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The map label for this gene is gcvA [H]

Identifier: 77456344

GI number: 77456344

Start: 117350

End: 118249

Strand: Reverse

Name: gcvA [H]

Synonym: Pfl01_0116

Alternate gene names: 77456344

Gene position: 118249-117350 (Counterclockwise)

Preceding gene: 77456347

Following gene: 77456342

Centisome position: 1.84

GC content: 60.22

Gene sequence:

>900_bases
ATGCGCCGCAAGATACCCAGCACCACCGCCCTGATCAGCTTCGAAGCCGCCGCCCGTCACGAGAGCTTTACCAAGGCCGC
CGAAGAACTTTCCCTCACTCAAGGCGCCATTTGCCGACAGATTGCCAGCCTGGAGGACTTCCTCAGCGTCGAACTGTTCC
GACGCTCGCGTCGCGGGGTGAAGCTGACAGAAGCGGGCCTTTCCTATAGTCGTCGGGTCGCCACGCAACTCGACGCCGTT
GAGCGCGACACCCTGTCGGTGATGGGTCAGCAAGGCACCAATGTGATCGAGCTCGCCGTGGTGCCCACCTTCGGCACGCA
ATGGCTGCTGCCAAGACTGAAGGACTTCCAGCTCAAGCATCCGGAGGTGACAGTCAACCTGACCAACCGCACGCGCCCGT
TCCTGTTTGCCGACACCGATTTCGACGCGGCGATCTACTTCGGCGACGCCGACTGGTCCGGTACCGAATCCCACAGGTTG
ATGGGCGAGAATCCGATGCCGGTGTGCAGTCCTGCCCTGCTCGGCAACAAGACGCATCTGACACCCGATGAGATCGCCGA
TCTGCCGCTGCTCCAGCAAACCACACGCCCGTACGCCTGGCGACAGTGGTTCAACTCGCAACACCTGAACATTCCCCGCG
ACATGACAGGTCCGCGCTACGAGCTATTCTCCATGCTGGCCCAGGCCGCCATGCACGACATGGGCATCGCGCTGATCCCA
CCATTCCTCATTCAGCGCGAACTGGCCGAGAAACGCCTGGTGATTGCCAACCCGAACGCGCTCTCCAGCATCAAGGCGTA
TTACCTGATGATTCCGGAGCGAAAGGTCGAATCAGCGTCACTCAAGGCTTTTCGCGATTGGTTAGTGAGCCAGGCACACA
GCTACAACCTAGAAGAATAA

Upstream 100 bases:

>100_bases
CATCCCGGCAAACGAGGTTTTCGCATCCTGTTGTGCTAATTTCTCACTCCGAAACGTCGCGAAATGCCGATTGAGCGATG
TATTAGTGAGGTTGACGTAC

Downstream 100 bases:

>100_bases
AGGGCTACAGCGATTGCTAACCCCGTAGTCAGGAAATCCAAAGCCCTACAGATATAACTATTTGTCGCAAATAGACAGAC
TGTATCCGAAAGTCGTACAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRRKIPSTTALISFEAAARHESFTKAAEELSLTQGAICRQIASLEDFLSVELFRRSRRGVKLTEAGLSYSRRVATQLDAV
ERDTLSVMGQQGTNVIELAVVPTFGTQWLLPRLKDFQLKHPEVTVNLTNRTRPFLFADTDFDAAIYFGDADWSGTESHRL
MGENPMPVCSPALLGNKTHLTPDEIADLPLLQQTTRPYAWRQWFNSQHLNIPRDMTGPRYELFSMLAQAAMHDMGIALIP
PFLIQRELAEKRLVIANPNALSSIKAYYLMIPERKVESASLKAFRDWLVSQAHSYNLEE

Sequences:

>Translated_299_residues
MRRKIPSTTALISFEAAARHESFTKAAEELSLTQGAICRQIASLEDFLSVELFRRSRRGVKLTEAGLSYSRRVATQLDAV
ERDTLSVMGQQGTNVIELAVVPTFGTQWLLPRLKDFQLKHPEVTVNLTNRTRPFLFADTDFDAAIYFGDADWSGTESHRL
MGENPMPVCSPALLGNKTHLTPDEIADLPLLQQTTRPYAWRQWFNSQHLNIPRDMTGPRYELFSMLAQAAMHDMGIALIP
PFLIQRELAEKRLVIANPNALSSIKAYYLMIPERKVESASLKAFRDWLVSQAHSYNLEE
>Mature_299_residues
MRRKIPSTTALISFEAAARHESFTKAAEELSLTQGAICRQIASLEDFLSVELFRRSRRGVKLTEAGLSYSRRVATQLDAV
ERDTLSVMGQQGTNVIELAVVPTFGTQWLLPRLKDFQLKHPEVTVNLTNRTRPFLFADTDFDAAIYFGDADWSGTESHRL
MGENPMPVCSPALLGNKTHLTPDEIADLPLLQQTTRPYAWRQWFNSQHLNIPRDMTGPRYELFSMLAQAAMHDMGIALIP
PFLIQRELAEKRLVIANPNALSSIKAYYLMIPERKVESASLKAFRDWLVSQAHSYNLEE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=35.8361774744027, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1786448, Length=283, Percent_Identity=31.4487632508834, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=29.757785467128, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=26.8211920529801, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787589, Length=301, Percent_Identity=22.9235880398671, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI157672245, Length=141, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=25.6944444444444, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789639, Length=276, Percent_Identity=27.8985507246377, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787128, Length=135, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33866; Mature: 33866

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50931 HTH_LYSR ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKIPSTTALISFEAAARHESFTKAAEELSLTQGAICRQIASLEDFLSVELFRRSRRGV
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KLTEAGLSYSRRVATQLDAVERDTLSVMGQQGTNVIELAVVPTFGTQWLLPRLKDFQLKH
EEEHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCHHCCCCCC
PEVTVNLTNRTRPFLFADTDFDAAIYFGDADWSGTESHRLMGENPMPVCSPALLGNKTHL
CEEEEEECCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCC
TPDEIADLPLLQQTTRPYAWRQWFNSQHLNIPRDMTGPRYELFSMLAQAAMHDMGIALIP
CHHHHHCCCHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHC
PFLIQRELAEKRLVIANPNALSSIKAYYLMIPERKVESASLKAFRDWLVSQAHSYNLEE
HHHHHHHHHHCEEEEECCCHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRRKIPSTTALISFEAAARHESFTKAAEELSLTQGAICRQIASLEDFLSVELFRRSRRGV
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KLTEAGLSYSRRVATQLDAVERDTLSVMGQQGTNVIELAVVPTFGTQWLLPRLKDFQLKH
EEEHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCHHCCCCCC
PEVTVNLTNRTRPFLFADTDFDAAIYFGDADWSGTESHRLMGENPMPVCSPALLGNKTHL
CEEEEEECCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCC
TPDEIADLPLLQQTTRPYAWRQWFNSQHLNIPRDMTGPRYELFSMLAQAAMHDMGIALIP
CHHHHHCCCHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHC
PFLIQRELAEKRLVIANPNALSSIKAYYLMIPERKVESASLKAFRDWLVSQAHSYNLEE
HHHHHHHHHHCEEEEECCCHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]