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The map label for this gene is aceF [C]

Identifier: 77456690

GI number: 77456690

Start: 531451

End: 533406

Strand: Reverse

Name: aceF [C]

Synonym: Pfl01_0462

Alternate gene names: 77456690

Gene position: 533406-531451 (Counterclockwise)

Preceding gene: 77456691

Following gene: 77456689

Centisome position: 8.28

GC content: 64.16

Gene sequence:

>1956_bases
GTGAGCGAACTCATTCGCGTACCTGACATCGGCAGCGGTGAAGGTGAAGTAATCGAACTGTTTGTGAAGGTCGGCGACCG
TATCGAAGCCGACCAGAGCATCCTGACTCTGGAATCGGACAAGGCCAGCATGGAAGTGCCGGCCCCGAAGGCCGGCGTCA
TCAAAAGCCTGAAAGTGAAGCTGGGCGATCGCCTGAAGGAAGGCGACGAACTGCTGGAACTGGAAGTCGAGGGCGCCGCG
CAAGCGGCCCCTGCTCCGGCTGCTGCGCCTGCCGCCAAGGCCGAAGCTGCACCGGCCGCCGCTCCTGCACCGGCTGCTCC
GGCCGCTGCCCCTGCTGCCGCTTCGGTTCAGCAAGTGCACGTGCCGGATATCGGTTCGTCGGGCAAGGCCCAGATCATCG
AGATCCAGGTCAAGGTCGGCGACACTGTCGAGGCTGATCAGTCGCTGATCACCCTGGAATCCGACAAGGCGAGCATGGAA
ATCCCGTCGCCTGCCGCTGGCGTGGTCAAGGCTATCAGCGTCAAGTTGAACGACGAAGTCGGCACCGGCGACCTGATCCT
GGATCTGGAAGTGGCGGGTGCTGCGGCCCCTGCGGCTGCCGCTCCGGCCCAGGCTGCTGCGCCAGCCGCTGCGCCGGCGC
CGGCTGCTGCACCTGCCGCACCGGTTGCCGACAGCGTTCAGGACATCCACGTTCCGGACATCGGCTCGGCCGGCAAGGCC
AAGATCATCGAAGTGTTGGTCAAGGCTGGCGACAGCGTTGAAGCCGACCAGTCGCTGATCACCCTGGAATCCGACAAGGC
GAGCATGGAAATCCCGTCGCCTGCCGCCGGCGTGGTGGAAAGCATTTCCATCAAGCTGGACGACGAAGTCGGCACTGGCG
ATCTGATCCTCAAGCTGAAAGTCAAAGGCGCCGCTCCGGCTGCTGCCCCGGCTCCAGCTGCCGCTGCTGCTCCGAGCGCT
CCGGCGCCAGCTGCTGCTCCGGCTGCCGCTGCACCTGCTGCTGCCGCTCCGGTTGCTGCTCCAGCCAAGCCTGGTGCGAA
AGTTCACGCCGGCCCTGCCGTGCGTCAACTGGCTCGCGAGTTCGGCGTCGAGCTGAGCGCTGTCGGCGCCAGCGGTCCGC
ACGGTCGCATCCTGAAAGAAGACGTGCAGACCTACGTCAAAGCCATGATGCAGAAGGCCAAGGAAGCACCGGCCGCGGCT
GCTGGCGCAACCGGTGGCGCGGGCATCCCGCCGATTCCGGTCGTCGACTTCAGCCGTTTCGGCGAAATCGAAGAAGTGCC
GATGACCCGCCTGATGCAGGTCGGCGCTGCCAACCTGCACCGCAGCTGGCTTAACGTGCCGCACGTGACGCAATTCGACT
CGGCGGATATCACCGAGCTGGAGGCGTTCCGCACCGCACAGAAATCTGTCGCAGAGAAGGCTGGCGTCAAGCTGACCATT
CTGCCGCTGCTGCTCAAGTCCTGCGCACACCTGCTCAAGGAACTGCCGGACTTCAACAGCTCGCTGGCGCCAAGCGGCAA
GGCGATCATTCGCAAGAAGTACGTGAACATCGGCTTCGCCGTCGACACCCCGGATGGCCTGCTGGTACCGGTCATCAAGA
ACGTCGACCAGAAGAGCCTGCTGCAACTGGCCGCCGAAGCCGCTTCCCTGGCTGAAAAAGCCCGGACCAAGAAGCTCTCG
TCGGACGAGATGCAAGGCGCCTGCTTCACCATTTCCAGCCTCGGCCACATTGGCGGCACCGGCTTCACGCCGATCGTCAA
CGCGCCGGAAGTGGCGATCCTTGGTGTTTCCAAGGCAACCATCCAGCCAGTCTGGGACGGCAAAGCCTTCCAGCCGAAAC
TGATGCTGCCACTGTCGCTGTCCTACGATCACCGCGTGATCAACGGCGCCGCTGCTGCACGCTTCACCAAGCGTCTGAGC
GACCTGCTGGCGGACATCCGCACCATCCTGCTGTAA

Upstream 100 bases:

>100_bases
ACCGTGGTGATATCGAACCTAAAGTCGTGGCCGAGGCCATTACCAAGTTCGGCATCGACCCGGAAAAACGCAACCCACTG
GACTGCTGAGGAGAAACTCT

Downstream 100 bases:

>100_bases
AGCGCTCGGCCCTCCGGCAACGGAGGGCCGACCGCGCTCCACGTTTTCGAGCGCCACACGCTCGTACCTCAACCCCGTCA
GTTTGGCGGGGCTTTTTTTT

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 651; Mature: 650

Protein sequence:

>651_residues
MSELIRVPDIGSGEGEVIELFVKVGDRIEADQSILTLESDKASMEVPAPKAGVIKSLKVKLGDRLKEGDELLELEVEGAA
QAAPAPAAAPAAKAEAAPAAAPAPAAPAAAPAAASVQQVHVPDIGSSGKAQIIEIQVKVGDTVEADQSLITLESDKASME
IPSPAAGVVKAISVKLNDEVGTGDLILDLEVAGAAAPAAAAPAQAAAPAAAPAPAAAPAAPVADSVQDIHVPDIGSAGKA
KIIEVLVKAGDSVEADQSLITLESDKASMEIPSPAAGVVESISIKLDDEVGTGDLILKLKVKGAAPAAAPAPAAAAAPSA
PAPAAAPAAAAPAAAAPVAAPAKPGAKVHAGPAVRQLAREFGVELSAVGASGPHGRILKEDVQTYVKAMMQKAKEAPAAA
AGATGGAGIPPIPVVDFSRFGEIEEVPMTRLMQVGAANLHRSWLNVPHVTQFDSADITELEAFRTAQKSVAEKAGVKLTI
LPLLLKSCAHLLKELPDFNSSLAPSGKAIIRKKYVNIGFAVDTPDGLLVPVIKNVDQKSLLQLAAEAASLAEKARTKKLS
SDEMQGACFTISSLGHIGGTGFTPIVNAPEVAILGVSKATIQPVWDGKAFQPKLMLPLSLSYDHRVINGAAAARFTKRLS
DLLADIRTILL

Sequences:

>Translated_651_residues
MSELIRVPDIGSGEGEVIELFVKVGDRIEADQSILTLESDKASMEVPAPKAGVIKSLKVKLGDRLKEGDELLELEVEGAA
QAAPAPAAAPAAKAEAAPAAAPAPAAPAAAPAAASVQQVHVPDIGSSGKAQIIEIQVKVGDTVEADQSLITLESDKASME
IPSPAAGVVKAISVKLNDEVGTGDLILDLEVAGAAAPAAAAPAQAAAPAAAPAPAAAPAAPVADSVQDIHVPDIGSAGKA
KIIEVLVKAGDSVEADQSLITLESDKASMEIPSPAAGVVESISIKLDDEVGTGDLILKLKVKGAAPAAAPAPAAAAAPSA
PAPAAAPAAAAPAAAAPVAAPAKPGAKVHAGPAVRQLAREFGVELSAVGASGPHGRILKEDVQTYVKAMMQKAKEAPAAA
AGATGGAGIPPIPVVDFSRFGEIEEVPMTRLMQVGAANLHRSWLNVPHVTQFDSADITELEAFRTAQKSVAEKAGVKLTI
LPLLLKSCAHLLKELPDFNSSLAPSGKAIIRKKYVNIGFAVDTPDGLLVPVIKNVDQKSLLQLAAEAASLAEKARTKKLS
SDEMQGACFTISSLGHIGGTGFTPIVNAPEVAILGVSKATIQPVWDGKAFQPKLMLPLSLSYDHRVINGAAAARFTKRLS
DLLADIRTILL
>Mature_650_residues
SELIRVPDIGSGEGEVIELFVKVGDRIEADQSILTLESDKASMEVPAPKAGVIKSLKVKLGDRLKEGDELLELEVEGAAQ
AAPAPAAAPAAKAEAAPAAAPAPAAPAAAPAAASVQQVHVPDIGSSGKAQIIEIQVKVGDTVEADQSLITLESDKASMEI
PSPAAGVVKAISVKLNDEVGTGDLILDLEVAGAAAPAAAAPAQAAAPAAAPAPAAAPAAPVADSVQDIHVPDIGSAGKAK
IIEVLVKAGDSVEADQSLITLESDKASMEIPSPAAGVVESISIKLDDEVGTGDLILKLKVKGAAPAAAPAPAAAAAPSAP
APAAAPAAAAPAAAAPVAAPAKPGAKVHAGPAVRQLAREFGVELSAVGASGPHGRILKEDVQTYVKAMMQKAKEAPAAAA
GATGGAGIPPIPVVDFSRFGEIEEVPMTRLMQVGAANLHRSWLNVPHVTQFDSADITELEAFRTAQKSVAEKAGVKLTIL
PLLLKSCAHLLKELPDFNSSLAPSGKAIIRKKYVNIGFAVDTPDGLLVPVIKNVDQKSLLQLAAEAASLAEKARTKKLSS
DEMQGACFTISSLGHIGGTGFTPIVNAPEVAILGVSKATIQPVWDGKAFQPKLMLPLSLSYDHRVINGAAAARFTKRLSD
LLADIRTILL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=465, Percent_Identity=29.6774193548387, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI31711992, Length=328, Percent_Identity=33.2317073170732, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=32.8888888888889, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI203098816, Length=486, Percent_Identity=27.1604938271605, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI203098753, Length=431, Percent_Identity=27.6102088167053, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI260898739, Length=151, Percent_Identity=34.4370860927152, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1786305, Length=649, Percent_Identity=53.4668721109399, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI1786946, Length=426, Percent_Identity=28.6384976525822, Blast_Score=150, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=29.4811320754717, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=434, Percent_Identity=29.9539170506912, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25146366, Length=206, Percent_Identity=35.9223300970874, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=30.8196721311475, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=430, Percent_Identity=29.3023255813954, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324258, Length=431, Percent_Identity=27.6102088167053, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI18859875, Length=439, Percent_Identity=29.3849658314351, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=32.618025751073, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=32.618025751073, Blast_Score=122, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24645909, Length=217, Percent_Identity=32.258064516129, Blast_Score=119, Evalue=6e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 65956; Mature: 65825

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELIRVPDIGSGEGEVIELFVKVGDRIEADQSILTLESDKASMEVPAPKAGVIKSLKVK
CCCCEECCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCHHHHHHHHHH
LGDRLKEGDELLELEVEGAAQAAPAPAAAPAAKAEAAPAAAPAPAAPAAAPAAASVQQVH
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHCCEEEE
VPDIGSSGKAQIIEIQVKVGDTVEADQSLITLESDKASMEIPSPAAGVVKAISVKLNDEV
CCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCHHHHEEEEEEEECCCC
GTGDLILDLEVAGAAAPAAAAPAQAAAPAAAPAPAAAPAAPVADSVQDIHVPDIGSAGKA
CCCCEEEEEEECCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHCHHHCCCCCCCCCCHH
KIIEVLVKAGDSVEADQSLITLESDKASMEIPSPAAGVVESISIKLDDEVGTGDLILKLK
HHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHEEEEEEECCCCCCCCEEEEEE
VKGAAPAAAPAPAAAAAPSAPAPAAAPAAAAPAAAAPVAAPAKPGAKVHAGPAVRQLARE
ECCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHH
FGVELSAVGASGPHGRILKEDVQTYVKAMMQKAKEAPAAAAGATGGAGIPPIPVVDFSRF
HCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHC
GEIEEVPMTRLMQVGAANLHRSWLNVPHVTQFDSADITELEAFRTAQKSVAEKAGVKLTI
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEH
LPLLLKSCAHLLKELPDFNSSLAPSGKAIIRKKYVNIGFAVDTPDGLLVPVIKNVDQKSL
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEEEEEEEEEECCCCEEEHHHHCCCHHHH
LQLAAEAASLAEKARTKKLSSDEMQGACFTISSLGHIGGTGFTPIVNAPEVAILGVSKAT
HHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEHHHCCCCCCCCCCCCCCCCEEEEECCHHE
IQPVWDGKAFQPKLMLPLSLSYDHRVINGAAAARFTKRLSDLLADIRTILL
ECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SELIRVPDIGSGEGEVIELFVKVGDRIEADQSILTLESDKASMEVPAPKAGVIKSLKVK
CCCEECCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCHHHHHHHHHH
LGDRLKEGDELLELEVEGAAQAAPAPAAAPAAKAEAAPAAAPAPAAPAAAPAAASVQQVH
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHCCEEEE
VPDIGSSGKAQIIEIQVKVGDTVEADQSLITLESDKASMEIPSPAAGVVKAISVKLNDEV
CCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCHHHHEEEEEEEECCCC
GTGDLILDLEVAGAAAPAAAAPAQAAAPAAAPAPAAAPAAPVADSVQDIHVPDIGSAGKA
CCCCEEEEEEECCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHCHHHCCCCCCCCCCHH
KIIEVLVKAGDSVEADQSLITLESDKASMEIPSPAAGVVESISIKLDDEVGTGDLILKLK
HHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHEEEEEEECCCCCCCCEEEEEE
VKGAAPAAAPAPAAAAAPSAPAPAAAPAAAAPAAAAPVAAPAKPGAKVHAGPAVRQLARE
ECCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHH
FGVELSAVGASGPHGRILKEDVQTYVKAMMQKAKEAPAAAAGATGGAGIPPIPVVDFSRF
HCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHC
GEIEEVPMTRLMQVGAANLHRSWLNVPHVTQFDSADITELEAFRTAQKSVAEKAGVKLTI
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEH
LPLLLKSCAHLLKELPDFNSSLAPSGKAIIRKKYVNIGFAVDTPDGLLVPVIKNVDQKSL
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEEEEEEEEEECCCCEEEHHHHCCCHHHH
LQLAAEAASLAEKARTKKLSSDEMQGACFTISSLGHIGGTGFTPIVNAPEVAILGVSKAT
HHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEHHHCCCCCCCCCCCCCCCCEEEEECCHHE
IQPVWDGKAFQPKLMLPLSLSYDHRVINGAAAARFTKRLSDLLADIRTILL
ECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3292237; 3691494; 3191993; 1549782; 8068086; 9119000 [H]