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The map label for this gene is phaC [H]

Identifier: 77456620

GI number: 77456620

Start: 444113

End: 445795

Strand: Reverse

Name: phaC [H]

Synonym: Pfl01_0392

Alternate gene names: 77456620

Gene position: 445795-444113 (Counterclockwise)

Preceding gene: 77456621

Following gene: 77456619

Centisome position: 6.92

GC content: 62.45

Gene sequence:

>1683_bases
ATGCGTGACAAACCAGCGACGGGCGTCGTGCCCAGCCCCGCCGTGTTCATCAATGCACAGAGTGCCATGACCGGTCTGCG
TGGCCGCGACCTGATCTCGACCTTGCGCAGCGTGGCAGCCCACGGCTTGCGCAATCCGATCCACAGTGCAAAACACGCCT
TGAAGCTGGGAGGTGCGCTCGGACGCGTGCTGCTCGGCGAAACCCTGCACCCGACCAATCCGCAGGACAGCCGCTTCGCC
GATCCGGCGTGGAGCCTGAACCCGTTCTATCGGCGCAGCCTGCAGGCTTATCTGGCCTGGCAGAAACAGGTCAAGAGCTG
GATCGACGAGAGCAGCATGAGTGACGACGACCGCGCCCGCGCGCATTTCGCCTTCACCCTGCTCAACGACGCCGTGGCGC
CTTCCAACACCCTGCTCAATCCGCTGGCTGTGAAAGAACTGTTCAACTCCGGCGGCCACAGTCTGGTGCGCGGTCTCAGC
CATCTGATTGATGACCTGCTGCACAACGACGGCCTGCCACGCCAGGTCACCAAACAAGCTTTCGAAGTCGGCAAGACCGT
CGCCACCACCACCGGCTCGGTGGTGTTTCGCAATGAAATGCTCGAGCTGATCCAGTACCGGCCGATGAGCGAAAAACAGT
ATTCGAAACCGTTGCTGGTGGTGCCGCCGCAAATCAACAAGTACTACATTTTCGACCTCAGCCCGAGCAACAGCTTCGTC
CAGTTCGCCCTGAAGAACGGTTTGCAGACCTTCATGATCAGCTGGCGCAACCCGGATGTGCGGCATCGCGAATGGGGCCT
CTCGACCTACGTCGAAGCCGTGGAGGAAGCGATGAACATCTGCCGGGCGATCACCGGCGCCCGTGAGGTCAACCTGATGG
GCGCCTGCGCCGGCGGGCTGACCATCGCGGCACTGCAAGGGCATCTGCAGGCCAAGCGGCAGTTGCGGCGAGTCTCCAGC
GCCACCTATCTGGTGAGCCTGCTCGACAGTGAGATGAACACCCCGGCCACCCTGTTCGCCGATGAGCAAACGCTCGAAGC
GGCCAAGCGGCGTTCCTATCAGAAAGGCGTGCTGGACGGCCGCGACATGGCCAAGGTGTTCGCCTGGATGCGCCCCAACG
ATTTGATCTGGAGTTATTTCGTCAACAACTATCTGCTGGGCAAGGAGCCGCCGGCGTTCGACATTCTCTACTGGAACAAC
GACAGCACCCGCCTGCCCGCCGCGTTTCACGGCGACATTCTGGACTTCTTCAAGCACAACCCGTTGACCCATCCGGGGGG
CCTGGAAGTGTGCGGCACGCCGATCGACCTGCAGAAGGTCACGGTCGACAGTTTCAGCGTGGCCGGCATGAACGACCACA
TCACGCCGTGGGACGCGGTGTATCGCTCGACACTGTTGCTGGGCGGCGAGCGGCGCTTCGTGCTGTCCAACAGCGGCCAC
GTGCAGAGCATCCTCAACCCGCCGGGCAACCCGAAAGCCCATTACGTCGAGAACGGAAAGCTCAGCAGCGATCCGCGCGC
CTGGTACTACGACGCCAAGAAAGTCGACGGCAGCTGGTGGCCGCAATGGCTGGAGTGGGTGCAGCAGCGCTCCGGCACCC
TGCACGAAACCCGGATGACCCTCGGCAACGCCAATTATCCACCGATGGAAGCGGCACCCGGCACCTATGTGCGTGTGCGC
TGA

Upstream 100 bases:

>100_bases
GCGTGGCAGGAGGCCGCGCAAGCAGCAACCCGCAACAGCGTCTATGGTGTTCTGGTTCGGTGTGTTTGTTTTTGGCCTGA
AGACGAAGGAGTGTTGAGTC

Downstream 100 bases:

>100_bases
CTGACCCAAGCCCTTTTAAGAAGACTGGATGAAAACAAGCGAACGGATTCTCGAATGTGCCCTGCAGTTGTTCAATGAAA
AGGGCGAGCCGAACGTCTCC

Product: Poly(R)-hydroxyalkanoic acid synthase, class II

Products: NA

Alternate protein names: PHA polymerase 2; PHA synthase 2; Polyhydroxyalkanoic acid synthase 2 [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MRDKPATGVVPSPAVFINAQSAMTGLRGRDLISTLRSVAAHGLRNPIHSAKHALKLGGALGRVLLGETLHPTNPQDSRFA
DPAWSLNPFYRRSLQAYLAWQKQVKSWIDESSMSDDDRARAHFAFTLLNDAVAPSNTLLNPLAVKELFNSGGHSLVRGLS
HLIDDLLHNDGLPRQVTKQAFEVGKTVATTTGSVVFRNEMLELIQYRPMSEKQYSKPLLVVPPQINKYYIFDLSPSNSFV
QFALKNGLQTFMISWRNPDVRHREWGLSTYVEAVEEAMNICRAITGAREVNLMGACAGGLTIAALQGHLQAKRQLRRVSS
ATYLVSLLDSEMNTPATLFADEQTLEAAKRRSYQKGVLDGRDMAKVFAWMRPNDLIWSYFVNNYLLGKEPPAFDILYWNN
DSTRLPAAFHGDILDFFKHNPLTHPGGLEVCGTPIDLQKVTVDSFSVAGMNDHITPWDAVYRSTLLLGGERRFVLSNSGH
VQSILNPPGNPKAHYVENGKLSSDPRAWYYDAKKVDGSWWPQWLEWVQQRSGTLHETRMTLGNANYPPMEAAPGTYVRVR

Sequences:

>Translated_560_residues
MRDKPATGVVPSPAVFINAQSAMTGLRGRDLISTLRSVAAHGLRNPIHSAKHALKLGGALGRVLLGETLHPTNPQDSRFA
DPAWSLNPFYRRSLQAYLAWQKQVKSWIDESSMSDDDRARAHFAFTLLNDAVAPSNTLLNPLAVKELFNSGGHSLVRGLS
HLIDDLLHNDGLPRQVTKQAFEVGKTVATTTGSVVFRNEMLELIQYRPMSEKQYSKPLLVVPPQINKYYIFDLSPSNSFV
QFALKNGLQTFMISWRNPDVRHREWGLSTYVEAVEEAMNICRAITGAREVNLMGACAGGLTIAALQGHLQAKRQLRRVSS
ATYLVSLLDSEMNTPATLFADEQTLEAAKRRSYQKGVLDGRDMAKVFAWMRPNDLIWSYFVNNYLLGKEPPAFDILYWNN
DSTRLPAAFHGDILDFFKHNPLTHPGGLEVCGTPIDLQKVTVDSFSVAGMNDHITPWDAVYRSTLLLGGERRFVLSNSGH
VQSILNPPGNPKAHYVENGKLSSDPRAWYYDAKKVDGSWWPQWLEWVQQRSGTLHETRMTLGNANYPPMEAAPGTYVRVR
>Mature_560_residues
MRDKPATGVVPSPAVFINAQSAMTGLRGRDLISTLRSVAAHGLRNPIHSAKHALKLGGALGRVLLGETLHPTNPQDSRFA
DPAWSLNPFYRRSLQAYLAWQKQVKSWIDESSMSDDDRARAHFAFTLLNDAVAPSNTLLNPLAVKELFNSGGHSLVRGLS
HLIDDLLHNDGLPRQVTKQAFEVGKTVATTTGSVVFRNEMLELIQYRPMSEKQYSKPLLVVPPQINKYYIFDLSPSNSFV
QFALKNGLQTFMISWRNPDVRHREWGLSTYVEAVEEAMNICRAITGAREVNLMGACAGGLTIAALQGHLQAKRQLRRVSS
ATYLVSLLDSEMNTPATLFADEQTLEAAKRRSYQKGVLDGRDMAKVFAWMRPNDLIWSYFVNNYLLGKEPPAFDILYWNN
DSTRLPAAFHGDILDFFKHNPLTHPGGLEVCGTPIDLQKVTVDSFSVAGMNDHITPWDAVYRSTLLLGGERRFVLSNSGH
VQSILNPPGNPKAHYVENGKLSSDPRAWYYDAKKVDGSWWPQWLEWVQQRSGTLHETRMTLGNANYPPMEAAPGTYVRVR

Specific function: P.oleovorans accumulates poly(3-hydroxyalkanoates) after growth on medium chain length hydrocarbons. Large amounts of this polyester are synthesized when cells are grown under nitrogen- limiting conditions. When nitrogen is resupplied in the medium, the a

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011287
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 62613; Mature: 62613

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDKPATGVVPSPAVFINAQSAMTGLRGRDLISTLRSVAAHGLRNPIHSAKHALKLGGAL
CCCCCCCCCCCCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GRVLLGETLHPTNPQDSRFADPAWSLNPFYRRSLQAYLAWQKQVKSWIDESSMSDDDRAR
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AHFAFTLLNDAVAPSNTLLNPLAVKELFNSGGHSLVRGLSHLIDDLLHNDGLPRQVTKQA
HHEEEEHHHCCCCCCCHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FEVGKTVATTTGSVVFRNEMLELIQYRPMSEKQYSKPLLVVPPQINKYYIFDLSPSNSFV
HHHCCHHEECCCCHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCCEEEEEEECCCCHHH
QFALKNGLQTFMISWRNPDVRHREWGLSTYVEAVEEAMNICRAITGAREVNLMGACAGGL
HHHHHCCCEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCH
TIAALQGHLQAKRQLRRVSSATYLVSLLDSEMNTPATLFADEQTLEAAKRRSYQKGVLDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCC
RDMAKVFAWMRPNDLIWSYFVNNYLLGKEPPAFDILYWNNDSTRLPAAFHGDILDFFKHN
HHHHHHHHHCCCCHHHHHHHHHCEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCC
PLTHPGGLEVCGTPIDLQKVTVDSFSVAGMNDHITPWDAVYRSTLLLGGERRFVLSNSGH
CCCCCCCCCCCCCCCCCEEEEECCEEECCCCCCCCHHHHHHHHHHEECCCEEEEECCCCC
VQSILNPPGNPKAHYVENGKLSSDPRAWYYDAKKVDGSWWPQWLEWVQQRSGTLHETRMT
CHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHH
LGNANYPPMEAAPGTYVRVR
HCCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MRDKPATGVVPSPAVFINAQSAMTGLRGRDLISTLRSVAAHGLRNPIHSAKHALKLGGAL
CCCCCCCCCCCCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GRVLLGETLHPTNPQDSRFADPAWSLNPFYRRSLQAYLAWQKQVKSWIDESSMSDDDRAR
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AHFAFTLLNDAVAPSNTLLNPLAVKELFNSGGHSLVRGLSHLIDDLLHNDGLPRQVTKQA
HHEEEEHHHCCCCCCCHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FEVGKTVATTTGSVVFRNEMLELIQYRPMSEKQYSKPLLVVPPQINKYYIFDLSPSNSFV
HHHCCHHEECCCCHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCCEEEEEEECCCCHHH
QFALKNGLQTFMISWRNPDVRHREWGLSTYVEAVEEAMNICRAITGAREVNLMGACAGGL
HHHHHCCCEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCH
TIAALQGHLQAKRQLRRVSSATYLVSLLDSEMNTPATLFADEQTLEAAKRRSYQKGVLDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCC
RDMAKVFAWMRPNDLIWSYFVNNYLLGKEPPAFDILYWNNDSTRLPAAFHGDILDFFKHN
HHHHHHHHHCCCCHHHHHHHHHCEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCC
PLTHPGGLEVCGTPIDLQKVTVDSFSVAGMNDHITPWDAVYRSTLLLGGERRFVLSNSGH
CCCCCCCCCCCCCCCCCEEEEECCEEECCCCCCCCHHHHHHHHHHEECCCEEEEECCCCC
VQSILNPPGNPKAHYVENGKLSSDPRAWYYDAKKVDGSWWPQWLEWVQQRSGTLHETRMT
CHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHH
LGNANYPPMEAAPGTYVRVR
HCCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]