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The map label for this gene is trpI [H]

Identifier: 77456330

GI number: 77456330

Start: 104696

End: 105613

Strand: Reverse

Name: trpI [H]

Synonym: Pfl01_0102

Alternate gene names: 77456330

Gene position: 105613-104696 (Counterclockwise)

Preceding gene: 77456334

Following gene: 77456328

Centisome position: 1.64

GC content: 65.58

Gene sequence:

>918_bases
ATGAGCCATGACCTTCCCCCGCTGAACGCCCTGCGCGCCTTCGAAGCCACCGCCCGCCTGAACAGCGTGAGCCAGGCTGC
GGAGCAACTGCACGTCACCCACGGCGCGGTCAGCCGCCAGCTCAAGGTGCTGGAAGAGCATCTCGGTGTCAGTCTTTTCG
TCAAGGACGGTCGTGGCCTGAAACTCACAGATGCCGGCATTCGCCTGCGCGATGCCAGCGGCGAGGCGTTCGACCGCTTG
CGCAGCGTTTGCGCCGAACTGACCCAAAGCACCGCCGACGCGCCGTTCGTGCTCGGTTGCTCCGGCAGCCTGCTGGCGCG
CTGGTTCATTCCGCGACTGGGACGCTTGAATGCCGATCTGCCGGACTTGCGTCTGCACCTGTCAGCCGGCGAAGGCGATC
TCGATCCACGACGCCCGGGGCTGGATGCATTGCTGCTGTTCGCCGAGCCGCCATGGCCGGCAGACATGCAAGTCTATGAG
CTGGCCAGCGAGCGGATCGGCCCGGTCATGAGCCCGCTGTTCAGCGGCTACCAGCGCCTGCAATCAGCACCGCCCATCGC
CCTGCTCGACGAACCCTTGCTGCACACGACTTCACGCCCGCAAGCCTGGCCGAGCTGGGCGCAGCAGAGCGGCCTCGACG
CCAAGGCGTTGAAACTGGGGCAAGGTTTCGAACACTTGTATTACTTGCTGGAAGCGGCCGTCGCCGGCTTGGGCGTGGCA
ATCGCGCCAGAACCGCTGGTGACCGAAGACTTGAAGGCCGGACGCCTGGTTGCGCCGTGGGGTTTCAGTGAAACCTCGGC
GCAACTGGCGTTGTGGCTACCCAAGCGCGCCGCAGACGGACGCGCCCGGCAATTGGCGCAGTGGCTCAGAAACGAGCTGC
GCCAGGCGGATCACTCGCCGCGCTTGAACAGCAGATAA

Upstream 100 bases:

>100_bases
CGGATTTCACGAGAAGACAATGGCGTTCACTGTACCCCTGACCTTCCAGGCTGAAAACCGATAAGATCGCCACAACCTGT
CAGGAAAACTCACAGATATC

Downstream 100 bases:

>100_bases
GCGGCCAGCAGACCCAGCGCACCGACCGCCACACCGGCAGTCGTCCACGGGTGTTCCTGCGCGTAATCGCGGGTGGCGAT
CCCGGTCTCGCGGGTTTTGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSHDLPPLNALRAFEATARLNSVSQAAEQLHVTHGAVSRQLKVLEEHLGVSLFVKDGRGLKLTDAGIRLRDASGEAFDRL
RSVCAELTQSTADAPFVLGCSGSLLARWFIPRLGRLNADLPDLRLHLSAGEGDLDPRRPGLDALLLFAEPPWPADMQVYE
LASERIGPVMSPLFSGYQRLQSAPPIALLDEPLLHTTSRPQAWPSWAQQSGLDAKALKLGQGFEHLYYLLEAAVAGLGVA
IAPEPLVTEDLKAGRLVAPWGFSETSAQLALWLPKRAADGRARQLAQWLRNELRQADHSPRLNSR

Sequences:

>Translated_305_residues
MSHDLPPLNALRAFEATARLNSVSQAAEQLHVTHGAVSRQLKVLEEHLGVSLFVKDGRGLKLTDAGIRLRDASGEAFDRL
RSVCAELTQSTADAPFVLGCSGSLLARWFIPRLGRLNADLPDLRLHLSAGEGDLDPRRPGLDALLLFAEPPWPADMQVYE
LASERIGPVMSPLFSGYQRLQSAPPIALLDEPLLHTTSRPQAWPSWAQQSGLDAKALKLGQGFEHLYYLLEAAVAGLGVA
IAPEPLVTEDLKAGRLVAPWGFSETSAQLALWLPKRAADGRARQLAQWLRNELRQADHSPRLNSR
>Mature_304_residues
SHDLPPLNALRAFEATARLNSVSQAAEQLHVTHGAVSRQLKVLEEHLGVSLFVKDGRGLKLTDAGIRLRDASGEAFDRLR
SVCAELTQSTADAPFVLGCSGSLLARWFIPRLGRLNADLPDLRLHLSAGEGDLDPRRPGLDALLLFAEPPWPADMQVYEL
ASERIGPVMSPLFSGYQRLQSAPPIALLDEPLLHTTSRPQAWPSWAQQSGLDAKALKLGQGFEHLYYLLEAAVAGLGVAI
APEPLVTEDLKAGRLVAPWGFSETSAQLALWLPKRAADGRARQLAQWLRNELRQADHSPRLNSR

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=262, Percent_Identity=35.1145038167939, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788706, Length=260, Percent_Identity=30.7692307692308, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=28.5223367697594, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33340; Mature: 33209

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDLPPLNALRAFEATARLNSVSQAAEQLHVTHGAVSRQLKVLEEHLGVSLFVKDGRGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCE
KLTDAGIRLRDASGEAFDRLRSVCAELTQSTADAPFVLGCSGSLLARWFIPRLGRLNADL
EEECCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
PDLRLHLSAGEGDLDPRRPGLDALLLFAEPPWPADMQVYELASERIGPVMSPLFSGYQRL
CCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QSAPPIALLDEPLLHTTSRPQAWPSWAQQSGLDAKALKLGQGFEHLYYLLEAAVAGLGVA
HCCCCCCCCCCCHHHCCCCCCCCCCHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCEE
IAPEPLVTEDLKAGRLVAPWGFSETSAQLALWLPKRAADGRARQLAQWLRNELRQADHSP
ECCCCCCHHHCCCCCEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
RLNSR
CCCCC
>Mature Secondary Structure 
SHDLPPLNALRAFEATARLNSVSQAAEQLHVTHGAVSRQLKVLEEHLGVSLFVKDGRGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCE
KLTDAGIRLRDASGEAFDRLRSVCAELTQSTADAPFVLGCSGSLLARWFIPRLGRLNADL
EEECCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
PDLRLHLSAGEGDLDPRRPGLDALLLFAEPPWPADMQVYELASERIGPVMSPLFSGYQRL
CCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QSAPPIALLDEPLLHTTSRPQAWPSWAQQSGLDAKALKLGQGFEHLYYLLEAAVAGLGVA
HCCCCCCCCCCCHHHCCCCCCCCCCHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCEE
IAPEPLVTEDLKAGRLVAPWGFSETSAQLALWLPKRAADGRARQLAQWLRNELRQADHSP
ECCCCCCHHHCCCCCEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
RLNSR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8423001 [H]