35.6164383561644, Blast_Score=154, Evalue=4e-38,
Organism=Homo sapiens, GI29029591, Length=199, Percent_Identity=37.1859296482412, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI29029589, Length=199, Percent_Identity=37.1859296482412, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI7110661, Length=199, Percent_Identity=37.1859296482412, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=28.7234042553192, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1789569, Length=203, Percent_Identity=58.6206896551724, Blast_Score=249, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI17554650, Length=200, Percent_Identity=32.5, Blast_Score=119, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71987550, Length=201, Percent_Identity=31.8407960199005, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17553474, Length=214, Percent_Identity=29.9065420560748, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71987561, Length=201, Percent_Identity=31.8407960199005, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17553860, Length=201, Percent_Identity=31.8407960199005, Blast_Score=109, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6319535, Length=203, Percent_Identity=38.9162561576355, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=32.4607329842932, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=90, Percent_Identity=38.8888888888889, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356387, Length=216, Percent_Identity=34.2592592592593, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24647580, Length=207, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24648639, Length=202, Percent_Identity=29.2079207920792, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859957, Length=200, Percent_Identity=28.5, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_PSEPF (Q3KI92)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346503.1
- ProteinModelPortal:   Q3KI92
- SMR:   Q3KI92
- STRING:   Q3KI92
- GeneID:   3715229
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_0771
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK868873
- BioCyc:   PFLU205922:PFL_0771-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23313; Mature: 23182

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: ACT_SITE 161-161 BINDING 60-60 BINDING 62-62 BINDING 80-80 BINDING 96-96 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSKTSLGWLKRHVNDPYVKQAQKDGYRSRASYKLLEIQEKYKLIRPGMNVVDLGAAPG
CCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
GWSQVTSRLIGGQGRLIASDILEMDSIPDVTFIQGDFTEDAVLAQILEAVGNSQVDLVIS
CHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEE
DMAPNMSGTPEVDMPKAMFLCELALDLAERILKPGGNFVIKIFQGEGFDTYLKDARKKFD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
KIQMIKPDSSRGSSREQYMLAWGYRGRSE
HEEEECCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
ARSKTSLGWLKRHVNDPYVKQAQKDGYRSRASYKLLEIQEKYKLIRPGMNVVDLGAAPG
CCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
GWSQVTSRLIGGQGRLIASDILEMDSIPDVTFIQGDFTEDAVLAQILEAVGNSQVDLVIS
CHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEE
DMAPNMSGTPEVDMPKAMFLCELALDLAERILKPGGNFVIKIFQGEGFDTYLKDARKKFD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
KIQMIKPDSSRGSSREQYMLAWGYRGRSE
HEEEECCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA