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The map label for this gene is flbD [H]

Identifier: 77456450

GI number: 77456450

Start: 263562

End: 264494

Strand: Direct

Name: flbD [H]

Synonym: Pfl01_0222

Alternate gene names: 77456450

Gene position: 263562-264494 (Clockwise)

Preceding gene: 77456449

Following gene: 77456451

Centisome position: 4.09

GC content: 60.99

Gene sequence:

>933_bases
ATGAGTCTTGAAACCTTCGGTCAGCCGTTGCTGACCTTTCCCGATGCGGAAAAAAGTCCCCTGAGCATTCGCGCCAAGGC
GCTGGTGTTCGTCGATCCACGCTCGCGCCAGTTGCGCGAAGAAATGGCCCAACTCGCACCGCGCTCGATCTCGGTGTTGA
TTCGCGGCGAGACCGGCACCGGCAAGGAATTACTCGCTCGCCATATCCATCGCGCCAGTGATCGCAGCGGATTGTTTGTC
TCGGTCAATTGCGGCGCGATCAGCCCGACCTACGCCGATGCGGAATTGTTCGGTTATGCCGCCGGCAGCTACAGCGGTTC
GGCCAGCAGTCGCGCCGGTTGGTTCGGTTCAGCCAACGGCGGCACCTTGTACTTGGACGAGATCGGCGACCTGCCGTTGC
CGATCCAGATCAAGTTGCTTTCCGCACTGGAAAACCACGAAGTCACCCGCGTCGGTGCGCATCAGCCGAGCCCGGTGGAC
GTGCGCCTGGTAGCGGCGACCAGCATCGATCTGGCGCAAGCGGTGGCGGCAGGGAAATTCCACGAGCGGCTTTATCACTA
TCTCAGCGAAGGGCATCTGGAACTGCCAGCGTTGCGCGCGCGGGTTGGCGACATTCTGTCGTTGGCCGAGTACTTCCTCG
GCATTTACAGCCAGCGTCTGGATCTGCCGGTGCCTTTGATCAGCGAAGCGGCGCAGCAGGTGCTGGAACAGCACAGTTGG
CCGGGCAACACCCGGGAGCTGGAAAACGTCATTCATTTTGCGTTGCTGGTGAGCACCGGCGACGAAATCCTGCCGGAGCA
CCTGAACCTGCCCGAGGTGTCTGGGCCGCAGGTTCAGATCGAGCGTCAGGTGGCGAGCGTCAGCCTCAACGGCACCGTCG
ACGAACGTAAGGCATTGAAAGAATGGCTGTTGAGCTTGAGCGAGGCGTTGTAA

Upstream 100 bases:

>100_bases
GCCTGCAAAACCGCATGGAACACCACGCCCGGCGCCGTGCCTGATCACTTTTATTTGTTCAACGCCCCCGTCTCCCGGCG
ATTTGGGGCGGGAGTCTGCC

Downstream 100 bases:

>100_bases
CCCTTTTGCTCAACATGAACAAAATGGAATATGAAGCTGAATAAACGTTATTGTCCGGGAATAAAAAATCCCGGTATTGT
CCGCTTCACGCCAGCAGTAG

Product: response regulator receiver domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSLETFGQPLLTFPDAEKSPLSIRAKALVFVDPRSRQLREEMAQLAPRSISVLIRGETGTGKELLARHIHRASDRSGLFV
SVNCGAISPTYADAELFGYAAGSYSGSASSRAGWFGSANGGTLYLDEIGDLPLPIQIKLLSALENHEVTRVGAHQPSPVD
VRLVAATSIDLAQAVAAGKFHERLYHYLSEGHLELPALRARVGDILSLAEYFLGIYSQRLDLPVPLISEAAQQVLEQHSW
PGNTRELENVIHFALLVSTGDEILPEHLNLPEVSGPQVQIERQVASVSLNGTVDERKALKEWLLSLSEAL

Sequences:

>Translated_310_residues
MSLETFGQPLLTFPDAEKSPLSIRAKALVFVDPRSRQLREEMAQLAPRSISVLIRGETGTGKELLARHIHRASDRSGLFV
SVNCGAISPTYADAELFGYAAGSYSGSASSRAGWFGSANGGTLYLDEIGDLPLPIQIKLLSALENHEVTRVGAHQPSPVD
VRLVAATSIDLAQAVAAGKFHERLYHYLSEGHLELPALRARVGDILSLAEYFLGIYSQRLDLPVPLISEAAQQVLEQHSW
PGNTRELENVIHFALLVSTGDEILPEHLNLPEVSGPQVQIERQVASVSLNGTVDERKALKEWLLSLSEAL
>Mature_309_residues
SLETFGQPLLTFPDAEKSPLSIRAKALVFVDPRSRQLREEMAQLAPRSISVLIRGETGTGKELLARHIHRASDRSGLFVS
VNCGAISPTYADAELFGYAAGSYSGSASSRAGWFGSANGGTLYLDEIGDLPLPIQIKLLSALENHEVTRVGAHQPSPVDV
RLVAATSIDLAQAVAAGKFHERLYHYLSEGHLELPALRARVGDILSLAEYFLGIYSQRLDLPVPLISEAAQQVLEQHSWP
GNTRELENVIHFALLVSTGDEILPEHLNLPEVSGPQVQIERQVASVSLNGTVDERKALKEWLLSLSEAL

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1786524, Length=261, Percent_Identity=45.2107279693487, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1789233, Length=255, Percent_Identity=41.1764705882353, Blast_Score=197, Evalue=5e-52,
Organism=Escherichia coli, GI1790437, Length=290, Percent_Identity=40, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI87082152, Length=266, Percent_Identity=40.9774436090226, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788550, Length=245, Percent_Identity=43.6734693877551, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1790299, Length=228, Percent_Identity=42.5438596491228, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1788905, Length=227, Percent_Identity=43.1718061674009, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87082117, Length=234, Percent_Identity=43.1623931623932, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI87081872, Length=214, Percent_Identity=44.392523364486, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1789087, Length=253, Percent_Identity=38.7351778656126, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1787583, Length=269, Percent_Identity=36.0594795539033, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1789828, Length=259, Percent_Identity=33.5907335907336, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081858, Length=264, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 33716; Mature: 33584

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLETFGQPLLTFPDAEKSPLSIRAKALVFVDPRSRQLREEMAQLAPRSISVLIRGETGT
CCCCHHCCCCEECCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCC
GKELLARHIHRASDRSGLFVSVNCGAISPTYADAELFGYAAGSYSGSASSRAGWFGSANG
CHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCC
GTLYLDEIGDLPLPIQIKLLSALENHEVTRVGAHQPSPVDVRLVAATSIDLAQAVAAGKF
CEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHH
HERLYHYLSEGHLELPALRARVGDILSLAEYFLGIYSQRLDLPVPLISEAAQQVLEQHSW
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
PGNTRELENVIHFALLVSTGDEILPEHLNLPEVSGPQVQIERQVASVSLNGTVDERKALK
CCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEEEHEEEEEECCCHHHHHHHH
EWLLSLSEAL
HHHHHHHHCC
>Mature Secondary Structure 
SLETFGQPLLTFPDAEKSPLSIRAKALVFVDPRSRQLREEMAQLAPRSISVLIRGETGT
CCCHHCCCCEECCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCC
GKELLARHIHRASDRSGLFVSVNCGAISPTYADAELFGYAAGSYSGSASSRAGWFGSANG
CHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCC
GTLYLDEIGDLPLPIQIKLLSALENHEVTRVGAHQPSPVDVRLVAATSIDLAQAVAAGKF
CEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHH
HERLYHYLSEGHLELPALRARVGDILSLAEYFLGIYSQRLDLPVPLISEAAQQVLEQHSW
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
PGNTRELENVIHFALLVSTGDEILPEHLNLPEVSGPQVQIERQVASVSLNGTVDERKALK
CCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEEEHEEEEEECCCHHHHHHHH
EWLLSLSEAL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]