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The map label for this gene is hicB

Identifier: 77456533

GI number: 77456533

Start: 352128

End: 352568

Strand: Direct

Name: hicB

Synonym: Pfl01_0305

Alternate gene names: 77456533

Gene position: 352128-352568 (Clockwise)

Preceding gene: 77456532

Following gene: 77456534

Centisome position: 5.47

GC content: 56.24

Gene sequence:

>441_bases
ATGAGAGGAAGCGTGATGTTCGAATATGCGTTGGAAGTCCACGAAGAGCCGGGCAGTGTCTGGTTGTCCTGCGCAGAAAT
TCCAGAGATGCACGCCGCTGGCGACACCCTCGAGGAAGCACTCGACGGCGCTATAGATGCGATGGAAACGGCATTGTCGA
TTTACGTCGATGATCGCCGGTTGATTCCGACAGGTGATGCGGGAGAGCAGGAAAGTGACGCGGTATTGCGTCTGCCTGCA
CTGACTGCCGCAAAGGTCGGGTTGTGGAATACGCTTCTGGAGTCTGGGGTGAGCAAGGCTGAGCTGGCACGGCGCTTGGG
TGTGCAACGACCCCAGGTAGATCGGTTAGTCGATTTCCTGCATCACTCCAAGATTGAGAATGTTGAGCGTGCGTTGCAGC
AGCTTGGACGCCGGATTTTGCTTTCGGTTGAGGCTGCTTAG

Upstream 100 bases:

>100_bases
TGTCTTTCCCGACCACGGAGCCAAGGAGATGGGCGAAGGGTTGAGAAAATCGATAGTCAAACAGCTGGGCCTCAAGGATT
GAGGCCCAGCTGTACCCCTT

Downstream 100 bases:

>100_bases
CTTTATCAACTGAGCGTTGGAGAAAAGTAATTGGATGATGAAATAGCGGTGGTTAATGAACTGGAGCAATTCCAGCAGGA
CTTGCTGGACTCTGTCCGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MRGSVMFEYALEVHEEPGSVWLSCAEIPEMHAAGDTLEEALDGAIDAMETALSIYVDDRRLIPTGDAGEQESDAVLRLPA
LTAAKVGLWNTLLESGVSKAELARRLGVQRPQVDRLVDFLHHSKIENVERALQQLGRRILLSVEAA

Sequences:

>Translated_146_residues
MRGSVMFEYALEVHEEPGSVWLSCAEIPEMHAAGDTLEEALDGAIDAMETALSIYVDDRRLIPTGDAGEQESDAVLRLPA
LTAAKVGLWNTLLESGVSKAELARRLGVQRPQVDRLVDFLHHSKIENVERALQQLGRRILLSVEAA
>Mature_146_residues
MRGSVMFEYALEVHEEPGSVWLSCAEIPEMHAAGDTLEEALDGAIDAMETALSIYVDDRRLIPTGDAGEQESDAVLRLPA
LTAAKVGLWNTLLESGVSKAELARRLGVQRPQVDRLVDFLHHSKIENVERALQQLGRRILLSVEAA

Specific function: Part of a toxin-antitoxin (TA) module. Functions as an mRNA interferase antitoxin preventing effects of the HicA toxin

COG id: COG1598

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HicB antitoxin family

Homologues:

Organism=Escherichia coli, GI145693129, Length=124, Percent_Identity=35.4838709677419, Blast_Score=81, Evalue=2e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): HICB_PSEPF (Q3KJK7)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346038.1
- ProteinModelPortal:   Q3KJK7
- STRING:   Q3KJK7
- GeneID:   3713195
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_0305
- eggNOG:   COG1598
- HOGENOM:   HBG472676
- OMA:   CPDIPEM
- ProtClustDB:   CLSK757906
- BioCyc:   PFLU205922:PFL_0305-MONOMER
- InterPro:   IPR012287
- InterPro:   IPR005357
- Gene3D:   G3DSA:1.10.10.60

Pfam domain/function: PF03681 UPF0150

EC number: NA

Molecular weight: Translated: 16017; Mature: 16017

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGSVMFEYALEVHEEPGSVWLSCAEIPEMHAAGDTLEEALDGAIDAMETALSIYVDDRR
CCCCHHHHHHHHHHCCCCCEEEEHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHEECCCE
LIPTGDAGEQESDAVLRLPALTAAKVGLWNTLLESGVSKAELARRLGVQRPQVDRLVDFL
ECCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHH
HHSKIENVERALQQLGRRILLSVEAA
HHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRGSVMFEYALEVHEEPGSVWLSCAEIPEMHAAGDTLEEALDGAIDAMETALSIYVDDRR
CCCCHHHHHHHHHHCCCCCEEEEHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHEECCCE
LIPTGDAGEQESDAVLRLPALTAAKVGLWNTLLESGVSKAELARRLGVQRPQVDRLVDFL
ECCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHH
HHSKIENVERALQQLGRRILLSVEAA
HHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA