Click here to switch to the map view.

The map label for this gene is srfAC [H]

Identifier: 77456664

GI number: 77456664

Start: 495234

End: 496913

Strand: Reverse

Name: srfAC [H]

Synonym: Pfl01_0436

Alternate gene names: 77456664

Gene position: 496913-495234 (Counterclockwise)

Preceding gene: 77456665

Following gene: 77456663

Centisome position: 7.72

GC content: 65.71

Gene sequence:

>1680_bases
ATGAACTGGATAAAACTTGAGCAGCTGTTGCTCAAGGCCCTGCCGGCGCGCGCAGTAACAAGCGCACCGGCACTCGATCA
CGCACAACTGCGCGAACAGGCCCTGAGCCTGGCCGCCGGTCTGCAAGCGCGCGGCGTGCAACGTCTGGCGGTGCATCTGG
AAGATGCCGCCGACCTGGCCGTCGCCCTGCTCGGCGCCTGGCGTGCCGGCGTCAGCGTGTTGCTGCCTTCAGATCTGCAA
GCGCAGACCCGTCAGCGCTGGTCGAACGACGTCGATCTGTGGCTGACCGATCATTCCGATGACGCTCGCCTGAGCGATCT
GCAGCAACCGGCCCTGCCCGCCGCCGAACTGGATCTCGACCAGTGCCGCCTCAGCCTGTGCACCTCCGGTTCCAGCGGCG
AGCCAAAGCGCATCGACAAATCCCTGCGGCAACTGGCCAACGAAGTCGAAGCCCTCGAACAGCTGTGGGGTGCGGATCTC
GGTGAAGCCTGCATTCTTGGCAGCGTTGCCACCCAGCACATCTACGGCCTGCTGTTCCGCGTGCTGTGGCCGCTGTGCGC
CGGGCGTCCGTTCCTGCGCAAGCAACTGGCCTTCCCGGAAGACTTGCAACGCGCCAGCCGCGAGCATCCGGCCTTCGCCT
GGGTCGCCAGCCCGGCGCTGCTCAAACGCATGGGCGACAACCTCGACTGGCCAGCGCTGAGCGCCGTGCGCCGGGTGTTT
TCCTCTGGAGGCGCATTGCCGACAGACGCCGCAAAAAGCCTGCATCAACGCTTGGGGCAGTGGCCGACGGAAATCCTCGG
CAGCTCGGAAACCGGCGGCATCGCCTGGCGCCAAGGCGAATCGTTGTGGCAGCCGTTTGCCGGTATCGAGTTGAGCCAGG
ACAGCGACGGCGCCCTGTTGATCGCTTCGCCTTACCTGCCCGCCGGGCAGGTCGAACACACCGCCGATGCGGCGCGCATT
CAGGCCGATGGCCGCTTTGAACTGCTCGGGCGGCTCGACCGGATCGTCAAACTCGAAGAAAAACGCATATCCCTGCCGAT
GCTTGAACAGGCGCTGGTGGCCCACGACTGGGTCGCCGAAGCGCGTCTGGGCGTGGTGCAGGAAAACCGCGCGTCGCTCG
GCGCTCTGCTGGTGCTCAGCGAATCGGGCCTGTTTGCCCTGCGCGAACACGGTCGGCGCAGCCTGACCGAAACCCTGCGC
CGTCATCTCGGCGACCACTGCGAAGCCCTGGCCCTGCCCCGCCGCTGGCGCCTGCTGCGGCAAATGCCGCTGACCTCGCA
GGGCAAGTTGCCTCAAGCCGACGTCGAAGCCTTGCTGCTGGCACCACGACCGAAAGCGCCGGAAATCCTGGAACAGGCCG
AAACCGAGGGTGAATGGAACCTGCAATTGAGCGTTCCGCCGGACCTTGCCTACTTCAGCGGCCACTTCCCCAAGGCCCCG
GTGCTGCCCGGCGTGGTGCAGGTCGAATGGGCACTGAACCTGGGTCGGCAACTGCTGAACCTGCCCGGCGCGTTCGCCGG
CATGGAAGTGCTGAAGTTCCAGCAACTGGTGCGCCCCGGCGATGAAATCCAGCTGCATCTGCGCTTCGATCCGGAACGCG
GCAAGTTGTATTTCGCCTACCGCAACGACACCGCCACCTGCTCCAGCGGGCGGATTCTGCTGGGGGCTGGCGATGCATAA

Upstream 100 bases:

>100_bases
TGAACTGGTGGATGTTGTACACCGGCCTGATCTCCTACGGATTGATCGGCCTGATGTTTGCCATTGAATGGCTGATACGA
CAACGGGTAAGAGGTCGCCC

Downstream 100 bases:

>100_bases
CCCTTGCGCCGTGATTCCGGTCTACAACCACGAAACCGCCATCGGCACGGTGGTCGACGCCCTGCTCGCCGAGGATCTGC
CGTGCATTCTGGTGGACGAT

Product: AMP-dependent synthetase and ligase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MNWIKLEQLLLKALPARAVTSAPALDHAQLREQALSLAAGLQARGVQRLAVHLEDAADLAVALLGAWRAGVSVLLPSDLQ
AQTRQRWSNDVDLWLTDHSDDARLSDLQQPALPAAELDLDQCRLSLCTSGSSGEPKRIDKSLRQLANEVEALEQLWGADL
GEACILGSVATQHIYGLLFRVLWPLCAGRPFLRKQLAFPEDLQRASREHPAFAWVASPALLKRMGDNLDWPALSAVRRVF
SSGGALPTDAAKSLHQRLGQWPTEILGSSETGGIAWRQGESLWQPFAGIELSQDSDGALLIASPYLPAGQVEHTADAARI
QADGRFELLGRLDRIVKLEEKRISLPMLEQALVAHDWVAEARLGVVQENRASLGALLVLSESGLFALREHGRRSLTETLR
RHLGDHCEALALPRRWRLLRQMPLTSQGKLPQADVEALLLAPRPKAPEILEQAETEGEWNLQLSVPPDLAYFSGHFPKAP
VLPGVVQVEWALNLGRQLLNLPGAFAGMEVLKFQQLVRPGDEIQLHLRFDPERGKLYFAYRNDTATCSSGRILLGAGDA

Sequences:

>Translated_559_residues
MNWIKLEQLLLKALPARAVTSAPALDHAQLREQALSLAAGLQARGVQRLAVHLEDAADLAVALLGAWRAGVSVLLPSDLQ
AQTRQRWSNDVDLWLTDHSDDARLSDLQQPALPAAELDLDQCRLSLCTSGSSGEPKRIDKSLRQLANEVEALEQLWGADL
GEACILGSVATQHIYGLLFRVLWPLCAGRPFLRKQLAFPEDLQRASREHPAFAWVASPALLKRMGDNLDWPALSAVRRVF
SSGGALPTDAAKSLHQRLGQWPTEILGSSETGGIAWRQGESLWQPFAGIELSQDSDGALLIASPYLPAGQVEHTADAARI
QADGRFELLGRLDRIVKLEEKRISLPMLEQALVAHDWVAEARLGVVQENRASLGALLVLSESGLFALREHGRRSLTETLR
RHLGDHCEALALPRRWRLLRQMPLTSQGKLPQADVEALLLAPRPKAPEILEQAETEGEWNLQLSVPPDLAYFSGHFPKAP
VLPGVVQVEWALNLGRQLLNLPGAFAGMEVLKFQQLVRPGDEIQLHLRFDPERGKLYFAYRNDTATCSSGRILLGAGDA
>Mature_559_residues
MNWIKLEQLLLKALPARAVTSAPALDHAQLREQALSLAAGLQARGVQRLAVHLEDAADLAVALLGAWRAGVSVLLPSDLQ
AQTRQRWSNDVDLWLTDHSDDARLSDLQQPALPAAELDLDQCRLSLCTSGSSGEPKRIDKSLRQLANEVEALEQLWGADL
GEACILGSVATQHIYGLLFRVLWPLCAGRPFLRKQLAFPEDLQRASREHPAFAWVASPALLKRMGDNLDWPALSAVRRVF
SSGGALPTDAAKSLHQRLGQWPTEILGSSETGGIAWRQGESLWQPFAGIELSQDSDGALLIASPYLPAGQVEHTADAARI
QADGRFELLGRLDRIVKLEEKRISLPMLEQALVAHDWVAEARLGVVQENRASLGALLVLSESGLFALREHGRRSLTETLR
RHLGDHCEALALPRRWRLLRQMPLTSQGKLPQADVEALLLAPRPKAPEILEQAETEGEWNLQLSVPPDLAYFSGHFPKAP
VLPGVVQVEWALNLGRQLLNLPGAFAGMEVLKFQQLVRPGDEIQLHLRFDPERGKLYFAYRNDTATCSSGRILLGAGDA

Specific function: Probably activates a leucine [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR006162
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 61421; Mature: 61421

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWIKLEQLLLKALPARAVTSAPALDHAQLREQALSLAAGLQARGVQRLAVHLEDAADLA
CCHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VALLGAWRAGVSVLLPSDLQAQTRQRWSNDVDLWLTDHSDDARLSDLQQPALPAAELDLD
HHHHHHHHHCCEEECCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCHHHCCHH
QCRLSLCTSGSSGEPKRIDKSLRQLANEVEALEQLWGADLGEACILGSVATQHIYGLLFR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VLWPLCAGRPFLRKQLAFPEDLQRASREHPAFAWVASPALLKRMGDNLDWPALSAVRRVF
HHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCEEEHHCHHHHHHHCCCCCCHHHHHHHHHH
SSGGALPTDAAKSLHQRLGQWPTEILGSSETGGIAWRQGESLWQPFAGIELSQDSDGALL
HCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCEEEECCHHHHCCCCCCEECCCCCCEEE
IASPYLPAGQVEHTADAARIQADGRFELLGRLDRIVKLEEKRISLPMLEQALVAHDWVAE
EECCCCCCCCCHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ARLGVVQENRASLGALLVLSESGLFALREHGRRSLTETLRRHLGDHCEALALPRRWRLLR
HHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCHHHHHHHH
QMPLTSQGKLPQADVEALLLAPRPKAPEILEQAETEGEWNLQLSVPPDLAYFSGHFPKAP
HCCCCCCCCCCHHCCCEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCCC
VLPGVVQVEWALNLGRQLLNLPGAFAGMEVLKFQQLVRPGDEIQLHLRFDPERGKLYFAY
CCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEE
RNDTATCSSGRILLGAGDA
CCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MNWIKLEQLLLKALPARAVTSAPALDHAQLREQALSLAAGLQARGVQRLAVHLEDAADLA
CCHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VALLGAWRAGVSVLLPSDLQAQTRQRWSNDVDLWLTDHSDDARLSDLQQPALPAAELDLD
HHHHHHHHHCCEEECCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCHHHCCHH
QCRLSLCTSGSSGEPKRIDKSLRQLANEVEALEQLWGADLGEACILGSVATQHIYGLLFR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VLWPLCAGRPFLRKQLAFPEDLQRASREHPAFAWVASPALLKRMGDNLDWPALSAVRRVF
HHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCEEEHHCHHHHHHHCCCCCCHHHHHHHHHH
SSGGALPTDAAKSLHQRLGQWPTEILGSSETGGIAWRQGESLWQPFAGIELSQDSDGALL
HCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCEEEECCHHHHCCCCCCEECCCCCCEEE
IASPYLPAGQVEHTADAARIQADGRFELLGRLDRIVKLEEKRISLPMLEQALVAHDWVAE
EECCCCCCCCCHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ARLGVVQENRASLGALLVLSESGLFALREHGRRSLTETLRRHLGDHCEALALPRRWRLLR
HHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCHHHHHHHH
QMPLTSQGKLPQADVEALLLAPRPKAPEILEQAETEGEWNLQLSVPPDLAYFSGHFPKAP
HCCCCCCCCCCHHCCCEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCCC
VLPGVVQVEWALNLGRQLLNLPGAFAGMEVLKFQQLVRPGDEIQLHLRFDPERGKLYFAY
CCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEE
RNDTATCSSGRILLGAGDA
CCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8355609; 8969502; 9384377; 12005429 [H]