Click here to switch to the map view.

The map label for this gene is trpB

Identifier: 77456331

GI number: 77456331

Start: 105726

End: 106958

Strand: Direct

Name: trpB

Synonym: Pfl01_0103

Alternate gene names: 77456331

Gene position: 105726-106958 (Clockwise)

Preceding gene: 77456329

Following gene: 77456332

Centisome position: 1.64

GC content: 62.53

Gene sequence:

>1233_bases
ATGACCCAGACTTCGAACAACTCCGATCTGCGTAACGGCCCTGACGCCAACGGCCTGTTTGGCTCGTTCGGCGGCCGTTA
CGTTGCCGAAACCCTGATGCCGTTGATCCTCGACCTGGCCCGCGAATACGAAGCGGCCAAGGAAGATCCGGCGTTCAAAG
AAGAATTGGCCTACTTCCAGCGTGACTATGTCGGACGTCCGAGCCCGCTGTATTTCGCCGAGCGCCTGACCGAGTTCTGC
GGTGGCGCCAAGATCTACCTCAAGCGCGAAGAGCTGAACCACACCGGCGCGCACAAGATCAACAACTGCATCGGCCAGAT
CCTGCTGGCGCGGCGCATGGGCAAAAAACGCATCATCGCCGAGACCGGCGCCGGCATGCACGGCGTGGCCACCGCCACCG
TGGCTGCGCGTTTCGGTCTGGACTGCGTGATCTACATGGGCACCACTGACATCGAACGCCAACAGGCCAATGTGTTCCGC
ATGAAGCTGCTGGGCGCCGAGGTGATCCCGGTGGTCGCCGGCACCGGCACCCTGAAAGACGCAATGAACGAAGCGCTGCG
TGACTGGGTGACCAATGTCGACAGCACCTTCTACCTGATCGGCACCGTGGCCGGTCCGCACCCTTATCCAGCCATGGTTC
GCGACTTCCAGGCGGTGATCGGCAAGGAAACCCGCGATCAGCTGCAAGCCCAGGAAGGCCGCCTGCCGGACAGCCTGGTG
GCGTGCATCGGTGGCGGCTCCAATGCCATGGGCCTGTTCCACCCGTTCCTCGACGACAAGAGCGTTGAAATCATCGGTGT
CGAAGCCGCCGGTTACGGCATCGAGACCGGCAAGCACGCGGCCAGCCTCAACGGCGGTGTACCGGGTGTGCTGCACGGCA
ACCGCACCTTCCTGCTGCAGGACGACGATGGTCAGATCATCGACGCCCACTCGATTTCCGCCGGCCTCGACTATCCGGGC
ATCGGCCCTGAGCACGCGTGGTTGCATGACATCGGCCGCGTCCAGTACACCTCGGTGACCGACGACGAAGCCCTCGCCGC
ATTCCACCAGTGCTGCCGCCTGGAAGGGATCATCCCGGCACTGGAAAGCGCCCACGCCCTGGCCGAAGTATTCAAACGCG
CACCGAAACTGCCGAAGGATCACCTGATGGTGGTCAACCTGTCCGGTCGCGGCGACAAAGACATGCAGACCGTCATGCAC
CACATGGAAACTTCCAAGCAGGAGAAACACTGA

Upstream 100 bases:

>100_bases
TTTTCCTGACAGGTTGTGGCGATCTTATCGGTTTTCAGCCTGGAAGGTCAGGGGTACAGTGAACGCCATTGTCTTCTCGT
GAAATCCGCTGGAGCGAACC

Downstream 100 bases:

>100_bases
TGAGCCGCCTGCAAACCCGCTTTGCCGAACTCAAGGAACAGAACCGCGCCGCCCTGGTGACCTTCGTCACCGCTGGCGAC
CCGGACTACGACACCTCGCT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTQTSNNSDLRNGPDANGLFGSFGGRYVAETLMPLILDLAREYEAAKEDPAFKEELAYFQRDYVGRPSPLYFAERLTEFC
GGAKIYLKREELNHTGAHKINNCIGQILLARRMGKKRIIAETGAGMHGVATATVAARFGLDCVIYMGTTDIERQQANVFR
MKLLGAEVIPVVAGTGTLKDAMNEALRDWVTNVDSTFYLIGTVAGPHPYPAMVRDFQAVIGKETRDQLQAQEGRLPDSLV
ACIGGGSNAMGLFHPFLDDKSVEIIGVEAAGYGIETGKHAASLNGGVPGVLHGNRTFLLQDDDGQIIDAHSISAGLDYPG
IGPEHAWLHDIGRVQYTSVTDDEALAAFHQCCRLEGIIPALESAHALAEVFKRAPKLPKDHLMVVNLSGRGDKDMQTVMH
HMETSKQEKH

Sequences:

>Translated_410_residues
MTQTSNNSDLRNGPDANGLFGSFGGRYVAETLMPLILDLAREYEAAKEDPAFKEELAYFQRDYVGRPSPLYFAERLTEFC
GGAKIYLKREELNHTGAHKINNCIGQILLARRMGKKRIIAETGAGMHGVATATVAARFGLDCVIYMGTTDIERQQANVFR
MKLLGAEVIPVVAGTGTLKDAMNEALRDWVTNVDSTFYLIGTVAGPHPYPAMVRDFQAVIGKETRDQLQAQEGRLPDSLV
ACIGGGSNAMGLFHPFLDDKSVEIIGVEAAGYGIETGKHAASLNGGVPGVLHGNRTFLLQDDDGQIIDAHSISAGLDYPG
IGPEHAWLHDIGRVQYTSVTDDEALAAFHQCCRLEGIIPALESAHALAEVFKRAPKLPKDHLMVVNLSGRGDKDMQTVMH
HMETSKQEKH
>Mature_409_residues
TQTSNNSDLRNGPDANGLFGSFGGRYVAETLMPLILDLAREYEAAKEDPAFKEELAYFQRDYVGRPSPLYFAERLTEFCG
GAKIYLKREELNHTGAHKINNCIGQILLARRMGKKRIIAETGAGMHGVATATVAARFGLDCVIYMGTTDIERQQANVFRM
KLLGAEVIPVVAGTGTLKDAMNEALRDWVTNVDSTFYLIGTVAGPHPYPAMVRDFQAVIGKETRDQLQAQEGRLPDSLVA
CIGGGSNAMGLFHPFLDDKSVEIIGVEAAGYGIETGKHAASLNGGVPGVLHGNRTFLLQDDDGQIIDAHSISAGLDYPGI
GPEHAWLHDIGRVQYTSVTDDEALAAFHQCCRLEGIIPALESAHALAEVFKRAPKLPKDHLMVVNLSGRGDKDMQTVMHH
METSKQEKH

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=383, Percent_Identity=54.3080939947781, Blast_Score=431, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6321412, Length=380, Percent_Identity=55.7894736842105, Blast_Score=456, Evalue=1e-129,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_PSEPF (Q3KK59)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_345836.1
- HSSP:   P0A2K1
- ProteinModelPortal:   Q3KK59
- SMR:   Q3KK59
- STRING:   Q3KK59
- GeneID:   3717802
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_0103
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   PFLU205922:PFL_0103-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 44646; Mature: 44515

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTSNNSDLRNGPDANGLFGSFGGRYVAETLMPLILDLAREYEAAKEDPAFKEELAYFQ
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RDYVGRPSPLYFAERLTEFCGGAKIYLKREELNHTGAHKINNCIGQILLARRMGKKRIIA
HHHCCCCCCHHHHHHHHHHHCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEE
ETGAGMHGVATATVAARFGLDCVIYMGTTDIERQQANVFRMKLLGAEVIPVVAGTGTLKD
CCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCHHEEEEECCCHHHH
AMNEALRDWVTNVDSTFYLIGTVAGPHPYPAMVRDFQAVIGKETRDQLQAQEGRLPDSLV
HHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHH
ACIGGGSNAMGLFHPFLDDKSVEIIGVEAAGYGIETGKHAASLNGGVPGVLHGNRTFLLQ
HEECCCCCCCHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHCCCCCCEEEECCEEEEEE
DDDGQIIDAHSISAGLDYPGIGPEHAWLHDIGRVQYTSVTDDEALAAFHQCCRLEGIIPA
CCCCCEEECCCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCHHH
LESAHALAEVFKRAPKLPKDHLMVVNLSGRGDKDMQTVMHHMETSKQEKH
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQTSNNSDLRNGPDANGLFGSFGGRYVAETLMPLILDLAREYEAAKEDPAFKEELAYFQ
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RDYVGRPSPLYFAERLTEFCGGAKIYLKREELNHTGAHKINNCIGQILLARRMGKKRIIA
HHHCCCCCCHHHHHHHHHHHCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEE
ETGAGMHGVATATVAARFGLDCVIYMGTTDIERQQANVFRMKLLGAEVIPVVAGTGTLKD
CCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCHHEEEEECCCHHHH
AMNEALRDWVTNVDSTFYLIGTVAGPHPYPAMVRDFQAVIGKETRDQLQAQEGRLPDSLV
HHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHH
ACIGGGSNAMGLFHPFLDDKSVEIIGVEAAGYGIETGKHAASLNGGVPGVLHGNRTFLLQ
HEECCCCCCCHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHCCCCCCEEEECCEEEEEE
DDDGQIIDAHSISAGLDYPGIGPEHAWLHDIGRVQYTSVTDDEALAAFHQCCRLEGIIPA
CCCCCEEECCCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCHHH
LESAHALAEVFKRAPKLPKDHLMVVNLSGRGDKDMQTVMHHMETSKQEKH
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA